SG177294A1 - Traditional chinese medicine composition to treat bronchial asthma and preparation method thereof - Google Patents
Traditional chinese medicine composition to treat bronchial asthma and preparation method thereof Download PDFInfo
- Publication number
- SG177294A1 SG177294A1 SG2011094356A SG2011094356A SG177294A1 SG 177294 A1 SG177294 A1 SG 177294A1 SG 2011094356 A SG2011094356 A SG 2011094356A SG 2011094356 A SG2011094356 A SG 2011094356A SG 177294 A1 SG177294 A1 SG 177294A1
- Authority
- SG
- Singapore
- Prior art keywords
- parts
- extract
- radix
- chinese medicine
- traditional chinese
- Prior art date
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 219
- 239000003814 drug Substances 0.000 title claims abstract description 132
- 208000006673 asthma Diseases 0.000 title claims abstract description 96
- 238000002360 preparation method Methods 0.000 title claims abstract description 37
- 208000030603 inherited susceptibility to asthma Diseases 0.000 title claims abstract description 23
- 210000000582 semen Anatomy 0.000 claims abstract description 100
- 241000628997 Flos Species 0.000 claims abstract description 46
- 239000000284 extract Substances 0.000 claims description 120
- 238000009472 formulation Methods 0.000 claims description 67
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 64
- 239000000243 solution Substances 0.000 claims description 60
- 239000000843 powder Substances 0.000 claims description 48
- 241000218628 Ginkgo Species 0.000 claims description 45
- 235000011201 Ginkgo Nutrition 0.000 claims description 45
- 235000008100 Ginkgo biloba Nutrition 0.000 claims description 45
- 241000243684 Lumbricus Species 0.000 claims description 44
- 239000008517 radix Trichosanthis Substances 0.000 claims description 44
- UAJTZZNRJCKXJN-UHFFFAOYSA-M sodium;2-dodecoxy-2-oxoethanesulfonate Chemical compound [Na+].CCCCCCCCCCCCOC(=O)CS([O-])(=O)=O UAJTZZNRJCKXJN-UHFFFAOYSA-M 0.000 claims description 43
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 37
- 238000000034 method Methods 0.000 claims description 34
- 239000000463 material Substances 0.000 claims description 29
- 238000010298 pulverizing process Methods 0.000 claims description 19
- 230000002829 reductive effect Effects 0.000 claims description 19
- 239000003826 tablet Substances 0.000 claims description 19
- 239000008187 granular material Substances 0.000 claims description 18
- 238000001914 filtration Methods 0.000 claims description 17
- 238000010438 heat treatment Methods 0.000 claims description 17
- 238000010992 reflux Methods 0.000 claims description 17
- 230000008569 process Effects 0.000 claims description 16
- 239000002552 dosage form Substances 0.000 claims description 9
- 238000002156 mixing Methods 0.000 claims description 8
- 238000005507 spraying Methods 0.000 claims description 7
- 239000002671 adjuvant Substances 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 238000005469 granulation Methods 0.000 claims description 6
- 230000003179 granulation Effects 0.000 claims description 6
- 239000007924 injection Substances 0.000 claims description 6
- 238000002347 injection Methods 0.000 claims description 6
- -1 oral solution Substances 0.000 claims description 6
- 239000007921 spray Substances 0.000 claims description 6
- 239000002775 capsule Substances 0.000 claims description 5
- 239000006187 pill Substances 0.000 claims description 5
- 239000007901 soft capsule Substances 0.000 claims description 5
- 239000000829 suppository Substances 0.000 claims description 5
- 229940098465 tincture Drugs 0.000 claims description 5
- 244000194101 Ginkgo biloba Species 0.000 claims description 4
- 239000006188 syrup Substances 0.000 claims description 4
- 235000020357 syrup Nutrition 0.000 claims description 4
- 229940037003 alum Drugs 0.000 claims description 3
- 239000000499 gel Substances 0.000 claims description 3
- 229940100688 oral solution Drugs 0.000 claims description 3
- 229940079593 drug Drugs 0.000 abstract description 70
- 239000002994 raw material Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 37
- 241000699670 Mus sp. Species 0.000 description 35
- 206010011224 Cough Diseases 0.000 description 34
- 241000700198 Cavia Species 0.000 description 27
- 238000002474 experimental method Methods 0.000 description 20
- 241001465754 Metazoa Species 0.000 description 16
- 108010058846 Ovalbumin Proteins 0.000 description 16
- 229940092253 ovalbumin Drugs 0.000 description 16
- 241000700159 Rattus Species 0.000 description 15
- RAHZWNYVWXNFOC-UHFFFAOYSA-N Sulphur dioxide Chemical compound O=S=O RAHZWNYVWXNFOC-UHFFFAOYSA-N 0.000 description 14
- 206010062717 Increased upper airway secretion Diseases 0.000 description 13
- 206010061218 Inflammation Diseases 0.000 description 13
- 208000026435 phlegm Diseases 0.000 description 13
- 210000002683 foot Anatomy 0.000 description 12
- 230000008961 swelling Effects 0.000 description 12
- 239000011734 sodium Substances 0.000 description 11
- BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 description 10
- 229960003531 phenolsulfonphthalein Drugs 0.000 description 10
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 9
- 206010047924 Wheezing Diseases 0.000 description 9
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 9
- 208000024891 symptom Diseases 0.000 description 9
- 210000003437 trachea Anatomy 0.000 description 9
- NTYJJOPFIAHURM-UHFFFAOYSA-N Histamine Chemical compound NCCC1=CN=CN1 NTYJJOPFIAHURM-UHFFFAOYSA-N 0.000 description 8
- 230000037396 body weight Effects 0.000 description 8
- 230000004054 inflammatory process Effects 0.000 description 8
- 208000037656 Respiratory Sounds Diseases 0.000 description 7
- FQPFAHBPWDRTLU-UHFFFAOYSA-N aminophylline Chemical compound NCCN.O=C1N(C)C(=O)N(C)C2=C1NC=N2.O=C1N(C)C(=O)N(C)C2=C1NC=N2 FQPFAHBPWDRTLU-UHFFFAOYSA-N 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 206010010904 Convulsion Diseases 0.000 description 6
- 235000010418 carrageenan Nutrition 0.000 description 6
- 229920001525 carrageenan Polymers 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- 230000036461 convulsion Effects 0.000 description 6
- 210000003979 eosinophil Anatomy 0.000 description 6
- 238000010171 animal model Methods 0.000 description 5
- 230000001088 anti-asthma Effects 0.000 description 5
- 238000000889 atomisation Methods 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 230000000144 pharmacologic effect Effects 0.000 description 5
- 239000002904 solvent Substances 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 4
- 238000012449 Kunming mouse Methods 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 229920002472 Starch Polymers 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 238000001647 drug administration Methods 0.000 description 4
- 229960001340 histamine Drugs 0.000 description 4
- 230000005764 inhibitory process Effects 0.000 description 4
- 230000002035 prolonged effect Effects 0.000 description 4
- 239000008107 starch Substances 0.000 description 4
- 235000019698 starch Nutrition 0.000 description 4
- 238000007619 statistical method Methods 0.000 description 4
- 206010008479 Chest Pain Diseases 0.000 description 3
- 229920000168 Microcrystalline cellulose Polymers 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- 229960003556 aminophylline Drugs 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 239000000796 flavoring agent Substances 0.000 description 3
- 235000003599 food sweetener Nutrition 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 230000001939 inductive effect Effects 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000008108 microcrystalline cellulose Substances 0.000 description 3
- 235000019813 microcrystalline cellulose Nutrition 0.000 description 3
- 229940016286 microcrystalline cellulose Drugs 0.000 description 3
- 230000003285 pharmacodynamic effect Effects 0.000 description 3
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 3
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 3
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 3
- 239000013641 positive control Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 3
- 239000003765 sweetening agent Substances 0.000 description 3
- 230000001225 therapeutic effect Effects 0.000 description 3
- DKSZLDSPXIWGFO-BLOJGBSASA-N (4r,4ar,7s,7ar,12bs)-9-methoxy-3-methyl-2,4,4a,7,7a,13-hexahydro-1h-4,12-methanobenzofuro[3,2-e]isoquinoline-7-ol;phosphoric acid;hydrate Chemical compound O.OP(O)(O)=O.OP(O)(O)=O.C([C@H]1[C@H](N(CC[C@@]112)C)C3)=C[C@H](O)[C@@H]1OC1=C2C3=CC=C1OC.C([C@H]1[C@H](N(CC[C@@]112)C)C3)=C[C@H](O)[C@@H]1OC1=C2C3=CC=C1OC DKSZLDSPXIWGFO-BLOJGBSASA-N 0.000 description 2
- MPDGHEJMBKOTSU-YKLVYJNSSA-N 18beta-glycyrrhetic acid Chemical compound C([C@H]1C2=CC(=O)[C@H]34)[C@@](C)(C(O)=O)CC[C@]1(C)CC[C@@]2(C)[C@]4(C)CC[C@@H]1[C@]3(C)CC[C@H](O)C1(C)C MPDGHEJMBKOTSU-YKLVYJNSSA-N 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical group [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 2
- 206010018691 Granuloma Diseases 0.000 description 2
- 206010020751 Hypersensitivity Diseases 0.000 description 2
- 208000019693 Lung disease Diseases 0.000 description 2
- 229920000881 Modified starch Polymers 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 2
- JUGOREOARAHOCO-UHFFFAOYSA-M acetylcholine chloride Chemical compound [Cl-].CC(=O)OCC[N+](C)(C)C JUGOREOARAHOCO-UHFFFAOYSA-M 0.000 description 2
- 229960004266 acetylcholine chloride Drugs 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 208000037883 airway inflammation Diseases 0.000 description 2
- 208000026935 allergic disease Diseases 0.000 description 2
- 230000007815 allergy Effects 0.000 description 2
- 235000019270 ammonium chloride Nutrition 0.000 description 2
- 239000005557 antagonist Substances 0.000 description 2
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 2
- 239000011230 binding agent Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000001684 chronic effect Effects 0.000 description 2
- OROGSEYTTFOCAN-DNJOTXNNSA-N codeine Chemical compound C([C@H]1[C@H](N(CC[C@@]112)C)C3)=C[C@H](O)[C@@H]1OC1=C2C3=CC=C1OC OROGSEYTTFOCAN-DNJOTXNNSA-N 0.000 description 2
- 229960004415 codeine phosphate Drugs 0.000 description 2
- 230000008602 contraction Effects 0.000 description 2
- 230000002354 daily effect Effects 0.000 description 2
- 229960003957 dexamethasone Drugs 0.000 description 2
- UREBDLICKHMUKA-CXSFZGCWSA-N dexamethasone Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@]2(F)[C@@H]1[C@@H]1C[C@@H](C)[C@@](C(=O)CO)(O)[C@@]1(C)C[C@@H]2O UREBDLICKHMUKA-CXSFZGCWSA-N 0.000 description 2
- 229940000406 drug candidate Drugs 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 239000003777 experimental drug Substances 0.000 description 2
- 238000000556 factor analysis Methods 0.000 description 2
- 239000000945 filler Substances 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 235000013355 food flavoring agent Nutrition 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 229960001660 histamine phosphate Drugs 0.000 description 2
- ZHIBQGJKHVBLJJ-UHFFFAOYSA-N histamine phosphate Chemical compound OP(O)(O)=O.OP(O)(O)=O.NCCC1=CNC=N1 ZHIBQGJKHVBLJJ-UHFFFAOYSA-N 0.000 description 2
- 210000003630 histaminocyte Anatomy 0.000 description 2
- 239000001866 hydroxypropyl methyl cellulose Substances 0.000 description 2
- 235000010979 hydroxypropyl methyl cellulose Nutrition 0.000 description 2
- 229920003088 hydroxypropyl methyl cellulose Polymers 0.000 description 2
- UFVKGYZPFZQRLF-UHFFFAOYSA-N hydroxypropyl methyl cellulose Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(O)C(CO)O3)O)C(CO)O2)O)C(CO)O1 UFVKGYZPFZQRLF-UHFFFAOYSA-N 0.000 description 2
- 230000001965 increasing effect Effects 0.000 description 2
- 230000008595 infiltration Effects 0.000 description 2
- 238000001764 infiltration Methods 0.000 description 2
- 230000002757 inflammatory effect Effects 0.000 description 2
- 239000007928 intraperitoneal injection Substances 0.000 description 2
- 239000000314 lubricant Substances 0.000 description 2
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- OXNIZHLAWKMVMX-UHFFFAOYSA-N picric acid Chemical compound OC1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-N 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- 238000007670 refining Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000000638 solvent extraction Methods 0.000 description 2
- 239000000375 suspending agent Substances 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 108010011485 Aspartame Proteins 0.000 description 1
- 206010003645 Atopy Diseases 0.000 description 1
- 239000005711 Benzoic acid Substances 0.000 description 1
- 229920001661 Chitosan Polymers 0.000 description 1
- 208000017667 Chronic Disease Diseases 0.000 description 1
- 229920002785 Croscarmellose sodium Polymers 0.000 description 1
- UDIPTWFVPPPURJ-UHFFFAOYSA-M Cyclamate Chemical compound [Na+].[O-]S(=O)(=O)NC1CCCCC1 UDIPTWFVPPPURJ-UHFFFAOYSA-M 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 208000000059 Dyspnea Diseases 0.000 description 1
- 206010013975 Dyspnoeas Diseases 0.000 description 1
- 206010014561 Emphysema Diseases 0.000 description 1
- 241001411320 Eriogonum inflatum Species 0.000 description 1
- OTMSDBZUPAUEDD-UHFFFAOYSA-N Ethane Chemical compound CC OTMSDBZUPAUEDD-UHFFFAOYSA-N 0.000 description 1
- MPDGHEJMBKOTSU-UHFFFAOYSA-N Glycyrrhetinsaeure Natural products C12C(=O)C=C3C4CC(C)(C(O)=O)CCC4(C)CCC3(C)C1(C)CCC1C2(C)CCC(O)C1(C)C MPDGHEJMBKOTSU-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 240000000233 Melia azedarach Species 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 241001522129 Pinellia Species 0.000 description 1
- 208000002200 Respiratory Hypersensitivity Diseases 0.000 description 1
- 206010070834 Sensitisation Diseases 0.000 description 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 231100000215 acute (single dose) toxicity testing Toxicity 0.000 description 1
