RU2719621C1 - Fodder additive for cattle - Google Patents

Abstract

FIELD: chemistry.

SUBSTANCE: invention relates to organic chemistry, specifically to a fodder additive for cattle, which includes a chemical composition in the following dosages: 4-(3-hydroxy-1-propenyl)-2-methoxy-phenol – 2.1 mg; 3,4,5-trimethylhydroxyphenol – 0.84 mg; 4-propyl-1,3-benzenediol – 0.65 mg; 4-hydroxy-3-methoxybenzaldehyde – 0.25 mg; 7-hydroxy-6-methoxy-2H-1-benzopyran-2-one – 0.22 mg; 2H-1-benzopyranone-2 – 0.14 mg per kg of the dry substance of the ration.

EFFECT: feed additive is obtained, which increases digestion nutrient content of ration and intensity of growth.

1 cl, 3 tbl

Description

The invention relates to the field of agriculture for use in feeding cattle.

Modern animal husbandry is impossible without feed additives that provide animals with the necessary micronutrients, allowing to solve veterinary and many other problems.

The productivity of ruminants is largely determined by the work of microflora of the pancreas, its abundance and the ratio of individual species. Bacterial populations of ruminant cattle rumen also coordinate community behavior through the cell-cell signaling process mediated by diffusing signal molecules [1, 2].

This circumstance determines the interest in studying the possible mechanisms of QS suppression [3]. At the same time, the expected advantages of the new solutions are minimization of the impact on the natural microflora of cattle, and an increase in productivity.

Previously, scientists [4] found that substances (10%) exhibiting the first type of anti-QS activity in plant extracts [5] also reported a positive effect on animal feeding and growth [6].

It is known that the use of 7-hydroxy-6-methoxy-2H-1-benzopyran-2-one, 2H-1-benzopyranon-2 in in vitro experiments leads to an anti-quorum effect and the formation of biofilms in bacteria [7], as well as the use of derivatives 7 -hydroxy-6-methoxy-2H-1-benzopyran-2-one as an immunostimulating and antiviral agent based on (2 - ((coumarin-7-yl) oxy) ethyl) 3-hydroxy-20 (29) -lupen-28 -oata [8].

The introduction of 4-hydroxy-3-methoxybenzaldehyde into the diet of the aromatic composition contributes to an increase in appetite, resulting in an increase in daily feed intake, thereby increasing productivity [9].

In the experiment, when the composition of chemical compounds similar to ours when growing broiler chickens was included in the broiler’s diet, the experiment revealed that the experimental additive not only affects the suppression of zoopathogenic bacteria, but also increases the live weight gain by 20.3% [10] .

In the Orenburg region, deciduous tree species are represented by broad-leaved species, for example, such as: pedunculate oak, hearty linden, maple, etc. Particular attention is paid to the use of oak bark extract in animal husbandry, since it has an anti-quorum effect [11]; when using the extract, the number of bacteria responsible for fiber digestibility increases [12], thereby contributing to an increase in cattle productivity. When forming the composition of substances, we relied on the identified components in the extract of oak bark and used chemically pure analogues in our studies, namely 4- (3-hydroxy-1-propenyl) -2-methoxy-phenol; 3,4,5-trimethylhydrosciphenol; 4-propyl-1,3-benzenediol; 4-hydroxy-3-methoxybenzaldehyde; 7-hydroxy-6-methoxy-2H-1-benzopyran-2-one; 2H-1-benzopyranon-2. The composition of the extract was determined by high performance liquid chromatography on a gas chromatograph with a mass-selective detector GQCMS 2010 Plus (Shimadzu, Japan) on an HP-5MS column [13].

Analyzing the above, it can be said that 4- (3-hydroxy-1-propenyl) -2-methoxy-phenol; 3,4,5-trimethylhydrosciphenol; 4-propyl-1,3-benzenediol; 4-hydroxy-3-methoxybenzaldehyde; 7-hydroxy-6-methoxy-2H-1-benzopyran-2-one; 2H-1-benzopyranon-2, substances manifest themselves as inhibitors of quorum sensing in bacteria, increase appetite and increase live weight gain of farm animals and poultry, therefore, the use of these components in feeding cattle is promising.

The studies were conducted on the basis of the department for feeding farm animals and feed technology. professors S.G. Leushin Federal State Scientific Institution “Federal Scientific Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences” (Federal Scientific Center of BST RAS).

The following databases were used for laboratory research: Testing Center and Center for Collective Use of the Federal Scientific Center of BST RAS. Scientific and economic experiments were carried out at the production site “Pokrovsky Agricultural College-Branch” of FSBEI HE “Orenburg State Agrarian University”.

