SG11202103214TA - Multiplexed fluorescent detection of analytes - Google Patents

Multiplexed fluorescent detection of analytes

Info

Publication number
SG11202103214TA
SG11202103214TA SG11202103214TA SG11202103214TA SG11202103214TA SG 11202103214T A SG11202103214T A SG 11202103214TA SG 11202103214T A SG11202103214T A SG 11202103214TA SG 11202103214T A SG11202103214T A SG 11202103214TA SG 11202103214T A SG11202103214T A SG 11202103214TA
Authority
SG
Singapore
Prior art keywords
analytes
fluorescent detection
multiplexed fluorescent
multiplexed
detection
Prior art date
Application number
SG11202103214TA
Other languages
English (en)
Inventor
Nikolai Romanov
Stanley S Hong
Patrick Mccauley
Xiaohai Liu
Danilo Condello
Merek Siu
Original Assignee
Illumina Inc
Illumina Cambridge Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc, Illumina Cambridge Ltd filed Critical Illumina Inc
Priority claimed from PCT/EP2020/055426 external-priority patent/WO2020178231A1/en
Publication of SG11202103214TA publication Critical patent/SG11202103214TA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01JMEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28Investigating the spectrum
    • G01J3/44Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G01J3/4406Fluorescence spectrometry
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01JMEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28Investigating the spectrum
    • G01J3/2823Imaging spectrometer
    • G01J2003/2826Multispectral imaging, e.g. filter imaging
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01JMEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28Investigating the spectrum
    • G01J3/30Measuring the intensity of spectral lines directly on the spectrum itself
    • G01J3/36Investigating two or more bands of a spectrum by separate detectors
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N2021/6417Spectrofluorimetric devices
    • G01N2021/6421Measuring at two or more wavelengths
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
SG11202103214TA 2019-03-01 2020-03-02 Multiplexed fluorescent detection of analytes SG11202103214TA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962812883P 2019-03-01 2019-03-01
NL2023327A NL2023327B1 (en) 2019-03-01 2019-06-17 Multiplexed fluorescent detection of analytes
PCT/EP2020/055426 WO2020178231A1 (en) 2019-03-01 2020-03-02 Multiplexed fluorescent detection of analytes

Publications (1)

Publication Number Publication Date
SG11202103214TA true SG11202103214TA (en) 2021-09-29

Family

ID=67660434

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202103214TA SG11202103214TA (en) 2019-03-01 2020-03-02 Multiplexed fluorescent detection of analytes

Country Status (13)

Country Link
US (1) US20220049292A1 (ja)
EP (1) EP3837384A1 (ja)
JP (1) JP2022522076A (ja)
KR (1) KR20210134599A (ja)
CN (1) CN112969802A (ja)
AU (1) AU2020231007A1 (ja)
BR (1) BR112021006428A2 (ja)
CA (1) CA3114733A1 (ja)
IL (1) IL281861A (ja)
MX (1) MX2021003777A (ja)
NL (1) NL2023327B1 (ja)
SG (1) SG11202103214TA (ja)
TW (1) TW202100751A (ja)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023192163A1 (en) * 2022-03-29 2023-10-05 Illumina Cambridge Limited Systems and methods of sequencing polynucleotides
CN115414703B (zh) * 2022-11-03 2023-01-17 百力格生物科技(上海)股份有限公司 染料修饰后的核酸探针纯化方法

