SE543088C2 - Vessel and processes for culturing and harvesting benthic diatoms - Google Patents

Vessel and processes for culturing and harvesting benthic diatoms

Info

Publication number
SE543088C2
SE543088C2 SE1950701A SE1950701A SE543088C2 SE 543088 C2 SE543088 C2 SE 543088C2 SE 1950701 A SE1950701 A SE 1950701A SE 1950701 A SE1950701 A SE 1950701A SE 543088 C2 SE543088 C2 SE 543088C2
Authority
SE
Sweden
Prior art keywords
harvesting
tank
compartment
vessel
water
Prior art date
Application number
SE1950701A
Other languages
Swedish (sv)
Other versions
SE1950701A1 (en
Inventor
Gustav Knutsson
Original Assignee
Swedish Algae Factory Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Swedish Algae Factory Ab filed Critical Swedish Algae Factory Ab
Priority to SE1950701A priority Critical patent/SE543088C2/en
Priority to PCT/SE2020/050588 priority patent/WO2020251461A1/en
Publication of SE1950701A1 publication Critical patent/SE1950701A1/en
Publication of SE543088C2 publication Critical patent/SE543088C2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G33/00—Cultivation of seaweed or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/04—Flat or tray type, drawers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/22—Settling tanks; Sedimentation by gravity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/22—Processes using, or culture media containing, cellulose or hydrolysates thereof

Abstract

A vessel for culturing and harvesting benthic diatoms comprising a culturing tank comprising a substantially flat base on which a biofilm of benthic diatoms is formable, and a wall defining a receptacle for water and the biofilm, a harvesting compartment adjacent to and forming a trough for the culturing tank, the harvesting compartment being provided with at least one aperture at its lower portion for the collection of the biofilm; and, an overflow compartment adjacent to the harvesting compartment and distal the culturing tank, a proximal wall of the overflow compartment having an uppermost edge defining the maximum height of water receivable in the vessel.

