RU2790682C1 - Method for cultivation of insects of the family sarcophagidae in laboratory conditions - Google Patents

Abstract

FIELD: biotechnology.

SUBSTANCE: invention relates to a method for cultivating flies of the Sarcophagidae family on a nutrient medium consisting of 15–20% of raw meat, 5–7% of gammarus, 23–30% of raw chicken egg, 25–35% of oatmeal (or oatmeal) and the rest of the medium is formed by agar-agar.

EFFECT: achievement of a high level of survival and viability of fly larvae.

1 cl, 1 tbl

Description

The present invention relates to biotechnology and entomology, in particular to a method for cultivating flies of the Sarcophagidae family on a nutrient medium. The method is characterized by the fact that when cultivating flies, a nutrient medium is used, consisting of raw meat 15-20%, gammarus 5-7%, raw chicken egg 23-30%, oatmeal (or oatmeal) 25-35% and agar-agar - the rest.

This issue and the search for the most optimal solutions are widely reflected in the literature.

Works by Khlistovsky E.D. and co-authors [1] are devoted to the creation of nutrient media for growing insects (in particular, the bug of the genus Eurygaster of the family of bugs-tortoises), containing the following ratio of components, wt.%: embryos of grains 40-60; brewer's yeast autolysate 10-30; sucrose 5-15; chlorocholine chloride (60%) 0.5-2.0; methyl ester of p-hydroxybenzoic acid 0.1-0.3; formaldehyde (20%) 0.12-0.36; water is the rest.

And also, a nutrient medium for predatory bugs Arma custos F. and Podisus maculiventris [2] at the following ratio of components, wt.%: trichogram production waste (cryoconserved 20 sieve butterflies) 24.0-26.0; casein according to Hammersten 9.0-11.0; glucose 2.5-3.5; fresh egg white or milk powder 9.0-11.0; ascorbic acid 0.15-0.25; methyl ester of paraoxybenzoic acid 0.15-0.25; formaldehyde 20% 0.23-0.25; cellulose 2.0-2.50; distilled water - the rest.

In the work of Tsoi M.V. [3] much attention is paid to manure as the main component in the cultivation scheme of Hermetia illucens, but unfortunately the author does not provide a detailed recipe for the components on which insects were cultured.

In the patent and scientific and technical literature, the applicants did not find information on the use of a nutrient medium from raw meat, gammarus, raw chicken eggs, oatmeal (or oatmeal) and agar-agar for the purpose of cultivating insects of the Sarcophagidae family, which suggests the presence of an inventive step of the proposal and novelty.

The objective (technical result) of the invention is to increase the survival and viability of insect larvae of the Sarcophagidae family.

The method involves preparing a nutrient medium from raw meat 15-20%, gammarus 5-7%, raw chicken egg 23-30%, oatmeal (or oatmeal) 25-35% and agar-agar the rest. The components must be boiled together until the particles of the medium are completely dissolved. Sterilize at 121°C for 15 min. Pour, following the rules of asepsis, into Petri dishes. Then cool at room temperature for 20 minutes. To achieve the effect, it is necessary to place insects in this nutrient medium starting from the egg stage (or larvae of stages 1, 2) and keep until the pupa stage.

These media provide rapid development, good survival, high viability of larvae due to enrichment with mineral, vitamin and amino acid components, normalization of metabolic processes and nonspecific resistance. The medium is economical to obtain, use and store.

Proper physiological development and high survival is achieved due to the chemical composition of the nutrient medium, which is a diverse list of biologically active substances. Most of which have a great influence on the metabolic and physico-chemical processes occurring in the body of insects.

This nutrient medium contains fats, proteins, carbohydrates, water, vitamins (A, D, D3, E, K, B1, B2, B3, B4, B12, etc.), calcium, iron, magnesium, phosphorus, potassium, sodium, circus, selenium, etc. As well as a large number of amino acids, which ensures the active development of the larvae into adults.

TESTING THE EFFICIENCY OF NUTRIENT MEDIA

Approbation of the effectiveness of a nutrient medium consisting of raw meat 15-20%, gammarus 5-7%, raw chicken egg 23-30%, oatmeal (or oatmeal) 25-35% and agar-agar, the rest was carried out on insects from the Diptera order, family Sarcophagidae.

The experiment was carried out in a room with an air temperature of 26-27°C and an air humidity of 80%. Adults (grey blowflies) were kept in the insectarium, feeding was carried out every 2-3 days by placing pieces of fruit, meat and water in the insectarium. Egg laying was carried out in a laboratory dish - a glass glass with a nominal capacity of 300 ml, a height of 119 ± 4 mm, a width of 67 ± 2 mm, filled with a mixture of raw eggs and oatmeal (or oatmeal) in a ratio of 1:3. After 36-37 hours, we remove the eggs (or larvae of 1.2 stages) and place them in a Petri dish with the nutrient medium described above. Every day we make additional feeding with 10% sugar syrup. After 4 days, the count of individuals that reached the stage of prepupa and larva of the 3rd stage was made. After 11 days, the number of pupae was counted. After 16-17 days, adults were counted - adults (presented in Table 1). At the same time, the indicators were compared with the control experiment, in which there was no additional food. At all stages, the larvae were kept in a Petri dish filled with wheat flour.

Table 1 - Accounting for the survival of the gray blowfly (Sarcophagidae)

Index Control group Experimental group (with the introduction of the studied nutrient medium) Survival after 4 days, % 96 98 Survival after 11 days, % 93 96 Survival after 16-17 days,% 76 90

In the course of the study, we found that the presence of a nutrient medium during the development of insects of the Sarcophagidae family increases the level of survival after 4 days (reaching the stage of prepupa and larva stage 3) by 2%, after 11 days (reaching the stage of pupa) by 3%, after 16 -17 days (reaching the adult stage) by 14%.

Thus, the conducted testing allows us to conclude that this environment ensures the correct development, good survival and viability of the larvae of the Sarcophagidae family.

Literature sources

Pat. SU 551810 A1 Nutrient medium for growing insects Khlistovsky E.D. Shirinyan Zh.A. Alfimov V.A. Published 08/11/1975.

Pat. SU 967440 A1 Nutrient medium for cultivation of predatory insects Khlistovsky E.D. Shirinyan Zh.A. Published 10/23/1982.

Tsoi M.V. Cultivation of the black soldier fly Hermetia illucens (Linnaeus, 1758) (Diptera: Stratiomyidae)/M.V. Tsoi // Scientific and agronomic journal, 2019. - P. 46-48.

Claims (1)

A method for cultivating insects of the Sarcophagidae family in laboratory conditions, characterized in that when cultivating from the egg stage to the pupal stage, a nutrient medium is used, consisting of raw meat 15-20%, gammarus 5-7%, raw chicken egg 23-30%, oatmeal or oatmeal 25-35% and agar-agar the rest.