SG11202103722TA - Programmable dna base editing by nme2cas9-deaminase fusion proteins - Google Patents
Programmable dna base editing by nme2cas9-deaminase fusion proteinsInfo
- Publication number
- SG11202103722TA SG11202103722TA SG11202103722TA SG11202103722TA SG11202103722TA SG 11202103722T A SG11202103722T A SG 11202103722TA SG 11202103722T A SG11202103722T A SG 11202103722TA SG 11202103722T A SG11202103722T A SG 11202103722TA SG 11202103722T A SG11202103722T A SG 11202103722TA
- Authority
- SG
- Singapore
- Prior art keywords
- nme2cas9
- fusion proteins
- dna base
- base editing
- programmable dna
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
- C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
- C12Y305/04004—Adenosine deaminase (3.5.4.4)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
- C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
- C12Y305/04005—Cytidine deaminase (3.5.4.5)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
- C12N2750/14142—Use of virus, viral particle or viral elements as a vector virus or viral particle as vehicle, e.g. encapsulating small organic molecule
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14171—Demonstrated in vivo effect
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862745666P | 2018-10-15 | 2018-10-15 | |
| PCT/US2019/056341 WO2020081568A1 (en) | 2018-10-15 | 2019-10-15 | Programmable dna base editing by nme2cas9-deaminase fusion proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11202103722TA true SG11202103722TA (en) | 2021-05-28 |
Family
ID=68621349
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11202103722TA SG11202103722TA (en) | 2018-10-15 | 2019-10-15 | Programmable dna base editing by nme2cas9-deaminase fusion proteins |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US20220290113A1 (pt) |
| EP (1) | EP3867367A1 (pt) |
| JP (1) | JP2022508716A (pt) |
| KR (1) | KR20210077732A (pt) |
| CN (1) | CN113166743A (pt) |
| AU (1) | AU2019362874A1 (pt) |
| BR (1) | BR112021007123A2 (pt) |
| CA (1) | CA3116555A1 (pt) |
| CO (1) | CO2021006336A2 (pt) |
| EA (1) | EA202191033A1 (pt) |
| IL (1) | IL282267A (pt) |
| MX (1) | MX2021004301A (pt) |
| SG (1) | SG11202103722TA (pt) |
| WO (1) | WO2020081568A1 (pt) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2021267940A1 (en) | 2020-05-08 | 2022-12-08 | President And Fellows Of Harvard College | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence |
| GB202010348D0 (en) | 2020-07-06 | 2020-08-19 | Univ Wageningen | Base editing tools |
| WO2022125968A1 (en) | 2020-12-11 | 2022-06-16 | Intellia Therapeutics, Inc. | Polynucleotides, compositions, and methods for genome editing involving deamination |
| AU2021396403A1 (en) | 2020-12-11 | 2023-06-29 | Intellia Therapeutics, Inc. | Compositions and methods for reducing mhc class ii in a cell |
| WO2022140587A1 (en) | 2020-12-23 | 2022-06-30 | Intellia Therapeutics, Inc. | Compositions and methods for genetically modifying ciita in a cell |
| CR20230320A (es) | 2020-12-23 | 2023-10-23 | Intellia Therapeutics Inc | Composiciones y métodos para reducir los hla-a en una célula |
| WO2023081070A1 (en) * | 2021-11-02 | 2023-05-11 | University Of Massachusetts | Nme2Cas9 INLAID DOMAIN FUSION PROTEINS |
| AU2022382975A1 (en) | 2021-11-03 | 2024-05-02 | Intellia Therapeutics, Inc. | Polynucleotides, compositions, and methods for genome editing |
| WO2023196802A1 (en) * | 2022-04-04 | 2023-10-12 | The Broad Institute, Inc. | Cas9 variants having non-canonical pam specificities and uses thereof |
| WO2023227669A2 (en) * | 2022-05-26 | 2023-11-30 | UCB Biopharma SRL | Novel nucleic acid-editing proteins |
| WO2023245113A1 (en) | 2022-06-16 | 2023-12-21 | Intellia Therapeutics, Inc. | Methods and compositions for genetically modifying a cell |
| WO2023245109A2 (en) | 2022-06-16 | 2023-12-21 | Intellia Therapeutics, Inc. | Compositions and methods for genomic editing |
| WO2023245108A2 (en) | 2022-06-16 | 2023-12-21 | Intellia Therapeutics, Inc. | Compositions and methods for reducing mhc class i in a cell |
| CN115206456B (zh) * | 2022-07-13 | 2023-04-25 | 黑龙江大学 | 基于属性编辑流的分子生成方法 |
| CN115851775B (zh) * | 2022-10-18 | 2023-08-04 | 哈尔滨工业大学 | 一种Cas9蛋白抑制剂及其应用 |
| CN115716880A (zh) * | 2022-12-07 | 2023-02-28 | 云舟生物科技(广州)股份有限公司 | 一种核定位荧光蛋白及其应用 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL154598B (nl) | 1970-11-10 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking. |