Description

VESSEL AND PROCESSES FOR CULTURING AND HARVESTINGBENTHIC DIATOMS Field of the InventionThe present disclosure relates to a vessel for the culturing and harvesting of abiof1lm of benthic diatoms. Specifically it relates to a vessel comprising a harvesting compartment and an overflow compartment.
Background of the invention Diatoms are a group of microalgae found in oceans and other Waterways. Theyare unicellular organisms Which have a shell, a frustule, comprising silica. Culturing andharvesting of diatoms has traditionally been perforrned in open Waterways or on alaboratory scale using regular lab equipment.
Benthic diatoms are bottom dwelling diatoms Which grow attached to benthicsubstrates.
Lebeau et al, Díatom cultívatíon and bíotechnologícally relevant products. PartI: Cultívatíon at various scales, Appl Microbiol Biotechnol (2002) describes varioussystems for culturing diatoms. However, vessels and processes for the efficient culturingand specifically harvesting of diatoms have not been developed. As disclosed in thereferenced article most non lab-scale systems are simple tanks or vessels Without anymeans to eff1ciently harvest the diatoms.
Improved vessels for the culturing and harvesting of diatoms Would be advantageous.
Summary of the invention Accordingly, the present invention preferably seeks to mitigate, alleviate oreliminate one or more of the above-identified deficiencies in the art and disadvantagessingly or in any combination and solves at least the above mentioned problems byproviding a vessel for culturing and harvesting benthic diatoms comprising a culturingtank comprising a substantially flat base on Which a biof1lm of benthic diatoms isforrnable, and a Wall def1ning a receptacle for Water and the biofilm, a harvesting compartment adjacent to and forrning a trough for the culturing tank, the harvesting compartment being provided with at least one aperture at its lower portion for the collection of the biofilm; and, an overflow compartment adj acent to the harvesting compartment and distal the culturing tank, a proximal wall of the overflow compartment having an upperrnost edge def1ning the maximum height of water receivable in the vessel.A method for culturing a biofilm of benthic diatoms is also provided.
Furthermore, a method for harvesting a biofilm of benthic diatoms is provided.
Further advantageous embodiments are disclosed in the appended and dependent patent claims.
Brief description of the drawings These and other aspects, features and advantages of which the invention iscapable will be apparent and elucidated from the following description of embodimentsof the present invention, reference being made to the accompanying drawings, in which Fig. 1 is a perspective view of a vessel according to an aspect. A portion of theculturing tank is shown with dotted lines.
Fig. 2 is a perspective side-view of a vessel according to an aspect. A portion ofthe culturing tank is shown with dotted lines.
Fig. 3 is a partial perspective side-view and a partial schematic of a systemcomprising a vessel according to an aspect, a harvesting tank, a system tank and a recirculation system.
Detailed description Figures 1 to 3 show a vessel 1 for culturing and harvesting benthic diatomscomprising a culturing tank 100, a harvesting compartment 200 and an overflowcompartment 300. The culturing tank 100 comprises a base 101 on which a biofilm ofbenthic diatoms is forrnable. The harvesting compartment 200 is provided adj acent to the culturing tank 100. The overflow compartment 300 is provided adjacent the harvesting compartment 200 and comprises a Wall 301 Which def1nes the height at Which Water maybe receivable in the culturing tank 100.
The vessel 1 for culturing and harvesting enables efficient culturing andharvesting of a biof1lm of benthic diatoms. The diatom biof1lm may be harvested fromthe harvesting compartment 200. Water, substantially free of the biof1lm may be receivedin the overflow compartment 300.
The culturing tank 100 comprises a base 101. The base 101 may be substantiallyplanar and forms a surface suitable for culturing a biof1lm of benthic diatoms. Theculturing tank 100 comprises a Wall 102 connected to the perimeter of the base 101 Whichdefines a receptacle for Water and the biofilm. The base 101 of the culturing tank 100 maybe substantially rectangular. The Wall 102 may therefore comprise a plurality ofindividual Wall members 102, each forrning a portion of the Wall 102. The Wall extendsat least partially perpendicular to the base 101 such that the receptacle is formed.
The culturing tank 100 may be considered to have a first end 103 and a secondend 104. The second end 104 is proximal the harvesting compartment 200. Each of theends 103, 104 may be perpendicular to the longitudinal axis of extension of the base 101of the culturing tank 100.
The base 101 is generally free of interrnediate structures such as dividers or thelike such that the volume of Water and biof1lm present in the tank is continuous and notdivided in to sections or channels. Ideally the base 101 is substantially flat. A flat/planarbase in combination With the harvesting compartment 200 results in easier and quickerharvesting of the biof1lm.
The harvesting compartment 200 is adjacent to the culturing tank 100. Theharvesting compartment 200 may abut the culturing tank 100. The harvestingcompartment 200 forms a trough in connection to the culturing tank 100. The harvestingcompartment 200 has an opening 203 via Which Water and biof1lm may flow in to theharvesting compartment 200. The harvesting compartment 200 is formed by a firstproximal Wall 204 adjacent the culturing tank 100, a base 205, a distal Wall 202, and apair of side-Walls 208. The proximal Wall 204, the base 205, and the distal Wall 202 forma receptacle for Water and/or biof1lm. The harvesting compartment 200 may have a substantially rectangular base 205 having its longitudinal extension perpendicular to the longitudinal extension of the base 101 of the culturing tank 100. The harvestingcompartment 200 may be aligned and parallel to the edge at the second end 104 of theculturing tank 100. The harvesting compartment 200 has a width corresponding to thewidth of the culturing tank 100. The harvesting compartment 200 may be wider than thewidth of the culturing tank 100. The base 205 of the harvesting compartment 200 isarranged at a height below the base 101 of the culturing tank 100. The proximal wall 204extends perpendicular from the base 205 of the harvesting compartment 200 upwards.The top edge 206 of the proximal wall 204 may be at a height greater than the height ofthe base 101 of the culturing tank 100, or it may be at the same height as the height of thebase 101 of the culturing tank. Ideally, the top edge 206 is at the same height as the base101 of the culturing tank 100 such that the top edge 206 and base 101 form a flat opening203 for the ingress of water and/or biof1lm. The length of the opening 203 generallycorresponds to the width of the culturing tank 100. That is, the harvesting tank 200 isopen along its entire length in connection with the base 101 of the culturing tank 200.
