EP4072570A4 - Methods for generating hematopoietic stem cells - Google Patents

Methods for generating hematopoietic stem cells

Info

Publication number
EP4072570A4
EP4072570A4 EP20899010.1A EP20899010A EP4072570A4 EP 4072570 A4 EP4072570 A4 EP 4072570A4 EP 20899010 A EP20899010 A EP 20899010A EP 4072570 A4 EP4072570 A4 EP 4072570A4
Authority
EP
European Patent Office
Prior art keywords
methods
stem cells
hematopoietic stem
generating hematopoietic
generating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP20899010.1A
Other languages
German (de)
French (fr)
Other versions
EP4072570A1 (en
Inventor
Dhvanit I Shah
Giorgia Scapin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Brigham and Womens Hospital Inc
Original Assignee
Brigham and Womens Hospital Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Brigham and Womens Hospital Inc filed Critical Brigham and Womens Hospital Inc
Publication of EP4072570A1 publication Critical patent/EP4072570A1/en
Publication of EP4072570A4 publication Critical patent/EP4072570A4/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70596—Molecules with a "CD"-designation not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/11—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from blood or immune system cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/28—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from vascular endothelial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration
EP20899010.1A 2019-12-09 2020-12-09 Methods for generating hematopoietic stem cells Pending EP4072570A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962945838P 2019-12-09 2019-12-09
PCT/US2020/063901 WO2021119061A1 (en) 2019-12-09 2020-12-09 Methods for generating hematopoietic stem cells

Publications (2)

Publication Number Publication Date
EP4072570A1 EP4072570A1 (en) 2022-10-19
EP4072570A4 true EP4072570A4 (en) 2024-02-14

Family

ID=76330487

Family Applications (1)

Application Number Title Priority Date Filing Date
EP20899010.1A Pending EP4072570A4 (en) 2019-12-09 2020-12-09 Methods for generating hematopoietic stem cells

Country Status (11)

Country Link
US (1) US20230041065A1 (en)
EP (1) EP4072570A4 (en)
JP (1) JP2023504573A (en)
KR (1) KR20220111317A (en)
CN (1) CN115066492A (en)
AU (1) AU2020402020A1 (en)
BR (1) BR112022011169A2 (en)
CA (1) CA3164120A1 (en)
IL (1) IL293685A (en)
MX (1) MX2022006994A (en)
WO (1) WO2021119061A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2021527397A (en) 2018-06-07 2021-10-14 ザ ブリガム アンド ウィメンズ ホスピタル インコーポレイテッドＴｈｅ Ｂｒｉｇｈａｍ ａｎｄ Ｗｏｍｅｎ’ｓ Ｈｏｓｐｉｔａｌ， Ｉｎｃ． Methods for producing hematopoietic stem cells

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160032317A1 (en) * 2013-03-14 2016-02-04 Children's Medical Center Corporation Compositions and methods for reprogramming hematopoietic stem cell lineages
WO2016201133A2 (en) * 2015-06-09 2016-12-15 The Regents Of The University Of California Hematopoietic cells and methods of using and generating the same
WO2017096215A1 (en) * 2015-12-03 2017-06-08 The Brigham And Women's Hospital, Inc. Methods for generating functional hematopoietic stem cells
WO2019236943A2 (en) * 2018-06-07 2019-12-12 The Brigham And Women's Hospital, Inc. Methods for generating hematopoietic stem cells

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2010278738A1 (en) * 2009-07-31 2012-02-16 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Antiangiogenic small molecules and methods of use
US9528087B2 (en) * 2009-10-31 2016-12-27 Genesis Technologies Limited Methods for reprogramming cells and uses thereof
WO2012044696A2 (en) * 2010-09-30 2012-04-05 The Board Of Trustees Of The Leland Stanford Junior University Prediction of clinical outcome in hematological malignancies using a self-renewal expression signature
WO2012109208A2 (en) * 2011-02-08 2012-08-16 Cellular Dynamics International, Inc. Hematopoietic precursor cell production by programming

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160032317A1 (en) * 2013-03-14 2016-02-04 Children's Medical Center Corporation Compositions and methods for reprogramming hematopoietic stem cell lineages
WO2016201133A2 (en) * 2015-06-09 2016-12-15 The Regents Of The University Of California Hematopoietic cells and methods of using and generating the same
WO2017096215A1 (en) * 2015-12-03 2017-06-08 The Brigham And Women's Hospital, Inc. Methods for generating functional hematopoietic stem cells
WO2019236943A2 (en) * 2018-06-07 2019-12-12 The Brigham And Women's Hospital, Inc. Methods for generating hematopoietic stem cells
EP3802790A2 (en) * 2018-06-07 2021-04-14 The Brigham and Women's Hospital, Inc. Methods for generating hematopoietic stem cells

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
SCAPIN GIORGIA ET AL: "Piezo1-Sensitive Biomechanical Pulsation Stimulates Long-Term Hematopoietic Stem Cell Formation", BLOOD, AMERICAN SOCIETY OF HEMATOLOGY, US, vol. 132, 29 November 2018 (2018-11-29), pages 3826, XP086592966, ISSN: 0006-4971, DOI: 10.1182/BLOOD-2018-99-110265 *
SCAPIN GIORGIA ET AL: "Pulsation Activates Mechanosensitive Piezo1 to Form Long-Term Hematopoietic Stem Cells", BLOOD, AMERICAN SOCIETY OF HEMATOLOGY, US, vol. 134, 445, 13 November 2019 (2019-11-13), pages 1 - 2, XP086674239, ISSN: 0006-4971, DOI: 10.1182/BLOOD-2019-121948 *
See also references of WO2021119061A1 *

Also Published As

Publication number Publication date
WO2021119061A1 (en) 2021-06-17
BR112022011169A2 (en) 2022-09-27
CN115066492A (en) 2022-09-16
IL293685A (en) 2022-08-01
EP4072570A1 (en) 2022-10-19
CA3164120A1 (en) 2021-06-17
KR20220111317A (en) 2022-08-09
AU2020402020A1 (en) 2022-06-23
US20230041065A1 (en) 2023-02-09
MX2022006994A (en) 2022-09-29
JP2023504573A (en) 2023-02-03

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