SG11202004810UA - Multi-flux microfluidic chip for nucleic acid detection and capable of actively controlling flow path, and use method thereof - Google Patents

Multi-flux microfluidic chip for nucleic acid detection and capable of actively controlling flow path, and use method thereof

Info

Publication number
SG11202004810UA
SG11202004810UA SG11202004810UA SG11202004810UA SG11202004810UA SG 11202004810U A SG11202004810U A SG 11202004810UA SG 11202004810U A SG11202004810U A SG 11202004810UA SG 11202004810U A SG11202004810U A SG 11202004810UA SG 11202004810U A SG11202004810U A SG 11202004810UA
Authority
SG
Singapore
Prior art keywords
nucleic acid
flow path
use method
microfluidic chip
acid detection
Prior art date
Application number
SG11202004810UA
Inventor
Xingshang Xu
Jeffery Chen
Tao Zhu
Pei Yu
Original Assignee
Nanjing Lansion Biotechnology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanjing Lansion Biotechnology Co Ltd filed Critical Nanjing Lansion Biotechnology Co Ltd
Publication of SG11202004810UA publication Critical patent/SG11202004810UA/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B01L3/50825—Closing or opening means, corks, bungs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0825—Test strips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Clinical Laboratory Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
SG11202004810UA 2017-09-26 2017-12-06 Multi-flux microfluidic chip for nucleic acid detection and capable of actively controlling flow path, and use method thereof SG11202004810UA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201710878470.1A CN107739706B (en) 2017-09-26 2017-09-26 Multi-flux microfluidic nucleic acid detection chip for actively controlling flow path and application method thereof
PCT/CN2017/114693 WO2019061816A1 (en) 2017-09-26 2017-12-06 Multi-flux microfluidic nucleic acid detection chip for actively controlling flow path, and usage method thereof

Publications (1)

Publication Number Publication Date
SG11202004810UA true SG11202004810UA (en) 2020-06-29

Family

ID=61235286

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202004810UA SG11202004810UA (en) 2017-09-26 2017-12-06 Multi-flux microfluidic chip for nucleic acid detection and capable of actively controlling flow path, and use method thereof

Country Status (5)