The lower portion the harvesting compartment 200 is provided with an aperture201 for receiving water and/or benthic diatoms. The aperture may be provided in the base205 of the harvesting compartment 200 to ensure that the greatest proportion of waterand/or diatoms are collected at the aperture 201. Water and/or diatoms may be removedfrom the harvesting compartment via the aperture 201. The aperture 201 is provided incommunication with a harvesting valve 207 which is openable to allow water and/ordiatoms to flow from the harvesting compartment 200. To ease accessibility andmaintenance the aperture 201 may be provided near one of the pair of side-walls 208. Theaperture 201 may connect to the harvesting valve 207 via a pipe 209. The aperture 201and harvesting valve 207 are in fluidic communication with a harvesting tank (notshown). The harvesting tank is for receiving and storing diatoms cultured in the culturingtank 100 and harvested from the harvesting compartment 200.
The aperture 201 and pipe 209 are in communication with a drain valve 304. Theharvesting valve 207 and the drain valve 304 are controllable such that the flow of waterfrom the harvesting compartment 200 may be controlled.
The side-walls 208 extend upwards from the base 205 such that they are higherthan the wall 301 of the overflow compartment 300.
The overflow compartment 300 is arranged adjacent to the harvestingcompartment 200. The overflow compartment 300 may form a trough in connection tothe harvesting compartment and the culturing tank 100. The combined structure of theharvesting compartment 200 and the overflow compartment 300 may be considered asingle trough.
The overflow compartment 300 comprises a wall 301 proximal the harvestingcompartment 200, a base 305, a distal wall 306 and a pair of side-walls 308. The overflowcompartment 300 is distal the culturing tank 100, with respect to the harvestingcompartment 200. That is, the harvesting compartment 200 is arranged between theoverflow compartment 300 and the culturing tank 100. The overflow compartment 300receives water flowing over the wall 301. Water flows over the top edge 302 of the wall301 and in to the overflow compartment 300. Water flow from the harvestingcompartment 200 in to the overflow compartment 300. As with the harvestingcompartment 200, the overflow compartment 300 may have an elongated, substantiallyrectangular, base 305 having its longitudinal axis of extension aligned perpendicular tothe axis of extension of the culturing tank 200. The proximal wall 301, and the distal wall306 may extend at least partially perpendicular to the base 305 of the overflowcompartment 300 such that a receptacle for water is formed between the walls.
The wall 301 defines an opening, that is, the region above the top edge 302 ofthe wall, which defines the height and therein volume of water receivable in the vessel 1.The opening may be formed by other structures, such as a hole in the wall 302, or a holein the side walls 308, or the like, which define the height of water receivable in the vessel1.
The side-walls 308 extend upwards from the base 305 such that they are higherthan the proximal wall 301. The side-walls 308 and the distal wall 306 may havesubstantially the same height with respect to the base 305 of the overflow compartment300.
The proximal wall 301 of the overflow compartment may form the distal wall202 of the harvesting compartment 300. That is, the harvesting compartment 200 and theoverflow compartment 300 may share the wall. In such an arrangement, the harvesting compartment 200 and the overflow compartment 300 may be considered to be formed via a single receptacle with an internal dividing wall separating the harvesting compartment200 from the overflow compartment 300.
The lower portion of the overflow compartment 300 is provided with anrecirculation aperture 303 for receiving water. The recirculation aperture 303 may beprovided in the base 305 of the overflow compartment 300. Water removed from theoverflow compartment via the recirculation aperture 303. The recirculation aperture 303is provided in communication with a system tank 500 for storage of water, either duringharvesting or as an interrnediate storage step during culturing. To ease accessibility andmaintenance the recirculation aperture 303 may be provided near one of the pair of side-walls 306. The recirculation aperture 303 may connect to the drain valve 304 via a pipe309. The recirculation aperture 303 and valve 304 are in fluidic communication with asystem tank 500.
The drain valve 304 may be provided at a position between the recirculationaperture 303 and the harvest valve 207. The drain valve 304 is generally closed duringculturing of diatoms. During culturing water present in the overflow compartment 300may flow from the overflow compartment 300, via a portion of the pipe 309 to arecirculation system, comprising a recirculation pump and, optionally, the system tank500. Water from the overflow compartment 300 is subsequently retumed to the culturingtank 100 via the recirculation system.
The recirculation aperture 303 is generally open to the recirculation system viaa fluid conduit 310, during culturing. The fluid conduit 310 connects the overflowcompartment 300 to the recirculation system. The fluid conduit 310 is distal the drainvalve 304 and the harvest valve 207. The drain valve 304 is generally closed such thatwater exits the harvesting compartment 200 via flowing over the distal wall 202 and in tothe overflow compartment 300, subsequently water flows from the recirculation aperture303 via the fluid conduit 310 to the recirculation system. During culturing the harvestingvalve 207 and the drain valve 304 are closed such that water and/or diatoms do not flowvia the aperture 201 to the harvesting tank 207.
By forcing the water from the culturing tank 100, and the harvesting compartment 200 to flow over the distal wall 202 in to the overflow compartment 300 the flow of diatoms in a biof1lm, Which settle on the base 101 of the culturing tank 100, andharvesting compartment 200, is minimised.
