RU2716058C1 - Functional food product from black sea mussel mytilus galloprovincialis lam. - Google Patents

Abstract

FIELD: food industry.SUBSTANCE: invention relates to food industry and biotechnology and may be used for production of encapsulated food products from marine origin raw materials. Functional food product of Black Sea mussel Mytilus galloprovincialis lam. contains shell of 1.5–2 % aqueous solution of alginate, filler and preserving agent in form of 10 % aqueous solution of sodium benzoate. Ratio between the calcium alginate cladding and filler is 1:5, and the filler used is raw gonads of the Black Sea mussel Mytilus galloprovincialis lam. 3-4 stages of maturity (pre-spawning-spawning stage).EFFECT: invention enables to obtain a finished product with extended storage life, having radioprotective, antineoplastic and detoxification activity.1 cl, 2 ex

Description

The invention relates to food industry and biotechnology and can be used to obtain encapsulated food products from raw materials of marine origin, for example, the Black Sea mussel Mytilus galloprovincialis lam., Ready for use as dietary products, special food products.

Mussels, including Mytilus galloprovincialis Lam., Which are among the most widespread species in the Azov-Black Sea basin and are successfully cultivated, contain a wide range of biologically active substances that have potential therapeutic properties: immune-stimulating, antitumor, antioxidant, which determined the priority areas of use these mollusks as raw materials for a number of valuable medicinal and therapeutic food products and preparations. In the gonads of mussels cultivated in maritime farms of Crimea, substances of different nature were found: biologically active substances with a high content of bioantioxidants and immunomodulators. Clinical trials have shown that mussel gonad extracts have an immunomodulatory effect and can be used in therapeutic and prophylactic nutrition while eliminating the effects of ionizing radiation on the body [1]. In this regard, the introduction of mussel gonad into the diet is appropriate and justified. A serious obstacle to the widespread use of mussel gonads is that raw mussel gonads should not be stored, and special equipment is needed for transportation.

Known Product from protein and carbohydrate-containing raw materials of water origin, which is a hydrolyzate from squid gonads "Squid" (Patent No. 2093040. RF, IPC A23J 1/04 (1995.01), A23L 1/333, 1994) - dietary supplement for food of general strengthening effect. The method of obtaining the product includes hydrolysis of squid gonad at a temperature of 102 ° C to an amine nitrogen content of 1.2-1.5 wt. %., neutralization to pH 5.0-6.0, evaporation to a solids content of 30-36 wt. %, exposure for at least 5 days and separation of sediment. Contains easily digestible amino acids, oligopeptides, polyunsaturated fatty acids, melanoidins, micro and macro elements. It has high radioprotective, hemostimulating and anti-stress activity. The disadvantage of this method is that during the heat treatment part of the polyunsaturated fatty acids, hormones and other useful substances are lost.

A known method of obtaining a substance from the gonad of mussels m. galloprovincialis with antitumor activity (US Pat. No. 2674033, IPC A61K 35/618, 2017, Russian Federation). The method consists in the fact that raw gonads are isolated from the mussel M. galloprovincialis 4-5 stages of maturity on a five-point scale, which are homogenized in a mixture of chloroform: ethanol, centrifuged, the residue washed with water from non-lipid impurities, the chloroform fraction is evaporated in vacuo. Hexane and aqueous alcohol are added to the obtained lipid residue, the mixture is shaken in a separatory funnel, the hexane fraction is separated, and the aqueous-alcohol fraction is extracted with hexane. Hexane fractions are combined and evaporated in vacuo to constant weight, under certain conditions. The disadvantage of the product obtained in accordance with the method is that toxic chemical solvents (hexane, chloroform) were used. In addition, the radioprotective effect is weakly expressed, because there are no salts of alginic acid, which have pronounced detoxification and radioprotective effects.

Known encapsulated protein-containing product and method for its production (US Pat. No. 2514406, IPC A23L 1/30, 2012, RF). The product has an internal content and an alginic shell based on calcium, barium, or a mixture thereof with a ratio of internal content: shell equal to 100: (20 ÷ 50), while the internal content has a pH of 7.5 ... 8.5. A method of obtaining protein-containing products is to prepare the internal contents with a protein component, followed by encapsulation of the internal contents through a solution of sodium alginate of 0.5-3.0%. The invention allows to obtain an encapsulated protein-containing product that is resistant to destruction at low pH and impermeable to pepsinolysis enzymes. The disadvantage is that the method has not been worked out to obtain a food product based on mussel gonads.

