SG11201604907SA - Capturing specific nucleic acid materials from individual biological cells in a micro-fluidic device - Google Patents
Capturing specific nucleic acid materials from individual biological cells in a micro-fluidic deviceInfo
- Publication number
- SG11201604907SA SG11201604907SA SG11201604907SA SG11201604907SA SG11201604907SA SG 11201604907S A SG11201604907S A SG 11201604907SA SG 11201604907S A SG11201604907S A SG 11201604907SA SG 11201604907S A SG11201604907S A SG 11201604907SA SG 11201604907S A SG11201604907S A SG 11201604907SA
- Authority
- SG
- Singapore
- Prior art keywords
- micro
- nucleic acid
- specific nucleic
- fluidic device
- biological cells
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C5/00—Separating dispersed particles from liquids by electrostatic effect
- B03C5/005—Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C5/00—Separating dispersed particles from liquids by electrostatic effect
- B03C5/02—Separators
- B03C5/022—Non-uniform field separators
- B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0668—Trapping microscopic beads
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
- B01L2400/0424—Dielectrophoretic forces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0454—Moving fluids with specific forces or mechanical means specific forces radiation pressure, optical tweezers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C2201/00—Details of magnetic or electrostatic separation
- B03C2201/26—Details of magnetic or electrostatic separation for use in medical applications
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Dispersion Chemistry (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Clinical Laboratory Science (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Microelectronics & Electronic Packaging (AREA)
- Electrochemistry (AREA)
- Fluid Mechanics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicinal Preparation (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Computer Hardware Design (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US14/133,361 US20150166326A1 (en) | 2013-12-18 | 2013-12-18 | Capturing Specific Nucleic Acid Materials From Individual Biological Cells In A Micro-Fluidic Device |
PCT/US2014/071323 WO2015095623A1 (en) | 2013-12-18 | 2014-12-18 | Capturing specific nucleic acid materials from individual biological cells in a micro-fluidic device |
Publications (1)
Publication Number | Publication Date |
---|---|
SG11201604907SA true SG11201604907SA (en) | 2016-07-28 |
Family
ID=53367562
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG10201902353UA SG10201902353UA (en) | 2013-12-18 | 2014-12-18 | Capturing specific nucleic acid materials from individual biological cells in a micro-fluidic device |
SG11201604907SA SG11201604907SA (en) | 2013-12-18 | 2014-12-18 | Capturing specific nucleic acid materials from individual biological cells in a micro-fluidic device |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG10201902353UA SG10201902353UA (en) | 2013-12-18 | 2014-12-18 | Capturing specific nucleic acid materials from individual biological cells in a micro-fluidic device |
Country Status (7)
Country | Link |
---|---|
US (1) | US20150166326A1 (de) |
EP (1) | EP3083980B1 (de) |
JP (2) | JP6603663B2 (de) |
KR (2) | KR102448505B1 (de) |
DK (1) | DK3083980T3 (de) |
SG (2) | SG10201902353UA (de) |
WO (1) | WO2015095623A1 (de) |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9403172B2 (en) * | 2012-11-08 | 2016-08-02 | Berkeley Lights, Inc. | Circuit based optoelectronic tweezers |
US20150166326A1 (en) * | 2013-12-18 | 2015-06-18 | Berkeley Lights, Inc. | Capturing Specific Nucleic Acid Materials From Individual Biological Cells In A Micro-Fluidic Device |
WO2016172623A1 (en) | 2015-04-22 | 2016-10-27 | Berkeley Lights, Inc. | Manipulation of cell nuclei in a micro-fluidic device |
IL299366A (en) | 2015-11-23 | 2023-02-01 | Berkeley Lights Inc | Structures for microfluidic isolation are produced at the place of assembly, kits and their uses |
WO2017117521A1 (en) | 2015-12-31 | 2017-07-06 | Berkeley Lights, Inc. | Tumor infilitrating cells engineered to express a pro-inflammatory polypeptide |
CN109922885B (zh) | 2016-03-16 | 2022-05-10 | 伯克利之光生命科技公司 | 用于基因组编辑克隆的选择和传代的方法、系统和装置 |
CN109311930B (zh) * | 2016-03-31 | 2023-02-24 | 伯克利之光生命科技公司 | 核酸稳定试剂、试剂盒及其使用方法 |
IL267008B1 (en) | 2016-12-01 | 2024-02-01 | Berkeley Lights Inc | Imaging mechanisms and systems and methods for micro objects |
CN110268414B (zh) * | 2016-12-01 | 2023-08-01 | 伯克利之光生命科技公司 | 微流体装置中的微物体的自动检测和重新定位 |
EP3551745A4 (de) | 2016-12-12 | 2020-07-15 | Xcella Biosciences, Inc. | Verfahren und systeme zum screening unter verwendung von mikrokapillaren arrays |
AU2017388874A1 (en) | 2016-12-30 | 2019-07-18 | Berkeley Lights, Inc. | Methods for selection and generation of genome edited T cells |
EP3623461A4 (de) * | 2017-05-12 | 2021-01-20 | Nikon Corporation | Objektmanipulationsvorrichtung und objektmanipulationsverfahren |
