RU2800585C1 - Method for treatment of bordetella pneumonia in piglets - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: invention is intended for the treatment of bordetella pneumonia in piglets. Farmazin and oleandomycin are administered intramuscularly in doses of 5 mg/kg of body weight of each drug to piglets from 2 weeks to 4 months of age for 7 days.

EFFECT: invention provides an increase in the effectiveness of treatment of piglets with bordetella pneumonia.

1 cl, 3 tbl

Description

The invention relates to the field of veterinary microbiology and animal health and can be used for the treatment of bordetella pneumonia in piglets.

Pneumonia in piglets is caused by various microorganisms: Pasteurella, Salmonella, Escherichia, Klebsiella, Moraxella, Proteus, Citrobacter, Mycoplasma, Streptococcus, Staphylococcus, etc. At the same time, bordetella pneumonia in piglets is widespread.

There are various methods used to treat animals with pneumonia using: tetracycline, oxamycin, macrolides and fluoroquinolones, as well as lincomycin, kitasamycin (Materials of the conference "Problems of agricultural production at the present stage and ways to solve them." - Belgorod, 2005, p. 87 ). However, porcine pneumonia pathogens often develop resistance to chemotherapy drugs.

A method of treatment using fradisin and tylosin tartrate has a certain effectiveness in pneumonia of piglets (1).

There is also known a method for the treatment of pneumonia in piglets using tylosin tartrate, compared with fradisin, which is characterized by a higher efficiency of its use in diseases caused by gram-positive and gram-negative microflora (2).

Treatment of pneumonia in piglets using tylosin-containing drugs has a narrow spectrum of activity against microorganisms that cause pneumonia in piglets, and the rapid emergence of resistance in pathogens to them.

The objective of our research was to increase the effectiveness of treatment of piglets with pneumonia with bordetella pneumonia. However, in a patent search for methods of treating bordetella pneumonia in piglets using a composition of farmazin and oleandomycin, it was not found.

Therefore, it seems promising to create a method for the treatment of bordetella pneumonia in piglets using composite antibacterial drugs with a wide spectrum of action, such as farmazin and oleandomycin. In addition, the use of a method for the treatment of bordetella pneumonia in piglets with a composition of farmazin with oleandomycin expands the spectrum of antibacterial action of farmazin and reduces the toxic effect of the components of the composition.

Tylosin-containing drugs, which include farmazin, have high antimicrobial activity against gram-positive and relatively low gram-negative microflora.

Oleandomycin exhibits a wide range of antimicrobial activity, but has a certain toxicity.

Conducted laboratory studies make it possible to determine the optimal ratios of components in the created composition when studying their antimicrobial action against bacteria that play an etiological role in the occurrence of bordetella pneumonia in piglets.

The synergistic combination of farmazin with oleandomycin in a ratio of 1:1 is effective in the treatment of bordetella pneumonia in piglets. This combination is effective for the treatment of bordetella pneumonia with the appointment of farmazin and oleandomycin at a dose of 5 mg/kg of each of them per 1 kg of body weight for 7 days.

Specific examples of the proposed treatment method are shown in Table 1.

As the table data show, the most optimal proportion of the components of the composite preparation used in the proposed method of treating pneumonia in piglets is the ratio of farmazin and oleandomycin 1:1.

Table 1.

Development of the optimal proportion of the created composition farmazin plus oleandomycin

A drug Type of microorganisms -Bordeiella bronchisep[icum Minimum bacteriostatic concentration, mcg/ml Farmazina 31.00±4.03 Oleandomyce 21.50±0.05 Composition of farmazin with oleandomycin 1:1 7.53±1.01 2:1 12.25±2.01 3:1 15.87±2.01 Fractional inhibitory concentration (glkg/ml) at a ratio of 1:1 Farmazin 0.1 Oleandomycin 0.1 FIC connection index 0.2

The fractional inhibitory concentration of farmazin against Bordetella bronchisepticum when interacting with oleandomycin was 0.1, and oleandomycin when interacting with farmazin was 0.1. The fractional inhibitory index for the composition is 0.2, which predetermines the prospects for using this composition for the treatment of bordetella pneumonia in piglets.

