SG11201609055RA - Integrated single cell sequencing - Google Patents

Integrated single cell sequencing

Info

Publication number
SG11201609055RA
SG11201609055RA SG11201609055RA SG11201609055RA SG11201609055RA SG 11201609055R A SG11201609055R A SG 11201609055RA SG 11201609055R A SG11201609055R A SG 11201609055RA SG 11201609055R A SG11201609055R A SG 11201609055RA SG 11201609055R A SG11201609055R A SG 11201609055RA
Authority
SG
Singapore
Prior art keywords
single cell
integrated single
cell sequencing
sequencing
integrated
Prior art date
Application number
SG11201609055RA
Other languages
English (en)
Inventor
Jason A A West
Brian Fowler
Tze-Howe Charn
Christian F Johnson
Marc A Unger
Original Assignee
Fluidigm Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fluidigm Corp filed Critical Fluidigm Corp
Publication of SG11201609055RA publication Critical patent/SG11201609055RA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
SG11201609055RA 2014-05-08 2015-05-08 Integrated single cell sequencing SG11201609055RA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461990598P 2014-05-08 2014-05-08
US201462079495P 2014-11-13 2014-11-13
PCT/US2015/029986 WO2015172080A1 (en) 2014-05-08 2015-05-08 Integrated single cell sequencing

Publications (1)

Publication Number Publication Date
SG11201609055RA true SG11201609055RA (en) 2016-11-29

Family

ID=54393068

Family Applications (2)

Application Number Title Priority Date Filing Date
SG11201609055RA SG11201609055RA (en) 2014-05-08 2015-05-08 Integrated single cell sequencing
SG10202004286UA SG10202004286UA (en) 2014-05-08 2015-05-08 Integrated single cell sequencing

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG10202004286UA SG10202004286UA (en) 2014-05-08 2015-05-08 Integrated single cell sequencing

Country Status (6)

Country Link
US (2) US20150337295A1 (enrdf_load_stackoverflow)
EP (1) EP3140428B1 (enrdf_load_stackoverflow)
JP (1) JP6716465B2 (enrdf_load_stackoverflow)
CN (1) CN106460051A (enrdf_load_stackoverflow)
SG (2) SG11201609055RA (enrdf_load_stackoverflow)
WO (1) WO2015172080A1 (enrdf_load_stackoverflow)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106811460B (zh) * 2015-11-30 2020-11-27 浙江安诺优达生物科技有限公司 用于低频突变检测的二代测序文库的构建方法及试剂盒
EP3467121A4 (en) * 2016-05-25 2020-02-12 Nikon Corporation METHOD FOR IDENTIFYING TARGET BIOLOGICAL, BALLS FOR USE IN IDENTIFYING TARGET BIOLOGICAL, BALL SET, AND DEVICE FOR IDENTIFYING TARGET BIOLOGICAL
AU2019247841B2 (en) * 2018-04-04 2023-02-09 Nautilus Subsidiary, Inc. Methods of generating nanoarrays and microarrays
US10842821B2 (en) 2018-04-05 2020-11-24 Medeor Therapeutics, Inc. Cellular compositions derived from prior organ donors and methods of manufacture and use thereof
US10881692B2 (en) 2018-04-05 2021-01-05 Medeor Therapeutics, Inc. Compositions for establishing mixed chimerism and methods of manufacture thereof
MX2021003768A (es) * 2018-10-25 2021-05-27 Illumina Inc Metodos y composiciones para identificar ligandos en arreglos usando indices y codigos de barra.
JP7687954B2 (ja) * 2019-01-29 2025-06-03 モレキュラー アッセンブリーズ， インコーポレイテッド 核酸を合成するための再使用可能な開始剤
WO2023102141A2 (en) * 2021-12-02 2023-06-08 Bio-Rad Laboratories, Inc. Use of dna:rna duplex fragmentation

