SG11201806557VA - Differentiation induction from human pluripotent stem cells into hypothalamic neurons - Google Patents
Differentiation induction from human pluripotent stem cells into hypothalamic neuronsInfo
- Publication number
- SG11201806557VA SG11201806557VA SG11201806557VA SG11201806557VA SG11201806557VA SG 11201806557V A SG11201806557V A SG 11201806557VA SG 11201806557V A SG11201806557V A SG 11201806557VA SG 11201806557V A SG11201806557V A SG 11201806557VA SG 11201806557V A SG11201806557V A SG 11201806557VA
- Authority
- SG
- Singapore
- Prior art keywords
- pluripotent stem
- human pluripotent
- stem cells
- suspension
- substance
- Prior art date
Links
- 230000002267 hypothalamic effect Effects 0.000 title abstract 6
- 210000001778 pluripotent stem cell Anatomy 0.000 title abstract 5
- 230000006698 induction Effects 0.000 title abstract 4
- 230000004069 differentiation Effects 0.000 title abstract 3
- 210000002569 neuron Anatomy 0.000 title abstract 3
- 239000000126 substance Substances 0.000 abstract 7
- 238000012258 culturing Methods 0.000 abstract 5
- 239000000725 suspension Substances 0.000 abstract 5
- 210000001519 tissue Anatomy 0.000 abstract 5
- 210000004027 cell Anatomy 0.000 abstract 4
- 238000000034 method Methods 0.000 abstract 4
- 230000008410 smoothened signaling pathway Effects 0.000 abstract 4
- 102000007350 Bone Morphogenetic Proteins Human genes 0.000 abstract 3
- 108010007726 Bone Morphogenetic Proteins Proteins 0.000 abstract 3
- 230000003213 activating effect Effects 0.000 abstract 3
- 229940112869 bone morphogenetic protein Drugs 0.000 abstract 3
- 210000003850 cellular structure Anatomy 0.000 abstract 3
- 230000001817 pituitary effect Effects 0.000 abstract 3
- 230000019491 signal transduction Effects 0.000 abstract 3
- 210000003016 hypothalamus Anatomy 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
- C12N5/0619—Neurons
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0613—Cells from endocrine organs
- C12N5/0616—Pituitary gland
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/02—Atmosphere, e.g. low oxygen conditions
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/13—Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/40—Regulators of development
- C12N2501/41—Hedgehog proteins; Cyclopamine (inhibitor)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
- C12N2501/72—Transferases [EC 2.]
- C12N2501/727—Kinases (EC 2.7.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/999—Small molecules not provided for elsewhere
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/07—Coculture with; Conditioned medium produced by endocrine cells
- C12N2502/076—Coculture with; Conditioned medium produced by endocrine cells pituitary cells
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- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/08—Coculture with; Conditioned medium produced by cells of the nervous system
- C12N2502/081—Coculture with; Conditioned medium produced by cells of the nervous system neurons
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/03—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from non-embryonic pluripotent stem cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/30—Synthetic polymers
- C12N2533/32—Polylysine, polyornithine
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/52—Fibronectin; Laminin
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Neurology (AREA)
- Cell Biology (AREA)
- Endocrinology (AREA)
- Neurosurgery (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Gastroenterology & Hepatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Materials For Medical Uses (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2016010940 | 2016-01-22 | ||