The harvesting compartment 200 and the overflow compartment 300 may bearranged at an angle such that their respective bases 205, 305 are angled With respect tothe an absolute horizontal alignment. The absolute horizontal alignment is the angle ofthe top surface of Water present in the respective compartments 200, 300. For example,the bases 205, 305 of the compartments 200, 300 may be at greater than 0°, such as 1°, toan absolute horizontal alignment. This results in Water in the respective compartmentstending to gather at one of the side-Walls 208, 308 of both the harvesting compartment200 and the overflow compartment 300. Water can therefore more easily be extracted viathe apertures 201, 303 of the harvesting compartment 200, and/or the overflowcompartment 300. The culturing tank 100 may be aligned parallel to the alignment of theharvesting compartment 200 and overflow compartment 300 such that it is also at an angleto the horizontal.
The harvesting compartment 200, and the overflow compartment 300 may bemanufactured from a rigid material such as a rigid polymer, a glass or similar. Theharvesting compartment 200 and the overflow compartment 300 may be manufacturedfrom the same material or from different materials for their different components. Ideally,the harvesting compartment 200 and the overflow compartments 300 are manufacturedfrom a single piece of material as described above. For example, the harvestingcompartment 200 and the overflow compartment 300 may be manufactured from anopaque plastic. An opaque plastic is ideal as it reduces the potential for biof1lm growthWithin the harvesting compartment 200.
A system for culturing and harvesting a biof1lm of benthic diatoms is shown inFig. 3. The system comprises the vessel 1, a harvest tank 400, a system tank 500 and arecirculation system from the recirculation aperture 303 to the first end 103 of theculturing tank 100. The system tank 500 may be provided in connection to at least one,such as a plurality of vessels 1, and therefore, may have a volumetric capacity sufficientto store Water from a plurality of vessels 1. The harvest tank 400 may be provided inconnection to at least one, such as a plurality of vessels 1. The harvest tank 400 may form a receptacle for diatoms and Water harvested from a plurality of vessels 1.
A process for culturing and a process for harVesting a biof1lm of benthicdiatoms, using the culturing and harVesting Vessel 1, will now be disclosed.
During culturing water is provided to the first end 103 of the culturing tank 100.Water flows via the culturing tank 100 to the second end 104. The water provides nutritionto the biof1lm of diatoms and enables the population of benthic diatoms to increase,forrning a thicker biofilm on the base 101 of the culturing tank 100. Water flows from theculturing tank 100 to the harVesting compartment 200 Via the top edge 206 of the proximalwall 204. An incidental biof1lm may forrn on the base 205 of the harVesting compartment200. However, generally there is insufficient light to sustain a substantial population ofdiatoms in the harVesting compartment 200. During culturing the flow of water from theharVesting compartment 200 Via the aperture 201 is restricted. The harVest Valve 207 andthe drain Valve 304 are closed. Water flows from the harvesting compartment 200 to theoverflow compartment 300 via the top edge 302 of the proximal wall 301 of the overflowcompartment 300. Water collects in the overflow compartment 300 and thereafter flowsfrom the overflow compartment 300 to the recirculation system Via the fluidic conduit310. Water may be pumped from the overflow compartment Via the recirculation systemback to the first end 103 of the culturing tank 100. In this manner water may be circulatedVia the culturing tank 100, the harVesting compartment 200 and the overflowcompartment 300.
The process for harVesting generally comprises first stopping the recirculationsystem such that water no longer flows from the overflow compartment 300 to theculturing tank 100. Generally this inVolVes stopping the pumping of fluid from theoverflow compartment 300 to the culturing tank 100. Water may be redirected Via a valve(not shown) from the fluid conduit 310 to the system tank 500. Or if the system tank 500is part of the recirculation system, discontinuing the pumping of water from the systemto the culturing tank 100. The drain Valve 304 is subsequently opened. Water flowsthereafter from the harVesting compartment to the fluid conduit 310, Via the aperture 201and the open drain Valve 304, to the system tank 500. The system tank 500 has a Volumesufficient to store the total Volume of fluid in one or a plurality of culturing and harVestingVessels 1. Water draining from the harvesting compartment 200 does not comprise the majority of the biof1lm of benthic diatoms as they are at least partially adhered to the base 101 of the culturing tank 100. The Water drained to the system tank 500 is thereforesubstantially free of benthic diatoms.
After draining for a period of time a reduced volume of water and the biofilm ofbenthic diatoms is present on the base 101 of the culturing tank 100. The drain valve 304is thereafter closed such that water may no longer flow via the conduit 310 to the systerntank 500.
The harvest valve 207 is thereafter, or simultaneously with the closing of thedrain valve 304, opened. Water and/or diatoms may then flow from the harvestingcompartment 200 to the harvest tank 400.
Such that the biof1lm is harvested, the biof1lm may be mechanically displacedfrom the base 101 towards the harvesting compartment 200. The mechanicaldisplacement may be achieved by wiping, or otherwise displacing the biofilm along thebase 101 towards the harvesting compartment 200. The benthic diatoms, and a volume ofwater, will flow from the aperture 201 to the harvest tank 400 via the open harvest valve207. The harvested diatoms are thereafter present in the harvest tank 400. The harvestvalve 207 may thereafter be closed.
After harvesting, to re-initiate culturing, the recirculation system may be re-activated such that water is displaced from the system tank 500 back to the culturing tank100. Any benthic diatoms not harvested, or incidentally present in the system tank 500 Although, the present invention has been described above with reference tospecific embodiments, it is not intended to be limited to the specific form set forth herein.Rather, the invention is limited only by the accompanying claims.
In the claims, the term “comprises/comprising” does not exclude the presence ofother elements or steps. Additionally, although individual features may be included indifferent claims, these may possibly advantageously be combined, and the inclusion indifferent claims does not imply that a combination of features is not feasible and/oradvantageous. In addition, singular references do not exclude a plurality. The terms “a”,“an”, “f1rst”, “second” etc do not preclude a plurality. Reference signs in the claims areprovided merely as a clarifying example and shall not be construed as limiting the scope of the claims in any way.