The objective of the invention is to obtain a functional food product based on raw mussel gonads with an extended shelf life.

The technical result consists in the fact that the invention allows to expand the range of useful products based on mussel gonads and the possibility of their use, preserves all the properties inherent in a natural product, including directed biological action. The technical result is that due to the increase in shelf life, the product becomes more accessible to consumers.

The claimed technical result is achieved by the fact that in the Functional food product from the Black Sea mussel Mytilus galloprovincialis lam., Having a shell of a 1.5-2% aqueous solution of alginate, a filler and preservative in the form of a 10% aqueous solution of sodium benzoate, with a ratio between the shell from sodium alginate and a 1: 5 filler, the raw gonads of the Black Sea mussel Mytilus galloprovincialis lam are used as filler. 3-4 stages of maturity (pre-spawning-spawning stages).

The difference between the claimed invention and the prototype lies in the fact that raw gonads of the Black Sea mussel Mytilus galloprovincialis lam. Are used as raw materials, ultivatable in marija farms that are placed in a shell of calcium alginate. The novelty is that a raw, untreated natural product of marine origin, the gonad of the Black Sea mussel Mytilus galloprovincialis lam, is placed in a shell of calcium alginate. 3-4 stages of maturity (pre-spawning-spawning stages).

These distinctive features allow us to conclude that there is novelty in the proposed technical solution. Conducted patent research, as well as the study of available scientific publications related to food products using raw mussel gonadas at a certain stage of development, did not find solutions that have signs similar to the claimed invention, which allows us to conclude that the criterion meets the technical level. Obtaining the claimed product on an industrial scale does not require complex and expensive equipment, it is possible to use equipment for the manufacture of, for example, alginic caviar.

On the basis of the Department of Radiation Pathology of the All-Union Center for Radiation Medicine of the Academy of Medical Sciences of the USSR (Kiev) during 1987-1988. clinical trials were conducted to study the effect of the intake of raw mussel gonadas on the condition of patients who had acute radiation sickness and those exposed to ionizing radiation at lower doses ("Report on the implementation of scientific work by the Department of Radiation Pathology of the Institute of Radiology of the All-Union Scientific Center for Radiation Medicine of the USSR Academy of Medical Sciences" dated September 28 .1988 g.). Based on the studies, the use of raw mussel gonadas in the prevention of the long-term effects of exposure to ionizing radiation was recommended.

It is known that the gonads of fresh mussels can be stored for no more than a day, mussels in closed leaves are stored for no more than 5-6 days. At the same time, 4-5 teaspoons of gonads (2 times a day) correspond to 0.5-1.0 kg of mussels in the leaves. Thus, there is a problem in the delivery and storage of fresh mussel gonads. The proposed technology allows to reduce the mass of the delivered product and increase the shelf life (when canning in jars) up to 6 months without loss of biological value of the gonads.

Calcium alginate enhances the resistance of the product to destructive loads during storage and transportation and increases the biological value of the encapsulated product. Calcium alginate is able to bind and excrete salts of heavy metals, carcinogens, radionuclides, and sodium alginate delays the absorption of radioactive strontium in the intestine, preventing the accumulation of this element in the human body. The beneficial effect of sodium alginate in the complex treatment of diseases of the gastrointestinal tract is also known.

Raw mussel gonads are a complex biologically active complex of substances and the concentration of individual biologically active substances in the gonads of marine hydrobionts exceeds their concentration in the tissues of mussels. The content of biologically active substances in the gonads and the rest of the body mass of mussels varies significantly at different periods of the reproductive cycle. In mussels 3-4 stages of maturity (pre-spawning-spawning stage) longer than 40 mm, because 70-80% maximum carotenoid content. Yellow-orange gonads are preferred, as they have the greatest value due to the high content of carotenoids (respectively, 3.0-5.0 mg and 0.2-3.0 mg of carotenoids per 100.0 g of wet weight).

Sodium benzoate is used as a preservative, calcium chloride is used to create a shell of calcium alginate.

