RU2790467C2 - Portable mobile laboratory based on pneumoframe module - Google Patents

Abstract

FIELD: portable laboratories.

SUBSTANCE: invention relates to portable laboratories and is intended for autonomous laboratory testing of samples of biological material and environmental objects for the presence of markers of pathogens of especially dangerous, other dangerous and natural focal infectious diseases using express and accelerated diagnostic methods in the event of outbreaks of these infectious diseases, as well as in case of implementation of epizootological monitoring of natural foci of bacterial and viral infectious diseases in the field. The effect is achieved by the fact that a portable mobile laboratory based on an inflatable frame module includes an inflatable frame laboratory module with a ridge-type roof and bottom trim, containing a hermetically sealed interior with a biological safety level of BSL-3, with a forced supply and exhaust ventilation system with automatic maintenance of the pressure difference between laboratory compartments, equipped with highly efficient air purification filters, with a single disinfection system, with an additional recirculator for disinfecting the working area, with a visual temperature control system in the room, divided by partitions with a hermetically sealed door into a laboratory compartment, equipped with a transfer lock chamber, a class III microbiological safety box , PCR box, equipment and devices necessary to ensure the preparation of biological material and samples of environmental objects for analysis, DNA and RNA extraction, Odami PCR, MFA, ELISA, and a vestibule for putting on and taking off personal protective equipment; and a technical module for accommodating auxiliary equipment.

EFFECT: increasing the level of biological safety for personnel and reducing environmental contamination.

2 cl, 3 dwg

Description

A portable mobile laboratory based on a pneumatic frame module (PML PM) belongs to portable laboratories and is designed for autonomous laboratory testing of samples of biological material and environmental objects for the presence of markers of pathogens of especially dangerous, other dangerous and natural focal infectious diseases using express and accelerated diagnostic methods for occurrence of outbreaks of these infectious diseases, as well as in the implementation of epizootological monitoring of natural foci of bacterial and viral infectious diseases in the field.

An increase in the efficiency of response to emergencies of a sanitary and epidemiological nature is facilitated by the use of mobile laboratories that can be deployed on the territory of separate, remote, including hard-to-reach areas not covered by a stationary laboratory network, as well as on the territory of natural foci of infections, regardless of their landscape and geographical and climatic characteristics, and to carry out in the proper scope in the field a comprehensive laboratory analysis of samples of biological material and environmental objects using modern methods of express and accelerated diagnostics in compliance with biological safety requirements.

Special pneumoframe modules have small overall dimensions when folded. Inflatable tents can be set up in a short time and used for a long time. Products based on pneumoframework are widely used in various fields as prefabricated premises: to accommodate military units, the civilian population and repair equipment in the field; during the elimination of natural disasters, accidents, man-made disasters; to organize research expeditions and mobile hospitals; as mobile medical posts and temporary headquarters; to create tent camps; as tents for decontamination and disinfection, mobile baths, etc.

The analysis of the prior art carried out by the Applicant, including a search on patents and scientific and technical sources of information, did not reveal information about the use of a product based on a pneumoframe as a portable mobile laboratory for autonomous field diagnostic studies to identify pathogens of infectious diseases of a bacterial and viral nature in biological (clinical) material and environmental objects.

The objective of the invention is to develop an autonomous, easily transportable by any mode of transport, prefabricated on the ground and does not require special conditions when installing a portable mobile laboratory based on an inflatable frame module with wide functionality, which meets the relevant requirements of regulatory documents required when working with PBA I-IV pathogenicity groups and allows to carry out a complex of diagnostic studies (express and accelerated diagnostics by MFA, PCR, ELISA methods) of samples of biological material and environmental objects for the presence of pathogens of especially dangerous and other dangerous and natural focal infectious diseases.

The technical result consists in the implementation of the specified purpose, as well as in increasing the level of biological safety for personnel and reducing environmental contamination. An additional technical result consists in prompt delivery of the laboratory to its destination and commissioning.

The technical solution is achieved by the fact that a portable mobile laboratory based on an inflatable frame module (PML PM) includes an inflatable frame laboratory module with a ridge-type roof and bottom trim, containing a hermetically sealed interior with a biological safety level of BSL-3, with a forced supply and exhaust ventilation system with automatic maintenance of the pressure difference between the laboratory compartments, equipped with highly efficient air purification filters, with a single disinfection system, with an additional recirculator for disinfecting the working area, with a visual temperature control system in the room, divided by partitions with a hermetically sealed door, into a laboratory compartment equipped with a transfer lock chamber , class III microbiological safety box, PCR box, equipment and devices necessary for carrying out and ensuring the preparation of biological material and samples of environmental objects for analysis, DNA and RNA extraction, equipment novation to perform research by PCR, MFA, ELISA; and a vestibule for putting on and taking off personal protective equipment.