CN110719956A (zh) | 2017-06-06 | 2020-01-21 | 齐默尔根公司 | 用于改良真菌菌株的高通量基因组工程改造平台 |
US11028401B2 (en) | 2018-06-06 | 2021-06-08 | Zymergen Inc. | Manipulation of genes involved in signal transduction to control fungal morphology during fermentation and production |
SG11202104544WA (en) * | 2018-11-19 | 2021-06-29 | Berkeley Lights Inc | Microfluidic device with programmable switching elements |
US11479779B2 (en) | 2020-07-31 | 2022-10-25 | Zymergen Inc. | Systems and methods for high-throughput automated strain generation for non-sporulating fungi |
Family Cites Families (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6685812B2 (en) * | 2001-01-09 | 2004-02-03 | The Regents Of The University Of California | Movement of particles using sequentially activated dielectrophoretic particle trapping |
US7312085B2 (en) * | 2002-04-01 | 2007-12-25 | Fluidigm Corporation | Microfluidic particle-analysis systems |
US6958132B2 (en) | 2002-05-31 | 2005-10-25 | The Regents Of The University Of California | Systems and methods for optical actuation of microfluidics based on opto-electrowetting |
JP5695287B2 (ja) * | 2002-10-02 | 2015-04-01 | カリフォルニア インスティテュート オブ テクノロジー | 微小流体の核酸解析 |
WO2004065618A2 (en) * | 2003-01-16 | 2004-08-05 | Thermogenic Imaging | Methods and devices for monitoring cellular metabolism in microfluidic cell-retaining chambers |
US20050142565A1 (en) | 2003-12-30 | 2005-06-30 | Agency For Science, Technology And Research | Nucleic acid purification chip |
US7612355B2 (en) | 2004-04-12 | 2009-11-03 | The Regents Of The University Of California | Optoelectronic tweezers for microparticle and cell manipulation |
WO2007008609A2 (en) * | 2005-07-07 | 2007-01-18 | The Regents Of The University Of California | Methods and apparatus for cell culture array |
EP1951742A4 (de) * | 2005-10-27 | 2011-06-01 | Life Technologies Corp | Optoelektronische trennung von biomolekülen |
EP1979079A4 (de) * | 2006-02-03 | 2012-11-28 | Integenx Inc | Mikrofluidische vorrichtungen |
EP2001990B1 (de) * | 2006-03-24 | 2016-06-29 | Handylab, Inc. | Integriertes system zur verarbeitung von mikrofluidischen proben und verwendungsverfahren |
US7439014B2 (en) * | 2006-04-18 | 2008-10-21 | Advanced Liquid Logic, Inc. | Droplet-based surface modification and washing |
WO2008119066A1 (en) | 2007-03-28 | 2008-10-02 | The Regents Of The University Of California | Single-sided lateral-field and phototransistor-based optoelectronic tweezers |
KR101228308B1 (ko) * | 2007-05-23 | 2013-01-31 | 삼성전자주식회사 | 미세유동 칩을 이용한 디스크형 미세유동장치 및 생체물질마이크로어레이 칩을 이용한 디스크형 미세유동장치 |
WO2008147530A1 (en) * | 2007-05-24 | 2008-12-04 | The Regents Of The University Of California | Integrated fluidics devices with magnetic sorting |
US9533306B2 (en) | 2010-08-02 | 2017-01-03 | The Regents Of The University Of California | Single sided continuous optoelectrowetting (SCEOW) device for droplet manipulation with light patterns |
EP2606154B1 (de) * | 2010-08-20 | 2019-09-25 | Integenx Inc. | Integriertes analysesystem |
EP2820394B1 (de) * | 2012-02-29 | 2017-07-12 | Fluidigm Corporation | Verfahren, systeme und vorrichtungen zur erfassung und verarbeitung mehrerer einzelzellen durch mikrofluidik |
US9857333B2 (en) | 2012-10-31 | 2018-01-02 | Berkeley Lights, Inc. | Pens for biological micro-objects |
US9403172B2 (en) | 2012-11-08 | 2016-08-02 | Berkeley Lights, Inc. | Circuit based optoelectronic tweezers |
US20150166326A1 (en) * | 2013-12-18 | 2015-06-18 | Berkeley Lights, Inc. | Capturing Specific Nucleic Acid Materials From Individual Biological Cells In A Micro-Fluidic Device |
-
2013
- 2013-12-18 US US14/133,361 patent/US20150166326A1/en not_active Abandoned
-
2014
- 2014-12-18 EP EP14872873.6A patent/EP3083980B1/de active Active
- 2014-12-18 SG SG10201902353UA patent/SG10201902353UA/en unknown
- 2014-12-18 KR KR1020217004925A patent/KR102448505B1/ko active IP Right Grant
- 2014-12-18 SG SG11201604907SA patent/SG11201604907SA/en unknown
- 2014-12-18 DK DK14872873.6T patent/DK3083980T3/da active
- 2014-12-18 KR KR1020167018502A patent/KR102220001B1/ko active IP Right Grant
- 2014-12-18 WO PCT/US2014/071323 patent/WO2015095623A1/en active Application Filing
- 2014-12-18 JP JP2016540965A patent/JP6603663B2/ja active Active
-
2019
- 2019-10-11 JP JP2019188011A patent/JP6954972B2/ja active Active
Also Published As
Publication number | Publication date |
---|---|
KR102220001B1 (ko) | 2021-02-25 |
SG10201902353UA (en) | 2019-04-29 |
US20150166326A1 (en) | 2015-06-18 |
EP3083980A4 (de) | 2017-08-30 |
JP2020022479A (ja) | 2020-02-13 |
EP3083980B1 (de) | 2021-10-13 |
WO2015095623A1 (en) | 2015-06-25 |
JP2017504315A (ja) | 2017-02-09 |
KR20210022146A (ko) | 2021-03-02 |
JP6954972B2 (ja) | 2021-10-27 |
KR20160098341A (ko) | 2016-08-18 |
DK3083980T3 (da) | 2021-12-13 |
EP3083980A1 (de) | 2016-10-26 |
KR102448505B1 (ko) | 2022-09-27 |
JP6603663B2 (ja) | 2019-11-06 |
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