- 238000011047 acute toxicity test Methods 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 239000000556 agonist Substances 0.000 description 1
- 230000036428 airway hyperreactivity Effects 0.000 description 1
- 230000010085 airway hyperresponsiveness Effects 0.000 description 1
- 239000013566 allergen Substances 0.000 description 1
- 230000000172 allergic effect Effects 0.000 description 1
- WNROFYMDJYEPJX-UHFFFAOYSA-K aluminium hydroxide Chemical compound [OH-].[OH-].[OH-].[Al+3] WNROFYMDJYEPJX-UHFFFAOYSA-K 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003266 anti-allergic effect Effects 0.000 description 1
- 230000001078 anti-cholinergic effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 239000000924 antiasthmatic agent Substances 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 239000000605 aspartame Substances 0.000 description 1
- 235000010357 aspartame Nutrition 0.000 description 1
- IAOZJIPTCAWIRG-QWRGUYRKSA-N aspartame Chemical compound OC(=O)C[C@H](N)C(=O)N[C@H](C(=O)OC)CC1=CC=CC=C1 IAOZJIPTCAWIRG-QWRGUYRKSA-N 0.000 description 1
- 229960003438 aspartame Drugs 0.000 description 1
- 208000010668 atopic eczema Diseases 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 229960002233 benzalkonium bromide Drugs 0.000 description 1
- KHSLHYAUZSPBIU-UHFFFAOYSA-M benzododecinium bromide Chemical compound [Br-].CCCCCCCCCCCC[N+](C)(C)CC1=CC=CC=C1 KHSLHYAUZSPBIU-UHFFFAOYSA-M 0.000 description 1
- 235000010233 benzoic acid Nutrition 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 210000000621 bronchi Anatomy 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 229960002152 chlorhexidine acetate Drugs 0.000 description 1
- 208000037976 chronic inflammation Diseases 0.000 description 1
- 230000006020 chronic inflammation Effects 0.000 description 1
- 230000007012 clinical effect Effects 0.000 description 1
- 229960004126 codeine Drugs 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 239000001767 crosslinked sodium carboxy methyl cellulose Substances 0.000 description 1
- 235000010947 crosslinked sodium carboxy methyl cellulose Nutrition 0.000 description 1
- 239000000625 cyclamic acid and its Na and Ca salt Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- GRWZHXKQBITJKP-UHFFFAOYSA-L dithionite(2-) Chemical compound [O-]S(=O)S([O-])=O GRWZHXKQBITJKP-UHFFFAOYSA-L 0.000 description 1
- 229920001971 elastomer Polymers 0.000 description 1
- 229960003720 enoxolone Drugs 0.000 description 1
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 description 1
- 229940044949 eucalyptus oil Drugs 0.000 description 1
- 239000010642 eucalyptus oil Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 238000001415 gene therapy Methods 0.000 description 1
- 230000008570 general process Effects 0.000 description 1
- 238000011554 guinea pig model Methods 0.000 description 1
- 238000011597 hartley guinea pig Methods 0.000 description 1
- 239000006049 herbal material Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- OROGSEYTTFOCAN-UHFFFAOYSA-N hydrocodone Natural products C1C(N(CCC234)C)C2C=CC(O)C3OC2=C4C1=CC=C2OC OROGSEYTTFOCAN-UHFFFAOYSA-N 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000007365 immunoregulation Effects 0.000 description 1
- 238000009169 immunotherapy Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 210000004969 inflammatory cell Anatomy 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229940125369 inhaled corticosteroids Drugs 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 239000007927 intramuscular injection Substances 0.000 description 1
- 238000010255 intramuscular injection Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- VNYSSYRCGWBHLG-AMOLWHMGSA-M leukotriene B4(1-) Chemical compound CCCCC\C=C/C[C@@H](O)\C=C\C=C\C=C/[C@@H](O)CCCC([O-])=O VNYSSYRCGWBHLG-AMOLWHMGSA-M 0.000 description 1
- 239000003199 leukotriene receptor blocking agent Substances 0.000 description 1
- 231100001252 long-term toxicity Toxicity 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229940031703 low substituted hydroxypropyl cellulose Drugs 0.000 description 1
- 210000003141 lower extremity Anatomy 0.000 description 1
- 235000019359 magnesium stearate Nutrition 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 230000000414 obstructive effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- MCSINKKTEDDPNK-UHFFFAOYSA-N propyl propionate Chemical compound CCCOC(=O)CC MCSINKKTEDDPNK-UHFFFAOYSA-N 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 235000019204 saccharin Nutrition 0.000 description 1
- CVHZOJJKTDOEJC-UHFFFAOYSA-N saccharin Chemical compound C1=CC=C2C(=O)NS(=O)(=O)C2=C1 CVHZOJJKTDOEJC-UHFFFAOYSA-N 0.000 description 1
- 229940081974 saccharin Drugs 0.000 description 1
- 239000000901 saccharin and its Na,K and Ca salt Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
- 230000001235 sensitizing effect Effects 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 208000019116 sleep disease Diseases 0.000 description 1
- 235000015424 sodium Nutrition 0.000 description 1
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 description 1
- 235000010234 sodium benzoate Nutrition 0.000 description 1
- 239000004299 sodium benzoate Substances 0.000 description 1
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 1
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 1
- 229960001462 sodium cyclamate Drugs 0.000 description 1
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 1
- 235000010199 sorbic acid Nutrition 0.000 description 1
- 239000004334 sorbic acid Substances 0.000 description 1
- 229940075582 sorbic acid Drugs 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229960004793 sucrose Drugs 0.000 description 1
- 238000002636 symptomatic treatment Methods 0.000 description 1
- 238000012353 t test Methods 0.000 description 1
- 239000000454 talc Substances 0.000 description 1
- 229910052623 talc Inorganic materials 0.000 description 1
- 235000012222 talc Nutrition 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 210000000534 thyroid cartilage Anatomy 0.000 description 1
- 210000005090 tracheal smooth muscle Anatomy 0.000 description 1
- 239000006200 vaporizer Substances 0.000 description 1
- 229940126673 western medicines Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/16—Ginkgophyta, e.g. Ginkgoaceae (Ginkgo family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/62—Leeches; Worms, e.g. cestodes, tapeworms, nematodes, roundworms, earth worms, ascarids, filarias, hookworms, trichinella or taenia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/17—Gnetophyta, e.g. Ephedraceae (Mormon-tea family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/42—Cucurbitaceae (Cucumber family)
- A61K36/428—Trichosanthes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/535—Perilla (beefsteak plant)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/539—Scutellaria (skullcap)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
- A61K36/605—Morus (mulberry)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
- A61K36/634—Forsythia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/65—Paeoniaceae (Peony family), e.g. Chinese peony
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/736—Prunus, e.g. plum, cherry, peach, apricot or almond
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/78—Saururaceae (Lizard's-tail family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/888—Araceae (Arum family), e.g. caladium, calla lily or skunk cabbage
- A61K36/8888—Pinellia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
- A61P11/06—Antiasthmatics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
- A61P11/08—Bronchodilators
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
- Epidemiology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Biotechnology (AREA)
- Alternative & Traditional Medicine (AREA)
- Mycology (AREA)
- Pulmonology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Neurosurgery (AREA)
- Insects & Arthropods (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
- Medicinal Preparation (AREA)
Abstract
Traditional Chinese Medicine Composition to Treat Bronchial Asthma andThe present invention provides a traditional Chinese medicine composition for treating Bronchial Asthma and preparation method thereof. The traditional Chinese medicine composition is prepared from pure plant raw material drugs including Herba Ephedrae, SemenGinkgo , Cortex Mori, Radix Scutellariae, Semen Armeniacae Amarum, Rhizoma Pinelliae,Fructus Perillae, Flos Farfarae and so on. The traditional Chinese medicine composition is effective in treating Bronchial asthma.
Description
Traditional Chinese Medicine Composition to Treat Bronchial Asthma and Preparation
Method thereof
The present invention relates to a traditional Chinese medicine composition for treating bronchial asthma and preparation method thereof, and it belongs to the field of Chinese herbal medical art.
Bronchial asthma Chereinafter “asthma”), is a common chronic lung disease. At present, about 200 million people worldwide suffer from asthma, and there are about 20 million asthma patients in China. The data from five major Chinese cities show that asthma incidence rate of 3-14 years old students is 3% to 5%. Generally, it is believed that the incidence rate is higher in children than in adults, and almost same with adult men and women. About 40% of patients have family history. A survey shows that 33% of asthma patients required hospitalization or emergency treatment because of asthma in the past year, 58% of the patients incurred time loss of work due to asthma, 79% of asthma patients could not perform normal sports and leisure activities, 63% of asthma patients had to change their original way of living, 68% of asthma patients suffered sleep disorders because of asthma, and 74% of asthma patients could not perform normal physical labor.
Asthma is a modern disease. Its incidence rate will not decrease with the improvement of people's health and living standards. Instead, the incidence rate of asthma keeps increasing.
In the past 20 years, people have been actively seeking effective methods of preventing and treating asthma, which is a subject of tireless exploration of the medical profession. In China as well as abroad, commonly used western drugs to treat this disease are from the following categories: inhaled corticosteroids, $2 agonists, inhaled anticholinergic drugs, theophyllines, leukotriene antagonists, antagonists of other inflammatory mediators and cytokine antagonists, bone marrow eosinophil progenitor cell inhibitors, and allergen-specific immunotherapy and gene therapy. In recent years, these drugs achieved some clinical effects. However, the main aim of these drugs is to ease a series of episodes of bronchial inflammation and symptoms. They still do not solve the problems generally concerned by the respiratory professionals, which are the reduction in frequency and long-term control of clinical symptoms. In addition, there are many more problems in the treatment of asthma patients, such as the length of the treatment, when to reduce the dosage, how to reduce the dosage, when to take the patients off the medicine, which method or program can achieve the goal of controlling asthma, and which is more effective and safe for asthma patients, These problems not only challenge the clinicians, but also add more difficulties to continued treatment of the patients. Meanwhile, some adverse side effects of the western medicines have seriously affected continued treatment of the patients. According to a survey, nearly half of the asthma patients in China do not have not an ideal control of their asthma, and most asthma patients in the basic level and rural areas do not even have an effective control of their disease.. It is a long way to achieve the goal of generally improving the effectiveness of asthma prevention and treatment methods and medicines.
Traditional Chinese medicine has over thousands of years of history in the prevention and treatment of bronchial asthma. Ancient doctors had constantly explored treatment principles, methods, prescriptions and medicines for asthma, and had discovered a lot of effective prescriptions..Pharmacological studies have shown that treating asthma with traditional Chinese medicine has comprehensive effects of regulating immune system, anti-allergy, relieving asthma, preventing cough, expelling phlegm, anti-inflammatory and so on. Traditional Chinese medicine can reduce airway inflammation and airway hyperresponsiveness. Clinical studies have shown that the treatment with traditional Chinese medicine has less adverse effects than western medicine, and can significantly relieve symptoms such as wheezing, chest distress, coughing, phlegm and so on. Most patients can enter remission phase of asthma during the treatment period, or reduce the dose of hormone, and improve their quality of life.
Dingchuan decoction, recorded in "She Sheng Zhong Miao Fang" (a traditional Chinese medicine formulations book), is composed of honey-baking Herba Ephedrae, stir-fry Semen
Ginkgo, Cortex Mori, radix scutellariae, Semen Armeniacae Amarum, Rhizoma Pinelliae, Fructus
Perillae, radix paeoniae rubra, lumbricus, Periostracum Cicadae, radix trichosanthis and Fructus
Forsythiae. It is a famous prescription for treating asthma. Experiments showed that Dingchuan decoction has a protective effect on experimental asthma induced by histamine and acetyl choline chloride spray, and can antagonize histamine-induced contraction of isolated tracheal smooth muscle in guinea pigs [Li, zhengmu et al., pharmacology research of Dingchuan decoction in treating bronchial asthma. Traditional Chinese Medicinal Materials. 1999.22 (8):411]. It can further promote the secretion of phenol red in mice respiratory tract. These results prove, from a pharmacological perspective, that Dingchuan decoction has a satisfactory effect on relieving asthma and expelling phlegm.
