SG11201806557VA - Differentiation induction from human pluripotent stem cells into hypothalamic neurons - Google Patents

Differentiation induction from human pluripotent stem cells into hypothalamic neurons

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Publication number
SG11201806557VA
SG11201806557VA SG11201806557VA SG11201806557VA SG11201806557VA SG 11201806557V A SG11201806557V A SG 11201806557VA SG 11201806557V A SG11201806557V A SG 11201806557VA SG 11201806557V A SG11201806557V A SG 11201806557VA SG 11201806557V A SG11201806557V A SG 11201806557VA
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Singapore
Prior art keywords
pluripotent stem
human pluripotent
stem cells
suspension
substance
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SG11201806557VA
Inventor
Hidetaka Suga
Koichiro Ogawa
Takatoshi Kasai
Hiroshi Arima
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Univ Nagoya Nat Univ Corp
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Publication date
Application filed by Univ Nagoya Nat Univ Corp filed Critical Univ Nagoya Nat Univ Corp
Publication of SG11201806557VA publication Critical patent/SG11201806557VA/en

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    • C12N2533/52—Fibronectin; Laminin

Abstract

DIFFERENTIATION INDUCTION FROM HUMAN PLURIPOTENT STEM CELLS INTO HYPOTHALAMIC NEURONS 5 There is provided a method for efficient differentiation induction from human pluripotent stem cells into hypothalamic neurons. Also, provided is a method for constructing, from human pluripotent stem cells, a cellular structure in which hypothalamic tissue and pituitary tissue are integrated. A cellular structure including hypothalamic tissue is obtained by a method including the steps of: culturing an aggregate of human pluripotent stem 10 cells in suspension in a medium containing a low concentration of a bone morphogenetic protein signal transduction pathway activating substance and a low concentration of a substance acting on the Shh signaling pathway; and further culturing the cell aggregate obtained in the step in suspension in a medium containing a low concentration of a substance acting on the Shh signaling pathway. Also, a cellular structure including hypothalamic tissue 15 and pituitary tissue is obtained by a method including the steps of: culturing an aggregate of human pluripotent stem cells in suspension in a medium containing a bone morphogenetic protein signal transduction pathway activating substance and a substance acting on the Shh signaling pathway; further culturing the cell aggregate formed in the step in suspension in a medium containing a bone morphogenetic protein signal transduction pathway activating 20 substance and a substance acting on the Shh signaling pathway; and culturing the cell aggregate obtained in the step in suspension in a medium suitable for simultaneous induction of pituitary and hypothalamus. [Figure 14] 25
SG11201806557VA 2016-01-22 2017-01-18 Differentiation induction from human pluripotent stem cells into hypothalamic neurons SG11201806557VA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2016010940 2016-01-22
PCT/JP2017/001544 WO2017126551A1 (en) 2016-01-22 2017-01-18 Differentiation induction from human pluripotent stem cells into hypothalamic neurons

Publications (1)

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SG11201806557VA true SG11201806557VA (en) 2018-08-30

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Country Status (11)

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US (1) US20190010452A1 (en)
EP (1) EP3406709A4 (en)
JP (2) JP7023496B2 (en)
KR (1) KR20180101467A (en)
CN (1) CN108495929B (en)
AU (1) AU2017209742B2 (en)
CA (1) CA3013553A1 (en)
IL (1) IL260380B2 (en)
MY (1) MY194128A (en)
SG (1) SG11201806557VA (en)
WO (1) WO2017126551A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111386336A (en) * 2017-11-24 2020-07-07 住友化学株式会社 Method for producing cell mass containing pituitary tissue and cell mass
CN115175989A (en) 2019-11-20 2022-10-11 住友制药株式会社 Method for freezing nerve cells
US20220408717A1 (en) 2019-11-20 2022-12-29 Sumitomo Pharma Co., Ltd. Method for freezing cell aggregates
CN113046306B (en) * 2021-03-12 2022-10-18 广东东阳光药业有限公司 Culture method of pluripotent stem cells
TW202330905A (en) * 2021-09-30 2023-08-01 日商住友製藥股份有限公司 Cell aggregate including pituitary hormone-producing cells and method for producing same
CA3234671A1 (en) * 2021-09-30 2023-04-06 Sumitomo Chemical Company, Limited Method for producing cell mass including pituitary tissue and cell mass

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1613726A4 (en) * 2003-03-12 2007-08-22 Reliance Life Sciences Pvt Ltd Derivation of terminally differentiated dopaminergic neurons from human embryonic stem cells
US10808224B2 (en) * 2011-10-31 2020-10-20 Riken Method for culturing stem cell
CN105431522B (en) * 2013-03-14 2021-08-03 加利福尼亚大学董事会 In vitro generation of medial ganglionic eminence precursor cells
MY188836A (en) 2013-11-22 2022-01-07 Sumitomo Chemical Co Method for manufacturing telencephalon or progenitor tissue thereof
JP6789814B2 (en) * 2014-07-25 2020-11-25 国立研究開発法人理化学研究所 Method for producing glandular pituitary gland or its precursor tissue

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JP7465569B2 (en) 2024-04-11
JPWO2017126551A1 (en) 2018-11-22
AU2017209742A1 (en) 2018-08-23
KR20180101467A (en) 2018-09-12
CA3013553A1 (en) 2017-07-27
CN108495929B (en) 2023-04-18
JP7023496B2 (en) 2022-02-22
JP2022062165A (en) 2022-04-19
WO2017126551A1 (en) 2017-07-27
EP3406709A4 (en) 2019-08-28
MY194128A (en) 2022-11-14
AU2017209742B2 (en) 2022-12-01
IL260380B1 (en) 2023-05-01
US20190010452A1 (en) 2019-01-10
IL260380A (en) 2018-08-30
CN108495929A (en) 2018-09-04
IL260380B2 (en) 2023-09-01
EP3406709A1 (en) 2018-11-28

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