Conducted laboratory studies stated a decrease in the rate of adaptation of microorganisms - the causative agents of pneumonia to the component of the composite preparation - farmazin when used together with oleandomycin (table 2).

table 2

Adaptation of Farmazin plus Oleandomycin Composition to Bordetella bronchiseptica

Farmazine background Background for composite preparation (1:1) Farmazin (after 30 passages) Composition of farmazin and oleandomycin drug (1:1),
(after 30 passages) Bordetella bronchisepticum Bordetella bronchisepticum Bordetella bronchisepticum Bordetella bronchisepticum 31.00±4.03 7.53±1.01 35.00±0.05 7.53±1.01

The results of the experiment on studying the therapeutic effect of the method using the composition of farmazin and oleandomycin 10 mg/kg (5 mg/kg farmazin plus 5 mg/kg oleandomycin) per kilogram of body weight of piglets (the animals received the drug intramuscularly for 7 days) are presented in table 3 .

Table 3

Comparative effectiveness of different methods of treatment of pneumonia in piglets

group of animals Composite preparation Positive control * Farmazin control Farmazin Oleandomycin The number of sick piglets at the beginning of the experiment, goal. 50 50 50 50 Recovered piglets, goal. 45 43 49 35 Therapeutic efficacy, % 90.0 86 82 70

The data obtained indicate that the therapeutic efficacy of the composition increases due to the synergistic interaction of farmazin and oleandomycin against the causative agent of Bordetella pneumonia in piglets - Bordetella bronchiseptica.

The proposed method is effective for piglets from 2 weeks to 4 months of age at doses of 5 mg/kg of body weight of each of the ingredients of the created composition (farmazin and oleandomycin) for 7 days in the treatment of bordetella pneumonia. The use of the method of treatment begins with the appearance of clinical signs of bordetella pneumonia.

The solution of the problem allows:

- to expand the spectrum of antimicrobial activity of drugs used in methods for the prevention of severe forms of bordetella pneumonia in piglets;

- to prevent the development of toxic effects by achieving a faster and more complete effect with the simultaneous administration of two or more drugs in smaller than usual course doses. This is of no small importance when using antibiotics that cause nephrotoxic (aminoglycosides, polymyxin, gresiofulvin), hepatotoxic (tetracycline, erythromycin) effects, adverse effects on the gastrointestinal tract (tetracyclines, erythromycin, gresiofulvin) and the hematopoietic system (chloramphenicol);

- potentiate the antimicrobial effect;

- prevent or reduce the possibility of the emergence of acquired drug resistance of pathogenic microorganisms.

The proposed method of treatment for bordetella pneumonia in piglets was tested by us with a positive result during 2015-2020. at the collective farm Frunze of the Belgorod district of the Belgorod region on piglets with bordetella pneumonia aged from two weeks to four months. For a given period of time, the proposed method was subjected to treatment of 1500 piglets sick with bordetella pneumonia piglets. Therapeutic efficacy of the composition of farmazin with oleandomycin in a ratio of 1:1 was 90-93%.

The proposed method will find application in disadvantaged farms of the country and will dramatically reduce the incidence of bordetella pneumonia in piglets.

Information sources:

1. "Drug for the treatment and prevention of gastroenteritis and bronchopneumonia in pigs." Certificate of rationalization proposal. Approved GUV GAPK USSR dated June 10, 1986 No. 439-11 / 2015).

2. Materials of the 1st congress of the first congress of veterinary pharmacologists of Russia. Approved 06.21-23.06.2007 - RAAS VNIVIPFiT, - Voronezh, 2007. - P. 311-316).

Claims (1)

A method for treating bordetella pneumonia in piglets, including intramuscular administration of farmazin and oleandiomycin at a dose of 5 mg/kg of body weight of each drug to piglets from 2 weeks to 4 months of age for 7 days.