SG11201808479VA - Live, attenuated alphavirus constructs and methods and uses thereof - Google Patents

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization MD 1111111110111111 11111 01011111011101011101111111111111110111111111110111111 International Bureau Yejd :::„, (10) International Publication Number (43) International Publication Date WO 2017/172698 Al 5 October 2017 (05.10.2017) IN I PO I P CT (51) (21) (22) (25) Filing Language: (26) Publication Language: (30) (71) (72) (74) International Patent Classification: (81) Designated States (unless otherwise indicated, for every A61K 39/12 (2006.01) Cl 2N 15/86 (2006.01) kind of national protection available): AE, AG, AL, AM, C12N 7/00 (2006.01) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, International Application Number: DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, PCT/US2017/024450 HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, International Filing Date: KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, 28 March 2017 (28.03.2017) MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, English RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, English TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. Priority Data: 62/316,264 31 March 2016 (31.03.2016) US (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, Applicant: TAKEDA VACCINES, INC. [US/US]; One GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, Takeda Parkway, Deerfield, IL 60015 (US). TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, Inventors: LIVENGOOD, Jill, Ann; 2348 Hampshire DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, Court, Fort Collins, CO 80526 (US). POWELL, Timothy, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, Duane; 5 Blue Jay Way, Maynard, MA 01754 (US). SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, Agents: HANSON, Roberta et al.; 2200 Wells Fargo Cen- GW, KM, ML, MR, NE, SN, TD, TG). ter, 90 South Seventh St., Minneapolis, MN 55402-3901 Published: (US). — with international search report (Art. 21(3)) [Continued on next page] = . = LIVE, ATTENUATED ALPHAVIRUS CONSTRUCTS AND METHODS AND USES THEREOF (57) : Embodiments herein relate to live, attenuated alphaviruses that are incapable Vero Growth Curve of replicating in mosquito cells and of transmis- sion by mosquito vectors. Other embodiments 1.E+09 -,-*--FMDV concern methods of generating live, attenuated alphaviruses, constructs thereof and uses of Title: (54) = = - _ _ = — 1.E+08 .4%--HC V these live, attenuated alphaviruses in immuno- = genic compositions. Other embodiments relate 1.E+07 -it- E V71 to pharmaceutical compositions including the 1.E+06 live, attenuated alphaviruses and methods for _, = = .4 TME V manufacturing these live, attenuated viruses. -X- E I .E+05 Yet other embodiments relate to uses of these C tn 1.E+04 -- --- - -.)-ii-FIRV compositions in kits for portable applications = such as vaccines against alphavirus infection U1.E+03 -4I-CHIK- and methods thereof. Emi . = — — 1.E+02 IRES --.- . -..181-25 1.E+01 r 1.E+00 mock 1 2 3 Day FIG. 3 1-1 pc 01 N N 1-1 N 1-1 0 ei O WO 2017/172698 Al MIDEDIMOM0101011101010MOINIHOHINIMEMOVOIMIE — before the expiration of the time limit for amending the — with sequence listing part of description (Rule 5.2(a)) claims and amendments (Rule to be republished 48.2(h)) in the event of receipt of