SE425439B - Immunologisk analys med lantanidkelatkomplex som markor - Google Patents
Immunologisk analys med lantanidkelatkomplex som markorInfo
- Publication number
- SE425439B SE425439B SE8102753A SE8102753A SE425439B SE 425439 B SE425439 B SE 425439B SE 8102753 A SE8102753 A SE 8102753A SE 8102753 A SE8102753 A SE 8102753A SE 425439 B SE425439 B SE 425439B
- Authority
- SE
- Sweden
- Prior art keywords
- fluorescence
- lantanide
- marker
- substance
- detergent
- Prior art date
Links
- 230000001900 immune effect Effects 0.000 title claims description 3
- 238000004458 analytical method Methods 0.000 title description 2
- 238000000034 method Methods 0.000 claims abstract description 10
- 239000003550 marker Substances 0.000 claims abstract description 8
- 239000000126 substance Substances 0.000 claims abstract description 6
- 150000001875 compounds Chemical class 0.000 claims abstract description 5
- 239000003599 detergent Substances 0.000 claims abstract description 4
- 239000013543 active substance Substances 0.000 claims abstract 2
- 230000005855 radiation Effects 0.000 claims abstract 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 2
- 239000002879 Lewis base Substances 0.000 claims description 2
- 229920004890 Triton X-100 Polymers 0.000 claims description 2
- 239000002585 base Substances 0.000 claims description 2
- 150000007527 lewis bases Chemical class 0.000 claims description 2
- 239000012491 analyte Substances 0.000 claims 1
- 238000003776 cleavage reaction Methods 0.000 claims 1
- 238000007865 diluting Methods 0.000 claims 1
- 230000010349 pulsation Effects 0.000 claims 1
- 230000007017 scission Effects 0.000 claims 1
- 230000005284 excitation Effects 0.000 abstract description 4
- 239000013522 chelant Substances 0.000 abstract description 3
- 150000004697 chelate complex Chemical class 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 238000005259 measurement Methods 0.000 description 7
- 229910052747 lanthanoid Inorganic materials 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 210000002966 serum Anatomy 0.000 description 5
- 150000002602 lanthanoids Chemical class 0.000 description 4
- WVVLURYIQCXPIV-UHFFFAOYSA-N 4,4,4-trifluoro-1-naphthalen-2-ylbutane-1,3-dione Chemical compound C1=CC=CC2=CC(C(=O)CC(=O)C(F)(F)F)=CC=C21 WVVLURYIQCXPIV-UHFFFAOYSA-N 0.000 description 3
- ZMBHCYHQLYEYDV-UHFFFAOYSA-N trioctylphosphine oxide Chemical compound CCCCCCCCP(=O)(CCCCCCCC)CCCCCCCC ZMBHCYHQLYEYDV-UHFFFAOYSA-N 0.000 description 3
- 229910052693 Europium Inorganic materials 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- OGPBJKLSAFTDLK-UHFFFAOYSA-N europium atom Chemical compound [Eu] OGPBJKLSAFTDLK-UHFFFAOYSA-N 0.000 description 2
- 230000036046 immunoreaction Effects 0.000 description 2
- 125000001622 2-naphthyl group Chemical group [H]C1=C([H])C([H])=C2C([H])=C(*)C([H])=C([H])C2=C1[H] 0.000 description 1
- UYQMAGRFYJIJOQ-UHFFFAOYSA-N 4,4,4-trifluoro-1-naphthalen-1-ylbutane-1,3-dione Chemical compound C1=CC=C2C(C(=O)CC(=O)C(F)(F)F)=CC=CC2=C1 UYQMAGRFYJIJOQ-UHFFFAOYSA-N 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- DGEZNRSVGBDHLK-UHFFFAOYSA-N [1,10]phenanthroline Chemical compound C1=CN=C2C3=NC=CC=C3C=CC2=C1 DGEZNRSVGBDHLK-UHFFFAOYSA-N 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000000149 argon plasma sintering Methods 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 229940088623 biologically active substance Drugs 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- -1 lanthanide chelate complex Chemical class 0.000 description 1
