SE8903726D0 - FIXING OF PROTEINS AND FIXED CARRIERS - Google Patents
FIXING OF PROTEINS AND FIXED CARRIERSInfo
- Publication number
- SE8903726D0 SE8903726D0 SE8903726A SE8903726A SE8903726D0 SE 8903726 D0 SE8903726 D0 SE 8903726D0 SE 8903726 A SE8903726 A SE 8903726A SE 8903726 A SE8903726 A SE 8903726A SE 8903726 D0 SE8903726 D0 SE 8903726D0
- Authority
- SE
- Sweden
- Prior art keywords
- reaction step
- functional groups
- proteins
- fixing
- reagent
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
- C12N11/087—Acrylic polymers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
- A61K47/58—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. poly[meth]acrylate, polyacrylamide, polystyrene, polyvinylpyrrolidone, polyvinylalcohol or polystyrene sulfonic acid resin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Cell Biology (AREA)
- Public Health (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Food Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
A process for covalently binding biologically active proteins to solid carrier materials having functional groups on the surface, whereby, in a first reaction step, the particularly reactive amino groups of the protein are modified with a reagent which can be removed reversibly under controlled conditions, in a second reaction step the protein is reacted with the functional groups of the carrier material, in a third reaction step the remaining functional groups of the carrier material are chemically blocked and in the final reaction step the modifying groups formed from the reagent are split off in a controlled reaction is described.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE3839020A DE3839020A1 (en) | 1988-11-18 | 1988-11-18 | FIXING OF PROTEINS ON SOLID CARRIERS |
Publications (2)
Publication Number | Publication Date |
---|---|
SE8903726D0 true SE8903726D0 (en) | 1989-11-07 |
SE8903726L SE8903726L (en) | 1990-05-19 |
Family
ID=6367420
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SE8903726A SE8903726L (en) | 1988-11-18 | 1989-11-07 | PROCEDURES FOR FIXING PROTEINS TO FIXED CARRIERS |
Country Status (6)
Country | Link |
---|---|
CH (1) | CH679399A5 (en) |
DE (1) | DE3839020A1 (en) |
FR (1) | FR2639357B1 (en) |
GB (1) | GB2225017B (en) |
IT (1) | IT1233901B (en) |
SE (1) | SE8903726L (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9014689D0 (en) * | 1990-07-02 | 1990-08-22 | Tioxide Group Services Ltd | Supports for active entities |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3650901A (en) * | 1968-09-27 | 1972-03-21 | Yeda Res & Dev | Polymeric enzyme products |
US3853708A (en) * | 1970-01-23 | 1974-12-10 | Exploaterings Ab Tbf | Coupling biologically active substances to oxirane-containing polymers |
SU443902A1 (en) * | 1973-09-13 | 1974-09-25 | Таллинский Политехнический Институт | The method of obtaining enzymes immobilized in the structure of polymerization gels |
DE2722751C2 (en) * | 1977-05-20 | 1990-06-21 | Röhm GmbH, 6100 Darmstadt | Bead polymer with a particle diameter of 5 to 1000 μm and an internal cavity |
US4652524A (en) * | 1980-10-02 | 1987-03-24 | Ivan E. Modrovich | Soluble stabilized enzymes |
DE3135541A1 (en) * | 1981-09-08 | 1983-03-24 | Bayer Ag, 5090 Leverkusen | Derivatives of kallikrein-trypsin inhibitor (BPTI), carrier-bound BPTI derivatives, their preparation and their use for preparing the enzymes trypsin, chymotrypsin and kallikrein in pure form |
CA1203164A (en) * | 1982-03-09 | 1986-04-15 | Thomas J. Mckearn | Antibody conjugates |
US4734362A (en) * | 1986-02-03 | 1988-03-29 | Cambridge Bioscience Corporation | Process for purifying recombinant proteins, and products thereof |
-
1988
- 1988-11-18 DE DE3839020A patent/DE3839020A1/en not_active Withdrawn
-
1989
- 1989-09-28 IT IT8967810A patent/IT1233901B/en active
- 1989-10-12 FR FR898913325A patent/FR2639357B1/en not_active Expired - Fee Related
- 1989-11-07 SE SE8903726A patent/SE8903726L/en not_active Application Discontinuation
- 1989-11-08 GB GB8925240A patent/GB2225017B/en not_active Expired - Lifetime
- 1989-11-09 CH CH4035/89A patent/CH679399A5/de not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
DE3839020A1 (en) | 1990-05-23 |
IT8967810A0 (en) | 1989-09-28 |
GB2225017B (en) | 1992-09-09 |
GB2225017A (en) | 1990-05-23 |
CH679399A5 (en) | 1992-02-14 |
FR2639357A1 (en) | 1990-05-25 |
GB8925240D0 (en) | 1989-12-28 |
IT1233901B (en) | 1992-04-21 |
FR2639357B1 (en) | 1994-07-29 |
SE8903726L (en) | 1990-05-19 |
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Legal Events
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---|---|---|---|
NAV | Patent application has lapsed |
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