The traditional Chinese medicine composition in this invention is made from pure natural herbal materials, and comprises Herba Ephedrae, Cortex Mori, Radix Scutellariae, Semen
Armeniacae Amarum, Rhizoma Pinelline, Fructus Perillae, Flos Farfarae and so on. This composition significantly relieves the symptoms of bronchial asthma, such as wheezing, chest distress, coughing, stethocatharsis, and its effect is clearly better than that of Dingchuan decoction.
Content of the Invention
One aspect of the present invention is to provide a traditional Chinese medicine composition for treating bronchial asthma.
Another aspect of the present invention is to provide a preparation method of the traditional
Chinese medicine composition for treating bronchial asthma,
A traditional Chinese medicine composition of present invention is prepared from the following raw medicinal materials by weight
Herba Ephedrae 40-90 parts, Cortex Mori 136-300 parts, Lumbricus 146-300 parts,
Semen Ginkgo 46-151 parts, Flos Farfarae 46-151 parts, Rhizoma Pinelliae 46-151 parts,
Fructus Perillae 46-151 parts, Radix Paeconiae Rubra 146-300 parts,
Semen Armeniacae Amarum 46-151 parts, Radix Scutellariae 46-151 parts,
Periostracum Cicadae 46-151 parts, Herba Houttuyniae 146-300 parts,
Radix Trichosanthis 146-300 parts, and Fructus Forsythiae 46-151 parts.
Another traditional Chinese medicine composition of present invention is prepared from the following raw medicinal materials by weight :
Herba Ephedrae 77-88 parts, Cortex Mori 136-165 parts, Lumbricus 255-300 parts,
Semen Ginkgo 46-65 parts, Flos Farfarae 110-151 parts, Rhizoma Pinelliae 46-65 parts,
Fructus Perillae 105-151 parts, Radix Paconiae Rubra 146-165 parts,
Semen Armeniacae Amarum 46-65 parts, Radix Scutellariae 103-151 parts,
Periostracum Cicadae 46-65 parts, Herba Houttuyniae 255-300 parts,
Radix Trichosanthis 255-300 parts, and Fructus Forsythiae 103-151 parts.
Preferablely, a traditional Chinese medicine composition of present invention is prepared from following raw medicinal materials by weight :
Herba Ephedrae 50-76 parts, Cortex Mori 166-253 parts, Lumbricus 166-253 parts,
Semen Ginkgo 66-101 parts, Flos Farfarae 66-101 parts, Rhizoma Pinelliae 66-101 parts,
Fructus Perillae 66-101 parts, Radix Paconiae Rubra 166-253 parts,
Semen Armeniacae Amarum 66-101 parts, Radix Scutellariae 66-101 parts,
Periostracum Cicadae 66-101 parts, Herba Houttuyniae 166-253 parts,
Radix Trichosanthis 166-253 parts, and Fructus Forsythiae66-101 parts.
Preferablely, a traditional Chinese medicine composition of present invention is prepared from the following raw medicinal materials by weight:
Herba Ephedrae 50 parts, Cortex Mori 253 parts, Lumbricus 166 parts,
Semen Ginkgo 66 parts, Flos Farfarag 101 parts, Rhizoma Pinelliae 101parts,
Fructus Perillae 66 parts, Radix Paconiae Rubra 166 parts,
Semen Armeniacae Amarum 101 parts, Radix Scutellariae 66 parts,
Periostracum Cicadae 66 parts, Herba Houttuyniae 253 parts,
Radix Trichosanthis 253 parts, and Fructus Forsythiae 66 parts.
Preferablely, a traditional Chinese medicine composition of present invention is prepared from the following raw medicinal materials by weight:
Herba Ephedrae 76 parts, Cortex Mori 166 parts, Lumbricus 253parts,
Semen Ginkgo 101 parts, Flos Farfarae 66 parts, Rhizoma Pinelliae 66parts,
Fructus Perillae 101 parts, Radix Paeoniae Rubra 253 parts,
Semen Armeniacae Amarum 66 parts, Radix Scutellariac 101 parts,
Periostracum Cicadae 101 parts, Herba Houttuyniae 166 parts,
Radix Trichosanthis 166 parts, and Fructus Forsythiae 101 parts.
Preferablely, a traditional Chinese medicine composition of present invention is prepared from the following raw medicinal materials by weight:
Herba Ephedrae 70 parts, Cortex Mori 241 parts, Lumbricus 245parts,
Semen Ginkgo 98 parts, Flos Farfarae 94 parts, Rhizoma Pinelliae 95 parts,
Fructus Perillae 94 parts, Radix Paconiae Rubra 230 parts,
Semen Armeniacae Amarum 92 parts, Radix Scutellariae 91 parts,
Periostracum Cicadae 97 parts, Herba Houttuyniae 237 parts,
Radix Trichosanthis 231 parts, and Fructus Forsythiae 91parts.
Preferablely, a traditional Chinese medicine composition of present invention is prepared from the following raw medicinal materials by weight:
Herba Ephedrae 63 parts, Cortex Mori 210 parts, Lumbricus 210parts,
Semen Ginkgo 84 parts, Flos Farfarae 84 parts, Rhizoma Pinelliae 84 parts,
Fructus Periliae 84 parts, Radix Paeoniae Rubra 210 parts,
Semen Armeniacae Amarum 84 parts, Radix Scutellariac 84 parts,
Periostracum Cicadae 84 parts, Herba Houttuyniae 210 parts,
Radix Trichosanthis 210 parts, and Fructus Forsythiae 84 parts.
Preferablely, a traditional Chinese medicine composition of present invention is prepared from the following raw medicinal materials by weight:
Herba Ephedrae 61 parts, Cortex Mori 198 parts, Lumbricus 223parts,
Semen Ginkgo 78 parts, Flos Farfarae 92 parts, Rhizoma Pinelliae 73 parts,
Fructus Perillae 85 parts, Radix Paeoniae Rubra 188 parts,
Semen Armeniacae Amarum 87 parts, Radix Scutellariae 88 parts,
Periostracum Cicadae 79 parts, Herba Houttuyniae 221 parts,
Radix Trichosanthis 218 parts, and Fructus Forsythiae 81 parts.
Preferablely, the raw medicinal materials of the traditional Chinese medicine composition in the present invention are: Herba Ephedrae is honey-baking Herba Ephedrae, Semen Gingko is stir-fry Semen Gingko, Rhizoma Pinelliae is alum processed Rhizoma Pinelliae, Fructus Perillae is stir-fry Fructus Periilae, and Semen Armeniacae Amarum is stir-fry Semen Armeniacae
Amarum,
The raw medicinal materials of present invention can be substituted by traditional Chinese medicinal materials having same or similar functions, and these materials can be processed in accordance with the "National Standard of Traditional Chinese Medicine Processing" or "Great
Dictionary of Traditional Chinese medicine".
The traditional Chinese medicine composition of present invention is prepared from raw medicinal materials, and “prepared” can be understood as any general process used in the preparation of tradition Chinese medicine, such as preparing traditional Chinese medicine composition after direct pulverizing or concentrating the extract from conventional solvent extraction. It also includes additional refining methods after the conventional solvent extraction, such as refining through the macroporous resin column.
Furthermore, the inventive composition can be prepared in various dosage forms, including capsule, tablet, powder, oral liquid, soft capsule, pill, tincture, symp, suppository, gel and spray.
Preferablely, the traditional Chinese medicine composition of present invention is prepared using the following method: a) taking Radix Scutellariae and 1/3—3/4 amount of Rhizoma Pinelliae according to the formulation proportion, pulverizing them into fine powder for later use; b) taking of the relative amounts of Herba Ephedrae, Semen Ginkgo, Cortex Mori, Flos
Farfarae, Fructus Perillae, Semen Armeniacae Amarum and Herba Houttuyniae according to the formulation and the rest of Rhizoma Pinelliae, adding 6-10 times amount of 50-80% ethanol, heating and refluxing extract thrice, 1-3 hours each time, recovering ethanol from extract solution under reduced pressure, and concentrating the solution to obtain the extract with a relative density of 1.05 -1,10 at 60°C for later use; c) taking the relative amount of Lumbricus, Radix Paconiae Rubra, Periostracuimn Cicadae,
Radix Trichosanthis and Fructus Forsythiae according to the formulation, adding 7- 10 times amount of water, decocting twice, 1-4 hours each time, filtering the water decoction, combining decoctions, concentrating the solution to obtain the extract with a relative density of 1.05 - 1.10 at 60°C, and combining the extract with extract of step b) for later use; d) mixing the fine powder of step a) with the combined extract of step c) to obtain the traditional Chinese medicine composition. The composition can further be prepared to clinical acceptable dosage forms, including capsule, tablet, powder, oral administration liquid, soft capsule, pill, tincture, syrup, suppository, gel and spray, through routine processes.
As used here, “adding X times amount of * solvent refers to weight/volume ratio, for example, “adding 6-8 times amount of solvent in raw medicinal materials” refers to adding 6-8 liters of solvent to 1 kilogram of raw medicinal materials.
In order to achieve the desired dosage forms, it is necessary to add pharmaceutically acceptable excipients in the preparation of these forms, such as fillers, disintegrating agents, lubricants,
suspending agents, binders, sweeteners, flavoring agents, preservatives, eic. Fillers include starch, pregelatinized starch, lactose, mannitol, chitosan, microcrystalline cellulose, sucrose, etc.
Disintegrating agents include starch, pregelatinized starch, microcrystalline cellulose, sodium carboxymethyl starch, cross-linked polyvinylpyrrolidone, low substituted hydroxypropyl cellulose, crosslinked sodium carboxymethyl cellulose, etc. Lubricants include magnesium stearate, sodium lauryl sulfate, talc, silica, etc. Suspending agents include polyvinyl pyrrolidone, microcrystalline cellulose, sucrose, agar, hydroxypropyl methyl cellulose, etc. Binders include starch, polyvinylpyrrolidone, hydroxypropyl methyl cellulose, etc. Sweeteners include saccharin, aspartame, sucrose, sodium cyclamate, glycyrrhetinic acid, etc. Flavoring agents include sweeteners and various flavors. Preservatives including: nipagins, benzoic acid, sodium benzoate, sorbic acid and its salts, benzalkonium bromide, Chlorhexidine Acetate, eucalyptus oil, etc.
Another embodiment of the present invention provides a preparation method of preparing tablet dosage form of the traditional Chinese medicine composition. This method includes the following steps: a) taking Radix Scutellariae and half amount of Rhizoma Pinelliae according to the formulation proportion, pulverizing them into fine powder for later use; b) taking the relative amounts of Herba Ephedrae, Semen Ginkgo, Cortex Mori, Flos Farfarae,
Fructus Perillae, Semen Armeniacae Amarum and Herba Houttuyniae according to the formulation and the rest of Rhizoma Pinelliae in proportion, adding 6-10 times amount of 50-80% ethanol, heating and refluxing extract thrice, 1-3 hours each time, recovering ethanol from extract solution under reduced pressure, concentrating the solution to obtain the extract with a relative density of 1.05-1.10 at 60°C for later use; ¢) taking the relative amounts of Lumbricus, Radix Paeoniae Rubra, Periostracum Cicadae,
Radix Trichosanthis and Fructus Forsythiae according to the formulation, adding 7- 10 times amount of water, decocting twice, 1-4 hours each time, filtering the water decoction, combining decoctions, concentrating the solution to obtain the extract with a relative density of 1.05 - 1.10 at 60°C, and combining the extract with extract of step b) for later use; d) Mixing the fine powder of step a) and the extract of step c), adding an appropriate amount of adjuvant into the mixture, and spraying granulation to obtain granules and sifting the granules through a mesh after drying, standing by. ; and e) preparing the granules of step d) to a tablet dosage form through routine processes.
Preferably, the preparation method of a tablet dosage form of the traditional Chinese medicine composition includes the following steps: a) taking Radix Scutellariae and half amount of Rhizoma Pinelliae according to the formulation proportion, pulverizing them into fine powder for later use; b) taking the relative amounts of Herba Ephedrae, Semen Ginkgo, Cortex Mori, Flos Farfarae,
Fructus Perillae, Semen Armeniacae Amarum and Herba Houttuyniae according to the formulation and the rest of Rhizoma Pinelliae in proportion, adding 8 times amount of 60% ethanol, heating and refluxing extract thrice, 1.5 hours each time, recovering ethanol from extract solution under reduced pressure, concentrating the solution to obtain the extract with a relative density of 1.05 - 1.10 at 60°C for later use; ¢) taking the relative amounts of Lumbricus, Radix Paeoniae Rubra, Periostracum Cicadae,
Radix Trichosanthis and Fructus Forsythiae according the formulation, adding 9 times amount of water, decocting twice, 2 hours each time, filtering the water decoction, combining decoctions, concentrating the solution to obtain the extract with a relative density of 1.05 - 1.10 at 60°C, and combining the extract with the extract of step b) for later use; d} Mixing the fine powder of step a) and the extract of step ¢), adding an appropriate amount of adjuvant into the mixture, and spraying granulation to obtain granules and sifting the granules through a mesh after drying, standing by. ; and; e) preparing the granules of step d) to a tablet dosage form through routine processes.
