SE7614532L - PROCEDURE FOR DETERMINATION OF ACID PHASHATASE - Google Patents

PROCEDURE FOR DETERMINATION OF ACID PHASHATASE

Info

Publication number
SE7614532L
SE7614532L SE7614532A SE7614532A SE7614532L SE 7614532 L SE7614532 L SE 7614532L SE 7614532 A SE7614532 A SE 7614532A SE 7614532 A SE7614532 A SE 7614532A SE 7614532 L SE7614532 L SE 7614532L
Authority
SE
Sweden
Prior art keywords
mmol
determination
substrate
acid
phashatase
Prior art date
Application number
SE7614532A
Other languages
Unknown language ( )
Swedish (sv)
Other versions
SE425098B (en
Inventor
H Gallati
M Roth
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of SE7614532L publication Critical patent/SE7614532L/en
Publication of SE425098B publication Critical patent/SE425098B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

A method and a relevant set of reagents for the determination of an acid phosphatase of human origin, especially prostate phosphatase, in a body fluid, are described. The method comprises subjecting a substrate solution to the enzymatic action of the body fluid to be investigated at an incubation temperature between 20 DEG C and 45 DEG C, and measuring the substrate conversion. Used in this are a phosphomonoester as substrate, a buffer substance to adjust to a pH between 4.5 and 6.5, and an activator containing at least one straight-chain alcohol with 4-6 C atoms. The substrate solution contains 50 mu mol/l to 50 mmol/l phosphomonoester, 50-300 mmol/l buffer substance and 10-300 mmol/l activator.
SE7614532A 1975-12-24 1976-12-23 PROCEDURE AND REAGENTS FOR DETERMINING ACID PHOSPHATUS SE425098B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CH1677975A CH625833A5 (en) 1975-12-24 1975-12-24 Method for the determination of acid phosphatase

Publications (2)

Publication Number Publication Date
SE7614532L true SE7614532L (en) 1977-06-25
SE425098B SE425098B (en) 1982-08-30

Family

ID=4420153

Family Applications (1)

Application Number Title Priority Date Filing Date
SE7614532A SE425098B (en) 1975-12-24 1976-12-23 PROCEDURE AND REAGENTS FOR DETERMINING ACID PHOSPHATUS

Country Status (11)

Country Link
JP (1) JPS586480B2 (en)
CA (1) CA1070597A (en)
CH (1) CH625833A5 (en)
DD (1) DD128970A5 (en)
DE (1) DE2658634C2 (en)
DK (1) DK582776A (en)
FR (1) FR2336683A1 (en)
GB (1) GB1563517A (en)
IT (1) IT1154005B (en)
NL (1) NL178798C (en)
SE (1) SE425098B (en)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3425912A (en) * 1966-11-14 1969-02-04 Smithkline Corp Laboratory reagent for assay of alkaline phosphatase
GB1263202A (en) * 1970-01-14 1972-02-09 Pfizer Alkaline phosphatase test
US3823071A (en) * 1970-12-21 1974-07-09 Worthington Biochem Corp Prostatic acid phosphatase determination
DE2115748C3 (en) * 1971-03-31 1981-07-16 Boehringer Mannheim Gmbh, 6800 Mannheim Method for the determination of acid phosphatase

Also Published As

Publication number Publication date
NL7613768A (en) 1977-06-28
NL178798B (en) 1985-12-16
DE2658634A1 (en) 1977-07-07
IT1154005B (en) 1987-01-21
DE2658634C2 (en) 1984-03-01
CH625833A5 (en) 1981-10-15
NL178798C (en) 1986-05-16
DK582776A (en) 1977-06-25
JPS586480B2 (en) 1983-02-04
CA1070597A (en) 1980-01-29
SE425098B (en) 1982-08-30
GB1563517A (en) 1980-03-26
DD128970A5 (en) 1977-12-21
FR2336683A1 (en) 1977-07-22
JPS5282386A (en) 1977-07-09
FR2336683B1 (en) 1980-10-03

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