RU2803692C1 - Method of treating gum recession using gingival tissue and regeneration stimulant preparation - Google Patents

Abstract

FIELD: medicine; therapeutic dentistry.

SUBSTANCE: invention can be used in the treatment of pathological conditions such as gingival recession. An injection of 0.1 ml of a medicinal mixture containing Alloplant, a powdered regeneration stimulator which is pre-soaked in a 0.9% sodium chloride solution in a mass ratio of 1:1 is performed into the gum mucosa, and as a second component, an autosuspension of human gums in a mass ratio components 1:1 is injected as a course of 1 procedure. The next day, the mouth is rinsed with Erakond, diluted with water in a 1:1 ratio, in a course of 5 procedures, carried out every other day.

EFFECT: invention provides enhanced regeneration of gingival tissue.

1 cl, 1 ex

Description

The invention relates to the field of medicine, namely to therapeutic dentistry, and can be used in the treatment of pathological conditions such as Miller class 1 and 2 gingival recessions associated with the fine structure of periodontal tissues (biotype).

The process of development of gingival recession not only leads to the formation of severe aesthetic defects, but sooner or later ends in tooth loss due to loss of stability with the development of overload and atrophy, and the formation of a periodontal pocket in the recession zone.

There are quite a large number of methods for surgical treatment of recessions.

There is a known method of surgical treatment of gum recession [RF patent No. 2301030, IPC A61B 17/24, publ. 06/20/2007], which consists in making a semilunar incision in the area of gum recession to the bone. The semilunar flap is removed and moved coronally. The vestibule of the oral cavity is deepened by detaching the mucous membrane, submucosal cords and muscle fibers in the donor area. A fibrin membrane containing platelet-derived growth factors is placed in the resulting space between the semilunar flap and the mobile mucosa.

It is also known to use Silverstein LH, Callan D. AlloDerm ® - An Acellular Dermal Matrix Allograft Substitute for Soft Tissue Grafting instead of an autograft. CompendContEd - PostGraduateSeries 3(4): 14-21. 1996.] as a non-invasive way to increase the volume of soft tissue in the area of recession.

There is a known method of treating gum recession, which consists in the use of mesenchymal multipotent stem cells from rat adipose tissue and sterile bioplastic collagen material “Collost” gel 7%. In 0.1 ml of the mixture, 1 laboratory animal was injected with 100 thousand mesenchymal stem cells of adipose tissue [Rubnikovich S.P., Denisova Yu.L., Andreeva V.A., Kvacheva Z.B., Panasenkova G.Yu., Khomich I.S. Evaluation of the effectiveness of the complex use of mesenchymal stem cells and bioplastic material COLLOST on a model of gingival recession in an in vivo experiment. Russian dentistry. 2018; 11(3):35-44]. The disadvantages of the method are the high cost of the drug used, Collost" gel 7%, and allergic reactions to this drug.

There is a known method for treating gum recession, taken as a prototype, which involves isolating multipotent mesenchymal stem cells (MMSC) from rat adipose tissue. Then alloplant regeneration stimulator powder in an amount of 0.1 g is pre-soaked in a 0.9% sodium chloride solution in a mass ratio of 1:1, then mixed with 150 thousand MMSC cells. After this, the mixture is injected into the gum mucosa in the area of the lower incisors. Then SANS MOTS oil in an amount of 0.1 ml is applied to the area of gum recession as an application for 20 minutes [RF patent No. 2785189, 2022]. The disadvantage of the prototype is the labor-intensive and time-consuming process of isolating multipotent stem cells and culturing them in laboratory conditions.

The search for effective methods for treating periodontal diseases is currently a pressing problem in modern dentistry. On the one hand, this is due to the still high prevalence of this disease among the population, including young people, and on the other hand, due to the insufficient effectiveness of the proposed modern means and methods of treatment.

The objective of the invention is to expand the arsenal of methods for treating gum recession.

The technical result when using the invention is to simplify the method and enhance the regeneration of gingival tissue.

The proposed method of treating gum recession is as follows.

In humans, under sterile conditions, the hypertrophied gingival papilla is excised in an amount of 1 g. Then, using scissors and tweezers, under sterile conditions, the resulting mass is cut into small pieces, and placed in a sterile centrifuge tube with 1.5 ml physiological solution, centrifuged at 1500 vol. /min, for 3 minutes. Then the excess liquid is drained, fresh saline solution (5 ml) is added, the pieces are shaken and a suspension is obtained. Transfer the suspension in portions to a sterile 100-μm nylon sieve mounted on a sterile centrifuge tube. Using the plunger of a 10 ml syringe, rub the pieces of fabric through a sieve. Wash the sieve with 5 ml of saline solution and remove large fragments. Centrifuge at 1500 rpm for 5 minutes. The liquid is carefully drained. In the second stage, the sediment obtained as a result of centrifugation is mixed in a mass ratio of 1:1 with Alloplant powdered regeneration stimulator, which in an amount of 1 g is pre-soaked in a 0.9% sodium chloride solution in a mass ratio of 1:1. The patient is injected into the gum mucosa in the area of the defect in an amount of 0.1 ml. The course of treatment is one procedure. The next day, rinse the mouth with Erakond, diluted with water in a 1:1 ratio, which is repeated every other day, for a course of 5 procedures.