According to total weight of the raw medicinal materials of the traditional Chinese medicine composition of the present invention, the dosage of the composition is 15 - 30 g/day, and it can be taken once daily or 2-4 times daily; preferably the dosage is 22 g / day, and to be taken three times a day.
To confirm the activity of the inventive traditional Chinese medicine composition (hereinafter “the inventive composition”), the tablet dosage form of the inventive composition prepared using method disclosed in Example 1 was crushed into powder, and pharmacological tests were conducted to demonstrate the inventive composition’s effect on treating bronchial asthma. Also, the therapeutic effect of the primary pharmacodynamic part of the tablet was compared to that of
Dingchuan decoction, prepared according to a published method: adding 9 grams of each of Herba
Ephedrae, Flos Farfarae, Semen Armeniacae Amarum and Rhizoma Pinellia, 12 grams of Semen
Ginkgo (stir-fry to yellowish), 6 grams of each of Fructus Perillae, Cortex Mori and Radix
Scutellariae, and 3 grams of Radix Glycyrrhizae to water and decocting the ingredients to obtain the Dingchuan decoction. [Ha, xiaobo and Xiao, xian. Talking about Dingchuan decoction , KAI
JUAN YOU YLQIU YI WEN YAO, 2001 (11):21.] The experiment confirms that this inventive composition is more therapeutically effective than Dingchuan decoction in treating asthma.
It has been confirmed that this inventive composition has good therapeutic effect on treating bronchial asthma by relieving asthma and coughing, resolving phlegm, anti-inflammation and so on. Bronchial asthma is a common chronic lung disease. This inventive composition has a wide safety range, plays a regulatory role from several aspects, and treats both the symptoms and the causes of the disease. It not only avoids the side effects of single targeting associated with western medicine, but also treats chronic diseases for a long period. Acute toxicity test results indicate that this inventive composition exhibited no significant toxicity in mice, which were intragastrically administered with 254.4g crude drug/kg at peak concentration for 3 times a day, and observed continuously for 14 days. This dosage is equivalent to 687.57 times of human clinical dosage. The experimental results of the long-term toxicity test in rats also show that this inventive composition exhibited no significant toxicity in rats administered with dosages of 22g, 11g, and 5.5g crude drug/kg for a long period (26 weeks).
The following experiments and examples are used to further illustrate but not limit to the present invention.
Experiment 1: Anti-asthmatic experiment 1. The effect of the inventive composition on early-phase reaction of bronchial asthma 1. Experimental material 1.1 Animals: pure-white healthy Hartley guinea pigs with a body mass of 200 25g, half males and half females, were purchased from Experimental Animal Center of National Institute for the
Control of Pharmaceutical and Biological Products, Certification number is SCXK (BJ)2002-0010.
1.2 Drugs and reagents : (1) Testing drugs: The inventive composition drug, batch number: 20060801, was provided by
Shijiazhuang Yiling Pharmaceutical Co., Ltd. 1 gram of the final composition drug corresponds to 4.5g of crude drug. The human clinical dosage of the drug is 22g crude drug/day. The drug was combined with 0.5% CMC — Na (sodium carboxymethyl cellulose} to prepare a solution of required concentration before use. (2) Positive control drug: Aminophylline tablet suitable for treating bronchus asthma and obstructive pulmonary emphysema was produced by Peking Zizhu Pharmaceutical Co., Ltd, batch number: 20061103. ] (3) Dingchuan decoction: provided by Shijiazhuang Yiling Pharmaceutical Co., Lid. The dosage for human use is 69g crude drug/day. The drug is combined with 0.5%CMC - Na (sodium cellulose glycolate) to prepare a solution with required concentration before use. As used here, “......g crude drug/day” means the dosage of the raw medicinal materials per day. (4) Ovalbumin: Product of Sigma Company. (5) Acecolex: Product of Sigma Company. 1.3 Apparatus: Yadu medical vaporizer, model YC-Y800B, produced by Beijing Yadu
Technology Co., Ltd. 2. Methods and results 1. Groups and drug administration of the animals:
After grouping, 80 guinea pigs were put into an air-tight organic glass cover (4L) in batches. An asthma inducing solution (isometric intermixture of 2% acecolex and 0.1% histamine phosphate) was atomized and sprayed into the glass cover by ultrasonic atomizer at a rate of 15 seconds/time, where the atomization volume was 4ml/min and atomization particle diameter was 1-5m. After atomization, the latent period of wheezing convulsion of guinea pigs within 6 min was observed.
Guinea pigs having a latent period of wheezing convulsion exceeding 120 seconds were excluded from further experiment. 66 eligible guinea pigs were selected and randomly divided into 6 groups: asthma model group, aminophylline group, Dingchuan decoction group, high, medium and low dosages of the inventive composition groups. Asthma model group was intragastrically administrated with same volume of solvent; aminophylline group was intragastrically administrated with aminophylline at 0.06g/kg (corresponds to 7 times of human clinical dosage);
Dingchuan decoction group is intragastrically administrated 8.1 g/kg of the decoction (correspond to 7 times of human clinical dosage); high, medium and low dosages of the inventive composition groups were intragastrically administrated with the composition at 5.1g/kg, 2.6g/kg, and 1.3g/kg, respectively (correspond to 14, 7 and 3.5 times of human clinical dosage, respectively). Each group was administered with the respective drug for 4 continuous days. The experiment began at 1h after the last administration. 2. Anti-asthmatic effect on drug induced asthma in guinea pigs
Animals in each group were placed into air-tight organic cover and asthma was induced by the method described above. After atomization, latency of asthmatic convulsion of guinea pigs was observed in 6min. If no asthmatic convulsion was observed, the latency was deemed as 6 min.
Statistical analyses of variance and t test were carried out by SPSS 10.0. The results showed that significant prolongation of latency of asthma induced by acecolex and histamine phosphate was observed in guinea pigs in the high and medium dosages groups of this inventive composition.
The group administered with the low dosage of the inventive composition also showed some effect on prolonging latency of asthma in guinea pigs, but there was no significant statistical difference compared to asthma model group. See table 1-1.
Table 1-1: Anti-asthmatic effect of the inventive composition on asthma induced by drug in guinea pigs (¥ 5)
Dosage # of Latency of induced asthma(s)
Group ; (mg/kg) Animals Wheeze Fall
Asthma model group — 12 24.619.0 40.8114.5
Aminophylline group 0.06g/kg 12 38.3113.0%* 74.3436.0%%
Dingchuan decoction group 8.1 g/kg 12 33.939.1%* 50.3%18.3 5.1g/kg 10 42.2411.5%% 81.3338 1**
The inventive composition groups: high, medium, and low 2.6 glkg 10 37.7£16.9% 57.14+21.9% 1.3 g/kg 10 31.6£12.5 473178
Note: Compared with Asthma model group, * p<0.05,** p<0.01.
After breathing in a liquid mixture of histamine and acecolex, asthma would be induced in normal guinea pigs. The inventive composition with high and medium dosages (5.1g/kg and 2.6 g/kg, respectively) can significant prolong the appearance time of wheezing and falling in asthmatic guinea pigs (p<0.05 and p<0.01, respectively). After sensitization by Ovalbumin (OVA), guinea pigs produce anti-OVA IgE antibody which combines with mast cells. When antigen OVA is inhaled, degranulation is caused by the combination of OVA and IgE on cell surface, and asthma attack is induced by the release of inflammatory mediators. Administration of the high and medium dosages (5.1g/kg and 2.6g/kg, respectively) of the inventive composition can significantly prolong latency of asthma in guinea pigs. In addition, This inventive composition showed better anti-asthmatic effect than that of Dingchuan decoction, 11. The effect of the inventive composition on late-phase reaction of bronchial asthma 1. Groups and drug administration of the animals: 60 healthy pure-white guinea pigs were randomly divided into 6 groups: Asthma model group, high, medium and low dosages of the inventive composition groups (5.1g/kg, 2.6 g/kg, and 1.3 g/kg, respectively), Dingchuan decoction group (8.1g/kg), and aminophyiline positive control group (intragastrically administered aminophylline at 0.06g/kg). Animals in asthma model group was administered with a solvent of same volume. Guinea pigs were intragastically administered with respective drugs for 3 days before asthma induction, and then for 4 continuous days after. 2. Anti-asthmatic effect of this inventive composition on asthma induced by OVA in guinea pigs: Guinea pigs in each group were sensitized by intramuscular injection of 0.2ml 4% OVA saline solution in the hind leg and intraperitoneal injection of 0.2ml 4% aluminum hydroxide latex.
Guinea pigs were sensitized again by the same method at the 7th day. One hour after the last injection, asthma was induced by spraying the animals with ultrasonic nebulized asthma inducing solution for 6 minutes. The asthma inducing solution was 2% OVA saline solution, and the atomization volume was not less than 4ml/min. Latency of asthmatic convulsion in guinea pigs was observed in 6 min. If no asthmatic convulsion was observed, the latency was deemed as 6 min.
Results were analyzed using variance and T test. All analyses were carried out using SPSS 10.0.
The results showed that significant prolongation of latency of induced asthma in guinea pigs sensitized by OVA was observed in groups administered with high and medium dosages of the inventive composition. The low dosage inventive medicine composition group also showed some effect of prolonging latency of induced asthma, but there was no significant statistical difference compared with Asthma model group. See table 1-2.
Tablel-2:The effect on latency of induced asthma sensitized by OVA of guinea pigs (x ts )
Grou Dosage # of Latency of induced asthmaqs) (mlkg) ~~ Animals Wheeze Fall
Asthma model group - 10 62.4124.2 104.2453.1
Aminophylline group 0.06g/kg 10 163.2489. 1%* 238.01£76.9%*
Dingchuan decoction 8.1 glkg 10 86.5430, 7* 154.5160.0*
The inventive 5.1g/kg 10 166.6+87.0%* 206.6399.2% composition groups: high, 56 gq 10 118.0462.6* 167.6:77.6% medium and low 1.3 g/kg 10 71.7426.3 110.2441.0
Note: Compared with asthma model group,* p<0.05,** p<0.01.
Experimental model of airway inflammation caused mainly by eosinophils (EOS) infiltration can be established by sensitizing guinea pigs with OVA and attacking the animals by multiple inhalation with atomized OVA. That is known as the allergic bronchial asthma guinea pig model.
This experiment showed that guinea pigs administered with high and medium dosages (5.1g/kg and 2.6 g/kg, respectively) of the inventive composition exhibited significantly prolonged latency of OVA induced asthma , and decreased number of EOS in whole blood were observed in animals from these two groups (p<0.05 and p<0.01, respectively). It suggests that high and medium dosages of the inventive composition have better effect on delayed bronchial asthma in guinea pigs than that of Dingchuan decoction.
Experiment 2: Experiment of Relieving Cough, Removing Phlegm and Anti-inflammation
I. Experimental study on the inventive composition’s effect of relieving cough 1. Experimental materials 1.1 Experimental animals 72 healthy KM mice, half males and half females, with a body mass of 18-20g, were purchased from Beijing Vital River Experimental animal Technical Co. Ltd, certification number: SCXK (B1)2002-0003. Mice were marked with 5% picric acid and fed in captivity at 5 mice/cage. Mice were fed in the Pharmacological Laboratory of Pharmaceutical Research Institute of Hebei
Medical University, in an environment of 12h light/day, temperature between 20~23°C, and relative humidity between 40-60%. Mice were fed with whole value grain feedstuffl obtained from
Experimental Animal Center of Hebel Province and unlimited water intake. Mice were initiated onto their diets and environment for 3 days. 1.2 Experimental drugs
The inventive composition: 1 gram of the dry powder corresponds to 4.5g of crude drug. The dosage of the drug for human clinical use is 22g crude drug/day, i.e., 0.37g crude drug/kg weight (standard body weight 60kg). The drug was provided by Shijiazhuang Yiling Pharmaceutical Co.,
Ltd, batch number : 20060801. The medicine was combined with 0.5%CMC — Na to prepare a suspension with required concentration before use.
Dingchuan decoction: Provided by Shijiazhuang Yiling Pharmaceutical Co., Ltd. The human clinical dosage of this medicine is 69g crude drug/day. This medicine was combined with 0.5%CMC — Na to prepare a solution with required concentration before use.
Compound Codeine Phosphate Solution: 0.9mg/ml, Hong Kong Aomei Pharmaceutical Factory, batch number: 060115. 1.3 Experimental reagent
Concentrated sulfuric acid: analytical pure, Beijing Chemical Factory, batch number: 20020809. 2. Experimental method 2.1 Principle of dosage setting
The maximum clinical dosage of the inventive composition is 22g crude drug/day, i.e. 037g crude drug/kg (standard body weight is 60kg). The dosages administered to mice were 5, 10, and times of the clinical dosage, i.e., mice were given 1.85g, 3.7g, and 7.4g crude drug/kg/day (low, medium and high dosage groups, respectively). Dingchuan decoction group was administered at 11.5g crude drug/kg/day, which is 10 times of clinical dose. Control group and positive drug groups were separately established. 2.2 Administration routes and dosages
Between 9:00-10:00 AM everyday, mice were intragastrically administered with drugs corresponding to their groups and consistent with oral administration route recommended in clinic.
