SG11201701655YA - Differentiation of hepatocyte-like cells from stem cells - Google Patents

Differentiation of hepatocyte-like cells from stem cells

Info

Publication number
SG11201701655YA
SG11201701655YA SG11201701655YA SG11201701655YA SG11201701655YA SG 11201701655Y A SG11201701655Y A SG 11201701655YA SG 11201701655Y A SG11201701655Y A SG 11201701655YA SG 11201701655Y A SG11201701655Y A SG 11201701655YA SG 11201701655Y A SG11201701655Y A SG 11201701655YA
Authority
SG
Singapore
Prior art keywords
cells
hepatocyte
differentiation
stem cells
stem
Prior art date
Application number
SG11201701655YA
Other languages
English (en)
Inventor
Hanry Yu
Derek Phan
Yi-Chin Toh
Farah Tasnim
Original Assignee
Agency Science Tech & Res
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency Science Tech & Res filed Critical Agency Science Tech & Res
Priority to SG11201701655YA priority Critical patent/SG11201701655YA/en
Publication of SG11201701655YA publication Critical patent/SG11201701655YA/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5067—Liver cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
    • C12N2500/62—DMSO
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/724—Glycosyltransferases (EC 2.4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/73—Hydrolases (EC 3.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Wood Science & Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SG11201701655YA 2014-09-19 2015-09-17 Differentiation of hepatocyte-like cells from stem cells SG11201701655YA (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SG11201701655YA SG11201701655YA (en) 2014-09-19 2015-09-17 Differentiation of hepatocyte-like cells from stem cells

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SG10201405916Y 2014-09-19
PCT/SG2015/050325 WO2016043666A1 (fr) 2014-09-19 2015-09-17 Différenciation de cellules de type hépatocytaire à partir de cellules souches
SG11201701655YA SG11201701655YA (en) 2014-09-19 2015-09-17 Differentiation of hepatocyte-like cells from stem cells

Publications (1)

Publication Number Publication Date
SG11201701655YA true SG11201701655YA (en) 2017-04-27

Family

ID=55533580

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201701655YA SG11201701655YA (en) 2014-09-19 2015-09-17 Differentiation of hepatocyte-like cells from stem cells

Country Status (5)

Country Link
US (1) US10323228B2 (fr)
EP (1) EP3194569A4 (fr)
CN (1) CN107075467B (fr)
SG (1) SG11201701655YA (fr)
WO (1) WO2016043666A1 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20180295834A1 (en) * 2017-04-06 2018-10-18 The University Of North Carolina At Chapel Hill Cryopreservation method
KR102015562B1 (ko) * 2017-12-04 2019-08-28 한국화학연구원 극성을 갖는 간세포 유사세포로의 분화를 유도하는 조성물 및 상기 조성물을 이용한 간세포 유사세포로의 분화 방법
CN108486037B (zh) * 2018-02-12 2022-03-15 中山大学附属第三医院（中山大学肝脏病医院） 一种利用小分子化合物诱导人多能干细胞分化为肝细胞的方法
CN117802031A (zh) * 2018-09-30 2024-04-02 中国科学院分子细胞科学卓越创新中心 肝细胞的体外扩增培养方法及应用
CN109722411B (zh) * 2019-03-01 2022-05-06 安徽大学 一种促进胚胎干细胞自我更新状态的小分子的应用方法
MX2022011245A (es) 2020-03-11 2023-01-11 Bit Bio Ltd Método de generación de células hepáticas.
US20230407264A1 (en) 2020-03-13 2023-12-21 Goliver Therapeutics Hepatic Stem-Like Cells for the Treatment and/or the Prevention of Liver Disorders
WO2023093808A1 (fr) * 2021-11-24 2023-06-01 南京昕瑞再生医药科技有限公司 Composé et procédé d'amplification d'hépatocytes

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2692325C (fr) 2001-12-07 2015-10-20 Geron Corporation Cellules d'ilots pancreatiques provenant de cellules souches embryonnaires humaines
US7790867B2 (en) * 2002-12-05 2010-09-07 Rosetta Genomics Inc. Vaccinia virus-related nucleic acids and microRNA
WO2006034873A1 (fr) 2004-09-29 2006-04-06 Cellartis Ab Generation de cellules hepatocytaires a partir de souches derivant de blastocytes humains (hbs)
US8178349B2 (en) * 2005-02-09 2012-05-15 Burnham Institute For Medical Research Homogeneous neural precursor cells
US8278105B2 (en) 2008-09-09 2012-10-02 University Of Southern California Induction, propagation and isolation of liver progenitor cells
US9057051B2 (en) * 2008-10-31 2015-06-16 Katholieke Universiteit Leuven Optimized methods for differentiation of cells into cells with hepatocyte progenitor phenotypes, cells produced by the methods, and methods of using the cells
US20100143313A1 (en) 2008-12-10 2010-06-10 The General Hospital Corporation Homogeneous differentiation of hepatocyte-like cells from embryonic stem cells
JP5897543B2 (ja) * 2010-03-22 2016-03-30 セルアーティス アーベー Ｗｎｔシグナル経路の調節による多能性細胞から肝細胞様細胞への分化誘導と成熟
WO2011158125A2 (fr) * 2010-06-17 2011-12-22 Katholieke Universiteit Leuven Procédé de différenciation de cellules en cellules étoilées du foie et en cellules endothéliales sinusoïdales du foie, cellules produites par ces procédés, et procédés d'utilisation des cellules
WO2013018851A1 (fr) * 2011-08-02 2013-02-07 独立行政法人国立がん研究センター Procédé d'induction de la différentiation hépatique à partir d'une cellule souche hépatique induite et d'une cellule progénitrice hépatique induite
WO2013062140A1 (fr) * 2011-10-28 2013-05-02 Kyoto University Procédé pour l'induction efficace de la différenciation de cellules souches pluripotentes en des cellules du lignage hépatique
JP6494515B2 (ja) * 2012-10-19 2019-04-03 エージェンシー フォー サイエンス， テクノロジー アンド リサーチ 幹細胞を１又は２以上の細胞系列に分化させる方法
WO2014124527A1 (fr) 2013-02-18 2014-08-21 University Health Network Procédés pour générer des hépatocytes et des cholangiocytes à partir de cellules souches pluripotentes
US9512406B2 (en) 2013-12-20 2016-12-06 The J. David Gladstone Institute, a testamentary trust established under the Will of J. David Gladstone Generating hepatocytes

Also Published As

Publication number Publication date
CN107075467A (zh) 2017-08-18
EP3194569A1 (fr) 2017-07-26
CN107075467B (zh) 2020-08-21
WO2016043666A1 (fr) 2016-03-24
EP3194569A4 (fr) 2018-03-07
US20170260503A1 (en) 2017-09-14
US10323228B2 (en) 2019-06-18

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