In addition, the pneumatic frame laboratory module is equipped with handles for moving to a new place in the assembled state.

In addition, a portable mobile laboratory based on an inflatable frame module includes a technical module for accommodating auxiliary equipment.

The essence of the claimed utility model is illustrated in Fig. 1-3. The claimed laboratory includes a technical module 1 and a pneumoframe laboratory module 2, corresponding to the safety rules for working with microorganisms of I-IV pathogenicity groups. The pneumoframe laboratory module 2 is an inflatable structure with a ridge-type roof. The supporting structure of the pneumatic frame laboratory module is an inflatable sealed PVC frame 3 filled with air using an electric pump. The pneumoframe has a built-in inflation valve and a safety valve (not shown in the figure) that automatically relieves excess pressure. The operating time without pumping is about 3 days, pumping is carried out automatically when the compressor is connected to the mains. The pneumoframe is placed inside the outer tent 4 and aligned with it, which reduces the assembly time during installation. The inner awning 5 is attached to the pneumocarcass with the help of slings with fastex attachment and has the ability to adjust the tension. The air space formed between the inner and outer awnings is a heat insulator and ensures that, with the ventilation system turned on, maintaining an optimal microclimate inside the laboratory room at any ambient temperature readings. The materials used in the production of the pneumoframe laboratory module are resistant to detergents and disinfectants.

The interior of the laboratory module 2 is divided into a laboratory compartment 6 for research; vestibule 7 for putting on and taking off personal protective equipment.

The main entrance is through a vestibule 7, representing the interior with closing inflatable doors 8. Inflatable doors 8 are equipped with separate manual pressure relief valves, equipped with handles and have the ability to lock in the closed position. For greater sealing, doorways are equipped with a safety curtain made of PVC fabric, equipped with a zipper, opening from two sides (not shown in the figure).

In the laboratory compartment 6 there are: folding laboratory tables 9, a mobile box for microbiological safety III class 10; mobile PCR box 11; equipment and devices (not specified) necessary to ensure the preparation of biological material and samples of environmental objects for analysis, DNA and RNA extraction; performing research by PCR, MFA, ELISA. In the room of the laboratory compartment there is a transfer gateway 12, which serves to transfer samples of biological material to the laboratory. The transfer gateway is a separate chamber, access to the interior of which is carried out using two sealed zippers.

The pneumoframe laboratory module 2 is equipped with a unified room disinfection system. The system includes more than one bactericidal irradiator (not shown), bactericidal recirculators 13 are additionally installed to disinfect the air of the working area of the laboratory.

Inside the pneumoframe laboratory module 2 there are fasteners (not shown) for mobile storage systems.

All laboratory equipment and laboratory furniture during transportation is packed and placed in shipping containers, cases, bags 18.

Tambour lock 7 is designed for putting on and taking off personal protective equipment, and is equipped with hangers for clothes and a mirror.

The pneumoframe laboratory module 2 is equipped with an automatic modular supply and exhaust ventilation system 14 and consists of a supply unit 14.1 and an exhaust unit 14.2. The housing of each ventilation module is a shipping container. The supply and exhaust ventilation system is connected to the laboratory module 2 by means of sleeves 15 and connected to the control system with a specialized cable (not specified).

The supply and exhaust ventilation system 14 provides the necessary sanitary and hygienic and microclimatic conditions (including the temperature in the laboratory room 23±2°C in automatic mode); disinfection of the air removed from the working room by equipping the exhaust ventilation system with highly efficient air purification filters of at least H14 class; preliminary two-stage cleaning of the air supplied to the supply system on filters of class not less than G4, F9, cleaning of the air supplied to the working room on a filter of a class of at least NI; protection against ingress of PBA into the environment through the exhaust ventilation system in the event of an emergency with a filter of at least HI4 class; creating and maintaining a negative pressure (vacuum) relative to the environment in the working room, which guarantees the direction of the air flow to a highly efficient air purification filter.

The set temperature in the laboratory room is maintained during the warm period by cooling the air on the freon air cooler of the supply system. In the cold season, the air is heated by built-in heating elements.

The supply and exhaust ventilation system automatically maintains the set air temperature in the laboratory compartment, all filters of the system are equipped with clogging sensors that turn on indicator lamps when their clogging limit values are reached.