Claims (10)

1. A vessel (1) for culturing and harvesting benthic diatoms comprising a culturing tank (100) comprising a substantially flat base (101) on Which abiof1lm of benthic diatoms is forrnable, and a Wall (102) def1ning a receptacle for Water and the biof1lm, a harvesting compartment (200) adjacent to and forrning a trough for theculturing tank (100), the harvesting compartment (200) being provided With atleast one aperture (201) at its lower portion for the collection of the biofilm; and, an overflow compartment (300) adjacent to the harvesting compartment (200)and distal the culturing tank (100), a Wall (301) of the overflow compartment(300), proximal the harvesting compartment (200) having an upperrnost edge (302) def1ning the maximum height of Water receivable in the vessel (1). The vessel (1) according to claim 1, Wherein a base (305) of the overflowcompartment (300) is arranged relatively lower than the base (101) of theculturing tank (100). The vessel (1) according to claim 1 or 2, Wherein the proximal Wall (301) of theoverflow compartment (300) forms a distal Wall (202) of the harvestingcompartment (200). The vessel (1) according to any of claim 1 to 3, Wherein the overflowcompartment is provided With at least one recirculation aperture (303) for receiving Water. The vessel (1) according to any of claims 1 to 4, Wherein the base (205) of theharvesting compartment (200), is provided at an angle to the horizontal such that Water and/or biof1lm collects in the vicinity of the aperture (201). 10. 11. The vessel (1) according to any of claims 1 to 5, Wherein the base (101) of theculturing tank (100) is substantially planar and free from structures such as dividers. The vessel (1) according to any of claims 1 to 6, Wherein the aperture (201) is fluidically connected to a harvesting tank for receiving and storing diatoms. The vessel (1) according to any of claims 1 to 7, Wherein the aperture (201) isprovided in connection to a harvesting tank for the collection of the diatoms and Water. The vessel (1) according to any of claims 1 to 8, Wherein the culturing tank(100) receives Water at a first end (103) and the harvesting compartment (200) and overfloW compartment (300) are provided at a second end (104). A method of culturing a biofilm of benthic diatoms in the vessel (1) accordingto any of claims 1 to 9, tlæ-e--grecirculation aperture (303) of the vessel (1) beingprovided in connection to a recirculation system comprising a pump, and asystem tank (5 00) for collecting Water from at least one vessel (1) in connectionto the pump, and the vessel (1) being provided With a volume of Water, the method comprising: -receiving Water from the culturing tank (100) in the overfloW compartment (300), and -pumping Water from the recirculation aperture (303) to a first end (103) of theculturing tank (1~Q3_É_1_QQ_) via the recirculation system. A method for harvesting a biofilm of benthic diatoms from the vessel (1) according to any of claims 1 to 41-02, comprising: -opening a drain valve (304) provided in connection the aperture (201) such thatWater floWs from the harvesting compartment (200) to a system tank (500) for the storage of Water; -draining a proportion of Water from the Vessel (l);-closing the drain Valve (304); -subsequently opening a harvest Valve (207) provided in connection to theaperture (201) such that diaton1s and Water flows from the harvesting conipartnient (200) to a harVest tank (400); and, -displacing the biof1ln1 on the base (l0l) of the culturing tank (l00) toWards theharvesting conipartnient (200) such that the biof1ln1 collects in the harvestingcon1partn1ent (200) and flows through the aperture (20l) to the harvesting tank.
SE1950701A 2019-06-13 2019-06-13 Vessel and processes for culturing and harvesting benthic diatoms SE543088C2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
SE1950701A SE543088C2 (en) 2019-06-13 2019-06-13 Vessel and processes for culturing and harvesting benthic diatoms
PCT/SE2020/050588 WO2020251461A1 (en) 2019-06-13 2020-06-09 Vessel and processes for culturing and harvesting benthic diatoms

