SE8803566D0 - RECOMBINANT, MAENNISCOINTERLEUKIN-2 SYNTHESIS CODING PLASMID DNA OF PPR-IL2-19 TYPE, PROCEDURAL FOOD CONSTRUCTION OF THIS PLASMID DNA AND MAENNISCOINTERLEUKIN-2 PRODUCT, NAME -IL2-19) - Google Patents

RECOMBINANT, MAENNISCOINTERLEUKIN-2 SYNTHESIS CODING PLASMID DNA OF PPR-IL2-19 TYPE, PROCEDURAL FOOD CONSTRUCTION OF THIS PLASMID DNA AND MAENNISCOINTERLEUKIN-2 PRODUCT, NAME -IL2-19)

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Publication number
SE8803566D0
SE8803566D0 SE8803566A SE8803566A SE8803566D0 SE 8803566 D0 SE8803566 D0 SE 8803566D0 SE 8803566 A SE8803566 A SE 8803566A SE 8803566 A SE8803566 A SE 8803566A SE 8803566 D0 SE8803566 D0 SE 8803566D0
Authority
SE
Sweden
Prior art keywords
plasmid
ppr
plasmid dna
maenniscointerleukin
paa1213
Prior art date
Application number
SE8803566A
Other languages
Swedish (sv)
Other versions
SE8803566L (en
Inventor
V G Debabov
E Y Gren
J I Kozlov
S V Mashko
A Y Strongin
V E Sterkin
V L Jurin
S V Kostrov
A J Tsimanis
A Y Avot
N V Romanchikova
A I Kosikov
M E Trukhan
A V Mochulsky
T V Chernekova
E A Nosovskaya
M A Kolevatykh
S M Mazel
Original Assignee
Inst Organicheskogo Sinteza Ak
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Organicheskogo Sinteza Ak filed Critical Inst Organicheskogo Sinteza Ak
Publication of SE8803566L publication Critical patent/SE8803566L/en
Publication of SE8803566D0 publication Critical patent/SE8803566D0/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/55—IL-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/73—Expression systems using phage (lambda) regulatory sequences

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

A recombinant plasmid DNA pPR-IL2-19, coding for synthesis of human interleukin-2 has a size of 3.85 k.b.p. and consists of the following elements: BamHI-BglII, a fragment of the size of 3.4 k.b.p. of vector plasmid pPR124B obtained on the basis of pPR40 and pML24; Cfr13-BglII, a fragment of the size of 0.45 k.b.p. of plasmid pAA1213-23B obtained by modifying plasmid pAA1213-23 constructed on the basis of plasmid pBR-322 containing the kDNA-copy of the gene of human interleukin-2. A method of construction of the above recombinant plasmid DNA consists in obtaining, by modifying plasmid pAA1213-23, plasmid pAA1213-23B, a fragment of which, together with the gene of interleukin-2, is integrated into vector plasmid pPR-IL2-19, and in transforming, by the thus obtained mixture, cells of E. coli C600 and in separating plasmid pPR-IL2-19 from the clones grown on a selective medium. A strain of bacteria E. coli VNIIGENETIKA VL 903 (pPR-IL2-19) as producer of human interleukin-2 deposited on 12.01.87 under No. 1869 at the culture collection of the USSR Research Institute for Antibiotics.
SE8803566A 1987-02-09 1988-10-07 RECOMBINANT, MAENNISCOINTERLEUKIN-2 SYNTHESIS CODING PLASMID DNA OF PPR-IL2-19 TYPE, PROCEDURAL FOOD CONSTRUCTION OF THIS PLASMID DNA AND MAENNISCOINTERLEUKIN-2 PRODUCT, NAME -IL2-19) SE8803566D0 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SU874191353A SU1703693A1 (en) 1987-02-09 1987-02-09 Dna ppr-il 2-19 recombination plasmid which codes human interleucine-2 synthesis and structure escherichia coli strain, human interleucine-2 producent

Publications (2)

Publication Number Publication Date
SE8803566L SE8803566L (en) 1988-10-07
SE8803566D0 true SE8803566D0 (en) 1988-10-07

Family

ID=21284358

Family Applications (1)

Application Number Title Priority Date Filing Date
SE8803566A SE8803566D0 (en) 1987-02-09 1988-10-07 RECOMBINANT, MAENNISCOINTERLEUKIN-2 SYNTHESIS CODING PLASMID DNA OF PPR-IL2-19 TYPE, PROCEDURAL FOOD CONSTRUCTION OF THIS PLASMID DNA AND MAENNISCOINTERLEUKIN-2 PRODUCT, NAME -IL2-19)

Country Status (10)

Country Link
JP (1) JPH01502639A (en)
CH (1) CH676997A5 (en)
DE (1) DE3890052T1 (en)
FI (1) FI884557A (en)
GB (1) GB2210373A (en)
HU (1) HUT50873A (en)
NL (1) NL8820102A (en)
SE (1) SE8803566D0 (en)
SU (1) SU1703693A1 (en)
WO (1) WO1988005818A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6068993A (en) * 1997-07-02 2000-05-30 Biobras Sa Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58116498A (en) * 1981-12-28 1983-07-11 Takeda Chem Ind Ltd Novel messenger rna coding il-2, its preparation and preparation of il-2 using it
JPH0646957B2 (en) * 1985-03-11 1994-06-22 武田薬品工業株式会社 Method for producing interleukin-2
WO1986006410A1 (en) * 1985-04-30 1986-11-06 Takeda Chemical Industries, Ltd. Process for increasing yield of interleukin-2

Also Published As

Publication number Publication date
FI884557A0 (en) 1988-10-04
SU1703693A1 (en) 1992-01-07
FI884557A (en) 1988-10-04
WO1988005818A1 (en) 1988-08-11
NL8820102A (en) 1989-01-02
SE8803566L (en) 1988-10-07
GB8823729D0 (en) 1988-12-07
CH676997A5 (en) 1991-03-28
DE3890052T1 (en) 1989-04-13
GB2210373A (en) 1989-06-07
JPH01502639A (en) 1989-09-14
HUT50873A (en) 1990-03-28

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