Drugs were administered for 5 continuous days at 0.1ml/10g body weight.
2.3 Administration dosages and grouping
According to the above principle of dosage setting, mice were divided into the following 5 groups, see table 2-1:
Table2-1 Groups and administration dosages in the experiment of relieving mice cough with the inventive composition
Group #of Animals Dosage (g crude drug relations to human fkg.d) clinical dosage (times)
Control group 12 -- --
Codeine phosphate 12 0.03 10 solution group
Dingchuan decoction 12 11.5 10 group
The inventive 12 1.85 5 composition groups: 12 3.70 10 low, medium and high 12 7.40 20 2.4 Experimental procedures 2.4.1 Preparation of sulfur dioxide: took a 250ml flask with side port which was connected with bladder by rubber tube; the flask was loaded with hydrosulfite of sodium; fixed a buret on the
Co bottle stopper, and loaded the buret with concentrated sulfuric acid; opened the piston of buret to allow the dropping of concentrated sulfuric acid; gas sulfur dioxide was produced and the gas filled in the bladder; clamped the bladder with a haemostat; took 4-10ml of the gas with a syringe before using. 2.4.2 KM mice were randomly divided into 6 groups, 12 mice each group with half males and half females. The groups were control group, Dingchuan decoction group, the codeine phosphate group, high, medium and low dosages of the inventive composition groups. All groups were administered intragastrically with corresponding drugs at 0.1ml/10g for 5 continuous days.
Control group was administrated with 0.5%CMC-Na. 0.5 hour after the last administration, mice of each group were subsequently put into a 250ml jar injected with sulfur dioxide. Latency (the time from sulfur dioxide injection to the occurrence of coughing) and frequency of cough were observed in 2 minutes. Typical cough in mice shows abdominal contractions and wide opening of the mouth at the same time, and sometimes it is accompanied with coughing sound. Cough relieving rate was calculated using the following formula:
Cough relieving rate (%) = (cough reaction time of the administration group/cough reaction time of the control group) *100%. 3. Statistical method
Experimental data were analyzed with SPSS 11.5. Statistical results are expressed by mean numberkstandard deviation( xts }; difference between groups was performed by one-factor analysis of variance. 4. Experimental results
The effect of the inventive composition on cough induced by sulfur dioxide was observed and shown in table 2.2. The results show that the latency of cough induced in mice in the control group was 19.5848.17 seconds and cough frequency in 2 minutes was 69.25£26.93 times. Latency of cough in mice in the low, medium and high dosages of the inventive composition groups were 54.58%35.57 seconds, 57.67+30.53 seconds and 73.50237.81 seconds, respectively. The lengths of latency in these groups were remarkably prolonged compared with that of the control group (P< 0.05 or P < 0.01); cough frequencies in 2 minutes were 27.42£22.86 times, 27.08+17.17 times and 11.92+19.44 times, respectively. Cough frequencies remarkably reduced compared to that of the control group (P<<0.01).
Table 2-2 The effect of the inventive composition on cough induced by sulfur dioxide( XEs)y
Group Dose (g crude # of Weight Cough Cough drug /kg.d) Animals(n) (g) latency (5) frequency (n)
The control group -- 12 26.0+2.6 19.58+8.17 69.25+26.93
Codeine 0.03 12 25.8+1.8 48.83422 93% 23.00£18.46%*
Dingchuan 11.5 12 26.1123 54.42+39.72 28.3442(.19%*
Low dose group 1.85 12 26.842.2 54.58435.57 | 27.4222 §64*
Medium dose 3.70 12 26.3+2.4 57.67£30.53% 27.08+17.17%* group
High dose group 7.40 12 26.9+2.6 73.50£37.81%* 11.92+19.44**
Note: Compared with the control group,* P<0.05,** P<0.01.
It is observed that cough latency and cough frequency of groups administered with high and medium dosage of the inventive composition were remarkably prolonged compared to those of the
Dingchuan decoction group (P < 0.05). It suggests that this inventive composition has a significant effect on relieving cough, and its effect is better than that of Dingchuan decoction.
II Experimental study of the inventive composition’s effect on removing phlegm 1. Experimental materials 1.1 Experimental animals 72 healthy KM mice, half males and half females, with a body mass of 18-20g, were purchased from Beijing Vital River Experimental animal Technical Co. Ltd, certification number: SCXK (BJ) 2002-0003. Mice were marked with 5% picric acid and fed in captivity at 5 mice/cage. Mice were fed in Pharmacological Laboratory of Pharmaceutical Research Institute of Hebei Medical
University in a condition of 12h light/day, temperature between 20~23°C, and relative humidity between 40-60%. Mice were fed with whole value grain feedstuif obtained from Experimental
Animal Center of Hebei Province and unlimited water supply. Mice were initiated for 3 continuous days. 1.2 Experimental drugs
The inventive composition: 1 gram of the dry powder composition drug corresponds to 4.5g crude drug. The clinical dosage for adults is 22g crude drug/day, i.e., 0.37g crude drug/kg body weight (standard bodyweight is 60kg). The medicine was provided by Shijiazhuang Yiling
Pharmaceutical Co., Ltd, batch number: 20060801. The drug was combined with 0.5% CMC — Na to prepare a suspension with required concentration before use.
Dingchuan decoction: provided by Shijiazhuang Yiling Pharmaceutical Co., Ltd. Clinical dosage of the drug for human is 69g crude drug/day. The drug was combined with 0.5% CMC — Na to prepare a solution with required concentration before use.
Ammonium chloride: 30mg/tablet, obtained from Xinan Pharmaceutical Co., Ltd, batch number: 66050005. 2. Experimental methods 2.1 Principle of dosage setting
The maximum clinical dosage of this inventive composition is 22g crude drug/day, that is, 0.37g crude drug/kg (standard bodyweight is 60kg). The dosages administered to mice were 5, 10 and 20 times of the clinical dosage, that is, mice were given 1.85g, 3.7g and 7.4g crude drug/kg/day (low, medium and high dosage group, respectively); Dingchuan decoction group: the drug was administrated at 11.5g crude drug/kg/day, that is, 10 times of clinical dosage. The control group and the positive drug group were separately established. 2.2 Administration routes and dosages
Between 9:00-10:00 AM everyday, mice were intragastrically administered with medicine corresponding to their group and consistent with oral administration route recommended in clinic.
Dosage was set at 0.1ml/10g bodyweight for 5 continuous days. 2.3 Administration dosages and groups
According to the principle of dosage setting, mice were divided into the following 6 groups, see table 2-3:
Table2-3 Groups and administration dosages in experiment of relieving mice cough by the inventive composition
Group # of Animals Dose (g crude Relations to human drug /kg.d) clinical dosage (times)
The control group 12 -- --
The ammonium 12 1.00 10 chloride group
Dingchuan 12 11.5 10 decoction group
The inventive 12 1.85 5 : composition groups: low,
Medium 12 3.70 10
High 12 7.40 20 2.4 Experimental procedures
KM mice were divided randomly into 6 groups, 12 mice in each group with half males and half females. The groups were: the control group, the ammonium chloride, Dingchuan decoction group, low, medium and high dosages of the inventive composition groups. All groups were administrated with corresponding medicine intragastrically at 0.1ml/10g for 5 continuous days.
The control group was given 0.5% CMC-Na. 0.5 hour after the last administration, mice of each group (after fasting for 16h) were injected with phenol red 0.lml(5mg)/10g weight by intraperitoneal injection. At 0.5 hour after injection, mice were killed. Tissues around organs were peeled to expose the trachea and cut the trachea located between thyroid cartilage and tracheal branch; put the trachea into the test tube with 2ml saline followed by adding 0.1ml (or 1-2 drops) sodium hydroxide, then mixed well; OD values were measured by enzyme-labeling measuring instrument 540nm, and phenol red was used to establish standard curve and to calculate the content of phenol red according to the curve{pg/ml), and compared that with the control group. 2.5 Statistical method
Experimental data were analyzed using SPSS 11.5. Statistical results are expressed by mean number#standard deviation(* ts }. Difference between groups was performed by one-factor analysis of variance. 3. Experimental results
The phlegm removing effect of this inventive composition was observed using the trachea phenol red method. The results, which are shown in table 2.4, indicate that phenol red content in the trachea of mice in the control group was 0.43£0.12 ug/ml, phenol red contents in trachea of mice in the low, medium and high dosages of the inventive composition groups were 0.82%0.34 ug/ml, 1.231£0.31 ug/ml and 1.48+0.61 ug/ml, respectively.
Table 2-4 Results of phlegm removing effect of the inventive medicine composition (% ts )
Group Dose (g crude Relations to # of Phenol red content drug/kg.d human clinical Animals ged) ] in trachea(ug/ml) dosage (times)
Control -- -- 12 0.43+0.12 group
Ammonium 1.00 10 12 ) 1.4140.41%%* chloride group
Dingchuan 11.5 10 12 0.89%0.37** decoction group
Low dosage 1.85 5 12 0.82£0.34%* group
Medium 3.70 10 12 1.2320.31%* dosage group
High dosage 7.40 20 12 1.4820.61%* group
Note: Compare with the model group: *P < 0.05,%*P < 0.01.
Remarkable increases in phenol contents were observed in the inventive composition groups, compared to those in the model group and Dingchuan decoction group (P<<0.01 or P<0.05). It suggests that this inventive composition has a significant effect on removing phlegm, and its effect is better than that of Dingchuan decoction.
Ill Anti-general inflammation effect of this inventive composition 1 Experimental materials 1.1 Animals : SD rats, half males and half females, with a body weight of 160-180g, were purchased from Beijing Vital River Experimental animal Technical Co. Ltd, certification number:
SCXK (BI)2002-0003. 1.2 Drugs and reagents (1) Testing drugs: 1g of the dry powder inventive composition corresponds to 4.5g crude drug.
The clinical dose for adult is 22g crude drug/day, that is, 0.37g crude drug/kg weight (standard weight is 60kg). The medicine was provided by Shijiazhuang Yiling Pharmaceutical Co., Ltd, batch number: 20060801. The medicine was combined with 0.5%CMC — Na to prepare a suspension with required concentration before use. (2) Positive control drug: Dexamethasone tablets were produced by Tianjin Tianyao
Pharmaceutical Co., Ltd, batch number: 20060711. License number: guo yao zhun zi H12020686.
The drug was combined with 0.5% CMC before use. (3) Carrageenin: Product of Sigma Company. At one day before use, the drug was pulverized into fine powder, combined with saline for injection to prepare 1% uniform suspension, and stored in 4°C refrigerator. 1.3 Apparatus: Instrument for determining rats' foot volume (drainage). 2. Methods and results 2.1 Groups and drug administration: 60 rats for experiment were randomly divided into 6 groups: the model group (administered intragastrically the same volume of 0.5%CMC),
Dingchuan decoction group (administered intragastrically 8.7g/kg , equal to 7.5 times of clinical dose for human), the positive drug (administered intragastrically dexamethasone 0.1g/kg , equal to 7.5 times of clinical dose for human), high, medium and low doses of this inventive composition (administrated intragastrically of 5.6g/kg, 2.8g/kg and 1.4g/kg , respectively; equal to 15, 7.5, 3.75 times of clinical doses for human, respectively); each group had 10 rats. Drugs were administered for 3 continuous days, and models were established at 60 minutes after last administration. 2.2 Voix Pedis Swelling Method: according to the reference [Chen, Qi. chief editor. Study
Methodology of Pharmacology of Traditional Chinese Medicine, People’s Health Publishing
House, 1993, 364p], the volume of each rat’s left post voix pedis was determined and recorded before drug administration; 60 minutes after last administration, subcutaneously injected 0. lml/rat 1% carrageenin suspension in each rat’s left post voix pedis, then measured and recorded the left post voix pedis volume every hour, and measured 6 times continuously. Recorded the results and calculated the swelling degree and the inhibition rate according to the following formula: swelling rate (%) = (volume of voix pedis after induced inflammation - volume of voix pedis before induced inflammation) / volume of voix pedis before induced inflammation* 100% inhibition rate (%) = (average swelling degree of the control group - average swelling degree of the administration group) / average swelling degree of the control group 100%
Statistical analyses of variance and group T test were carried out using SPSS 10.0 2.3 Results of the anti-general inflammation effect of the inventive composition are shown in table 2-5. Voix pedis swelling in rats induced by carrageenin injection was significantly inhibited by injecting the inventive composition with the doses of 5.6g /kg and 2.8g /kg. At 1h after model had been established, the inhibition rates were significantly different from that of the control group, and the inhibitive effect lasted for 6h (p<0.05 and p<Q.01, respectively). The results showed that high and medium doses of this inventive composition significantly inhibited voix pedis swelling induced by injecting carrageenin in rats. 5.6g /kg and 2.8g /kg of this inventive composition also significantly inhibited the formation of granuloma of rat’s tampon, compared with that of the control group (p<0.01 and p<0.05, respectively). These effects of the inventive composition were all better than that of Dingchuan decoction group.