Country Link
US (1) US10875019B2 (en)
EP (1) EP3489344B1 (en)
CN (1) CN107739706B (en)
SG (1) SG11202004810UA (en)
WO (1) WO2019061816A1 (en)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3774052A4 (en) * 2018-04-04 2022-01-05 Combinati Incorporated Microfluidic siphoning array for nucleic acid quantification
WO2019222872A1 (en) * 2018-05-21 2019-11-28 深圳华大生命科学研究院 High-throughput organs-on-a-chip, and preparation method therefor and application thereof
CN108489957A (en) * 2018-06-07 2018-09-04 江苏大学 A kind of Portable fluorescence detection device and method
CN108816300B (en) * 2018-07-02 2020-05-01 京东方科技集团股份有限公司 Microfluidic chip, functional device and manufacturing method thereof
CN110856824B (en) * 2018-08-22 2024-06-04 厦门大学 Microfluidic chip
CN108872081B (en) * 2018-09-04 2023-06-23 重庆科技学院 Multilayer microfluidic chip for detecting heavy metal ions
CN109100525B (en) * 2018-09-08 2023-07-28 重庆科技学院 Using method of paper-based microfluidic chip for multi-channel detection
CN109030369B (en) * 2018-09-08 2023-07-14 重庆科技学院 Multichannel micro-fluidic chip combined with cuvette
CN109351368B (en) * 2018-10-23 2021-04-30 深圳市博瑞生物科技有限公司 Micro-fluidic chip
CN109765391B (en) * 2019-03-14 2023-04-28 杭州霆科生物科技有限公司 Multi-index detection centrifugal test strip chip
CN110551622A (en) * 2019-09-18 2019-12-10 无锡百泰克生物技术有限公司 quick PCR reaction chip and quick fluorescence quantitative detector
MX2022003932A (en) * 2019-10-02 2022-04-25 Becton Dickinson Co Microfluidic cartridges for enhanced amplification of polynucleotide-containing samples.
US11367792B2 (en) 2019-11-01 2022-06-21 Hefei Xinsheng Optoelectronics Technology Co., Ltd Thin film transistor, fabricating method thereof, and display apparatus
KR20220132550A (en) * 2020-01-06 2022-09-30 비수, 잉크. Microfluidic systems, devices and methods
US11717823B2 (en) 2020-01-06 2023-08-08 Bisu, Inc. Microfluidic system, device and method
CN111458502A (en) * 2020-03-08 2020-07-28 北京化工大学 Microfluidic HIV urine detection device
CN111269827A (en) * 2020-03-22 2020-06-12 大连保税区金宝至电子有限公司 Stainless steel etching processing nucleic acid detection micro-channel control chip
CN111558403B (en) * 2020-04-21 2021-02-26 深圳市芯凯瑞生物科技有限公司 Microfluidic detection chip and detection method thereof
CN111748463A (en) * 2020-08-07 2020-10-09 天津智善生物科技有限公司 Amplification device applied to nucleic acid detection and detection method based on amplification device
CN112300924A (en) * 2020-08-25 2021-02-02 墨卓生物科技(上海)有限公司 Integrated PCR instrument and control method thereof
CN112592815B (en) * 2020-12-25 2022-05-24 浙江大学 Micro-fluidic chip for carrying out multiple microRNA detection and application
WO2022213630A1 (en) * 2021-04-08 2022-10-13 埃妥生物科技(杭州)有限公司 Sample extractor and sample extraction method
CN113881560A (en) * 2021-11-15 2022-01-04 深圳市研元生物科技有限公司 Nucleic acid detecting plate embedded in gene chip
CN114231408B (en) * 2021-12-31 2024-04-30 圣湘生物科技股份有限公司 Nucleic acid detection chip and nucleic acid detection method
CN114452911B (en) * 2022-01-25 2023-09-19 大连海事大学 Method and device for processing ultra-long nanowire and ultra-long nanowire heterojunction material
CN114507584A (en) * 2022-01-26 2022-05-17 深圳市锦隆生物科技有限公司 Liquid external driving device, method and equipment of biological micro-fluidic chip card box
CN114720225B (en) * 2022-03-17 2024-05-28 北京化工大学 Multi-index joint inspection device with microfluidic sample pretreatment
CN114733587B (en) * 2022-04-01 2024-02-20 合肥诺迈基生物科技有限公司 Fluorescence detection chip, fluorescence detection system, fluorescence detection method and application thereof
CN114931986B (en) * 2022-04-08 2022-12-27 厦门宝太生物科技股份有限公司 Nucleic acid detection micro-fluidic device and nucleic acid detection method thereof
CN115155678B (en) * 2022-06-09 2024-02-09 安图实验仪器(郑州)有限公司 Centrifugal microfluidic analysis chip
CN115475667B (en) * 2022-08-03 2024-07-16 南方科技大学 Microfluidic chip and storage system
CN117025375A (en) * 2023-04-03 2023-11-10 中国科学院苏州生物医学工程技术研究所 Microfluidic chip, microfluidic chip assembly and digital nucleic acid detection sampling device
CN116515617B (en) * 2023-07-03 2023-10-20 杭州杰毅生物技术有限公司 Centrifugal PCR-fluorescence multi-target detection chip
CN116532175B (en) * 2023-07-06 2023-10-13 北京中医药大学 Rolling type micro-fluidic chip and control method

