SE8500201L - PROCEDURE FOR ANALYSIS OF DNA FRAGMENT - Google Patents

PROCEDURE FOR ANALYSIS OF DNA FRAGMENT

Info

Publication number
SE8500201L
SE8500201L SE8500201A SE8500201A SE8500201L SE 8500201 L SE8500201 L SE 8500201L SE 8500201 A SE8500201 A SE 8500201A SE 8500201 A SE8500201 A SE 8500201A SE 8500201 L SE8500201 L SE 8500201L
Authority
SE
Sweden
Prior art keywords
fragments
gel
analysis
procedure
dna fragments
Prior art date
Application number
SE8500201A
Other languages
Swedish (sv)
Other versions
SE8500201D0 (en
SE456348B (en
Inventor
Leroy E Hood
Michael W Hunkapiller
Lloyd M Smith
Tim J Hunkapiller
Original Assignee
California Inst Of Techn
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by California Inst Of Techn filed Critical California Inst Of Techn
Publication of SE8500201D0 publication Critical patent/SE8500201D0/en
Publication of SE8500201L publication Critical patent/SE8500201L/en
Publication of SE456348B publication Critical patent/SE456348B/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Wood Science & Technology (AREA)
  • Pathology (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A method of sequencing DNA comprises sequencing the DNA by producing DNA fragments therefrom labelled with a colored or fluorescent tag, separating the fragments by gel electrophoresis and determining the relative positions of the fragments on the gel. This method may be effected utilising a system comprising: a source of tagged DNA fragments; a zone for containing an electrophoresis gel; means for introducing the tagged DNA fragments to said zone; and photometric means for monitoring said tagged DNA fragments as they move through said gel.
SE8500201A 1984-01-16 1985-01-16 PROCEDURE FOR ELECTROPHORETIC ANALYSIS OF DNA FRAGMENTS SE456348B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US57097384A 1984-01-16 1984-01-16
US68901385A 1985-01-02 1985-01-02

Publications (3)

Publication Number Publication Date
SE8500201D0 SE8500201D0 (en) 1985-01-16
SE8500201L true SE8500201L (en) 1985-07-17
SE456348B SE456348B (en) 1988-09-26

Family

ID=27075453

Family Applications (1)

Application Number Title Priority Date Filing Date
SE8500201A SE456348B (en) 1984-01-16 1985-01-16 PROCEDURE FOR ELECTROPHORETIC ANALYSIS OF DNA FRAGMENTS

Country Status (5)