Table 2-3. The effect of the inventive medicine composition on voix pedis swelling induced by carrageenin in rats
Dose # of Swelling degree (%) ne ew mm
The model - 10 47.6:13.4 67.2+16.3 0415284 120.8+31.1 117.9424.7 110.6+£30.1 group
Dexamethaso 0.1 10 29.1+16.4* 31.4+15.2 34.6:13.8" 42.0+15.7* 45.5%+19.3* 54. 7+25.2*
ne group mgikg (38.85%) (63.22%) (63.20%) (65.25%) (61.35%) (50.53%)
Dingchuan . 45.3+16.5 51.9+26.3 66.9+21.8* 89.8+27.8" 93.2423.7" 110.1£25.2 decoction 8.7 10 (4.83%) (22.77%) (28.91%) (25.67%) (20.95%) (0.45%) group
High medium 44.1184 49.3+14.9% 6574235 76.3+33.57 83.4z221% 103.2+30.1 5.6 10 and low doses (7.38%) (26.61%) (30.15%) (36.85%) (29.23%) (6.75%) of the 41.6:17.6 50.2+13.4* 66.7+23.1* 77.7£24.8"( 93.3225.3* 106.5+£24.3 2.8 10 inventive (12.58%) (25.38%) (29.15%) 35.63%) (20.88%) (3.77%) composition 0 42.3:16.9 52.0+25.7 68.6:27.6 92.8£33.1* 95.3+31.4 110.8£23.9 1.4 1 groups (11.16%) (27.76%) (27.09%) (26.03%) (19.10%) (-0.17%)
Note: Compared with the model group, *p<0.05, **p<0.01. (The numbers in bracket are inhibition rate of swelling) 3. Conclusion: This inventive composition has significant inhibitive effect on general inflammation, and the effect is better than that of Dingchuan decoction,
Bronchial asthma is a chronic inflammation disease caused by multiple cells and cellular components. It can cause airway hyperreactivity which induces wheezing, dyspnea, chest distress and repeated attacks of coughing. The etiology is related to atopic allergy. At the same time, the etiology of some patients is undetermined. Symptomatic treatment and eticlogical treatment are both adopted. Because herbal compounds have multi-link comprehensive effects, they have unique advantages in treating bronchial asthma. Therefore, the pharmacodynamic studies of this inventive composition were based on its effect of dispersing lung-gi, relieving asthma, clearing heat and removing phlegm, and focus on improveming the symptoms by removing phlegm, relieving cough and anti-inflammation. At the same time, these studies observed the inventive composition’s effects on related mechanisms such as allergy reactions, mast cells degranulation, immunoregulation and so on to comprehensively evaluate the medicine’s therapeutic effects and mechanisms. :
The pharmacodynamic study results suggest that high and medium doses of the inventive composition can both significantly prolong the time of appearance of wheezing and falling in guinea pigs with asthma induced by mixed solution of histamine and acetylcholine chloride, and also can significantly prolong the latency in guinea pigs with asthma induced by OVA. For late-phase reaction asthma induced by multiple attacks of OVA, high and medium doses of this inventive composition can significantly prolong the latency of last asthma induced by OVA,
decrease the proportions of eosinophils in whole blood and in bronchoalveolar lavage fluid. In addition, high and medium doses of this inventive composition can inhibit Leukotriene B4 production in bronchoalveolar lavage fluid. Histopathology of bronchoalveolar also suggests that high, medium and low doses of this inventive composition can all decrease the infiltration number of eosinophils in bronchial wall and improve pathological injuries caused by inflammation in bronchopulmonary tissue. These results suggest that this inventive composition has significant treatment effects on both late-phase reaction and early-phase reaction of bronchial asthma, and has significant inhibitive effects on the production of relevant inflammatory cells and mediators.
Animal experiments of relieving cough, removing phlegm, antiinflammation and so on were adopted to observe clinical related symptoms and the symptom improvement effect of this inventive composition. The results suggest that high, medium and low doses of this inventive composition significantly prolonged the cough latent period in mice induced by sulfur dioxide and reduced the frequencies of coughing in mice. Trachea phenol red experiment results suggest that high, medium and low doses of this inventive composition significantly increased secretion of phenol red of mice trachea. Anti-inflammation experiment results suggest that high and medium doses of this inventive composition significantly inhibited voix pedis swelling in rats induced by injecting carrageenin and the formation of granuloma of rat’s tampon. At lh after model establishment, compared to control group, significant difference was found in the inventive composition groups, and the difference lasted for 6h. The above results suggest that this inventive composition has significant effects on relieving cough, removing phlegm and anti-inflammation, and has a good assistant effect on the improvement of asthma symptoms.
The preparation of the present inventive traditional Chinese medicine composition is illustrated through the following examples but not limited to the range of the present invention.
Example 1
The preparation of tablets of the inventive composition (temporarily named: LIAN HUA asthma relieving tablets);
Formulation:
Herba Ephedrae (honey-baking) 63g, Cortex Mori 210g, Lumbricus 210g,
Semen ginkgo 84g, Flos Farfarae 84g, Rhizoma Pinelliae (alum processed) 84g,
Fructus Perillae (stir-fried) 84g, Radix Paeoniae Rubra 210 g,
Semen Armeniacae Amarum (stir-fried) 84g, Radix Scutellariae 84g,
Periostracum Cicadae 84g, Herba Houttuyniae 210g,
Radix Trichosanthis 210g, and Fructus Forsythiae 84g.
Preparation method: a) taking 84g of Radix Scutellariae and 42g of Rhizoma Pinelliae, pulverizing them into fine powder for later use; b) taking the relative amounts of Herba Ephedrae, Semen Ginkgo, Cortex Mori, Flos Farfarae,
Fructus Perillae, Semen Armeniacae Amarum and Herba Houttuyniae according to the formulation and the rest of Rhizoma Pinelliae, adding 8 times amount of 60% ethancl, heating and refluxing extract thrice, 1.5 hours each time, recovering ethanol under reduced pressure from extract solution, concentrating the solution to obtain the extract with a relative density of 1.08 at 60°C for later use; ¢) taking the relative amounts of Lumbricus, Radix Paeoniae Rubra, Periostracum Cicadae,
Radix Trichosanthis and Fructus Forsythiae according to the formulation, adding 9 times amount of water, decocting twice, 2 hours each time, filtering the water decoction, combining decoctions, concentrating the solution to obtain the extract with a relative density of 1.08 at 60°C, and combining the extract with extract of step b) for later use; d) Mixing the fine powder of step a} and the extract of step ¢), adding an appropriate amount of adjuvant into the mixture, and spraying granulation to obtain granules and sifting the granules through a mesh after drying, standing by. ; and e) preparing the granules of step d) to obtain tablets of the inventive composition through routine processes.
Example 2
The preparation of capsules of the inventive composition:
Formulation:
Herba Ephedrae 61g, Cortex Mori 198g, Lumbricus 223g,
Semen ginkgo 78g, Flos Farfarae 92g, Rhizoma Pinelliae 73g,
Fructus Perillae 85g, Radix Paeoniae Rubra 188g, Semen Armeniacae Amarum 87g,
Radix Scutellariae 88g, Periostracum Cicadae 79g, Herba Houttuyniae 221g,
Radix Trichosanthis 218g, and Fructus Forsythiae 81 g.
Preparation method: a) taking 88g of Radix Scutellariae and 36.5g of Rhizoma Pinelliae, pulverizing them into fine powder for later use; b) taking the relative amounts of Herba Ephedrae, Semen Ginkgo, Cortex Mori, Flos Farfarae,
Fructus Perillae, Semen Armeniacae Amarum and Herba Houttuyniae according to the formulation, and the rest of Rhizoma Pinelliae adding 6 times amount of 50% ethanol, heating and refluxing extract thrice, 1 hour each time, recovering ethanol under reduced pressure from extract solution, concentrating the solution to obtain the extract with a relative density of 1.05 at 60°C for later use; ¢) taking the relative amounts of Lumbricus, Radix Paeconiae Rubra, Periostracum Cicadae,
Radix Trichosanthis and Fructus Forsythiae according to the formulation, adding 7 times amount of water, decocting twice, 1 hour each time, filtering the water decoction, combining decoctions, concentrating the solution to obtain the extract with a relative density of 1.05 at 60°C, and combining the extract with extract of step b) for later use; d) Mixing the fine powder of step a) and the extract of step c), adding an appropriate amount of adjuvant into the mixture, and spraying granulation to obtain granules and sifting the granules through a mesh after drying, standing by. ; and ¢) preparing the granules of step d) to obtain capsules of the inventive composition through routine processes.
Example 3
The preparation of powder of the inventive composition:
Formulation:
Herba Ephedrae 50g, Cortex Mori 253g, Lumbricus 166g,
Semen ginkgo 66g, Flos Farfarae 101g, Rhizoma Pinelliae 101g,
Fructus Perillae 66g, Radix Paconiae Rubra 166g, Semen Armeniacae Amarum 101g,
Radix Scutellariae 66g, Periostracum Cicadae 66g, Herba Houttuyniae 253g,
Radix Trichosanthis 253g, and Fructus Forsythiae 66 g.
Preparation method: a} taking Radix Scutellariae and half amount of Rhizoma Pinelliae according to the formulation proportion, pulverizing them into fine powder for later use; b) taking the relative amounts of Herba Ephedrae, Semen Ginkgo, Cortex Mori, Flos Farfarae,
Fructus Perillae, Semen Armeniacae Amarum and Herba Houttuyniae according to the formulation and the rest of Rhizoma Pinelliae, adding 10 times amount of 80% ethanol, heating and refluxing extract thrice, 3 hours each time, recovering ethanol under reduced pressure from extract solution, concentrating the solution to obtain the extract with a relative density of 1.10 at 60°C for later use; c) taking the relative amounts of Lumbricus, Radix Paeoniae Rubra, Periostracum Cicadae,
Radix Trichosanthis and Fructus Forsythiae according to the formulation, adding 10 times amount of water, decocting twice, 4 hours each time, filtering the water decoction, combining decoctions, concentrating the solution to obtain the extract with a relative density of 1.10 at 60°C, and combining the extract with extract of step b) for later use; and d)making the fine powder of step a) and combined extract of step ¢) into powder through routine processes.
Example 4
The preparation of oral solution of the inventive composition:
Formulation:
Herba Ephedrae 76g, Cortex Mori 166g, Lumbricus 253g,
Semen ginkgo 101g, Flos Farfarae 66g, Rhizoma Pinelliae 66g,
Fructus Perillae 101g, Radix Paconiae Rubra 253g, Semen Armeniacac Amarum 66g,
Radix Scutellariae 101g, Periostracum Cicadae 101g, Herba Houttuyniae 166g,
Radix Trichosanthis 166g, and Fructus Forsythiae 101 g.
Preparation method: a) taking Radix Scutellariae and half amount of Rhizoma Pinelliae according to the formulation proportion, pulverizing them into fine powder;
b) taking the relative amounts of Herba Ephedrae, Semen Ginkgo, Cortex Mori, Flos Farfarae,
Fructus Perillae, Semen Armeniacae Amarum and Herba Houttuyniae according to the formulation and the rest of Rhizoma Pinelliae, adding 6 times amount of 80% ethanol, heating and refluxing extract thrice, I hour each time, recovering ethanol under reduced pressure from extract solution, concentrating the solution to obtain the extract with a relative density of 1.05-1.10 at 60°C for late ruse; c) taking the relative amount of Lumbricus, Radix Paconiae Rubra, Periostracum Cicadae,
Radix Trichosanthis and Fructus Forsythiae according to the formulation, adding 7 times amount of water, decocting twice, 1 hour each time, filtering the water decoction, combining decoctions, concentrating the solution to obtain the extract with a relative density of 1.05-1.10 at 60°C, and combining the extract with extract of step b) for later use; and d) making the fine powder of step a) and combined extract of step ¢) into oral solution through routine processes.
Example 5
The preparation of soft capsules of the inventive composition:
Formulation:
Herba Ephedrae 70g, Cortex Mori 241g, Lumbricus 245g,
Semen ginkgo 98g, Flos Farfarae 94g, Rhizoma Pinelliae 95g,
Fructus Perillae 94g, Radix Paconiae Rubra 230g, Semen Armeniacae Amarum 92g,
Radix Scutellariae 91g, Periostracum Cicadae 97g, Herba Houttuyniae 237g,
Radix Trichosanthis 231g, and Fructus Forsythiae 91 g.