In experimental studies, as a feed additive (mg per 1 kg of dry matter of the diet) used a composition of substances manufactured by Acros Organics (USA) [14], the composition of which was as follows:

4- (3-hydroxy-1-propenyl) -2-methoxy-phenol - 2.1;

3,4,5-trimethylhydroxyphenol 0.84;

4-propyl-1,3-benzenediol - 0.65;

4-hydroxy-3-methoxybenzaldehyde 0.25;

7-hydroxy-6-methoxy-2H-1-benzopyran-2-one 0.22;

2H-1-benzopyranon-2 - 0.14.

Preparation of the composition: exact weights of substances were dispersed in an isotonic solution by ultrasonic treatment for 10 minutes at a frequency of 35 kHz (f-35 kHz, N-300 W, A-10 μA) in 1 ml of distilled water.

To study the effect of the composition of substances on the digestibility ratios of nutrients and the dynamics of live weight, 2 groups of calves (n = 3) were formed at the age of 13 months, group I received the main diet, II main diet + composition of substances: 4- (3-hydroxy-1 -propenyl) -2-methoxy-phenol - 2.1 mg; 3,4,5-trimethylhydroskifenol - 0.84 mg; 4-propyl-1,3-benzenediol - 0.65 mg; 4-hydroxy-3-methoxybenzaldehyde - 0.25 mg; 7-hydroxy-6-methoxy-2H-1-benzopyran-2-one 0.22 mg; 2H-1-benzopyranon-2 - 0.14 mg per kg of dry matter of the diet, the preparatory period is 10 days, then they were transferred to the conditions of the main accounting period (8 days). Content at the time of the study - tethered - individual feeding [15]. To study the digestibility of nutrients, a balance experiment was carried out, during which the consumed feed and their residues were taken into account, average stool samples (10%) of the total amount per day were collected, studies were conducted using zootechnical analysis techniques [16].

The growth and development of experimental gobies was studied on the basis of the data of monthly weighing, carried out in the morning, before feeding. According to the results of weighing, the dynamics of live weight and growth by groups were calculated.

The results were processed by the Microsoft Office software package using the Excel program (Microsoft, USA) and the Statistica 10.0 program (Stat Soft Inc., USA). Reliable considered the results from P≤0,05.

In the course of research it was found that the introduction of chemically pure compounds into the diet of the composition of the substance affects the degree of digestion of feed nutrients and the dynamics of livestock in cattle.

An analysis of the data showed that bulls of group II exceeded peers from group I in terms of digestibility of dry matter by 6.07% (P≤0.001), organic by 7.11% (P≤0.001), raw fiber by 8.70% (P≤0.001), BeV by 5.66% (P≤0.001) (Table 1).

Based on this, the use of the proposed composition of substances in the feeding of young cattle can increase the digestibility of nutrients in diets.

During the planning and preparation of the studies, we selected animals of the red steppe breed with a live weight of 290-340 kg. As follows from the results obtained, the use of a composition of substances with anti-quorum activity was accompanied by an increase in the growth rate of young animals and a significant increase in live weight, starting from the second month of the experiment (Table 2).

In particular, by the end of the second month of the main accounting period, the average live weight in the II experimental group reached 373.5 kg, which is 11.1 kg (P≤0.05) exceeded the value of the same indicator in the I group.

By the end of the experiment, at 16 months of age, the largest live weight of animals of group II was 403.2 kg, which was 14.1 kg (P≤0.01) higher than the level of group I.

Assessment of the dynamics of the monthly increase in live weight confirmed the results. Judging by the dynamics of growth in the first month of the experiment, the increase in live weight in the II experimental group amounted to 29.5 kg / goal and significantly exceeded the indicator of the I group by 18.1% (P≤0.01) (Table 3).

During the entire experiment, an increase in live weight was noted by us in group II - 88.78 kg / head or 19.8% (P≤0.001) more than the level of group I.

Thus, the introduction of 4- (3-hydroxy-1-propenyl) -2-methoxy-phenol into the diet; 3,4,5-trimethylhydrosciphenol; 4-propyl-1,3-benzenediol; 4-hydroxy-3-methoxybenzaldehyde; 7-hydroxy-6-methoxy-2H-1-benzopyran-2-one; 2H-1-benzopyranone-2, is accompanied by an increase in the digestibility of nutrients in the diet and an increase in the gain in live weight, which allows us to recommend them as a feed additive for cattle.

