SG11201601890UA - Application of oligo-dt molecules to avoid generation of high molecular pcr products induced by polya-carrier - Google Patents

Application of oligo-dt molecules to avoid generation of high molecular pcr products induced by polya-carrier

Info

Publication number
SG11201601890UA
SG11201601890UA SG11201601890UA SG11201601890UA SG11201601890UA SG 11201601890U A SG11201601890U A SG 11201601890UA SG 11201601890U A SG11201601890U A SG 11201601890UA SG 11201601890U A SG11201601890U A SG 11201601890UA SG 11201601890U A SG11201601890U A SG 11201601890UA
Authority
SG
Singapore
Prior art keywords
polya
oligo
molecules
carrier
application
Prior art date
Application number
SG11201601890UA
Other languages
English (en)
Inventor
Frank Bergmann
Stefanie Froehner
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of SG11201601890UA publication Critical patent/SG11201601890UA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SG11201601890UA 2013-09-13 2014-09-10 Application of oligo-dt molecules to avoid generation of high molecular pcr products induced by polya-carrier SG11201601890UA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP13184307 2013-09-13
PCT/EP2014/069294 WO2015036434A1 (fr) 2013-09-13 2014-09-10 Application de molécules oligo-dt pour éviter la production de produits de pcr de masse moléculaire élevée induite par un transporteur de polya

Publications (1)

Publication Number Publication Date
SG11201601890UA true SG11201601890UA (en) 2016-04-28

Family

ID=49165596

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201601890UA SG11201601890UA (en) 2013-09-13 2014-09-10 Application of oligo-dt molecules to avoid generation of high molecular pcr products induced by polya-carrier

Country Status (9)

Country Link
US (1) US9611505B2 (fr)
EP (1) EP3044326B1 (fr)
JP (1) JP6556728B2 (fr)
CN (1) CN105531381B (fr)
CA (1) CA2923083C (fr)
ES (1) ES2657988T3 (fr)
HK (1) HK1219301A1 (fr)
SG (1) SG11201601890UA (fr)
WO (1) WO2015036434A1 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6338221B2 (ja) 2012-10-24 2018-06-06 タカラ バイオ ユーエスエー, インコーポレイテッド 核酸生成物を生成するための、テンプレートスイッチに基づく方法
CN105658815B (zh) 2013-10-17 2020-12-29 塔卡拉生物美国有限公司 用于添加适配体至核酸的方法及用于实施所述方法的组合物
WO2015094861A1 (fr) 2013-12-17 2015-06-25 Clontech Laboratories, Inc. Procédés d'ajout d'adaptateurs à des acides nucléiques et compositions pour leur mise en œuvre
GB201805676D0 (en) * 2018-04-05 2018-05-23 Imperial Innovations Ltd Compositions

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0710296A4 (fr) * 1993-07-23 1999-05-26 Bio Rad Laboratories Amplification lineaire enchainee d'acides nucleiques
WO1996001835A1 (fr) * 1994-07-07 1996-01-25 Geron Corporation Telomerase mammifere
JP4194664B2 (ja) * 1997-02-26 2008-12-10 ザ ジェネラル ホスピタル コーポレイション アルツハイマー病の処置または予防のために効果的な薬物をスクリーニングするためのトランスジェニック動物および細胞株
CA2299119C (fr) * 1999-02-23 2013-02-05 Qiagen Gmbh Une methode pour stabiliser et/ou isoler des acides nucleiques
JP2007501005A (ja) * 2003-08-01 2007-01-25 インテグレーティッド ディーエヌエイ テクノロジーズ インコーポレーティッド ポリマー核酸を増幅するための方法
GB0818609D0 (en) * 2008-10-10 2008-11-19 Univ Hull apparatus and method
US8618253B2 (en) * 2010-05-25 2013-12-31 Samsung Techwin Co., Ltd. Modified RNAse H and detection of nucleic acid amplification
US10767214B2 (en) * 2010-10-28 2020-09-08 Clinical Genomics Pty Ltd Method of microvesicle enrichment
KR101870311B1 (ko) * 2012-03-09 2018-06-25 (주)바이오니아 핫스타트 역전사반응 또는 핫스타트 역전사 중합효소 연쇄반응용 조성물
SG11201405669XA (en) * 2012-03-13 2014-10-30 Swift Biosciences Inc Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase

Also Published As

Publication number Publication date
WO2015036434A1 (fr) 2015-03-19
CN105531381B (zh) 2019-08-09
HK1219301A1 (zh) 2017-03-31
CN105531381A (zh) 2016-04-27
ES2657988T3 (es) 2018-03-07
CA2923083C (fr) 2018-07-17
EP3044326A1 (fr) 2016-07-20
US9611505B2 (en) 2017-04-04
US20150079600A1 (en) 2015-03-19
EP3044326B1 (fr) 2017-12-06
JP2016529914A (ja) 2016-09-29
CA2923083A1 (fr) 2015-03-19
JP6556728B2 (ja) 2019-08-07

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