SG11202111995TA - Methods of generating enucleated erythroid cells - Google Patents

Methods of generating enucleated erythroid cells

Info

Publication number
SG11202111995TA
SG11202111995TA SG11202111995TA SG11202111995TA SG11202111995TA SG 11202111995T A SG11202111995T A SG 11202111995TA SG 11202111995T A SG11202111995T A SG 11202111995TA SG 11202111995T A SG11202111995T A SG 11202111995TA SG 11202111995T A SG11202111995T A SG 11202111995TA
Authority
SG
Singapore
Prior art keywords
methods
erythroid cells
enucleated erythroid
generating
generating enucleated
Prior art date
Application number
SG11202111995TA
Inventor
Jonathan Lipsitz
Brian Joseph Pereira
Bryan Wang
Alan Gilbert
Original Assignee
Rubius Therapeutics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rubius Therapeutics Inc filed Critical Rubius Therapeutics Inc
Publication of SG11202111995TA publication Critical patent/SG11202111995TA/en

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
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    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/06—Tubular
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    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
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    • C12M23/00—Constructional details, e.g. recesses, hinges
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    • C12M23/14—Bags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
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    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
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    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
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    • C12N5/0641—Erythrocytes
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    • C12N2500/10—Metals; Metal chelators
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    • C12N2500/30—Organic components
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2303—Interleukin-3 (IL-3)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2306—Interleukin-6 (IL-6)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/26—Flt-3 ligand (CD135L, flk-2 ligand)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/33—Insulin
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
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    • C12N2521/00—Culture process characterised by the use of hydrostatic pressure, flow or shear forces
SG11202111995TA 2019-05-24 2020-05-22 Methods of generating enucleated erythroid cells SG11202111995TA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962852930P 2019-05-24 2019-05-24
PCT/US2020/034387 WO2020243006A1 (en) 2019-05-24 2020-05-22 Methods of generating enucleated erythroid cells

Publications (1)

Publication Number Publication Date
SG11202111995TA true SG11202111995TA (en) 2021-12-30

Family

ID=72148208

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202111995TA SG11202111995TA (en) 2019-05-24 2020-05-22 Methods of generating enucleated erythroid cells

Country Status (10)

Country Link
US (1) US20200370016A1 (en)
EP (1) EP3976762A1 (en)
JP (1) JP2022533252A (en)
KR (1) KR20220016480A (en)
CN (1) CN114127252A (en)
AU (1) AU2020283752A1 (en)
CA (1) CA3141315A1 (en)
IL (1) IL288208A (en)
SG (1) SG11202111995TA (en)
WO (1) WO2020243006A1 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014183066A2 (en) 2013-05-10 2014-11-13 Whitehead Institute For Biomedical Research Protein modification of living cells using sortase
US10471099B2 (en) * 2013-05-10 2019-11-12 Whitehead Institute For Biomedical Research In vitro production of red blood cells with proteins comprising sortase recognition motifs
WO2015073746A2 (en) 2013-11-13 2015-05-21 Whitehead Institute For Biomedical Research 18f labeling of proteins using sortases
EP3583202A1 (en) * 2017-02-17 2019-12-25 Rubius Therapeutics, Inc. Functionalized erythroid cells
FR3114323A1 (en) * 2020-09-23 2022-03-25 Erypharm Method for producing cultured red blood cells
TW202241470A (en) 2021-01-08 2022-11-01 美商盧比亞斯治療公司 Methods of treating a tumor in a human subject
TW202241471A (en) 2021-01-08 2022-11-01 美商盧比亞斯治療公司 Methods of increasing nkp30-positive lymphocytes in a subject and uses thereof
TW202304482A (en) 2021-03-14 2023-02-01 美商盧比亞斯治療公司 Methods of increasing nkg2d-positive lymphocytes in a subject and uses thereof
FR3120876A1 (en) * 2021-03-19 2022-09-23 Erypharm Method for producing cultured cells requiring a supply of ferric iron
TW202317179A (en) 2021-06-03 2023-05-01 美商盧比亞斯治療公司 Methods of treating hpv16-positive or hpv16-associated cancer in a subject
FR3126713A1 (en) * 2021-09-08 2023-03-10 Erypharm Method for producing high-density culture cells
FR3136483A1 (en) * 2022-06-09 2023-12-15 Erypharm METHOD FOR CULTURE OF CELLS REQUIRING IRON SUPPLY

Family Cites Families (13)

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Publication number Priority date Publication date Assignee Title
US4327710A (en) 1980-06-18 1982-05-04 The United States Of America As Represented By The Secretary Of Agriculture Process for encapsulating additives in resealed erythrocytes for disseminating chemicals via the circulatory system
EP0517986B1 (en) 1991-06-14 1997-09-10 Communaute Economique Europeenne (Cee) Transformed erythrocytes, process for preparing the same, and their use in pharmaceutical compositions
EP0882448B1 (en) 1997-05-05 2005-01-12 DIDECO S.r.l. Method of encapsulating biologically active agents within erythrocytes and apparatus therefor
US6495351B2 (en) 2000-02-08 2002-12-17 Gendel Limited Loading system and method for using the same
US6455306B1 (en) * 2000-06-09 2002-09-24 Transcyte, Inc. Transfusable oxygenating composition
CN101045914A (en) * 2006-03-29 2007-10-03 中国人民解放军军事医学科学院野战输血研究所 Extracorporeal induction process for differentiating hemopoietic stem/ancestral cell into mature red blood cell and its application
US10471099B2 (en) 2013-05-10 2019-11-12 Whitehead Institute For Biomedical Research In vitro production of red blood cells with proteins comprising sortase recognition motifs
WO2014183066A2 (en) 2013-05-10 2014-11-13 Whitehead Institute For Biomedical Research Protein modification of living cells using sortase
WO2015073587A2 (en) 2013-11-18 2015-05-21 Rubius Therapeutics, Inc. Synthetic membrane-receiver complexes
FR3017299B1 (en) 2014-02-12 2018-05-18 Erytech Pharma PHARMACEUTICAL COMPOSITION COMPRISING ERYTHROCYTES ENCAPSULATING A PLP ENZYME AND ITS COFACTOR
TW202246486A (en) * 2014-06-09 2022-12-01 美商健臻公司 Seed train processes and uses thereof
US20180135012A1 (en) * 2015-05-13 2018-05-17 Rubius Therapeutics, Inc. Membrane-receiver complex therapeutics
US20190160102A1 (en) * 2017-11-03 2019-05-30 Rubius Therapeutics, Inc. Compositions and methods related to therapeutic cell systems for tumor growth inhibition

Also Published As

Publication number Publication date
IL288208A (en) 2022-01-01
CA3141315A1 (en) 2020-12-03
US20200370016A1 (en) 2020-11-26
KR20220016480A (en) 2022-02-09
AU2020283752A1 (en) 2021-12-23
WO2020243006A1 (en) 2020-12-03
EP3976762A1 (en) 2022-04-06
JP2022533252A (en) 2022-07-21
CN114127252A (en) 2022-03-01

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