Preparation method: a) taking Radix Scutellariae and 1/3 amount of Rhizoma Pinelliae according to the formulation proportion, pulverizing them into fine powder for later use; b) taking the relative amounts of Herba Ephedrae, Semen Ginkgo, Cortex Mori, Flos Farfarae,
Fructus Perillae, Semen Armeniacae Amarum and Herba Houttuyniae according to the formulation and the rest of Rhizoma Pinelliae, adding 10 times amount of 50% ethanol, heating and refluxing extract thrice, 3 hours each time, recovering ethanol under reduced pressure from extract solution, concentrating the solution to obtain the extract with a relative density of 1.05-1.10 at 60°C for later use;
¢) taking the relative amounts of Lumbricus, Radix Paecniae Rubra, Periostracum Cicadae,
Radix Trichosanthis and Frucius Forsythiae according to the formulation, adding 10 times amount of water, decocting twice, 4 hours each time, filtering the water decoction, combining decoctions, concentrating the solution to obtain the extract with a relative density of 1.05-1.10 at 60°C, and combining the extract with extract of step b} for later use; and d) making the fine powder of step a) and the combined extract of step c) into soft capsules through routine processes.
Example 6
The preparation of pills of the inventive composition:
Formulation:
Herba Ephedrae 76g, Cortex Mori 253g, Lumbricus 253g,
Semen ginkgo 101g, Flos Farfarae 101g, Rhizoma Pinelliae 101g,
Fructus Perillae 101g, Radix Paconiae Rubra 253g, Semen Armeniacae Amarum 101g,
Radix Scutellariae 101g, Periostracum Cicadae 101g, Herba Houttuyniae 253g,
Radix Trichosanthis 253g, and Fructus Forsythiae 101 g.
Preparation method: a) taking Radix Scutellariae and 2/3 amount of Rhizoma Pinelliae according to the formulation proportion, pulverizing them into fine powder for later use; b) taking the relative amounts of Herba Ephedrae, Semen Ginkgo, Cortex Mori, Flos Farfarae,
Fructus Perillae, Semen Armeniacae Amarum and Herba Houttuyniae according io the formulation and the rest of Rhizoma Pinelliae, adding 8 times amount of 60% ethanol, heating and refluxing extract thrice, 1.5 hours each time, recovering ethanol under reduced pressure from extract solution, concentrating the solution to obtain the extract with a relative density of 1.05-1.10 at 60
C for later use; c) taking the relative amounts of Lumbricus, Radix Paeoniae Rubra, Periostracum Cicadae,
Radix Trichosanthis and Fructus Forsythiae according to the formulation, adding 8 times amount of water, decocting twice, 3 hours each time, filtering the water decoction, combining decoctions, concentrating the solution to obtain the extract with a relative density of 1.05-1.10 at 60°C, and combining the extract with extract of step b) for later use; and d) making fine powder of step a) and combined extract of step ¢) into pills through routine processes.
Example 7
The preparation of tincture of the inventive composition:
Formulaticn:
Herba Ephedrae 50g, Cortex Mori 166g, Lumbricus 166g,
Semen ginkgo 66g, Flos Farfarae 66g, Rhizoma Pinelliae 66g,
Fructus Perillac 66g, Radix Paeoniae Rubra 166g, Semen Armeniacae Amarum 66g,
Radix Scutellariae 66g, Periostracum Cicadae 66g, Herba Houttuyniae 166g,
Radix Trichosanthis 166g, and Fructus Forsythiae 66 g.
Preparation method: a) taking Radix Scutellariae and half amount of Rhizoma Pinelliae according to the formulation proportion, pulverizing them into fine powder for later use; b) taking the relative amounts of Herba Ephedrae, Semen Ginkgo, Cortex Mori, Flos Farfarae,
Fructus Perillae, Semen Armeniacae Amarum and Herba Houttuyniae according to the formulation and the rest of Rhizoma Pinelliae, adding 9 times amount of 75% ethanol, heating and refluxing extract thrice, 2.5 hours each time, recovering ethanol under reduced pressure from extract solution, concentrating the solution to obtain the extract with a relative density of 1.05-1.10 at 60 ‘C for later use; c) taking the relative amounts of Lumbricus, Radix Paeoniae Rubra, Periostracum Cicadae,
Radix Trichosanthis and Fructus Forsythiae according to the formulation, adding 8.5 times amount of water, decocting twice, 2.5 hours each time, filtering the water decoction, combining decoctions, concentrating the solution to obtain the extract with a relative density of 1.05-1.10 at 60°C, and combining the extract with extract of step b) for later use; and d) making fine powder of step a} and combined extract of step ¢) into tincture composition through routine processes.
Example 8
The preparation of syrup of the inventive composition:
Formulation:
Herba Ephedrae 50g, Cortex Mori 166g, Lumbricus 166g,
Semen ginkgo 66g, Flos Farfarae 66g, Rhizoma Pinelliae 66g,
Fructus Perillae 66g, Radix Paconiae Rubra 166g, Semen Armeniacae Amarum 66g,
Radix Scutellariae 66g, Periostracum Cicadae 66g, Herba Houttuyniae 166g,
Radix Trichosanthis 166g, and Fructus Forsythiae 66 g.
Preparation method: a) taking Radix Scutellariae and half amount of Rhizema Pinelliae according to the formulation proportion, pulverizing them into fine powder for later use; b) taking the relative amounts of Herba Ephedrae, Semen Ginkgo, Cortex Mori, Flos Farfarae,
Fructus Perillae, Semen Armeniacae Amarum and Herba Houttuyniae according to the formulation and the rest of Rhizoma Pinelliae, adding 7.5 times amount of 70% ethanol, heating and refluxing extract thrice, 1.5 hours each time, recovering ethanol under reduced pressure from extract solution, concentrating the solution to obtain the extract with a relative density of 1.05-1.10 at 60°C for later use; ¢) taking the relative amounts of Lumbricus, Radix Paconiae Rubra, Periostracum Cicadae,
Radix Trichosanthis and Fructus Forsythiae according to the formulation, adding 8 times amount of water, decocting twice, 2 hours each time, filtering the water decoction, combining decoctions, concentrating the solution to obtain the extract with a relative density of 1.05-1.10 at 60°C, and combining the extract with extract of step b) for later use; and d) making fine powder of step a) and combined extract of step ¢) into syrup through routine processes.
Example 9
The preparation of suppository of the inventive composition:
Formulation:
Herba Ephedrae 50g, Cortex Mori 253g, Lumbricus 166g,
Semen ginkgo 66g, Flos Farfarae 101g, Rhizoma Pinelliae 101g,
Fructus Perillac 66g, Radix Paeoniae Rubra 166g, Semen Armeniacae Amarum 101g,
Radix Scutellariae 66g, Periostracum Cicadae 66g, Herba Houttuyniae 253g,
Radix Trichosanthis 253g, and Fructus Forsythiae 66 g.
Preparation method: a) taking Radix Scutellariae and 2/3 amount of Rhizoma Pinelliae according to the formulation proportion, pulverizing them into fine powder for later use;
b) taking the relative amounts of Herba Ephedrae, Semen Ginkgo, Cortex Mori, Flos Farfarae,
Fructus Perillae, Semen Armeniacae Amarum and Herba Houttuyniae according to the formulation and the rest of Rhizoma Pinelliae, adding 6 times amount of 50% ethanol, heating and refluxing extract thrice, 3 hours each time, recovering ethane] under reduced pressure from extract solution, concentrating the solution to obtain the extract with a relative density of 1.05-1.10 at 60°C for later use; c) taking the relative amounts of Lumbricus, Radix Paeoniae Rubra, Periostracum Cicadae,
Radix Trichosanthis and Fructus Forsythiae according to the formulation, adding 7 times amount of water, decocting twice, 1 hour each time, filtering the water decoction, combining decoctions, concentrating the solution to obtain the extract with a relative density of 1.05-1.10 at 60°C, and combining the extract with extract of step b) for later use; and d) makeing fine powder of step a) and combined extract of step ¢) into suppository through routine processes.
Example 10
The preparation of gelata of the inventive composition:
Formulation:
Herba Ephedrae 76g, Cortex Mori 166g, Lumbricus 253g,
Semen ginkgo 101g, Flos Farfarae 66g, Rhizoma Pinelliae 66g,
Fructus Perillae 101g, Radix Paconiae Rubra 253g, Semen Armeniacae Amarum 66g,
Radix Scutellariae 101g, Periostracum Cicadae 101g, Herba Houttuyniae 166g,
Radix Trichosanthis 166g, and Fructus Forsythiae 101 g.
Preparation method: a) taking Radix Scutellariae and 3/4of Rhizoma Pinelliae according to the formulation proportion, pulverizing them into fine powder for later use; b) taking the relative amounts of Herba Ephedrae, Semen Ginkgo, Cortex Mori, Flos Farfarae,
Fructus Perillae, Semen Armeniacae Amarum and Herba Houttuyniae according to the formulation and the rest of Rhizoma Pinelliae, adding 10 times amount of 50% ethanol, heating and refluxing extract thrice, 3 hours each time, recovering ethanol under reduced pressure from extract solution, : concentrating the solution to obtain the extract with a relative density of 1.05-1.10 at 60°C for later use;
¢) taking the relative amounts of Lumbricus, Radix Paeoniae Rubra, Periostracum Cicadae,
Radix Trichosanthis and Fructus Forsythiae according to the formulation, adding 10 times amount of water, decocting twice, 4 hours each time, filtering the water decoction, combining decoctions, concentrating the solution to obtain the extract with a relative density of 1.05-1.10 at 60°C, and combining the extract with extract of step b) for later use; and d) making fine powder of step a) and combined extract of step ¢} into gelata through routine
Processes.
Example 11
The preparation of spray of the inventive composition:
Formulation:
Herba Ephedrae 70g, Cortex Mori 241g, Lumbricus 245g,
Semen ginkgo 98g, Flos Farfarae 94g, Rhizoma Pinelliae 95g,
Fructus Perillae 94g, Radix Paeoniae Rubra 230g, Semen Armeniacae Amarum 92g,
Radix Scutellariae 91g, Periostracum Cicadae 97g, Herba Houttuyniae 237g,
Radix Trichosanthis 231g, and Fructus Forsythiae 91 g.
Preparation method: a) taking Radix Scutellariae and half amount of Rhizoma Pinelliae according to the formulation proportion, pulverizing them into fine powder for later use; b) taking the relative amounts of Herba Ephedrae, Semen Ginkgo, Cortex Mori, Flos Farfarae,
Fructus Perillae, Semen Armeniacae Amarum and Herba Houttuyniae according to the formulation and the rest of Rhizoma Pinelliae, adding 6 times amount of 80% ethanol, heating and refluxing extract thrice, 3 hours each time, recovering ethanol under reduced pressure from extract solution, concentrating the solution to obtain the extract with a relative density of 1.05-1.10 at 60°C for later use; ¢) taking the relative amounts of Lumbricus, Radix Paeoniae Rubra, Periostracum Cicadae,
Radix Trichosanthis and Fructus Forsythiae according to the formulation, adding 10 times amount of water, decocting twice, 1 hour each time, filtering the water decoction, combining decoctions, concentrating the solution to obtain the extract with a relative density of 1.05-1.10 at 60°C, and combining the extract with extract of step b) for later use; and d) making fine powder of step a) and combined extract of step ¢} into spray through routine processes.
Example 12
The preparation of powder of the inventive composition:
Herba Ephedrae 82g, Cortex Mori 150g, Lumbricus 270g,
Semen ginkgo 50g, Flos Farfarae 130g, Rhizoma Pinelliae 55g,
Fructus Perillae 130g, Radix Paconiac Rubra 155g, Semen Armeniacae Amarum 55g,
Radix Scutellariae 130g, Periostracum Cicadae 55g, Herba Houttuyniae 280g,
Radix Trichosanthis 280g, and Fructus Forsythiae 130 g.
Preparation method:
Taking the formulation amounts of each raw medicinal material, pulverizing them into powder, sieving the powder ,and then making them into powder.
Claims (11)
1. A traditional Chinese medicine composition for treating Bronchial Asthma, wherein said composition is prepared from the following raw medicinal materials formulation: Herba Ephedrae 40-90 parts, Cortex Mori 136-300 parts, Lumbricus 146-300 parts, Semen Ginkgo 46-151 parts, Flos Farfarae 46-151 parts, Rhizoma Pinelliae 46-151 parts, Fructus Perillae 46-151 parts, Radix Paeoniae Rubra 146-300 parts, Semen Armeniacae Amarum 46-151 parts, Radix Scutellariae 46-151 parts, Periostracum Cicadae 46-151 parts, Herba Houttuyniae 146-300 parts, Radix Trichosanthis 146-300 parts, and Fructus Forsythiae 46-151 parts.
2. The traditional Chinese medicine composition according to Claim 1, wherein said composition is prepared from the following raw medicinal materials formulation: Herba Ephedrae 50-76 parts, Cortex Mori 166-253 parts, Lumbricus 166-253 parts, Semen Ginkgo 66-101 parts, Flos Farfarae 66-101 parts, Rhizoma Pinelliae 66-101 parts, Fructus Perillae 66-101 parts, Radix Paeoniae Rubra 166-253 parts, Semen Armeniacae Amarum 66-101 parts, Radix Scutellariae 66-101 parts, Periostracum Cicadae 66-101 parts, Herba Houttuyniae 166-253 parts, Radix Trichosanthis 166-253 parts, and Fructus Forsythiae66-101 parts.