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9620769D0 (en) * 1996-10-04 1996-11-20 Brax Genomics Ltd Nucleic acid sequencing
ES2563643T3 (es) * 1997-04-01 2016-03-15 Illumina Cambridge Limited Método de secuenciación de ácido nucleico
GB9714716D0 (en) 1997-07-11 1997-09-17 Brax Genomics Ltd Characterising nucleic acids
US20060275782A1 (en) * 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
ES2400302T3 (es) * 2000-04-17 2013-04-09 Dyax Corp. Nuevos métodos para construir bibliotecas de paquetes génicos que presentan de forma colectiva los miembros de una familia diversa de péptidos, polipéptidos o proteínas
AUPQ974900A0 (en) * 2000-08-29 2000-09-21 Macquarie Research Limited Degenerate oligonucleotide gene-shuffling
EP1353935A4 (en) * 2000-12-07 2005-02-23 Penn State Res Found SELECTION OF TARGET CATALYTIC NUCLEIC ACIDS ON INFECTIOUS AGENTS
JP2004097158A (ja) * 2002-09-12 2004-04-02 Kureha Chem Ind Co Ltd 発現遺伝子同定用ｃＤＮＡタグの作成方法、及び該ｃＤＮＡタグを用いる遺伝子発現解析方法
EP4105644A3 (en) * 2006-03-31 2022-12-28 Illumina, Inc. Systems and devices for sequence by synthesis analysis
WO2009124255A2 (en) 2008-04-04 2009-10-08 Helicos Biosciences Corporation Methods for transcript analysis
US20100035249A1 (en) * 2008-08-05 2010-02-11 Kabushiki Kaisha Dnaform Rna sequencing and analysis using solid support
US9309566B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid amplification
US9625454B2 (en) * 2009-09-04 2017-04-18 The Research Foundation For The State University Of New York Rapid and continuous analyte processing in droplet microfluidic devices
EP3147374B1 (en) 2010-12-17 2019-01-30 Life Technologies Corporation Methods for nucleic acid amplification
CN112592960B (zh) * 2011-05-20 2024-08-27 富鲁达公司 核酸编码反应
CA2878787A1 (en) 2012-02-29 2013-09-06 Fluidigm Corporation Methods, systems, and devices for multiple single-cell capturing and processing using microfluidics
NO2694769T3 (enrdf_load_stackoverflow) 2012-03-06 2018-03-03
CA2895638A1 (en) 2013-03-15 2014-09-18 Fluidigm Corporation Methods and devices for analysis of defined multicellular combinations

Also Published As

Publication number Publication date
EP3140428B1 (en) 2021-07-28
EP3140428A1 (en) 2017-03-15
CN106460051A (zh) 2017-02-22
SG10202004286UA (en) 2020-06-29
EP3140428A4 (en) 2018-02-28
US20190249238A1 (en) 2019-08-15
JP6716465B2 (ja) 2020-07-01
JP2017514478A (ja) 2017-06-08
WO2015172080A1 (en) 2015-11-12
US20150337295A1 (en) 2015-11-26

Similar Documents

Publication Publication Date Title
IL274903A (en) cell
ZA201707334B (en) Cell
ZA201701823B (en) T cell receptors
GB201507368D0 (en) Cell
GB201503500D0 (en) Cell
GB201518817D0 (en) Cell
GB201514874D0 (en) Cell
GB201522097D0 (en) Cells
PL3509156T3 (pl) Roztwór elektrolitu
PL3134724T3 (pl) Komórka elektrochemiczna
SG11201610831PA (en) Lithium-sulphur battery
SG11201609055RA (en) Integrated single cell sequencing
GB201411985D0 (en) Battery
HUE042287T2 (hu) Lítium-kén cella
GB201416211D0 (en) Sequencing techniques
GB201409785D0 (en) Sequencing Techniques
GB201414968D0 (en) Cell assay
GB201410934D0 (en) T cell
GB201416750D0 (en) Cell preparation
GB201507819D0 (en) Cell
GB201507816D0 (en) Cell
GB201507814D0 (en) Cell
GB201507812D0 (en) Cell
GB201417228D0 (en) Batterys
GB201403494D0 (en) Identifying cell features