- MPQXHAGKBWFSNV-UHFFFAOYSA-N oxidophosphanium Chemical class [PH3]=O MPQXHAGKBWFSNV-UHFFFAOYSA-N 0.000 description 1
- 150000003003 phosphines Chemical class 0.000 description 1
- LRWJZGCOPMDWFZ-UHFFFAOYSA-N phthalic acid;hydrochloride Chemical compound Cl.OC(=O)C1=CC=CC=C1C(O)=O LRWJZGCOPMDWFZ-UHFFFAOYSA-N 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000000941 radioactive substance Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
- G01N33/539—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody involving precipitating reagent, e.g. ammonium sulfate
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2458/00—Labels used in chemical analysis of biological material
- G01N2458/40—Rare earth chelates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/80—Fluorescent dyes, e.g. rhodamine
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/805—Optical property
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/825—Pretreatment for removal of interfering factors from sample
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Priority Applications (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE8102753A SE425439B (sv) | 1981-04-30 | 1981-04-30 | Immunologisk analys med lantanidkelatkomplex som markor |
| EP82850077A EP0064484B1 (de) | 1981-04-30 | 1982-04-08 | Methode zur fluoreszenz-spektroskopischen Bestimmung von biologisch aktiven Substanzen |
| EP85200392A EP0159066A1 (de) | 1981-04-30 | 1982-04-08 | Verfahren zur Trennung eines Lanthanid-Markierungsmittels von einer mit Lanthanid markierten immunochemischen Verbindung |
| AT82850077T ATE21173T1 (de) | 1981-04-30 | 1982-04-08 | Methode zur fluoreszenz-spektroskopischen bestimmung von biologisch aktiven substanzen. |
| DE8282850077T DE3272260D1 (en) | 1981-04-30 | 1982-04-08 | Method for fluorescence spectroscopic determination of a biologically active substance |
| JP57072480A JPS57186170A (en) | 1981-04-30 | 1982-04-28 | Fluorimetry method for biological substance |
| US06/513,744 US4565790A (en) | 1981-04-30 | 1983-07-14 | Method for fluorescence spectroscopic determination of a biologically active substance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE8102753A SE425439B (sv) | 1981-04-30 | 1981-04-30 | Immunologisk analys med lantanidkelatkomplex som markor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SE425439B true SE425439B (sv) | 1982-09-27 |
Family
ID=20343715
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE8102753A SE425439B (sv) | 1981-04-30 | 1981-04-30 | Immunologisk analys med lantanidkelatkomplex som markor |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US4565790A (de) |
| EP (2) | EP0064484B1 (de) |
| JP (1) | JPS57186170A (de) |
| AT (1) | ATE21173T1 (de) |
| DE (1) | DE3272260D1 (de) |
| SE (1) | SE425439B (de) |
Families Citing this family (41)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE8301395L (sv) * | 1983-03-15 | 1984-09-16 | Wallac Oy | Kelatiserande foreningar med funktionella grupper vilka tillater kovalent koppling till bio-organiska molekyler |
| US4629693A (en) * | 1984-03-27 | 1986-12-16 | Syntex (U.S.A.) Inc. | Sensitivity in fluorescence assays in icteric samples |