Management and synchronization of the operation of all elements of the ventilation and air conditioning system is carried out automatically by the control and monitoring module located on the power supply control panel 17.

Power supply PML is provided from external stationary networks or from an autonomous source of electricity - electric generator 16. The control panel of the power supply system 17 is located between the layers of pneumoframe module with access from the outside.

Technical module 1 is designed to accommodate auxiliary equipment and organize a warehouse, and is a prefabricated tent. The technical module houses 16 power generators that provide power to the PML and are transported inside separate cases; 18 shipping containers that are designed to transport laboratory equipment and folding furniture.

For transportation, the modules and all equipment of the claimed utility model can be packed in separate shipping cases and bags, which ensures the main principle of PML PM - mobility.

Preparation for the work of the claimed laboratory is carried out as follows.

The technical module is being assembled. The pneumatic frame laboratory module is inflated using an electric pump. The pneumoframe laboratory module is installed in a designated area of the terrain, fixed from arbitrary movement with the help of stakes and braces. Install the prefabricated floor and start arranging furniture, equipment and connecting life support systems: ground the module; the input points for the supply of electricity are powered from a stationary network or from an autonomous power source; include lighting systems for laboratory premises, supply and exhaust ventilation. After the parameters of the supply and exhaust ventilation system enter the operating mode (creation of the necessary pressure and ambient temperature in the laboratory), the stationary and removable equipment is connected, and its readiness for operation is checked. Laboratory supplies and reagents corresponding to the workplaces are installed on the desktops. For work, the biological material, packed in accordance with the requirements of sanitary rules, is transferred through the transfer gateway to the laboratory compartment, where it is registered, DNA and RNA are isolated, MFA, ELISA, PCR are performed (identification of pathogen markers - DNA, antigens and antibodies to them). The results obtained are stored on electronic media. Waste and disinfected material is packed in a container for further disposal. Before starting work, the operator enters the airlock, puts on protective clothing and goes to the laboratory. After completion of work, the operator in the airlock carries out chemical disinfection of protective clothing (PPE), its removal and packaging in plastic bags for thermal disinfection.

The design features, technical characteristics and equipment of the PML PM provide constant readiness and the necessary conditions for the comprehensive diagnosis of especially dangerous infections in the field using express and accelerated diagnostic methods, while the personnel are provided with optimal conditions for performing research. The claimed utility model is ergonomic, does not require a large number of maintenance and technical personnel, and can also be transported by various modes of transport. The use of a mobile microbiological safety box III class as part of a portable mobile laboratory based on a pneumoframe module made it possible to ensure not only a high level of biological safety, but also the mobility of the module due to the development of a folding box system with design features and technical characteristics that provide the necessary conditions for working with PBA I- IV group of pathogenicity.

The infrastructure requirements for deploying a mobile lab are minimal. In addition to the possibility of its deployment on the ground, there is the possibility of its use in adaptable premises. Deployment time by 4 people is up to 1 hour.

Reliability and transportability are the key characteristics of a mobile portable laboratory based on a prefabricated pneumoframe module. All laboratory and engineering equipment is packed in cases on wheels protected from water and dust. The equipment is transported in 27 packing places with a total weight of 1127 kg. The mass of packing places allows their transportation as luggage on international airlines.

PML PM can be used for the purpose of epizootological survey of the territory, as well as as a mobile post.

The applicant has manufactured a prototype of the claimed laboratory, which has passed tests, confirming its compliance with the requirements of regulatory documents on the safety of working with PBA of I-IV pathogenicity groups.

Claims (2)

1. A portable mobile laboratory based on an inflatable frame module, including an inflatable frame laboratory module with a ridge-type roof and bottom trim, containing a hermetically sealed cabin with a BSL-3 biological safety level, with a forced supply and exhaust ventilation system with automatic maintenance of the pressure difference between the laboratory compartments, equipped with highly efficient air purification filters, with a single disinfection system, with an additional recirculator for disinfecting the working area, with a visual temperature control system in the room, divided by partitions with a hermetically sealed door to the laboratory compartment, equipped with a transfer lock chamber, a class III microbiological safety box, PCR- box, equipment and instruments necessary to ensure the preparation of biological material and samples of environmental objects for analysis, DNA and RNA extraction, research using PCR, MFA, ELISA, and a tambour gateway for putting on and taking off personal protective equipment; and a technical module for accommodating auxiliary equipment. 2. The portable mobile laboratory according to claim 1, characterized in that the pneumatic frame laboratory module is equipped with handles for moving to a new location in the assembled state.

Images