Note: * - P≤0.05; ** - P≤0.01; *** - P≤0.001

Note: * - P≤0.05; ** - P≤0.01

Note: * - P≤0.05; ** - P≤0.01; *** - P≤0.001

Literature

1. Schauder, S., Bassler, B.L. The languages of bacteria Genes Dev // Department of Molecular Biology, Princeton University, Princeton. - 2001. - No. 15. - P. 1468-1480.

2. Rutherford, S.T., Bassler, B.L. Bacterial quorum sensing: its role in virulence and possibilities for its control // Cold Spring Harb Perspect Med. - 2012. - T. 11. - No. 2. - P. 235-242.

3. Frederix, M., Downie, J.A. Quorum sensing: regulating the regulators // Advances in Microbial Physiology. - 2011. - T. 195. - No. 16. - P. 3583-3589

4. Deryabin, D.G., Tolmacheva, A.A. Antibacterial and Anti-Quorum Sensing Molecular Composition Derived from Quercus cortex (Oak bark) Extract // Molecules. - 2015 .-- 20 (9). - P. 17093-17108. doi: 10.3390 / molecules200917093

5. Maznev N. Encyclopedia of Medicinal Plants. Moscow: Publishing House of Martin; 2004. pp. 32-33.

6. Redondo L.M., Chacana P.A., Dominguez J.E., Fernandez M.M.E. Perspectives in the use of tannins as alternative to antimicrobial growth promoter factors in poultry. Front Microbiol. 2014; No. 5. P. 118

7. RF patent No. 2616237, Russian Federation A61K, The use of coumarin and its derivatives as inhibitors of “quorum sensing” LuxI / LuxR type in bacteria / Deryabin DG, Tolmacheva AA, Inchagova KS Posted: 04/13/2017

8. RF patent No. 219.017.484 An immunomodulating and antiviral agent based on (2 - ((coumarin-7-yl) oxy) ethyl) 3-hydroxy-20 (29) -lupen-28-oata / Nguyen Thi Hai Yen, Checkerda E.V., Karavaeva A.V., Ivkin D.Yu., Abyshev R.A., Shtro A.A., Nguyen K.B., Abyshev A.Z. Posted on 05/02/2019

9. RF patent No. 2485801, Russian Federation A23K, Flavored composition and method for increasing the appetite of piglets with its help / Crespo Xavier. Posted: 06/27/2013

10. RF patent No. 26499812, Russian Federation A61K, Method for the extraction of a composition of chemical compounds from solid plant materials for the suppression of zoopathogenic bacteria / Deryabin DG, Galadzhieva AA, Miroshnikov SA, Duskayev GK, Rogachev B.G., Pavlov L.N. Posted: 04.04.2018. (prototype)

11. Duskaev, G.K., Deryabin, D.G., Karimov, I.F., Kosyan, D.B., Notova, S.V. Assessment of (in vitro) Toxicity of Quorum Sensing Inhibitor Molecules of Quercus cortex. // J. Pharm. Sci. and Res. - 2018 .-- 10 (1) - P. 91-95.

12. Buryakov, N.P., Ph.D., Buryakova, M.A. Influence of oak extract on rumen microorganisms in feeding nitrate diets to cows // Conference 2006. Influence of oak extract on rumen microorganisms in feeding nitrate diets to cows

13. Tolmacheva, A.A., Rogozhin, E.A., Deryabin, D.G. Antibacterial and quorum sensing regulatory activities of some traditional Eastern-European medicinal plants. // ActaPharmaceutica. - 2014. - 64. - P. 173-186

14. Acros Organics, USA. Website https://www.acros.com/

15. The allowance for research in zootechnology: uch.-methodical. allowance / V.I. Levakhin, N.A. Balakirev, A.V. Kharlamov, G.I. Levakhin, G.K. Duskayev, M.A. Kizaev, I.A. Babicheva, S.I. Mironenko. - Moscow-Orenburg: VNIIMS Publishing House, 2016. 227

16. Lukashik N.A., Tashchilin V.A. Zootechnical analysis of feed. Workshop - M .: Kolos, 1965 .-- 225 s.

Claims (2)

A feed additive for cattle, including a composition of chemicals that support a healthy rumen by limiting the growth of bacteria that cause acidosis in ruminants, while increasing the digestibility of nutrients and the rate of growth, including ingredients in the following dosages (mg per kg dry matter diet) : 4- (3-hydroxy-1-propenyl) -2-methoxy-phenol 2.1 3,4,5-trimethylhydrosciphenol 0.84 4-propyl-1,3-benzenediol 0.65 4-hydroxy-3-methoxybenzaldehyde 0.25 7-hydroxy-6-methoxy-2H-1-benzopyran-2-one 0.22 2H-1-benzopyranon-2 0.14.