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5268486A (en) 1986-04-18 1993-12-07 Carnegie-Mellon Unversity Method for labeling and detecting materials employing arylsulfonate cyanine dyes
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
ES2563643T3 (es) 1997-04-01 2016-03-15 Illumina Cambridge Limited Método de secuenciación de ácido nucleico
US6465178B2 (en) 1997-09-30 2002-10-15 Surmodics, Inc. Target molecule attachment to surfaces
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
GB0002310D0 (en) 2000-02-01 2000-03-22 Solexa Ltd Polynucleotide sequencing
IL141148A0 (en) 1998-07-30 2002-02-10 Solexa Ltd Arrayed biomolecules and their use in sequencing
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6391937B1 (en) 1998-11-25 2002-05-21 Motorola, Inc. Polyacrylamide hydrogels and hydrogel arrays made from polyacrylamide reactive prepolymers
US6664061B2 (en) 1999-06-25 2003-12-16 Amersham Biosciences Ab Use and evaluation of a [2+2] photoaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix
US6372813B1 (en) 1999-06-25 2002-04-16 Motorola Methods and compositions for attachment of biomolecules to solid supports, hydrogels, and hydrogel arrays
AU2001279244A1 (en) 2000-08-09 2002-02-18 Motorola, Inc. The use and evaluation of a (2+2) photocycloaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix
ATE352586T2 (de) 2000-09-29 2007-02-15 Molecular Probes Inc Modifizierte carbocyaninfarbstoffe und deren konjugate
DK3363809T3 (da) 2002-08-23 2020-05-04 Illumina Cambridge Ltd Modificerede nukleotider til polynukleotidsekvensering
ATE431354T1 (de) 2002-08-23 2009-05-15 Illumina Cambridge Ltd Markierte nukleotide
DE10258150A1 (de) 2002-12-10 2004-07-08 Dyomics Gmbh Hydrophile Marker auf der Basis von Benzopyrylo-Polymethinen
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
GB0326073D0 (en) 2003-11-07 2003-12-10 Solexa Ltd Improvements in or relating to polynucleotide arrays
EP1701785A1 (en) 2004-01-07 2006-09-20 Solexa Ltd. Modified molecular arrays
JP4990886B2 (ja) 2005-05-10 2012-08-01 ソレックサ リミテッド 改良ポリメラーゼ
GB0517097D0 (en) 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
US9017973B2 (en) * 2008-03-19 2015-04-28 Intelligent Biosystems, Inc. Methods and compositions for incorporating nucleotides
WO2013044018A1 (en) 2011-09-23 2013-03-28 Illumina, Inc. Methods and compositions for nucleic acid sequencing
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
ES2620423T3 (es) 2013-03-08 2017-06-28 Illumina Cambridge Limited Compuestos de polimetina y su uso como marcadores fluorescentes
CN105377869B (zh) 2013-03-15 2018-07-10 伊鲁米纳剑桥有限公司 修饰的核苷或核苷酸
CN103866010B (zh) * 2014-02-28 2016-04-20 郭诚 一种基因测序的方法
GB201414098D0 (en) 2014-08-08 2014-09-24 Illumina Cambridge Ltd Modified nucleotide linkers
GB201516987D0 (en) 2015-09-25 2015-11-11 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
US10385214B2 (en) 2016-09-30 2019-08-20 Illumina Cambridge Limited Fluorescent dyes and their uses as biomarkers
NZ778462A (en) * 2016-12-22 2024-02-23 Illumina Cambridge Ltd Coumarin compounds and their uses as fluorescent labels
GB201716931D0 (en) * 2017-10-16 2017-11-29 Illumina Cambridge Ltd New fluorescent compounds and their use as biomarkers

Also Published As

Publication number Publication date
US20220049292A1 (en) 2022-02-17
KR20210134599A (ko) 2021-11-10
CA3114733A1 (en) 2020-09-10
TW202100751A (zh) 2021-01-01
AU2020231007A1 (en) 2021-04-29
MX2021003777A (es) 2021-07-21
CN112969802A (zh) 2021-06-15
NL2023327B1 (en) 2020-09-17
IL281861A (en) 2021-05-31
EP3837384A1 (en) 2021-06-23
BR112021006428A2 (pt) 2021-09-21
JP2022522076A (ja) 2022-04-14

Similar Documents

Publication Publication Date Title
GB2600028B (en) Detection of phishing campaigns
EP4077722C0 (en) METHOD FOR DETECTING AN ANALYTE
EP3724317A4 (en) ANALYTE DETECTION
EP3902472A4 (en) ANALYZER SENSOR BREAKDOWN MITIGATION
EP4077717C0 (en) METHOD FOR DETECTING AN ANALYTE
IL281861A (en) Multiple fluorescence detection of analytes
EP3681504A4 (en) METHOD OF DETECTION USING X-RAY FLUORESCENCE
EP4041906A4 (en) HIGHLY MULTIPLEXED DETECTION OF NUCLEIC ACIDS
IL280772A (en) Multiplex testing of nucleic acids
EP3864165A4 (en) DETECTION OF AN ORIGINAL CANCER CELL
EP3803336A4 (en) DETECTION OF BIOLOGICAL SUBSTANCES
SG11202113179WA (en) Context detection
GB202114745D0 (en) Detection of speech
SG11202110398QA (en) Concurrent analysis of multiple analytes
GB201902630D0 (en) Analyte detection method
GB202002891D0 (en) Analyte detection
GB2591743B (en) Analyte detection system
GB201907550D0 (en) Detection of biomarkers
GB2596502B (en) Crypto-jacking detection
EP3977137A4 (en) DETECTION OF ANALYTES THROUGH NANOPORES WITHOUT THE USE OF ELECTRODES
GB201905861D0 (en) Methods of detecting cancer
EP3899531A4 (en) LUNG CANCER DETECTION METHOD
GB201906201D0 (en) Method for the detection of protate cancer
GB2603706B (en) Detection of LARP1
GB202016817D0 (en) Multiplex Detection of Biomarkers