SG11201504584VA - Peg-mediated assembly of nucleic acid molecules - Google Patents

Peg-mediated assembly of nucleic acid molecules

Info

Publication number
SG11201504584VA
SG11201504584VA SG11201504584VA SG11201504584VA SG11201504584VA SG 11201504584V A SG11201504584V A SG 11201504584VA SG 11201504584V A SG11201504584V A SG 11201504584VA SG 11201504584V A SG11201504584V A SG 11201504584VA SG 11201504584V A SG11201504584V A SG 11201504584VA
Authority
SG
Singapore
Prior art keywords
peg
nucleic acid
acid molecules
mediated assembly
mediated
Prior art date
Application number
SG11201504584VA
Other languages
English (en)
Inventor
Jun Urano
Daniel G Gibson
Nicky C Caiazza
Zhiqing Qi
Original Assignee
Synthetic Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Synthetic Genomics Inc filed Critical Synthetic Genomics Inc
Publication of SG11201504584VA publication Critical patent/SG11201504584VA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • C12N15/1027Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • C12N15/1031Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
SG11201504584VA 2012-12-13 2013-12-11 Peg-mediated assembly of nucleic acid molecules SG11201504584VA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261736946P 2012-12-13 2012-12-13
PCT/US2013/074471 WO2014093535A1 (en) 2012-12-13 2013-12-11 Peg-mediated assembly of nucleic acid molecules

Publications (1)

Publication Number Publication Date
SG11201504584VA true SG11201504584VA (en) 2015-07-30

Family

ID=50934928

Family Applications (2)

Application Number Title Priority Date Filing Date
SG11201504584VA SG11201504584VA (en) 2012-12-13 2013-12-11 Peg-mediated assembly of nucleic acid molecules
SG10201704426UA SG10201704426UA (en) 2012-12-13 2013-12-11 Peg-mediated assembly of nucleic acid molecules

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG10201704426UA SG10201704426UA (en) 2012-12-13 2013-12-11 Peg-mediated assembly of nucleic acid molecules

Country Status (10)

Country Link
US (2) US20140308710A1 (ja)
EP (1) EP2931913B1 (ja)
JP (3) JP2015536689A (ja)
CN (2) CN109913519B (ja)
AU (1) AU2013359293B2 (ja)
CA (1) CA2893817C (ja)
DK (1) DK2931913T3 (ja)
IL (1) IL238918B (ja)
SG (2) SG11201504584VA (ja)
WO (1) WO2014093535A1 (ja)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2643356A1 (en) 2005-12-06 2008-02-28 J. Craig Venter Institute Synthetic genomes
US20140308710A1 (en) * 2012-12-13 2014-10-16 Synthetic Genomics, Inc. Peg-mediated assembly of nucleic acid molecules
WO2019044820A1 (ja) * 2017-08-28 2019-03-07 Spiber株式会社 2本鎖dna断片を製造する方法
JP2019198236A (ja) * 2018-05-14 2019-11-21 国立大学法人神戸大学 二本鎖dna合成方法
KR20210088615A (ko) * 2018-10-31 2021-07-14 지머젠 인코포레이티드 Dna 라이브러리의 다중 결정적 어셈블리

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5928905A (en) * 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US5834252A (en) * 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US7399590B2 (en) * 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US20090305233A1 (en) * 2007-07-03 2009-12-10 Arizona Board Of Regents, A Body Corporate Of The State Of Arizona Methods and Reagents for Polynucleotide Assembly
JP2009284896A (ja) * 2007-07-26 2009-12-10 Fujifilm Corp 核酸増幅方法
EP2207896B1 (en) * 2007-10-04 2014-01-29 Commonwealth Scientific and Industrial Research Organisation Nucleic acid amplification
JP5618413B2 (ja) * 2007-10-08 2014-11-05 シンセティック ゲノミクス、インク. 大型核酸のアッセンブリー
AU2009214435C1 (en) * 2008-02-15 2014-07-17 Synthetic Genomics, Inc. Methods for in vitro joining and combinatorial assembly of nucleic acid molecules
EP3263718A1 (en) * 2011-09-16 2018-01-03 Lexogen GmbH Nucleic acid transcription method
EP4219706A1 (en) * 2012-08-16 2023-08-02 Synthetic Genomics, Inc. Automated dsdna synthesis using a digital to biological converter
US20140308710A1 (en) * 2012-12-13 2014-10-16 Synthetic Genomics, Inc. Peg-mediated assembly of nucleic acid molecules
EP3666904A1 (en) * 2018-12-14 2020-06-17 Lexogen GmbH Nucleic acid amplification and identification method

Also Published As

Publication number Publication date
US20190177759A1 (en) 2019-06-13
EP2931913B1 (en) 2018-10-17
CN109913519B (zh) 2022-12-02
JP2015536689A (ja) 2015-12-24
IL238918B (en) 2020-04-30
DK2931913T3 (en) 2018-11-26
EP2931913A4 (en) 2016-08-17
WO2014093535A1 (en) 2014-06-19
CN109913519A (zh) 2019-06-21
CA2893817C (en) 2021-03-09
WO2014093535A8 (en) 2015-07-02
JP7009337B2 (ja) 2022-01-25
SG10201704426UA (en) 2017-06-29
US20140308710A1 (en) 2014-10-16
CA2893817A1 (en) 2014-06-19
IL238918A0 (en) 2015-07-30
AU2013359293A1 (en) 2015-06-18
JP2021048883A (ja) 2021-04-01
CN104822840A (zh) 2015-08-05
EP2931913A1 (en) 2015-10-21
JP2019022503A (ja) 2019-02-14
AU2013359293B2 (en) 2017-11-02

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