Country Link
CA (1) CA1258611A (en)
DE (1) DE3501306C2 (en)
FR (1) FR2558262B1 (en)
GB (1) GB2155176B (en)
SE (1) SE456348B (en)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4865968A (en) * 1985-04-01 1989-09-12 The Salk Institute For Biological Studies DNA sequencing
US4855225A (en) * 1986-02-07 1989-08-08 Applied Biosystems, Inc. Method of detecting electrophoretically separated oligonucleotides
US4811218A (en) * 1986-06-02 1989-03-07 Applied Biosystems, Inc. Real time scanning electrophoresis apparatus for DNA sequencing
DE3618605A1 (en) * 1986-06-03 1987-12-10 Europ Lab Molekularbiolog DEVICE FOR DETECTING SUBSTANCES INCENTIVELY TO PHOTON EMISSION
DE3621631A1 (en) * 1986-06-27 1988-01-14 Hirschmann Geraetebau Gmbh & C DEVICE FOR IMAGING AN OBJECT ON AN ELECTROOPTIC CONVERTER
US5242796A (en) * 1986-07-02 1993-09-07 E. I. Du Pont De Nemours And Company Method, system and reagents for DNA sequencing
CA1340806C (en) * 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing
JPH0799353B2 (en) * 1987-03-31 1995-10-25 株式会社島津製作所 Nucleotide sequencer
DE3752148T2 (en) * 1987-06-09 1998-09-17 Perkin Elmer Corp Real time scanner in an electrophoresis apparatus for DNA sequence determination
US4833332A (en) * 1987-06-12 1989-05-23 E. I. Du Pont De Nemours And Company Scanning fluorescent detection system
US5102785A (en) * 1987-09-28 1992-04-07 E. I. Du Pont De Nemours And Company Method of gene mapping
JPH01224657A (en) * 1988-03-04 1989-09-07 Hitachi Ltd Device for determining base sequence of nucleic acid
EP0341928A1 (en) * 1988-05-10 1989-11-15 AMERSHAM INTERNATIONAL plc Improvements relating to surface plasmon resonance sensors
SE8802573D0 (en) * 1988-07-08 1988-07-08 Wallac Oy MULTI-LABEL TIME-RESOLVED FLUORESCENCE ANALYSIS OF NUCLEIC ACID SEQUENCES USING LANTHANIDE CHELATES
FR2636738B1 (en) * 1988-09-20 1992-12-11 Commissariat Energie Atomique DNA SEQUENCING PROCESS AND INSTALLATION
FR2636739B1 (en) * 1988-09-20 1993-02-12 Commissariat Energie Atomique METHOD AND INSTALLATION FOR IDENTIFYING THE BASES OF DNA
US5061361A (en) * 1989-03-06 1991-10-29 Hewlett-Packard Company Capillary zone electrophoresis cell system
FR2649487B1 (en) * 1989-06-21 1991-09-20 Europhor Sa ELECTROPHORESIS CAPILLARY WITH INCORPORATED OPTICAL DEVICE
US5274240A (en) * 1990-01-12 1993-12-28 The Regents Of The University Of California Capillary array confocal fluorescence scanner and method
FR2671407B1 (en) * 1991-01-08 1994-08-05 Europhor Sa METHOD OF ANALYSIS BY CAPILLARY ELECTROPHORESIS WITH FLUORESCENCE DETECTION, AND DEVICES FOR IMPLEMENTING SAME.
US5203339A (en) * 1991-06-28 1993-04-20 The Government Of The United States Of America As Represented By The Secretary Of The Department Health And Human Services Method and apparatus for imaging a physical parameter in turbid media using diffuse waves
US5273638A (en) * 1991-09-30 1993-12-28 Beckman Instruments, Inc. Nucleotide sequence determination employing matched dideoxynucleotide terminator concentrations
DE69221423T2 (en) * 1991-09-30 1998-03-19 Beckman Instruments Inc Improved fluorescence detection of samples in a capillary tube
US5484571A (en) * 1991-10-08 1996-01-16 Beckman Instruments, Inc. Enhanced fluorescence detection of samples in capillary column
AU3816993A (en) * 1992-03-19 1993-10-21 Regents Of The University Of California, The Multiple tag labeling method for DNA sequencing
JPH05332992A (en) * 1992-05-29 1993-12-17 Shimadzu Corp Electrophoresis device
DE4336911C2 (en) * 1993-10-28 2002-11-21 Biotechnolog Forschung Gmbh Primer production process and template bank therefor
JP3068413B2 (en) * 1994-07-13 2000-07-24 日立電子エンジニアリング株式会社 DNA base sequencer
US5675155A (en) * 1995-04-26 1997-10-07 Beckman Instruments, Inc. Multicapillary fluorescent detection system
US6110683A (en) * 1999-01-08 2000-08-29 Commonwealth Biotechnologies, Inc. Automated DNA Sequencer loading dye which contains a lane tracking aid

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1205028A (en) * 1981-07-01 1986-05-27 Jerald C. Hinshaw Fluorescent chelates and labeled specific binding reagents prepared therefrom
CA1180647A (en) * 1981-07-17 1985-01-08 Cavit Akin Light-emitting polynucleotide hybridization diagnostic method
FR2519005B1 (en) * 1981-12-29 1985-10-25 Pasteur Institut DNA FRAGMENTS MARKED WITH AT LEAST ONE OF THEIR ENDS ENDANGERED BY MODIFIED RIBONUCLEOTIDES RECOGNIZABLE BY AFFINOUS MOLECULES AND METHOD FOR CARRYING OUT ANALYSIS OF SUCH DNA FRAGMENTS
DK105582A (en) * 1982-03-11 1983-09-12 Nordisk Insulinlab PROCEDURE FOR DETERMINING HUMAN HLA-D (R) TISSUE TYPES AND REVERSE FOR USING THE PROCEDURE
US4490472A (en) * 1982-06-17 1984-12-25 Imreg, Inc. Sensitive tests for malignancies based on DNA detection

Also Published As

Publication number Publication date
FR2558262A1 (en) 1985-07-19
FR2558262B1 (en) 1989-04-07
DE3501306C2 (en) 1998-09-24
GB2155176B (en) 1988-05-11
SE8500201D0 (en) 1985-01-16
GB2155176A (en) 1985-09-18
CA1258611A (en) 1989-08-22
SE456348B (en) 1988-09-26
DE3501306A1 (en) 1985-07-25
GB8500960D0 (en) 1985-02-20

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