| US5830769A (en) * | 1985-03-18 | 1998-11-03 | Wieder; Irwin | Homogeneous fluorassay methods employing fluorescent background rejection and water-soluble rare earth metal chelates |
| US4761481A (en) * | 1985-03-18 | 1988-08-02 | Baxter Travenol Laboratories, Inc. | Substituted pyridine derivatives |
| SE8502573D0 (sv) * | 1985-05-23 | 1985-05-23 | Jouko Kanakre | Fluorescent lanthanide chelates useful as labels of physiologically active materials |
| IT1186733B (it) * | 1985-06-05 | 1987-12-16 | Eniricerche Spa | Composti tripeptidici ad azione ipotensiva |
| US5155216A (en) * | 1985-08-15 | 1992-10-13 | Amoco Corporation | Nucleic acids labeled with a chemiluminescent acridine ester |
| WO1987002708A1 (en) * | 1985-10-24 | 1987-05-07 | Siska Diagnostics, Inc. | Lanthanide chelate-tagged nucleic acid probes |
| US5585279A (en) * | 1986-01-23 | 1996-12-17 | Davidson; Robert S. | Time-resolved luminescence binding assays using a fluorescent transition metal label other than ruthenium |
| GB8601646D0 (en) * | 1986-01-23 | 1986-02-26 | Davidson R S | Binding assay & reagent |
| US4727024A (en) * | 1986-05-12 | 1988-02-23 | Martin Koocher | Binding assays involving formation and detection of light scattering crystals |
| WO1987007955A1 (en) | 1986-06-17 | 1987-12-30 | Baxter Travenol Laboratories, Inc. | Homogeneous fluoroassay methods employing fluorescent background rejection and water-soluble rare earth metal chelate fluorophores |
| US4925804A (en) * | 1986-06-17 | 1990-05-15 | Baxter International Inc. | Interligand metal transfer assay |
| GB8622855D0 (en) * | 1986-09-23 | 1986-10-29 | Ekins R P | Determining biological substance |
| SE8703682L (sv) * | 1987-09-24 | 1989-03-25 | Wallac Oy | Homogen bestaemningsmetod som utnyttjar affinitetsreaktioner |
| US4978625A (en) * | 1987-10-19 | 1990-12-18 | Becton, Dickinson And Company | Fluorescence immunoassay using water insoluble dyes |
| WO1989004375A1 (en) * | 1987-10-23 | 1989-05-18 | Siska Diagnostics, Inc. | Lanthanide chelate-tagged nucleic acid probes |
| EP0493745A1 (de) * | 1990-12-21 | 1992-07-08 | Dojindo Laboratories | Fluoreszierende Verbindung, Komplex, Reagenz und spezifische Bindungtest |
| FI88545C (fi) * | 1991-03-12 | 1993-05-25 | Jouko Kankare | Bestaemningsmetod |
| US5792330A (en) * | 1995-05-31 | 1998-08-11 | Board Of Regents, The University Of Texas System | Lanthanide metal cations for concurrent detection and separation in capillary electrophoresis |
| JP3538277B2 (ja) | 1996-03-08 | 2004-06-14 | 和子 松本 | 免疫測定用標識試薬及びそれに用いる蛍光性化合物及び錯体、及びそれらを用いる生物物質の免疫測定法 |
| US20050227294A1 (en) * | 1997-09-15 | 2005-10-13 | Molecular Devices Corporation | Molecular modification assays involving lipids |
| US7070921B2 (en) | 2000-04-28 | 2006-07-04 | Molecular Devices Corporation | Molecular modification assays |
| US7632651B2 (en) * | 1997-09-15 | 2009-12-15 | Mds Analytical Technologies (Us) Inc. | Molecular modification assays |
| US7745142B2 (en) * | 1997-09-15 | 2010-06-29 | Molecular Devices Corporation | Molecular modification assays |
| US6030840A (en) * | 1998-06-15 | 2000-02-29 | Nen Life Sciences, Inc. | Neutral enhancement of lanthanides for time resolved fluorescence |
| US6838292B1 (en) | 1999-04-19 | 2005-01-04 | Alion Science And Technology Corporation | Detection of biological warfare agents |