Erakond is a condensed plant extract of alfalfa (Medicago sativa), obtained from the ground part of the plant by hydrobarometric extraction, contains a multivitamin complex (vit. A, E, B ₆ , K), 8 essential amino acids, macro- and microelements (iron, potassium, phosphorus, magnesium, manganese, sulfur, potassium, silicon, fluorine, zinc, copper), carbohydrates, monosaccharides, uronic acids, flavonoids. Dietary supplement, additional source of iron, zinc and flavonoids.

Erakond has a pronounced modulating effect on all parts of the immune system due to: stimulating the production of precursors and enhancing the activity of mature immune cells; mobilization of lymphocytes from the depot, stabilization of myelo- and erythropoiesis; antioxidants that have a membrane-stabilizing effect (restore the cell membrane); radioprotectors (humic substances) having an ion-sorbent effect, binding radionuclides and heavy metal ions; flavonoids with hepatoprotective effects; saponins with adaptogenic (anti-stress) activity, stimulating nonspecific immunity.

Erakond has: a detoxifying effect, neutralizing a variety of toxins (man-made, environmental, medicinal, household, including ethanol); anti-inflammatory effect, by suppressing the excessive release of cytokines and reducing the hyperproduction of antibodies, reducing the sensitization of polymorphonuclear leukocytes during HRT and HNT, eliminates the processes of allergization and autointoxication that occur when the body’s own tissues are destroyed during the disease process; moderate antimicrobial and pronounced antifungal effect; wound healing effect for injuries, burns, chronic diseases of the skin (neurodermatitis, eczema, psoriasis) and mucous membranes (stomatitis, periodontal disease, pharyngitis, gastritis, peptic ulcer, colitis and others); the ability to stimulate the adrenal glands, improves the structure of bone tissue in osteoporosis, diseases of the joints and spine, accelerates the healing of bone fractures.

Alloplant is a biomaterial for regenerative surgery, which has extremely low antigenic properties, practically eliminating the immune nature of the reaction after its implantation [RF patent 2189257, 2002].

Using the proposed method, a group of 20 patients diagnosed with gum recession was treated.

Evaluation of results.

On the 2nd day, the general condition of the patients was satisfactory. Gum recession remained and amounted to 2.8±0.02 mm, hyperemia was also noted on the lower jaw, the gums were quite dense with a smooth edge. There was fibrinous plaque and bleeding of the gums, and there was also a slight restoration of the tissue of the periodontal papilla

On the 5th day, the general condition was satisfactory. The recession of the gums remained and amounted to 2.5±0.02 mm, slight hyperemia, the gums were quite dense with a smooth edge. There is no plaque or bleeding of the gums, and there is a noticeable restoration of the tissue of the periodontal papilla.

On the 7th day, the general condition was satisfactory. The gum recession was 1.9±0.01 mm, slight hyperemia, the gums were quite dense with a smooth edge. There is no plaque or bleeding of the gums, and the tissue of the periodontal papilla has been completely restored.

After 3 months: general condition is satisfactory. There is no plaque or bleeding of the gums. The same level of the gingival margin was noted, and the dentogingival papilla was restored.

After 6 months: general condition is satisfactory. There is no plaque or bleeding of the gums. The same level of the gingival margin was noted, and the dentogingival papilla was restored. The gums are pink, dense, with smooth edges, both on the upper and lower jaws. There is also a roller-like thickening along the gingival margin in the injection area.

The essence of the invention is illustrated by the following example.

Patient A. was admitted with a diagnosis of gum recession in the area of tooth 1.3.

The hypertrophied gingival papilla in a volume of 1 g was excised from the patient under sterile conditions. Then, using scissors and tweezers, under sterile conditions, the resulting mass is cut into small pieces, and placed in a sterile centrifuge tube with 1.5 ml saline solution, centrifuged at 1500 vol. /min., for 3 minutes. After which, excess liquid is drained, fresh saline solution, 5 ml is added, the pieces are shaken and a suspension is obtained. Transfer the suspension in portions to a sterile 100-μm nylon sieve mounted on a sterile centrifuge tube. Using the plunger of a 10 ml syringe, rub the pieces of fabric through a sieve. Wash the sieve with 5 ml of saline solution and remove large fragments. Centrifuge at 1500 rpm for 5 minutes. The liquid is carefully drained. In the second stage, the sediment obtained as a result of centrifugation is mixed in a mass ratio of 1:1 with Alloplant powdered regeneration stimulator, which in an amount of 1 g is pre-soaked in a 0.9% sodium chloride solution in a mass ratio of 1:1. The patient is injected with a mixture in a volume of 0.1 ml into the gum mucosa in the area of the defect. The next day, rinse the mouth with Erakond, diluted with water in a 1:1 ratio, which is repeated every other day, for a course of 5 procedures.

Claims (1)

A method of treating gum recession, including injection into the gum mucosa of a therapeutic mixture containing Alloplant, a powdered regeneration stimulator, which is pre-soaked in a 0.9% sodium chloride solution in a mass ratio of 1:1, in a course of 1 procedure, characterized in that 0.1 is administered ml of a mixture containing autosuspension of human gums as a second component with a mass ratio of components of 1:1, the next day the mouth is rinsed with Erakond, diluted with water in a ratio of 1:1, in a course of 5 procedures, carried out every other day.