JP2022514010A - 核酸分子の回収率を改善するための方法、組成物、およびシステム - Google Patents

核酸分子の回収率を改善するための方法、組成物、およびシステム Download PDF

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JP2022514010A
JP2022514010A JP2021535033A JP2021535033A JP2022514010A JP 2022514010 A JP2022514010 A JP 2022514010A JP 2021535033 A JP2021535033 A JP 2021535033A JP 2021535033 A JP2021535033 A JP 2021535033A JP 2022514010 A JP2022514010 A JP 2022514010A
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fragment size
sample
subset
size control
molecules
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ステファニー アン ウォード モーティマー，
ダスティン ハワード ハイト，
ローラ ミシェル メルロイ，
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ガーダント ヘルス， インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/514—Detection characterised by immobilisation to a surface characterised by the use of the arrayed oligonucleotides as identifier tags, e.g. universal addressable array, anti-tag or tag complement array
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
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  • Crystallography & Structural Chemistry (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2021535033A 2018-12-20 2019-12-20 核酸分子の回収率を改善するための方法、組成物、およびシステム Pending JP2022514010A (ja)

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JP2025066850A JP2025106543A (ja) 2018-12-20 2025-04-15 核酸分子の回収率を改善するための方法、組成物、およびシステム

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US201862783046P 2018-12-20 2018-12-20
US62/783,046 2018-12-20
PCT/US2019/068146 WO2020132628A1 (en) 2018-12-20 2019-12-20 Methods, compositions, and systems for improving recovery of nucleic acid molecules

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JP2022514010A true JP2022514010A (ja) 2022-02-09
JP2022514010A5 JP2022514010A5 (https=) 2023-02-17

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JP2025066850A Pending JP2025106543A (ja) 2018-12-20 2025-04-15 核酸分子の回収率を改善するための方法、組成物、およびシステム

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US (2) US20200232010A1 (https=)
EP (1) EP3898969A1 (https=)
JP (2) JP2022514010A (https=)
CN (1) CN113454218A (https=)
CA (1) CA3119980A1 (https=)
SG (1) SG11202104701XA (https=)
WO (1) WO2020132628A1 (https=)

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CN111944806A (zh) * 2020-07-30 2020-11-17 上海韦翰斯生物医药科技有限公司 一种高通量测序污染检测用分子标签组及其应用
JP2024513187A (ja) * 2021-03-29 2024-03-22 イルミナ インコーポレイテッド ライブラリー中のｄｎａ損傷を評価し、アンプリコンサイズバイアスを正規化するための組成物及び方法
CN118139987A (zh) 2021-09-23 2024-06-04 基因组检测合作社公司 用于cfrna和cftna靶向ngs测序的组合物和方法
CN115710751A (zh) * 2022-11-09 2023-02-24 石家庄冰缘医学检验实验室有限公司 一种多重pcr建库防止污染的方法

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WO2017192974A1 (en) * 2016-05-06 2017-11-09 Regents Of The University Of Minnesota Analytical standards and methods of using same

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DE69528706T2 (de) 1994-08-19 2003-06-12 Pe Corp. (Ny), Foster City Gekoppeltes ampflikation- und ligationverfahren
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
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US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
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EP3898969A1 (en) 2021-10-27
SG11202104701XA (en) 2021-06-29
JP2025106543A (ja) 2025-07-15
CN113454218A (zh) 2021-09-28
US20260035743A1 (en) 2026-02-05
US20200232010A1 (en) 2020-07-23
WO2020132628A1 (en) 2020-06-25
CA3119980A1 (en) 2020-06-25

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