SG11202104701XA - Methods, compositions, and systems for improving recovery of nucleic acid molecules - Google Patents

Methods, compositions, and systems for improving recovery of nucleic acid molecules

Info

Publication number
SG11202104701XA
SG11202104701XA SG11202104701XA SG11202104701XA SG11202104701XA SG 11202104701X A SG11202104701X A SG 11202104701XA SG 11202104701X A SG11202104701X A SG 11202104701XA SG 11202104701X A SG11202104701X A SG 11202104701XA SG 11202104701X A SG11202104701X A SG 11202104701XA
Authority
SG
Singapore
Prior art keywords
compositions
systems
methods
nucleic acid
acid molecules
Prior art date
Application number
SG11202104701XA
Other languages
English (en)
Inventor
Stefanie Ann Ward Mortimer
Dustin Howard Hite
Laura Michelle Melroy
Original Assignee
Guardant Health Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guardant Health Inc filed Critical Guardant Health Inc
Publication of SG11202104701XA publication Critical patent/SG11202104701XA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50Detection characterised by immobilisation to a surface
    • C12Q2565/514Detection characterised by immobilisation to a surface characterised by the use of the arrayed oligonucleotides as identifier tags, e.g. universal addressable array, anti-tag or tag complement array
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
SG11202104701XA 2018-12-20 2019-12-20 Methods, compositions, and systems for improving recovery of nucleic acid molecules SG11202104701XA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862783046P 2018-12-20 2018-12-20
PCT/US2019/068146 WO2020132628A1 (en) 2018-12-20 2019-12-20 Methods, compositions, and systems for improving recovery of nucleic acid molecules

Publications (1)

Publication Number Publication Date
SG11202104701XA true SG11202104701XA (en) 2021-06-29

Family

ID=69326689

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202104701XA SG11202104701XA (en) 2018-12-20 2019-12-20 Methods, compositions, and systems for improving recovery of nucleic acid molecules

Country Status (7)

Country Link
US (2) US20200232010A1 (https=)
EP (1) EP3898969A1 (https=)
JP (2) JP2022514010A (https=)
CN (1) CN113454218A (https=)
CA (1) CA3119980A1 (https=)
SG (1) SG11202104701XA (https=)
WO (1) WO2020132628A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111944806A (zh) * 2020-07-30 2020-11-17 上海韦翰斯生物医药科技有限公司 一种高通量测序污染检测用分子标签组及其应用
JP2024513187A (ja) * 2021-03-29 2024-03-22 イルミナ インコーポレイテッド ライブラリー中のdna損傷を評価し、アンプリコンサイズバイアスを正規化するための組成物及び方法
CN118139987A (zh) 2021-09-23 2024-06-04 基因组检测合作社公司 用于cfrna和cftna靶向ngs测序的组合物和方法
CN115710751A (zh) * 2022-11-09 2023-02-24 石家庄冰缘医学检验实验室有限公司 一种多重pcr建库防止污染的方法

