RU59255U1 - ORGANIC / TOXIC COMPOSITION BIOSYENSER AUTOMATIC ANALYZER - Google Patents

Abstract

The utility model relates to the field of determining the content of organic substances using the interaction of organic substances with biological material and detecting changes in electric current in the zone of this interaction and can be used for rapid assessment of the content of organic / toxic compounds in aqueous media. Technical result achieved: - expanding the range of defined organic compounds; - providing an analyzer health check. The essence of the utility model is as follows. A biosensor automatic analyzer of organic / toxic compounds, containing a housing (1), a sampling device (2) located in it, connected to a sensitive element (3), a flow inducer of the analyzed compounds (4), an electronics unit (5) connected to the sensitive element, an output signal recorder (6) and with a flow inducer of the analyzed compounds. The sensitive element (3) is made in the form of a flow cell with an electrode (7), on which biological material (8) is deposited, decomposing organic toxic compounds with oxygen absorption. In this case, the flow cell is connected to the output of the hydraulic distributor (9), one of the inputs of which is connected to a sampling device (2), and the other to a container with a reference test solution (10).

Description

The utility model relates to the field of determining the content of organic substances using the interaction of organic substances with biological material and detecting changes in electric current in the zone of this interaction and can be used for rapid assessment of the content of organic / toxic compounds in aqueous media.

Known automatic analyzer for the detection of toxic impurities, containing a housing, indicator means, a mechanism for supplying an indicator means, a system for supplying analytes to the reaction zone, a photoblock, an electronic measuring unit and an output signal recorder, see USSR copyright certificate No. 1125515, class G 01 N 21 / 78, published on November 23, 1984, in Bulletin No. 43. In it, an indicator tablet is used as an indicator means, which is formed from an indicator powder in a powder chamber and fed into the reaction chamber and after analysis is returned to the powder chamber and mixed with the remaining mass of the indicator powder.

A disadvantage of the known analyzer is the low sensitivity of detection of toxic impurities, the low range of detectable toxic impurities, the decrease in sensitivity over time due to mixing of the reacted indicator powder with its bulk in the powder chamber, in which a new indicator tablet is formed in preparation for analysis.

Also known is a biosensor automatic analyzer of organic toxic compounds, comprising a housing, a sampling device located therein, connected to a sensing element,

stimulator of the consumption of the analyzed compounds, see utility model according to patent No. 47100 class G 01 N 21/00, published in the bulletin No. 22 dated 08/10/2005. The sensitive element is formed by an indicator tape with a sample of the analyzed organic / toxic compounds, to which the indicator reagents (buffer solution of the enzyme - for example, horse cholinesterase, and indicator solution) and a photoblock that converts the change in the optical density of the indicator tape into an electrical signal.

The biosensor is an enzyme - equine cholinesterase, in the interaction of which with organic / toxic compounds its inhibition occurs leading to a change in the optical density of the indicator tape, and a change in the magnitude of the corresponding electrical signal.

A disadvantage of the known analyzer are:

- a narrow range of detectable organic / toxic compounds;

- the inability to verify the performance of the analyzer in the analysis of aqueous media.

In this utility model, the technical task is posed:

- expanding the range of defined organic compounds;

- providing a test of the performance of the analyzer in the analysis of aqueous media.

The solution to this problem is as follows.

In a biosensor automatic analyzer of organic / toxic compounds containing a housing, a sampling device located in it, connected to a sensitive element, a flow inducer of the analyzed compounds, an electronics unit connected to a sensitive element, an output signal recorder and a flow stimulator of the analyzed compounds, the sensitive element is made in the form of a flow cell with an electrode on which biological material is deposited that decomposes organic toxic compounds scheniem oxygen. In this case, the flow cell is connected to the outlet

a hydraulic distributor, one of the inputs of which is connected to a sampling device, and the other to a container with a reference test solution.

The claimed biosensor automatic analyzer of organic / / toxic compounds has a combination of essential features unknown from the prior art for devices of this purpose, which allows us to conclude that the criterion of "novelty" for a utility model is met.

The device of the utility model is illustrated using the drawings, which depict:

figure 1 - functional diagram of the analyzer.

The device of the utility model is as follows.

A biosensor automatic analyzer of organic / toxic compounds, containing a housing (1), a sampling device (2) located in it, connected to a sensitive element (3), a flow inducer of the analyzed compounds (4), an electronics unit (5) connected to the sensitive element, an output signal recorder (6) and with a flow inducer of the analyzed compounds.

The sensitive element (3) is made in the form of a flow cell with an electrode (7), on which biological material (8) is deposited, decomposing organic toxic compounds with oxygen absorption. In this case, the flow cell is connected to the output of the hydraulic distributor (9), one of the inputs of which is connected to a sampling device (2), and the other to a container with a reference test solution (10).

The sampling device (2) is made in the form of a remote pipeline with a filter to prevent solid particles, dirt, etc. from entering the analyzer.

The electrode (7) is a Clark electrode. Immobilized cells of microorganisms, for example, yeast, are used as biological material (8).

The electronics unit (5) serves to amplify and convert the electric signal from the electrode (7), supply the signal to the recorder (6) and to control the operation of the analyzer.

The hydraulic distributor (9) has two positions. In the first position, a container with a reference test solution is connected to the flow cell. In the second position, a sampling device is connected to the flow cell.

The biosensor analyzer is as follows.

At the beginning, the hydraulic distributor is installed in the first position at which the reference test solution is pumped by the flow stimulator (4) through the flow cell (3) and the analyzer is checked for operability.

Then, the hydraulic distributor (9) is installed in the second position in which the analyzed sample of the aqueous medium is supplied through the sampling device (2) to the flow cell (3) and the biological material (8) interacts with the organic / toxic compounds present in the aqueous medium. In this case, the biological material absorbs oxygen, decomposing organic / toxic compounds and changes the magnitude of the electric signal taken from the electrode (7). The signal amplified and transformed by the electronics unit (5) enters the recorder (6) and characterizes the value of the biological oxygen consumption, which determines the content of organic / toxic compounds in aqueous media.

The proposed analyzer can be repeatedly made from elements produced by the domestic industry and can be used in industrial enterprises and the environmental service to determine the content of organic / toxic aqueous media and industrial effluents, which meets the criterion of "industrial applicability" for utility models.

The applicant has developed and manufactured a prototype of the proposed analyzer, which is tested with a positive result.

Claims (1)

A biosensor automatic analyzer of organic toxic compounds, comprising a housing, a sampling device located therein, connected to a sensing element, an analyzer connected to a sensor, an output unit and an analyzer of output signal, and an analyzer connected to an analyzer, characterized in that the sensor made in the form of a flow cell with two electrodes, on one of the electrodes of which a biosensor material is deposited, which decompose organic toxic compounds with oxygen absorption, while the flow cell is connected to the output of the hydraulic distributor, one of the inputs of which is connected to a sampling device, and the other to a container with a reference test solution.