SG11201804082XA - Improved protein separation in ion exchange chromatography - Google Patents

Improved protein separation in ion exchange chromatography

Info

Publication number
SG11201804082XA
SG11201804082XA SG11201804082XA SG11201804082XA SG11201804082XA SG 11201804082X A SG11201804082X A SG 11201804082XA SG 11201804082X A SG11201804082X A SG 11201804082XA SG 11201804082X A SG11201804082X A SG 11201804082XA SG 11201804082X A SG11201804082X A SG 11201804082XA
Authority
SG
Singapore
Prior art keywords
international
pct
preparative
protein separation
ion exchange
Prior art date
Application number
SG11201804082XA
Inventor
Matthias Joehnck
Original Assignee
Merck Patent Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent Gmbh filed Critical Merck Patent Gmbh
Publication of SG11201804082XA publication Critical patent/SG11201804082XA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property - Organization International Bureau (43) International Publication Date .... ....r .....1 26 May 2017(26.05.2017) WIPO I PCT (10) WO International 111111111111311111111111111111111111111111111111111111111111111111111111111111111111111111 2017/084737 Publication Number Al (51) International Patent Classification: BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, CO7K 1/16 (2006.01) C07K 16/00 (2006.01) DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, CO7K 1/18 (2006.01) HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, (21) International Application Number: MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, PCT/EP2016/001803 OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (22) International Filing Date: SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, 28 October 2016 (28.10.2016) TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (25) Filing Language: English (84) Designated States (unless otherwise indicated, for every (26) Publication Language: English kind of regional protection available): ARIPO (BW, GH, (30) Priority Data: GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, 15195242.1 18 November 2015 (18.11.2015) EP TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, (71) Applicant: MERCK PATENT GMBH [DE/DE]; Frank- DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, furter Strasse 250, 64293 Darmstadt (DE). LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, (72) Inventor: JOEHNCK, Matthias; Lindenbergweg 3, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, 64367 Muehltal (DE). GW, KM, ML, MR, NE, SN, TD, TG). (81) Designated States (unless otherwise indicated, for every Published: kind of national protection available): AE, AG, AL, AM, — with international search report (Art 21(3)) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, 1-1 .4t IN M IN i t Ge C::: , IN 11 0 (54) Title: IMPROVED PROTEIN SEPARATION IN ION EXCHANGE CHROMATOGRAPHY ei 0 (57) : The present invention relates to improved preparative (>5 g/1) protein separations. These improvements are achieved by combining salt and pH gradients for preparative protein separations in combination with the development of a preparative step elution protein separation based on data generated by combined salt-pH gradient runs.
SG11201804082XA 2015-11-18 2016-10-28 Improved protein separation in ion exchange chromatography SG11201804082XA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP15195242 2015-11-18
PCT/EP2016/001803 WO2017084737A1 (en) 2015-11-18 2016-10-28 Improved protein separation in ion exchange chromatography

Publications (1)

Publication Number Publication Date
SG11201804082XA true SG11201804082XA (en) 2018-06-28

Family

ID=54608366

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201804082XA SG11201804082XA (en) 2015-11-18 2016-10-28 Improved protein separation in ion exchange chromatography

Country Status (13)

Country Link
US (1) US20180327447A1 (en)
EP (1) EP3377513A1 (en)
JP (1) JP2018538268A (en)
KR (1) KR20180083409A (en)
CN (1) CN108350026A (en)
AU (1) AU2016355739A1 (en)
BR (1) BR112018009881A2 (en)
CA (1) CA3005483A1 (en)
IL (1) IL259180A (en)
MX (1) MX2018005834A (en)
RU (1) RU2018121653A (en)
SG (1) SG11201804082XA (en)
WO (1) WO2017084737A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20210253631A1 (en) * 2020-01-24 2021-08-19 Alkermes, Inc. Methods of purification
WO2021210662A1 (en) * 2020-04-17 2021-10-21 小野薬品工業株式会社 Color removal method for protein formulation active ingredient

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU760048B2 (en) * 1998-05-06 2003-05-08 Genentech Inc. Protein purification by ion exchange chromatography
CN1234725C (en) * 2004-04-07 2006-01-04 陈志南 High performance quick purifying method for preparing piecewise antibody
TWI391399B (en) * 2005-05-25 2013-04-01 Hoffmann La Roche Method for determining the concentration of a salt for eluting a polypeptide
AU2011234532B2 (en) * 2010-03-30 2015-04-09 Octapharma Ag Process for the purification of a Growth Factor Protein
SG10201701224UA (en) * 2012-03-12 2017-04-27 Merck Patent Gmbh Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode
WO2013158279A1 (en) * 2012-04-20 2013-10-24 Abbvie Inc. Protein purification methods to reduce acidic species
PL2895188T3 (en) * 2012-09-11 2018-06-29 Coherus Biosciences, Inc. Correctly folded etanercept in high purity and excellent yield
CN104628846B (en) * 2013-11-06 2019-12-06 三生国健药业（上海）股份有限公司 Method for purifying recombinant protein

Also Published As

Publication number Publication date
MX2018005834A (en) 2018-08-01
BR112018009881A2 (en) 2018-11-13
KR20180083409A (en) 2018-07-20
CA3005483A1 (en) 2017-05-26
US20180327447A1 (en) 2018-11-15
IL259180A (en) 2018-07-31
WO2017084737A1 (en) 2017-05-26
RU2018121653A3 (en) 2020-01-31
AU2016355739A1 (en) 2018-07-05
RU2018121653A (en) 2019-12-19
CN108350026A (en) 2018-07-31
EP3377513A1 (en) 2018-09-26
JP2018538268A (en) 2018-12-27

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