3. The traditional Chinese medicine composition according to Claim 2, wherein said composition is prepared from the following raw medicinal materials formulation: Herba Ephedrae 70 parts, Cortex Mori 241 parts, Lumbricus 245 parts, Semen Ginkgo 98 parts, Flos Farfarae 94 parts, Rhizoma Pinelliae 95 parts, Fructus Perillae 94 parts, Radix Paeoniae Rubra 230 parts, Semen Anmeniacae Amarum 92 parts, Radix Scutellariae 91 parts, Periostracum Cicadae 97 parts, Herba Houttuyniae 237 parts, Radix Trichosanthis 231 parts, and Fructus Forsythiae 91parts.
4. The traditional Chinese medicine composition according to Claim 2, wherein said composition is prepared from the following raw medicinal materials formulation: Herba Ephedrae 63 parts, Cortex Mori 210 parts, Lumbricus 210 parts, Semen Ginkgo 84 parts, Flos Farfarae 84 parts, Rhizoma Pinelliae 84 parts,
Fructus Perillae 84 parts, Radix Paeoniae Rubra 210 parts, Semen Armeniacae Amarum 84 parts, Radix Scutellariae 84 parts, Periostracum Cicadae 84 parts, Herba Houttuyniae 210 parts, Radix Trichosanthis 210 parts, and Fructus Forsythiae 84 parts.
5. The traditional Chinese medicine composition according to Claim 2, wherein said composition is prepared from the following raw medicinal materials formulation: Herba Ephedrae 61 parts, Cortex Mori 198 parts, Lumbricus 223 parts, Semen Ginkgo 78 parts, Flos Farfarae 92 parts, Rhizoma Pinelliae 73 parts, Fructus Perillae 85 parts, Radix Paconiae Rubra 188 parts, Semen Armeniacae Amarum 87 parts, Radix Scutellariae 88 parts, Periostracum Cicadae 79 parts, Herba Houttuyniae 221 parts, Radix Trichosanthis 218 parts, and Fructus Forsythiae 81 parts.
6. The traditional Chinese medicine composition according to Claim 1, wherein said composition is prepared from the following raw medicinal materials formulation: Herba Ephedrae 77-88 parts, Cortex Mori 136-165 parts, Lumbricus 255-300 parts, Semen Ginkgo 46-65 parts, Flos Farfarae 110-151 parts, Rhizoma Pinelliae 46-65 parts, Fructus Perillae 105-151 parts, Radix Paeoniae Rubra 146-165 parts, Semen Armeniacae Amarum 46-65parts, Radix Scutellariae 103-151 parts, Periostracum Cicadae 46-63 parts, Herba Houttuyniae 255-300 parts, Radix Trichosanthis 255-300 parts, and Fructus Forsythiae 103-151 parts.
7. The traditional Chinese medicine composition according to any of claim 1-6, wherein Herba Ephedrae is honey-baking Herba Ephedrae, Semen Gingko is stir-fried Semen Gingko, Rhizoma Pinelliae is alum processed Rhizoma Pinelliae, Fructus Perillae is stir-fried Fructus Perillae, Semen Armeniacae Amarum is stir-fried Semen Armeniacae Amarum.
8. The traditional Chinese medicine composition according to any of claim 1-6, wherein clinical dosage forms of said composition are capsule, tablet, powder, oral solution, soft capsule, pill, tincture, syrup, suppository, gel, spray or injection.
9. The traditional Chinese medicine composition according to any of claim 1-6, wherein said composition is prepared by following method: a) taking Radix Scutellariae and 1/3—3/4 amount of Rhizoma Pinelliae according to the formulation proportion, pulverizing them into fine powder for later use; b) taking the relative amounts of Herba Ephedrae, Semen Ginkgo, Cortex Mori, Flos Farfarae, Fructus Perillae, Semen Armeniacae Amarum and Herba Houttuyniae according to the formulation and the rest of Rhizoma Pinelliae, adding 6-10 times amount of 50-80% ethanol, heating and refluxing extract thrice, 1-3 hours each time, recovering ethanol under reduced pressure from extract solution, concentrating the solution to obtain the extract with a relative density of
1.05-1.10 at 60°C for later use; c) taking the relative amounts of Lumbricus, Radix Paeoniae Rubra, Periostracum Cicadae, Radix Trichosanthis and Fructus Forsythiae according to the formulation, adding 7-10 times amount of water, decocting twice, 1-4 hour each time, filtering the water decoction, combining decoctions, concentrating the solution to obtain the extract with a relative density of 1.05-1.10 at 60°C, and combining the extract with extract of step b) for later use; and d) mixing the fine powder of step a) and the combined extract of step ¢) to obtain the inventive traditional Chinese medicine composition.
10. A preparation method of tablet of the traditional Chinese medicine composition according to any one of claim 1-6, comprising the following steps: a) taking Radix Scutellariae and half amount of Rhizoma Pinelliae according to the formulation proportion, pulverizing them into fine powder for later use; b) taking the relative amounts of Herba Ephedrae, Semen Ginkgo, Cortex Mori, Flos Farfarae, Fructus Perillae, Semen Armeniacae Amarum and Herba Houttuyniae according to the formulation and the rest of Rhizoma Pinelliae, adding 6-10 times amount of 50-80% ethanol, heating and refluxing extract thrice, 1-3 hours each time, recovering ethanol under reduced pressure from extract solution, concentrating the solution to obtain the extract with a relative density of
1.05-1.10 at 60°C for later use; c) taking the relative amounts of Lumbricus, Radix Paconiae Rubra, Periostracum Cicadae, Radix Trichosanthis and Fructus Forsythiae according the formulation, adding 7-10 times amount of water, decocting twice, 1-4 hour each time, filtering the water decoction, combining decoctions, concentrating the solution to obtain the extract with a relative density of 1.05-1.10 at 60°C, and combining the extract with extract of step b) for later use; and d)Mixing the fine powder of step a) and the extract of step c), adding an appropriate amount of adjuvant into the mixture, and spraying granulation to obtain granules and sifting the granules through a mesh after drying, standing by; and e) preparing the granules of step d} to obtain tablets of the traditional Chinese medicine composition through routine processes.
11. A preparation method of tablet of the traditional Chinese medicine composition according to claim 10, comprising the following steps: a) taking Radix Scutellariae and half amount of Rhizoma Pinelliae according to the formulation proportion, pulverizing them into fine powder for later use; b) taking the relative amounts of Herba Ephedrae, Semen Ginkgo, Cortex Mori, Flos Farfarae, Fructus Perillae, Semen Armeniacae Amarum and Herba Houttuyniae according to the formulation and the rest of Rhizoma Pinelliae, adding 8 times amount of 60% ethanol, heating and refluxing extract thrice, 1.5 hours each time, recovering ethanol under reduced pressure from extract solution, concentrating the solution to obtain the extract with a relative density of 1.05-1.10 at 60 °C for later use; ¢) taking the relative amounts of Lumbricus, Radix Paeoniae Rubra, Periostracum Cicadae, Radix Trichosanthis and Fructus Forsythiae according to the formulation, adding 9 times amount of water, decocting twice, 2 hour each time, filtering the water decoction, combining decoctions, concentrating the solution to obtain the extract with a relative density of 1.05-1.10 at 60°C, and combining the extract with extract of step b) for later use; and d)Mixing the fine powder of step a) and the extract of step ¢), adding an appropriate amount of adjuvant into the mixture, and spraying granulation to obtain granules and sifting the granules through a mesh after drying, standing by. ; and ¢) preparing the granules of step d) to obtain tablets of the traditional Chinese medicine composition through routine processes.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/CN2009/000781 WO2011003221A1 (en) | 2009-07-09 | 2009-07-09 | A medicine composition for treating bronchial asthma and preparative method thereof |
Publications (1)
Publication Number | Publication Date |
---|---|
SG177294A1 true SG177294A1 (en) | 2012-02-28 |
Family
ID=43428722
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG2011094356A SG177294A1 (en) | 2009-07-09 | 2009-07-09 | Traditional chinese medicine composition to treat bronchial asthma and preparation method thereof |
Country Status (4)
Country | Link |
---|---|
KR (1) | KR101416453B1 (en) |
RU (1) | RU2519672C2 (en) |
SG (1) | SG177294A1 (en) |
WO (1) | WO2011003221A1 (en) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102526394A (en) * | 2012-02-02 | 2012-07-04 | 南京中医药大学 | External use medicine for preventing and treating infantile asthma as well as acupoint application and preparation method thereof |
CN102688333B (en) * | 2012-06-14 | 2014-05-07 | 李良 | Traditional Chinese medicine decoction of snakegourd seed for treating cough and preparation method |
CN102716246A (en) * | 2012-06-18 | 2012-10-10 | 荣成市商贸城卫生所 | Traditional Chinese medicine composite for treating wind-heat type common cold |
CN103705581B (en) * | 2013-12-30 | 2016-01-13 | 长沙理工大学 | Compound Huodan dispersible tablet and its preparation method and application |
CN113876844B (en) * | 2021-10-11 | 2022-07-15 | 山南市藏医医院 | Pure traditional Chinese medicine Tibetan medicine for treating chronic tracheitis and preparation method and application thereof |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1294941C (en) * | 2004-05-27 | 2007-01-17 | 江西省药物研究所 | Chinese compound medicinal preparation for treating asthma and method for preparing the same |
RU2288741C2 (en) * | 2005-02-07 | 2006-12-10 | ГОУ ВПО "Воронежская государственная медицинская академия им. Н.Н. Бурденко Министерства здравоохранения Российской Федерации" | Method for treating bronchial asthma cases |
CN101229236A (en) | 2008-02-25 | 2008-07-30 | 北京金方华医药科技有限公司 | Relieve asthma oral liquid preparation and preparing method thereof |
CN101549059B (en) * | 2008-04-01 | 2011-09-14 | 河北以岭医药研究院有限公司 | Medicament for treating bronchial asthma and preparation method thereof |
-
2009
- 2009-07-09 WO PCT/CN2009/000781 patent/WO2011003221A1/en active Application Filing
- 2009-07-09 SG SG2011094356A patent/SG177294A1/en unknown
- 2009-07-09 KR KR1020127002997A patent/KR101416453B1/en active IP Right Grant
- 2009-07-09 RU RU2011146425/15A patent/RU2519672C2/en active
Also Published As
Publication number | Publication date |
---|---|
KR20120039688A (en) | 2012-04-25 |
RU2519672C2 (en) | 2014-06-20 |
WO2011003221A1 (en) | 2011-01-13 |
KR101416453B1 (en) | 2014-07-08 |
RU2011146425A (en) | 2013-08-20 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2012027882A1 (en) | Pharmaceutical composition for treating insomnia and preparation method thereof | |
EP2450046A1 (en) | A medicinal composition for the treatment of bronchitis and preparation thereof | |
SG177294A1 (en) | Traditional chinese medicine composition to treat bronchial asthma and preparation method thereof | |
WO2022237842A1 (en) | Pharmaceutical composition for treating rheumatoid arthritis and preparation method therefor | |
CN101041049A (en) | Chinese traditional medicine compound took orally for treating chronic bronchitis | |
CN106890269B (en) | External traditional Chinese medicine composition for treating chemotherapy drug induced peripheral neuropathy and application thereof | |
CN101549059B (en) | Medicament for treating bronchial asthma and preparation method thereof | |
CN101564458A (en) | Application of Chinese medicinal composition in preparing medicament for treating bronchitis | |
KR101286465B1 (en) | A composition comprising mixed herbal extract of Cnidii Rhizoma and Corydalis Tuber for treating or preventing respiratory disease | |
CN101024004B (en) | Medicine composition for treating respiratory tract system diseases and its preparation process | |
CN105796764B (en) | Preparation method and application of negundo chastetree fruit total lignans | |
CN114632129A (en) | Traditional Chinese medicine for treating cough variant asthma in children and preparation method thereof | |
CN102274428B (en) | Pharmaceutical composition with effect on treating irritable bowel syndrome and preparation method and application thereof | |
CN106138932A (en) | For treating the Chinese medicine of infantile asthma | |
CN111419894A (en) | Pharmaceutical composition for reducing uric acid and preparation method thereof | |
CN114588233B (en) | Composition for treating acute gout | |
CN112694441B (en) | C 20 Diterpenoid alkaloids, their preparation and use for treating pain related diseases | |
CN111939263B (en) | Pharmaceutical composition, traditional Chinese medicine composition, extract thereof, preparation, medicament and application | |
CN101972393B (en) | Fritillaria imperalis traditional Chinese medicine (TCM) composition for treating asthma and preparation method thereof | |
CN101549051A (en) | Traditional Chinese medicine composition for treating asthma and preparation method thereof | |
Wasiullah et al. | A REVIEW ON HERBAL TREATMENT OF ASTHMA AND COPD | |
CN104784567B (en) | A kind of pharmaceutical composition for the treatment of urinary system infection | |
CN114558066A (en) | Pharmaceutical composition for treating cough variant asthma and preparation method thereof | |
Wang et al. | Research Progress on Recognition and Treatment of RSV Infection in Traditional Chinese Medicine | |
Zhang et al. | Using Traditional Chinese Medicine to Alleviate Chronic Obstructive Pulmonary Disease |