| US3817837A (en) | 1971-05-14 | 1974-06-18 | Syva Corp | Enzyme amplification assay |
| US3939350A (en) | 1974-04-29 | 1976-02-17 | Board Of Trustees Of The Leland Stanford Junior University | Fluorescent immunoassay employing total reflection for activation |
| US3996345A (en) | 1974-08-12 | 1976-12-07 | Syva Company | Fluorescence quenching with immunological pairs in immunoassays |
| US4277437A (en) | 1978-04-05 | 1981-07-07 | Syva Company | Kit for carrying out chemically induced fluorescence immunoassay |
| US4275149A (en) | 1978-11-24 | 1981-06-23 | Syva Company | Macromolecular environment control in specific receptor assays |
| US4366241A (en) | 1980-08-07 | 1982-12-28 | Syva Company | Concentrating zone method in heterogeneous immunoassays |
| KR101780885B1 (ko) * | 2013-03-14 | 2017-10-11 | 카리부 바이오사이언시스 인코포레이티드 | 핵산-표적화 핵산의 조성물 및 방법 |
| US20150165054A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting caspase-9 point mutations |
| EP3365357B1 (en) | 2015-10-23 | 2024-02-14 | President and Fellows of Harvard College | Evolved cas9 proteins for gene editing |
| AU2017306676B2 (en) * | 2016-08-03 | 2024-02-22 | President And Fellows Of Harvard College | Adenosine nucleobase editors and uses thereof |
| WO2018149915A1 (en) * | 2017-02-15 | 2018-08-23 | Keygene N.V. | Methods of targeted genetic alteration in plant cells |
| WO2018152197A1 (en) * | 2017-02-15 | 2018-08-23 | Massachusetts Institute Of Technology | Dna writers, molecular recorders and uses thereof |
| IL306092A (en) * | 2017-03-23 | 2023-11-01 | Harvard College | Nucleic base editors that include nucleic acid programmable DNA binding proteins |
-
2019
- 2019-10-15 US US17/285,440 patent/US20220290113A1/en active Pending
- 2019-10-15 CA CA3116555A patent/CA3116555A1/en active Pending
- 2019-10-15 SG SG11202103722TA patent/SG11202103722TA/en unknown
- 2019-10-15 AU AU2019362874A patent/AU2019362874A1/en active Pending
- 2019-10-15 BR BR112021007123-7A patent/BR112021007123A2/pt unknown
- 2019-10-15 KR KR1020217014669A patent/KR20210077732A/ko unknown
- 2019-10-15 JP JP2021545287A patent/JP2022508716A/ja active Pending
- 2019-10-15 WO PCT/US2019/056341 patent/WO2020081568A1/en active Application Filing
- 2019-10-15 EA EA202191033A patent/EA202191033A1/ru unknown
- 2019-10-15 EP EP19806344.8A patent/EP3867367A1/en active Pending
- 2019-10-15 MX MX2021004301A patent/MX2021004301A/es unknown
- 2019-10-15 CN CN201980077403.7A patent/CN113166743A/zh active Pending
-
2021
- 2021-04-12 IL IL282267A patent/IL282267A/en unknown
- 2021-05-14 CO CONC2021/0006336A patent/CO2021006336A2/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| BR112021007123A2 (pt) | 2021-08-10 |
| EA202191033A1 (ru) | 2021-12-21 |
| CO2021006336A2 (es) | 2021-10-29 |
| CA3116555A1 (en) | 2020-04-23 |
| MX2021004301A (es) | 2021-08-05 |
| WO2020081568A1 (en) | 2020-04-23 |
| US20220290113A1 (en) | 2022-09-15 |
| EP3867367A1 (en) | 2021-08-25 |
| KR20210077732A (ko) | 2021-06-25 |
| AU2019362874A1 (en) | 2021-05-27 |
| IL282267A (en) | 2021-05-31 |
| CN113166743A (zh) | 2021-07-23 |
| JP2022508716A (ja) | 2022-01-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| IL282267A (en) | DNA editing programmed by deaminase-nme2cas9 chimeric proteins | |
| EP3676287A4 (en) | FUSION PROTEINS FOR IMPROVED PRECISION IN BASE EDITING | |
| IL281954A (en) | Heterodimeric chimeric proteins | |
| HK1244019A1 (zh) | 融合蛋白 | |
| EP3755726A4 (en) | FUSION PROTEINS FOR BASIC EDITION | |
| SG11202011743SA (en) | Fusion proteins comprising progranulin | |
| HK1246315A1 (zh) | 細胞因子融合蛋白 | |
| IL281962A (en) | Proteins from IL-12 heterodimers-FC | |
| EP3743438A4 (en) | CYTOKI FUSION PROTEINS | |
| EP3880814A4 (en) | FUSION PROTEIN | |
| EP3757218A4 (en) | MERGER PROTEIN | |
| KR20220035471A (ko) | 면역치료법을 위한 융합 폴리펩티드 | |
| HK1258125A1 (zh) | 胰島素免疫球蛋白融合蛋白 | |
| SG11202101904UA (en) | Spd-1 variant - fc fusion proteins | |
| GB201504691D0 (en) | Fusion protein | |
| ZA202003845B (en) | Fusion proteins | |
| IL289413A (en) | Nucleic acid-directed enhanced cell editing via the lexa-rad51 fusion protein | |
| IL290660A (en) | Medical protein concentrates | |
| IL281088A (en) | FLT3L - based chimeric proteins | |
| ZA202102533B (en) | Fusion protein | |
| GB201708330D0 (en) | Fusion protein for enhancing intestinal regeneration | |
| GB201815670D0 (en) | Protein editing | |
| EP3464384A4 (en) | CONSTRUCTION OF FUSION PROTEINS | |
| EP4100444A4 (en) | MUCI-BINDING FUSION PROTEINS | |
| GB201602850D0 (en) | Fusion proteins |