SE8903726L - PROCEDURES FOR FIXING PROTEINS TO FIXED CARRIERS - Google Patents

PROCEDURES FOR FIXING PROTEINS TO FIXED CARRIERS

Info

Publication number
SE8903726L
SE8903726L SE8903726A SE8903726A SE8903726L SE 8903726 L SE8903726 L SE 8903726L SE 8903726 A SE8903726 A SE 8903726A SE 8903726 A SE8903726 A SE 8903726A SE 8903726 L SE8903726 L SE 8903726L
Authority
SE
Sweden
Prior art keywords
reaction step
functional groups
procedures
reagent
protein
Prior art date
Application number
SE8903726A
Other languages
Unknown language ( )
Swedish (sv)
Other versions
SE8903726D0 (en
Inventor
E Hadas
Original Assignee
Roehm Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roehm Gmbh filed Critical Roehm Gmbh
Publication of SE8903726D0 publication Critical patent/SE8903726D0/en
Publication of SE8903726L publication Critical patent/SE8903726L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/087—Acrylic polymers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
    • A61K47/58—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. poly[meth]acrylate, polyacrylamide, polystyrene, polyvinylpyrrolidone, polyvinylalcohol or polystyrene sulfonic acid resin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

A process for covalently binding biologically active proteins to solid carrier materials having functional groups on the surface, whereby, in a first reaction step, the particularly reactive amino groups of the protein are modified with a reagent which can be removed reversibly under controlled conditions, in a second reaction step the protein is reacted with the functional groups of the carrier material, in a third reaction step the remaining functional groups of the carrier material are chemically blocked and in the final reaction step the modifying groups formed from the reagent are split off in a controlled reaction is described.
SE8903726A 1988-11-18 1989-11-07 PROCEDURES FOR FIXING PROTEINS TO FIXED CARRIERS SE8903726L (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE3839020A DE3839020A1 (en) 1988-11-18 1988-11-18 FIXING OF PROTEINS ON SOLID CARRIERS

Publications (2)

Publication Number Publication Date
SE8903726D0 SE8903726D0 (en) 1989-11-07
SE8903726L true SE8903726L (en) 1990-05-19

Family

ID=6367420

Family Applications (1)

Application Number Title Priority Date Filing Date
SE8903726A SE8903726L (en) 1988-11-18 1989-11-07 PROCEDURES FOR FIXING PROTEINS TO FIXED CARRIERS

Country Status (6)

Country Link
CH (1) CH679399A5 (en)
DE (1) DE3839020A1 (en)
FR (1) FR2639357B1 (en)
GB (1) GB2225017B (en)
IT (1) IT1233901B (en)
SE (1) SE8903726L (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9014689D0 (en) * 1990-07-02 1990-08-22 Tioxide Group Services Ltd Supports for active entities

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3650901A (en) * 1968-09-27 1972-03-21 Yeda Res & Dev Polymeric enzyme products
US3853708A (en) * 1970-01-23 1974-12-10 Exploaterings Ab Tbf Coupling biologically active substances to oxirane-containing polymers
SU443902A1 (en) * 1973-09-13 1974-09-25 Таллинский Политехнический Институт The method of obtaining enzymes immobilized in the structure of polymerization gels
DE2722751C2 (en) * 1977-05-20 1990-06-21 Röhm GmbH, 6100 Darmstadt Bead polymer with a particle diameter of 5 to 1000 μm and an internal cavity
US4652524A (en) * 1980-10-02 1987-03-24 Ivan E. Modrovich Soluble stabilized enzymes
DE3135541A1 (en) * 1981-09-08 1983-03-24 Bayer Ag, 5090 Leverkusen Derivatives of kallikrein-trypsin inhibitor (BPTI), carrier-bound BPTI derivatives, their preparation and their use for preparing the enzymes trypsin, chymotrypsin and kallikrein in pure form
CA1203164A (en) * 1982-03-09 1986-04-15 Thomas J. Mckearn Antibody conjugates
US4734362A (en) * 1986-02-03 1988-03-29 Cambridge Bioscience Corporation Process for purifying recombinant proteins, and products thereof

Also Published As

Publication number Publication date
DE3839020A1 (en) 1990-05-23
GB2225017A (en) 1990-05-23
IT1233901B (en) 1992-04-21
FR2639357B1 (en) 1994-07-29
GB8925240D0 (en) 1989-12-28
FR2639357A1 (en) 1990-05-25
IT8967810A0 (en) 1989-09-28
SE8903726D0 (en) 1989-11-07
GB2225017B (en) 1992-09-09
CH679399A5 (en) 1992-02-14

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