| PCT/JP2017/001544 WO2017126551A1 (ja) | 2016-01-22 | 2017-01-18 | ヒト多能性幹細胞から視床下部ニューロンへの分化誘導 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201806557VA true SG11201806557VA (en) | 2018-08-30 |
Family
ID=59362262
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201806557VA SG11201806557VA (en) | 2016-01-22 | 2017-01-18 | Differentiation induction from human pluripotent stem cells into hypothalamic neurons |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US20190010452A1 (de) |
| EP (1) | EP3406709A4 (de) |
| JP (2) | JP7023496B2 (de) |
| KR (1) | KR20180101467A (de) |
| CN (1) | CN108495929B (de) |
| AU (1) | AU2017209742B2 (de) |
| CA (1) | CA3013553A1 (de) |
| IL (1) | IL260380B2 (de) |
| MY (1) | MY194128A (de) |
| SG (1) | SG11201806557VA (de) |
| WO (1) | WO2017126551A1 (de) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3715450A4 (de) * | 2017-11-24 | 2021-08-25 | Sumitomo Chemical Company, Limited | Verfahren zur herstellung von zellmasse, einschliesslich hypophysengewebe, und zellmasse davon |
| JP7491533B2 (ja) | 2019-11-20 | 2024-05-28 | 住友ファーマ株式会社 | 神経系細胞の凍結方法 |
| CA3162273A1 (en) | 2019-11-20 | 2021-05-27 | Sumitomo Pharma Co., Ltd. | Method for freezing cell aggregates |
| CN113046306B (zh) * | 2021-03-12 | 2022-10-18 | 广东东阳光药业有限公司 | 一种多能干细胞的培养方法 |
| AU2022355700A1 (en) * | 2021-09-30 | 2024-05-02 | National University Corporation Tokai National Higher Education And Research System | Cell aggregate including pituitary hormone-producing cells and method for producing same |
| JPWO2023054396A1 (de) * | 2021-09-30 | 2023-04-06 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BRPI0408733A (pt) * | 2003-03-12 | 2006-03-07 | Reliance Life Sciences Pvt Ltd | derivação de neurÈnios dopaminérgicos terminalmente diferenciados de células tronco embriÈnicas humanas |
| ES2928319T3 (es) * | 2011-10-31 | 2022-11-17 | Riken | Método para el cultivo de células madre |
| CN105431522B (zh) | 2013-03-14 | 2021-08-03 | 加利福尼亚大学董事会 | 内侧神经节隆起前体细胞的体外产生 |
| US11198850B2 (en) * | 2013-11-22 | 2021-12-14 | Riken | Method for manufacturing telencephalon or progenitor tissue thereof |
| EP3954763A1 (de) * | 2014-07-25 | 2022-02-16 | Riken | Verfahren zur herstellung einer adenohypophyse oder einem vorläufergewebe davon |
-
2017
- 2017-01-18 AU AU2017209742A patent/AU2017209742B2/en active Active
- 2017-01-18 WO PCT/JP2017/001544 patent/WO2017126551A1/ja active Application Filing
- 2017-01-18 EP EP17741428.1A patent/EP3406709A4/de active Pending
- 2017-01-18 MY MYPI2018702549A patent/MY194128A/en unknown
- 2017-01-18 IL IL260380A patent/IL260380B2/en unknown
- 2017-01-18 JP JP2017562850A patent/JP7023496B2/ja active Active
- 2017-01-18 CA CA3013553A patent/CA3013553A1/en active Pending
- 2017-01-18 SG SG11201806557VA patent/SG11201806557VA/en unknown
- 2017-01-18 CN CN201780007313.1A patent/CN108495929B/zh active Active
- 2017-01-18 US US16/068,929 patent/US20190010452A1/en active Pending
- 2017-01-18 KR KR1020187022706A patent/KR20180101467A/ko not_active Application Discontinuation
-
2022
- 2022-02-02 JP JP2022014885A patent/JP7465569B2/ja active Active
Also Published As
| Publication number | Publication date |
|---|---|
| JP7465569B2 (ja) | 2024-04-11 |
| US20190010452A1 (en) | 2019-01-10 |
| AU2017209742B2 (en) | 2022-12-01 |
| JPWO2017126551A1 (ja) | 2018-11-22 |
| IL260380B2 (en) | 2023-09-01 |
| EP3406709A1 (de) | 2018-11-28 |
| EP3406709A4 (de) | 2019-08-28 |
| AU2017209742A1 (en) | 2018-08-23 |
| MY194128A (en) | 2022-11-14 |
| CA3013553A1 (en) | 2017-07-27 |
| JP7023496B2 (ja) | 2022-02-22 |
| IL260380A (de) | 2018-08-30 |
| KR20180101467A (ko) | 2018-09-12 |
| CN108495929A (zh) | 2018-09-04 |
| CN108495929B (zh) | 2023-04-18 |
| IL260380B1 (en) | 2023-05-01 |
| WO2017126551A1 (ja) | 2017-07-27 |
| JP2022062165A (ja) | 2022-04-19 |
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