| US6653146B1 (en) | 1999-11-15 | 2003-11-25 | Chemclean Corporation | Bio-burden visualization system |
| JP4545337B2 (ja) * | 2001-03-23 | 2010-09-15 | オリンパス株式会社 | 顕微鏡 |
| WO2003003063A2 (en) * | 2001-06-28 | 2003-01-09 | Ia, Inc. | Fiber-optic sensor array |
| EP1437338B1 (de) * | 2001-10-10 | 2009-09-16 | Hitachi High-Technologies Corporation | Leuchtverbindungen und markierungsreagentien für die diese verwendet werden |
| US7211440B2 (en) * | 2002-03-08 | 2007-05-01 | Wallac Oy | Dissociative fluorescence enhancement assay |
| CA2581639C (en) * | 2004-09-30 | 2016-07-26 | Molecular Devices Corporation | Luminescent lanthanide complexes |
| US20060166376A1 (en) * | 2005-01-21 | 2006-07-27 | Craig Alan R | Compositions for use as a signal generation component and methods of using same |
| US7482444B2 (en) * | 2006-03-13 | 2009-01-27 | Wallac Oy | Terminating substrates for DNA polymerases |
| US8778614B2 (en) | 2010-08-24 | 2014-07-15 | Enzo Life Sciences, Inc. | Assays for detecting modified compounds |
| CN103969432B (zh) * | 2014-03-27 | 2016-02-17 | 中国科学院福建物质结构研究所 | 一种稀土纳米材料溶解增强时间分辨荧光免疫分析方法 |
| US9834808B2 (en) | 2016-01-21 | 2017-12-05 | SeLux Diagnostics, Inc. | Methods for rapid antibiotic susceptibility testing |
| ES2862345T3 (es) | 2016-01-21 | 2021-10-07 | Selux Diagnostics Inc | Métodos rápidos para probar la susceptibilidad antimicrobiana |
| JP7146765B2 (ja) | 2016-12-23 | 2022-10-04 | セルックス・ダイアグノスティクス・インコーポレイテッド | 改善された迅速な抗菌薬感受性試験のための方法 |
| WO2021041710A1 (en) | 2019-08-27 | 2021-03-04 | SeLux Diagnostics, Inc. | Systems and methods for performing antimicrobial susceptibility testing |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4341957A (en) * | 1975-11-26 | 1982-07-27 | Analytical Radiation Corporation | Fluorescent antibody composition for immunofluorometric assay |
| US4058732A (en) * | 1975-06-30 | 1977-11-15 | Analytical Radiation Corporation | Method and apparatus for improved analytical fluorescent spectroscopy |
| IL49685A (en) * | 1976-05-31 | 1978-10-31 | Technion Res & Dev Foundation | Specific binding assay method for determining the concentration of materials and reagent means therefor |
| US4283382A (en) * | 1977-12-28 | 1981-08-11 | Eastman Kodak Company | Fluorescent labels comprising rare earth chelates |
| US4259313A (en) * | 1978-10-18 | 1981-03-31 | Eastman Kodak Company | Fluorescent labels |
-
1981
- 1981-04-30 SE SE8102753A patent/SE425439B/sv not_active IP Right Cessation
-
1982
- 1982-04-08 EP EP82850077A patent/EP0064484B1/de not_active Expired
- 1982-04-08 AT AT82850077T patent/ATE21173T1/de not_active IP Right Cessation
- 1982-04-08 EP EP85200392A patent/EP0159066A1/de not_active Ceased
- 1982-04-08 DE DE8282850077T patent/DE3272260D1/de not_active Expired
- 1982-04-28 JP JP57072480A patent/JPS57186170A/ja active Granted
-
1983
- 1983-07-14 US US06/513,744 patent/US4565790A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH049263B2 (de) | 1992-02-19 |
| EP0064484A2 (de) | 1982-11-10 |
| EP0064484A3 (en) | 1983-01-19 |
| DE3272260D1 (en) | 1986-09-04 |
| US4565790A (en) | 1986-01-21 |
| JPS57186170A (en) | 1982-11-16 |
| EP0064484B1 (de) | 1986-07-30 |
| ATE21173T1 (de) | 1986-08-15 |
| EP0159066A1 (de) | 1985-10-23 |
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