SG11202009774XA - Compositions and methods for preparing nucleic acid libraries - Google Patents

Compositions and methods for preparing nucleic acid libraries

Info

Publication number
SG11202009774XA
SG11202009774XA SG11202009774XA SG11202009774XA SG11202009774XA SG 11202009774X A SG11202009774X A SG 11202009774XA SG 11202009774X A SG11202009774X A SG 11202009774XA SG 11202009774X A SG11202009774X A SG 11202009774XA SG 11202009774X A SG11202009774X A SG 11202009774XA
Authority
SG
Singapore
Prior art keywords
compositions
methods
nucleic acid
acid libraries
preparing nucleic
Prior art date
Application number
SG11202009774XA
Other languages
English (en)
Inventor
Zhihong Zhang
Tao Zheng
Bingsi Li
Wanglong Deng
Yusheng Han
Original Assignee
Guangzhou Burning Rock Dx Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangzhou Burning Rock Dx Co Ltd filed Critical Guangzhou Burning Rock Dx Co Ltd
Publication of SG11202009774XA publication Critical patent/SG11202009774XA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SG11202009774XA 2018-04-03 2019-04-02 Compositions and methods for preparing nucleic acid libraries SG11202009774XA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/CN2018/081748 WO2019191900A1 (en) 2018-04-03 2018-04-03 Compositions and methods for preparing nucleic acid libraries
PCT/CN2019/081059 WO2019192489A1 (en) 2018-04-03 2019-04-02 Compositions and methods for preparing nucleic acid libraries

Publications (1)

Publication Number Publication Date
SG11202009774XA true SG11202009774XA (en) 2020-10-29

Family

ID=68099745

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202009774XA SG11202009774XA (en) 2018-04-03 2019-04-02 Compositions and methods for preparing nucleic acid libraries

Country Status (9)

Country Link
US (2) US20210040475A1 (zh)
EP (1) EP3740604A4 (zh)
JP (1) JP2021517556A (zh)
CN (2) CN113106145A (zh)
AU (1) AU2019248276A1 (zh)
BR (1) BR112020020207A2 (zh)
CA (1) CA3095837A1 (zh)
SG (1) SG11202009774XA (zh)
WO (2) WO2019191900A1 (zh)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111455469B (zh) * 2020-04-07 2023-08-18 深圳易倍科华生物科技有限公司 一种单链快速建库方法及建库仪器
CN116829730A (zh) * 2020-11-10 2023-09-29 美国政府卫生与公众服务部 染色质占据和rna的单细胞分析测序
CN112538657B (zh) * 2020-12-25 2021-08-17 北京吉因加医学检验实验室有限公司 一种脑脊液基因测序建库、检测方法及其应用
CN113564226A (zh) * 2021-07-26 2021-10-29 深圳泰莱生物科技有限公司 一种捕获cfDNA5mC片段的检测方法
CN115896027A (zh) * 2022-04-07 2023-04-04 广州燃石医学检验所有限公司 一种生物组合物、其制备方法及应用
CN114736951A (zh) * 2022-04-20 2022-07-12 深圳大学 一种小分子rna的高通量测序文库构建方法
CN116287124A (zh) * 2023-05-24 2023-06-23 中国农业科学院农业基因组研究所 单链接头预连接方法、高通量测序文库的建库方法及试剂盒

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11201404243WA (en) * 2012-01-26 2014-08-28 Nugen Technologies Inc Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
JP6509723B2 (ja) * 2012-03-13 2019-05-08 スウィフト バイオサイエンシーズ， インコーポレイテッド 核酸ポリメラーゼによる基質ポリヌクレオチドの大きさ制御されたホモポリマーテーリングのための方法および組成物
WO2014071070A1 (en) * 2012-11-01 2014-05-08 Pacific Biosciences Of California, Inc. Compositions and methods for selection of nucleic acids
WO2014124338A1 (en) * 2013-02-08 2014-08-14 10X Technologies, Inc. Polynucleotide barcode generation
WO2014143157A1 (en) * 2013-03-13 2014-09-18 The Board Institute, Inc. Compositions and methods for long insert, paired end libraries of nucleic acids in emulsion droplets
GB201403216D0 (en) * 2014-02-24 2014-04-09 Cambridge Epigenetix Ltd Nucleic acid sample preparation
WO2015134552A1 (en) * 2014-03-03 2015-09-11 Swift Biosciences, Inc. Enhanced adaptor ligation
CN104264231B (zh) * 2014-09-30 2017-04-19 天津华大基因科技有限公司 构建测序文库的方法及其应用
CN106192021B (zh) * 2016-08-02 2017-04-26 中国海洋大学 一种串联rad标签测序文库的构建方法
CN106497920A (zh) * 2016-11-21 2017-03-15 深圳华大基因研究院 一种用于非小细胞肺癌基因突变检测的文库构建方法及试剂盒
EP3545106B1 (en) * 2017-08-01 2022-01-19 Helitec Limited Methods of enriching and determining target nucleotide sequences

Also Published As

Publication number Publication date
US20210040475A1 (en) 2021-02-11
EP3740604A4 (en) 2021-12-29
CN113106145A (zh) 2021-07-13
CA3095837A1 (en) 2019-10-10
JP2021517556A (ja) 2021-07-26
WO2019191900A1 (en) 2019-10-10
EP3740604A1 (en) 2020-11-25
US20210254051A1 (en) 2021-08-19
AU2019248276A1 (en) 2020-10-22
WO2019192489A1 (en) 2019-10-10
BR112020020207A2 (pt) 2021-01-19
CN110892097A (zh) 2020-03-17

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