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6720187B2 (en) * 2000-06-28 2004-04-13 3M Innovative Properties Company Multi-format sample processing devices
GB2414059B (en) * 2004-05-10 2008-06-11 E2V Tech Uk Ltd Microfluidic device
CN100518923C (en) * 2006-01-19 2009-07-29 财团法人工业技术研究院 Self-closing type high temperature bio-chemical reaction equipment, and method therefor
US7998708B2 (en) * 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US8337777B2 (en) * 2006-06-28 2012-12-25 Applied Biosystems, Llc Sample distribution devices and methods
GB2445738A (en) * 2007-01-16 2008-07-23 Lab901 Ltd Microfluidic device
KR101305976B1 (en) * 2007-02-12 2013-09-12 삼성전자주식회사 Centrifugal force based microfluidic device for serial dilution and microfluidic system comprising the same
KR20100128518A (en) * 2009-05-28 2010-12-08 경북대학교 산학협력단 Pcr chip using nanofluids and method for manufacuring pcr chip
US8720036B2 (en) * 2010-03-09 2014-05-13 Netbio, Inc. Unitary biochip providing sample-in to results-out processing and methods of manufacture
US9795968B2 (en) * 2014-04-21 2017-10-24 Lawrence Livermore National Security, LLCq Multi-chamber nucleic acid amplification and detection device
US10875024B2 (en) * 2014-07-10 2020-12-29 The Board Of Regents Of The University Of Texas System Methods and compositions for paper-based and hybrid microfluidic devices integrated with nucleic acid amplification for disease diagnosis
CN107225006B (en) * 2017-07-03 2018-04-03 南京岚煜生物科技有限公司 More flux micro-fluidic chips based on the flowing of active control liquid

Also Published As

Publication number Publication date
WO2019061816A1 (en) 2019-04-04
EP3489344B1 (en) 2021-05-19
EP3489344A1 (en) 2019-05-29
US10875019B2 (en) 2020-12-29
CN107739706A (en) 2018-02-27
CN107739706B (en) 2020-04-14
EP3489344A4 (en) 2019-07-03
US20190351412A1 (en) 2019-11-21

Similar Documents

Publication Publication Date Title
SG11202004810UA (en) Multi-flux microfluidic chip for nucleic acid detection and capable of actively controlling flow path, and use method thereof
SG11201913816TA (en) Multi-flux micro-fluidic chip based on active fluid flow control
IL246980A0 (en) Chip device for monitoring and regulating fluid flow, and methods of manufacture thereof
SG11201914032VA (en) Single-channel chemiluminescent micro-fluidic chip and detection method thereof
SG11201610707RA (en) Nucleic acid amplification device, nucleic acid amplification method, and chip for nucleic acid amplification
EP3234517A4 (en) Method and system for fluid flow rate measurement
GB2556793B (en) Downhole fluid flow control system and method having autonomous flow control
GB2541504B (en) Flow control system and method
SG10201913810WA (en) Flow Control And Processing Cartridge
PL3219611T3 (en) Active control flow system and method of cooling and providing active flow control
EP3499099A4 (en) Fluid handling device, fluid handling method, and flow path chip
GB201712953D0 (en) Systes and methods for thermally actuated flow control
BR112018005887A2 (en) fluid method and system.
BR112018005891A2 (en) fluid method and system.
GB201913007D0 (en) System and methodology for controlling fluid flow
EP3250504A4 (en) Microfluidic chip for coagulation sensing
DK3157444T3 (en) AUTOMATIC SYSTEM FOR CONTROL OF BLOOD FLOW AND VEHIC PRESSURE
EP3467515A4 (en) Fluid device, system, method of detecting specimen substance, and method of purifying specimen substance
EP3297572A4 (en) Urine flow system and method of use
EP3626811A4 (en) Chip, detection system, and gene sequencing method
GB2540084B (en) Assessment and control of drilling fluid conditioning system
EP3400445C0 (en) Method of identifying novel protein aggregation inhibitors based on chemical kinetics
EP3467514A4 (en) Fluid device, system, and method of detecting specimen substance
EP3467121A4 (en) Target biological molecule identification method, beads for target biological molecule identification use, set of beads, and target biological molecule identification device
GB2553002B (en) Assembly for control and/or measurement of fluid flow