RU96123719A - RECONSTRUCTED WORK - Google Patents
RECONSTRUCTED WORKInfo
- Publication number
- RU96123719A RU96123719A RU96123719/13A RU96123719A RU96123719A RU 96123719 A RU96123719 A RU 96123719A RU 96123719/13 A RU96123719/13 A RU 96123719/13A RU 96123719 A RU96123719 A RU 96123719A RU 96123719 A RU96123719 A RU 96123719A
- Authority
- RU
- Russia
- Prior art keywords
- interferon
- dna fragment
- human immune
- recombinant human
- encoding
- Prior art date
Links
- 102000008070 Interferon-gamma Human genes 0.000 claims 10
- 108010074328 Interferon-gamma Proteins 0.000 claims 10
- 229920003013 deoxyribonucleic acid Polymers 0.000 claims 7
- 229940044627 gamma-interferon Drugs 0.000 claims 6
- XSQUKJJJFZCRTK-UHFFFAOYSA-N urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims 4
- QIVBCDIJIAJPQS-SECBINFHSA-N D-tryptophane Chemical compound C1=CC=C2C(C[C@@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-SECBINFHSA-N 0.000 claims 2
- 241000588724 Escherichia coli Species 0.000 claims 2
- 239000004202 carbamide Substances 0.000 claims 2
- 235000015097 nutrients Nutrition 0.000 claims 2
- 102000037197 Anion exchangers Human genes 0.000 claims 1
- 108091006437 Anion exchangers Proteins 0.000 claims 1
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 claims 1
- 238000005273 aeration Methods 0.000 claims 1
- 150000001450 anions Chemical class 0.000 claims 1
- 230000001580 bacterial Effects 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 238000004587 chromatography analysis Methods 0.000 claims 1
- 239000012535 impurity Substances 0.000 claims 1
- 150000007522 mineralic acids Chemical class 0.000 claims 1
- 150000007524 organic acids Chemical class 0.000 claims 1
- 239000008363 phosphate buffer Substances 0.000 claims 1
- 238000004153 renaturation Methods 0.000 claims 1
- 238000004007 reversed phase HPLC Methods 0.000 claims 1
- 150000003839 salts Chemical class 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 claims 1
- 230000002194 synthesizing Effects 0.000 claims 1
- 230000035897 transcription Effects 0.000 claims 1
Claims (4)
5'GAATTCAGGAGGAATCTAGA3'
3'CTTAAGTCCTCCTTAGATCT5'
XbaI-BamHI фрагмент ДНК размером 450 п.о., кодирующий ген рекомбинантного иммунного интерферона человека, BamHI-ScaI фрагмент ДНК размером 850 п.о. плазмиды рКК233-3, содержащий последовательность терминатора транскрипции rrnВТТ, PstI-PstI фрагмент ДНК размером 1500 п.о., кодирующий ген kan плазмиды pUC4K; BglI-HindIII фрагмент ДНК размером 1400 п.о. плазмиды pUC19.1. Recombinant plasmid DNA pTTg Km2, encoding the synthesis of recombinant human immune interferon, 4440 base pairs (p.o.), containing the HindIII-EcoRI 220-bp DNA fragment containing the sequence of the tryptophan promoter; EcoRI-XbaI DNA fragment size of 20 p. O., containing a synthetic ribosome binding site:
5'GAATTCAGGAGGAATCTAGA3 '
3'CTTAAGTCCTCCTTAGATCT5 '
The XbaI-BamHI DNA fragment of 450 p. O., encoding the gene of recombinant human immune interferon, the BamHI-ScaI DNA fragment of 850 p. pKK233-3 plasmid containing the rrnBTT transcription terminator sequence, PstI-PstI DNA fragment of 1500 bp, encoding the kan gene of the plasmid pUC4K; BglI-HindIII DNA fragment size 1400 p. plasmids pUC19.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RU96123719/13A RU2097428C1 (en) | 1996-12-23 | 1996-12-23 | Recombinant plasmid dna pttg km2 encoding synthesis of recombinant human immune interferon, strain escherichia coli t3g - a producer of recombinant human immune interferon and a method of preparing recombinant human immune interferon |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RU96123719/13A RU2097428C1 (en) | 1996-12-23 | 1996-12-23 | Recombinant plasmid dna pttg km2 encoding synthesis of recombinant human immune interferon, strain escherichia coli t3g - a producer of recombinant human immune interferon and a method of preparing recombinant human immune interferon |
Publications (2)
Publication Number | Publication Date |
---|---|
RU2097428C1 RU2097428C1 (en) | 1997-11-27 |
RU96123719A true RU96123719A (en) | 1998-03-20 |
Family
ID=20188215
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
RU96123719/13A RU2097428C1 (en) | 1996-12-23 | 1996-12-23 | Recombinant plasmid dna pttg km2 encoding synthesis of recombinant human immune interferon, strain escherichia coli t3g - a producer of recombinant human immune interferon and a method of preparing recombinant human immune interferon |
Country Status (1)
Country | Link |
---|---|
RU (1) | RU2097428C1 (en) |
-
1996
- 1996-12-23 RU RU96123719/13A patent/RU2097428C1/en not_active IP Right Cessation
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