RU70580U1 - MULTI-FUNCTIONAL AUTOMATIC BIOSENSOR ANALYZER OF ORGANIC COMPOUNDS - Google Patents

Abstract

The utility model relates to the field of determining the content of organic substances using the interaction of organic substances with biological material and recording changes in electric current in the zone of this interaction and can be used for rapid assessment of the content of organic compounds in aqueous media of effluents of chemical, light, food, metallurgical enterprises and engineering industries. Technical result achieved: - expanding the range of defined organic compounds; - ensuring the determination of organic compounds in opaque and heterogeneous aqueous media. The essence of the utility model is as follows. The multifunctional automatic biosensor analyzer of organic compounds contains a housing (1), a sampling device (2) located in it, connected to a sensitive element (3), a flow rate analyzer of the analyzed compounds (4), an electronics unit (5) connected to the sensitive element, and an output recorder signal (6) and with a stimulator of the consumption of the analyzed compounds. The sensitive element is made in the form of four replaceable Clark electrodes (7) with immobilized biological materials (8), oxidizing organic compounds with oxygen absorption, placed in a common flow cell (9), connected to a sampling device and with a flow inducer of the analyzed organic compounds. In this case, the electronics unit has four channels for amplifying and processing electric signals (10), each of which is connected to the corresponding Clark electrode and to the output signal recorder.

Description

The utility model relates to the field of determining the content of organic substances using the interaction of organic substances with biological material and recording changes in electric current in the zone of this interaction and can be used for rapid assessment of the content of organic compounds in aqueous media of effluents of chemical, light, food, metallurgical enterprises and engineering industries.

Known automatic analyzer for the detection of toxic impurities, containing a housing, indicator means, a mechanism for supplying an indicator means, a system for supplying analytes to the reaction zone, a photoblock, an electronic measuring unit and an output signal recorder, see USSR author's certificate No. 11255515, class G01N 21/78 published 11/23/1984 in the ballot No. 43. In it, an indicator tablet is used as an indicator means, which is formed from an indicator powder in a powder chamber and fed into the reaction chamber and after analysis is returned to the powder chamber and mixed with the remaining mass of the indicator powder.

A disadvantage of the known analyzer is the low sensitivity of detection of toxic impurities, the low range of detectable toxic impurities, the decrease in sensitivity over time due to mixing of the reacted indicator powder with its bulk in the powder chamber, in which a new indicator tablet is formed in preparation for analysis.

Also known is a multifunctional automatic biosensor analyzer of organic compounds containing a housing located in

it is a sampling device connected to a sensitive element, a flow rate analyzer of the analyzed compounds, an electronics unit connected to a sensitive element, an output signal recorder and a flow rate of the analyzed compounds, which is used to analyze toxic media, see utility model according to patent No. 47100 class G01N 21 / 00, published in bulletin No. 22 dated 08/10/2005. The sensitive element is formed by an indicator tape with a sample of the analyzed organic compounds taken, to which ikatornye reagents (enzyme buffer solution - e.g., horse cholinesterase, and indicator solution) and photoblock converting the change in optical density of the indicator tape to an electrical signal.

The biological material is equine cholinesterase, in the interaction of which with organic compounds its inhibition occurs, leading to a change in the optical density of the indicator tape and a change in the magnitude of the corresponding electrical signal.

A disadvantage of the known analyzer are:

- a narrow range of defined organic compounds;

- the inability to determine organic compounds in opaque and heterogeneous aqueous media.

In this utility model, the technical task is posed:

- expanding the range of defined organic compounds;

- ensuring the determination of organic compounds in opaque and heterogeneous aqueous media.

The solution to this problem is provided due to the fact that in the multifunctional automatic biosensor analyzer of organic compounds containing a housing, a sampling device located in it connected to a sensitive element, a flow rate stimulator of the analyzed compounds, an electronics unit,

connected to a sensitive element, an output signal recorder, and a flow rate stimulator of the analyzed compounds, the sensitive element is made in the form of four replaceable Clark electrodes with immobilized biological materials that oxidize organic compounds with oxygen absorption, placed in a common flow cell connected to a sampling device and with a flow stimulator analyzed organic compounds, while the electronics unit has four channels of amplification and processing of electrical signals Each of which is connected to a respective electrode and Clark recorder output.

The claimed multifunctional automatic biosensor analyzer of organic compounds has a combination of essential features unknown from the prior art for devices of this purpose, which allows us to conclude that the criterion of "novelty" for a utility model is met.

The device of the utility model is illustrated using the drawings, which depict:

figure 1 - functional diagram of the analyzer.

The device of the utility model is as follows.

The multifunctional automatic biosensor analyzer of organic compounds contains a housing (1), a sampling device (2) located in it, connected to a sensitive element (3), a flow rate analyzer of the analyzed compounds (4), an electronics unit (5) connected to the sensitive element, and an output recorder signal (6) and with a stimulator of the consumption of the analyzed compounds.

The sensitive element is made in the form of four replaceable Clark electrodes (7) with immobilized biological materials (8), oxidizing organic compounds with oxygen absorption, placed in a common flow cell (9) connected to a sampling probe

device and with a stimulator of the consumption of the analyzed organic compounds. In this case, the electronics unit has four channels for amplifying and processing electric signals (10), each of which is connected to the corresponding Clark electrode and to the output signal recorder.

As a biological material, various microorganisms are used in Clark's electrodes, for example, yeast, which oxidize various analyzed organic compounds.

The analyzer has a controllable hydraulic distributor (11) connected to a sampling device and a container (12) with a reference test solution, while a sensitive element can be automatically connected through a hydraulic distributor either to a container with a reference test solution or to a sampling device.

The work of a multifunctional automatic biosensor analyzer is as follows.

At the beginning, the hydraulic distributor (11) is installed in the first position at which the reference test solution is sucked by a flow inducer through the flow cell (9) and the analyzer is checked for operability.

Then, the hydraulic distributor (11) is installed in the second position, in which the analyzed sample of the aqueous medium is supplied through the sampling device (2) to the flow cell (9) and the biological material (8) interacts with the organic compounds present in the aqueous medium. In this case, the biological material absorbs oxygen, decomposing organic compounds and changes the magnitude of the electrical signals recorded from each electrode (7). Amplified and transformed by each channel (10) of the electronics unit (5), the signals arrive at the recorder (6) and characterize the value

biological oxygen consumption, which determines the content of various organic compounds in opaque and heterogeneous aqueous media.

The proposed analyzer can be repeatedly made from elements produced by the domestic industry and can be used in industrial enterprises and the environmental service to determine the content of organic compounds in transparent, opaque and heterogeneous aqueous media of industrial effluents, which meets the criterion of "industrial applicability" for a utility model.

The applicant has developed and manufactured a prototype of the proposed analyzer, which was tested with a positive result and is implemented for the rapid assessment of the content of organic compounds in transparent, opaque, heterogeneous aqueous media of industrial wastewater.

Claims (1)

A multifunctional automatic biosensor analyzer of organic compounds, comprising a housing, a sampling device located therein, connected to a sensing element, an analyzer connected to a sensor, an output unit connected to a sensor, an output signal recorder and an analyzer connected to an analyzer, characterized in that the sensor made in the form of four replaceable Clark electrodes with immobilized biological materials that oxidize or oxygen absorbing compounds placed in a common flow cell connected to a sampling device and with a flow inducer of the analyzed organic compounds, while the electronics block has four channels for amplifying and processing electrical signals, each of which is connected to the corresponding Clark electrode and to the output signal recorder .