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE1950701A SE543088C2 (en) 2019-06-13 2019-06-13 Vessel and processes for culturing and harvesting benthic diatoms

Publications (2)

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SE543088C2 true SE543088C2 (en) 2020-10-06

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WO (1) WO2020251461A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114847154A (en) * 2022-05-13 2022-08-05 河海大学 Device and method for monitoring and analyzing tidal flat mud snail movement among benthic microalgae

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR94705E (en) * 1966-06-01 1969-10-24 Inst Francais Du Petrole Improved method for cultivating algae and implementing device.
US3768200A (en) * 1971-07-16 1973-10-30 Research Corp Apparatus for the production of algae including a filtering medium
US8941222B2 (en) * 2010-11-11 2015-01-27 Advanced Semiconductor Engineering Inc. Wafer level semiconductor package and manufacturing methods thereof
AU2015325779B2 (en) * 2014-10-03 2020-02-06 Cambridge Filter Corporation Cultured algae water concentration system, method for operating cultured algae water concentration system, and method for concentrating algae water containing cultured algae
AU2017276704B2 (en) * 2016-06-08 2021-04-01 Swedish Algae Factory Ab Frustules extracted from benthic pennate diatoms harvested from an industrial biofilm process

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114847154A (en) * 2022-05-13 2022-08-05 河海大学 Device and method for monitoring and analyzing tidal flat mud snail movement among benthic microalgae
CN114847154B (en) * 2022-05-13 2022-12-27 河海大学 Device and method for monitoring and analyzing tidal flat mud snail movement among benthic microalgae

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WO2020251461A1 (en) 2020-12-17

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