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6582908B2 (en) 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
US20030017081A1 (en) 1994-02-10 2003-01-23 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
DE69528706T2 (de) 1994-08-19 2003-06-12 Pe Corp. (Ny), Foster City Gekoppeltes ampflikation- und ligationverfahren
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
AU7537200A (en) 1999-09-29 2001-04-30 Solexa Ltd. Polynucleotide sequencing
ATE377093T1 (de) 2000-07-07 2007-11-15 Visigen Biotechnologies Inc Sequenzbestimmung in echtzeit
WO2003046146A2 (en) 2001-11-28 2003-06-05 Applera Corporation Compositions and methods of selective nucleic acid isolation
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
CA2579150C (en) 2004-09-17 2014-11-25 Pacific Biosciences Of California, Inc. Apparatus and method for analysis of molecules
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US7282337B1 (en) 2006-04-14 2007-10-16 Helicos Biosciences Corporation Methods for increasing accuracy of nucleic acid sequencing
US20120208711A1 (en) * 2009-10-02 2012-08-16 Centre For Addiction And Mental Health Method for Analysis of DNA Methylation Profiles of Cell-Free Circulating DNA in Bodily Fluids
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
CN104781421B (zh) 2012-09-04 2020-06-05 夸登特健康公司 检测稀有突变和拷贝数变异的系统和方法
WO2015073080A1 (en) * 2013-11-12 2015-05-21 Life Technologies Corporation Reagents and methods for sequencing
ES2660989T3 (es) 2013-12-28 2018-03-27 Guardant Health, Inc. Métodos y sistemas para detectar variantes genéticas
EP3502273B1 (en) * 2014-12-12 2020-07-08 Verinata Health, Inc. Cell-free dna fragment
JP6873921B2 (ja) * 2015-05-18 2021-05-19 カリウス・インコーポレイテッド 核酸の集団を濃縮するための組成物および方法
US10095831B2 (en) * 2016-02-03 2018-10-09 Verinata Health, Inc. Using cell-free DNA fragment size to determine copy number variations
EP3978627A1 (en) * 2016-03-25 2022-04-06 Karius, Inc. Methods using synthetic nucleic acid spike-ins
CA3023248A1 (en) * 2016-05-06 2017-11-09 Regents Of The University Of Minnesota Analytical standards and methods of using same
MX2019007444A (es) * 2016-12-22 2019-08-16 Guardant Health Inc Metodos y sistemas para analisis de moleculas de acido nucleico.

Also Published As

Publication number Publication date
EP3898969A1 (en) 2021-10-27
JP2025106543A (ja) 2025-07-15
JP2022514010A (ja) 2022-02-09
CN113454218A (zh) 2021-09-28
US20260035743A1 (en) 2026-02-05
US20200232010A1 (en) 2020-07-23
WO2020132628A1 (en) 2020-06-25
CA3119980A1 (en) 2020-06-25

Similar Documents

Publication Publication Date Title
IL267424A (en) Methods and systems for analyzing nucleic acid molecules
EP3775272A4 (en) Methods, systems, and compositions for counting nucleic acid molecules
SG11202108788TA (en) Methods for processing nucleic acid molecules
ZA202001294B (en) Multispecific antibody
GB2627085B (en) Methods and systems for analysing nucleic acid molecules
IL259736A (en) Methods and systems for nucleic acid analysis
WO2016065349A8 (en) Short non-coding protein regulatory rnas (sprrnas) and methods of use
EP3271848A4 (en) Systems and methods for analyzing nucleic acid
EP3234200A4 (en) Compositions and methods for targeted depletion, enrichment, and partitioning of nucleic acids using crispr/cas system proteins
IL277472A (en) Nucleic acid molecules for pseudouridylation
WO2015196128A3 (en) Alternative nucleic acid molecules and uses thereof
WO2015196130A3 (en) Alternative nucleic acid molecules and uses thereof
EP3161700A4 (en) Processes and systems for nucleic acid sequence assembly
WO2015119941A3 (en) Genome fractioning
MX2016007541A (es) Moléculas modificadas de ácido nucleico y usos de las mismas.
IL273426A (en) Systems and methods for nucleic acid sequencing
IL262019A (en) Nucleic acid stabilizing reagent, kits and methods for its use
SG11202104701XA (en) Methods, compositions, and systems for improving recovery of nucleic acid molecules
EP3283630A4 (en) Oligonucleotide fragment, and method as well as application of selective amplifiction of variant of target nucleic acid sequence using the same
HK1246690A1 (zh) 用於鉴定高度特异性核酸酶的方法和组合物
CA3276585A1 (en) Methods for preparing nucleic acid molecules for sequencing
EP3268496A4 (en) Graphene fet devices, systems, and methods of using the same for sequencing nucleic acids
IL260122A (en) Methods and compositions for assembly of nucleic acids
EP3289356A4 (en) Nucleic acid detection and quantification method and compositions
CA3278536A1 (en) Systems and processes for rapid nucleic acid extraction, purification, and analysis