SG11202106370WA - Method and system of identifying and quantifying antibody fragmentation - Google Patents

Method and system of identifying and quantifying antibody fragmentation

Info

Publication number
SG11202106370WA
SG11202106370WA SG11202106370WA SG11202106370WA SG11202106370WA SG 11202106370W A SG11202106370W A SG 11202106370WA SG 11202106370W A SG11202106370W A SG 11202106370WA SG 11202106370W A SG11202106370W A SG 11202106370WA SG 11202106370W A SG11202106370W A SG 11202106370WA
Authority
SG
Singapore
Prior art keywords
identifying
antibody fragmentation
quantifying antibody
quantifying
fragmentation
Prior art date
Application number
SG11202106370WA
Other languages
English (en)
Inventor
Yuetian Yan
Shunhai Wang
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of SG11202106370WA publication Critical patent/SG11202106370WA/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • G01N30/724—Nebulising, aerosol formation or ionisation
    • G01N30/7266—Nebulising, aerosol formation or ionisation by electric field, e.g. electrospray
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G01N33/6857—Antibody fragments
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/563—Immunoassay; Biospecific binding assay; Materials therefor involving antibody fragments
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biophysics (AREA)
  • Dispersion Chemistry (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
SG11202106370WA 2019-01-16 2020-01-15 Method and system of identifying and quantifying antibody fragmentation SG11202106370WA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962793004P 2019-01-16 2019-01-16
PCT/US2020/013648 WO2020150328A1 (en) 2019-01-16 2020-01-15 Method and system of identifying and quantifying antibody fragmentation

Publications (1)

Publication Number Publication Date
SG11202106370WA true SG11202106370WA (en) 2021-07-29

Family

ID=69529055

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202106370WA SG11202106370WA (en) 2019-01-16 2020-01-15 Method and system of identifying and quantifying antibody fragmentation

Country Status (13)

Country Link
US (2) US11486864B2 (zh)
EP (1) EP3911947A1 (zh)
JP (1) JP2022517338A (zh)
KR (1) KR20210116502A (zh)
CN (1) CN113227785A (zh)
AU (1) AU2020208360A1 (zh)
BR (1) BR112021011194A2 (zh)
CA (1) CA3123052A1 (zh)
EA (1) EA202191961A1 (zh)
IL (1) IL284637A (zh)
MX (1) MX2021008558A (zh)
SG (1) SG11202106370WA (zh)
WO (1) WO2020150328A1 (zh)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11385239B2 (en) * 2018-10-31 2022-07-12 Regeneran Pharmaceuticals, Inc. Method and system of identifying and quantifying a protein
AU2021286463A1 (en) * 2020-06-09 2022-12-22 Regeneron Pharmaceuticals, Inc. A high-throughput and mass-spectrometry-based method for quantitating antibodies
US20230018713A1 (en) * 2021-07-13 2023-01-19 Regeneron Pharmaceuticals, Inc. Characterization of proteins by anion-exchange chromatography mass spectrometry (aex-ms)

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2493817A1 (en) * 2002-08-06 2004-02-12 The John Hopkins University Use of biomarkers for detecting ovarian cancer
WO2007038523A2 (en) * 2005-09-27 2007-04-05 Center For Applied Proteomics And Molecular Medicine Method of isolating analytes from a sample
MX2015006868A (es) * 2012-11-30 2015-10-05 Novartis Ag Metodos para la elaboracion de conjugados a partir de proteinas que contienen disulfuro.
US11092574B2 (en) 2013-12-24 2021-08-17 Waters Technologies Corporation Materials for hydrophilic interaction chromatography and processes for preparation and use thereof for analysis of glycoproteins and glycopeptides
WO2016018978A1 (en) 2014-07-29 2016-02-04 Mayo Foundation For Medical Education And Research Quantifying monoclonal antibody therapeutics by lc-ms/ms
WO2016149088A1 (en) * 2015-03-13 2016-09-22 Bristol-Myers Squibb Company Use of alkaline washes during chromatography to remove impurities
CA3013340A1 (en) * 2016-02-04 2017-08-10 Oncobiologics, Inc. Methods for identifying and analyzing amino acid sequences of proteins
MX2019000935A (es) * 2016-08-16 2019-07-04 Regeneron Pharma Metodos para cuantificar anticuerpos individuales de una mezcla.
US11385239B2 (en) * 2018-10-31 2022-07-12 Regeneran Pharmaceuticals, Inc. Method and system of identifying and quantifying a protein

Also Published As

Publication number Publication date
BR112021011194A2 (pt) 2021-08-31
KR20210116502A (ko) 2021-09-27
US20200225199A1 (en) 2020-07-16
US20230314388A1 (en) 2023-10-05
US12000811B2 (en) 2024-06-04
US11486864B2 (en) 2022-11-01
CA3123052A1 (en) 2020-07-23
EP3911947A1 (en) 2021-11-24
CN113227785A (zh) 2021-08-06
IL284637A (en) 2021-08-31
MX2021008558A (es) 2021-08-19
AU2020208360A1 (en) 2021-07-01
EA202191961A1 (ru) 2021-12-13
JP2022517338A (ja) 2022-03-08
WO2020150328A1 (en) 2020-07-23

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