A method of obtaining a functional food product is as follows. In mussels 3-4 stages of maturity (pre-spawning-spawning stage) longer than 40 mm, grown on a marine farm, gonads are extracted. Raw gonads are then mixed with a 1.5-2% aqueous solution of sodium alginate, with a ratio of raw mussel gonads to an aqueous solution of sodium alginate 5: 1. The mixture is treated with a 2% solution of calcium chloride, washed with distilled water and a 10% aqueous solution of sodium benzoate is added.

Example 1

100.0 g of raw mussel gonad is mixed with 20.0 g of a 2% aqueous solution of sodium alginate, treated with a 2% solution of calcium chloride (CaCl ₂ ), washed with distilled water and 0.5 g of sodium benzoate is added in the form of 10% solution.

Example 2

150.0 g of raw mussel gonad are mixed with 30.0 g of a 1.5% aqueous solution of sodium alginate, treated with a 2% solution of calcium chloride (CaCl ₂ ), washed twice with distilled water and 0.75 g of sodium benzoate is added in the form of 10 % solution.

Gonads of mussels were obtained in thermostable casings of calcium alginate, suitable for consumption as proposed and for preservation. The product has a taste and consistency of seafood, oblong irregular shape, size 3-4 cm, weight 1-1.5 g, color from dark orange to light orange, a transparent thin shell covering each gonad. The product can be preserved and stored in sealed jars at a temperature of 5-10 ° C for 6 months. Pasteurization of the product is possible.

Indications for use: contact with ionizing radiation (programmers, copy machine operators affected by the Chernobyl accident); oncology is benign and malignant (including during chemotherapy and radiotherapy); immunodeficiency states; viral infections (SARS, influenza, viral hepatitis), fungal and bacterial infections; disorders of the nervous system (VVD, insomnia, fatigue); living in ecologically unfavorable areas.

Recommended doses: for an adult weighing 70 kg, the dose is 4-5 teaspoons of the resulting product 2 times a day (which corresponds to 0.5-1 kg of mussels in the leaves) for 3-5 months. The product is consumed immediately after breakfast and lunch (you can eat with bread, drink with water).

Thus, based on the available data on the chemical composition of mollusk gonads, as well as the experience of their use, we propose a functional food product based on raw gonads cultivated by M. galloprovincialis lam., Which has radioprotective, antitumor, and detoxification activity.

References taken into account

1. Apryshko T.N., Bad M.V. Antitumor drugs from marine organisms. - Sevastopol: Aquavita, 2000 .-- 106 p.

2. Patent No. 2093040. RF Product from protein and carbohydrate-containing raw materials of water origin and the method of its production. IPC A23J 1/04 (1995.01), A23L 1/333 (1995.01). Besedina T.V., Buga S.A., Korolev A.N., Konoplyanikov A.G., Novikova M.V., Rekhina N.I., Chimirov Yu.I. Applicants and patent holders Rekhina N.I., Besedina T.V., Novikova M.V. Application: 94008589/13, 03/14/1994. Posted: 10.20.1997

3. Pat. 2674033 Russian Federation. IPC ⁷ A61K 35/618, A61P 35/00. A method of obtaining a substance from the gonad of mussels M. galloprovincialis with antitumor activity [Text] / Kapranova L.L., Ryabushko V.I., Nekhoroshev M.V., Apryshko G.I .; Applicant and patentee Federal State Budgetary Institution of Science A.O. Institute of Marine Biological Research Kovalevsky RAS ". No. 2017139047; declared 11/09/2017; publ. 12/04/2018 Bull. Number 34. - 5 sec.

4. Pat. 2514406 Russian Federation. IPC ⁷ A23L 1/30. Encapsulated protein-containing product [Text] / Pivovarov P.P., Pivovarov E.P. (UA); applicant and patent holder Pivovarov P.P., Pivovarov E.P. - No. 2012133693; declared 08/06/2012; publ. 04/27/2014 Bull. No. 12.

Claims (1)

A functional food product from the Black Sea mussel Mytilus galloprovincialis lam., Characterized in that in a product containing a shell of a 1.5-2% aqueous solution of alginate, a filler and preservative in the form of a 10% aqueous solution of sodium benzoate, with a ratio between the shell of calcium alginate and a filler of 1: 5, the raw gonads of the Black Sea mussel Mytilus galloprovincialis lam are used as filler. 3-4 stages of maturity (pre-spawning-spawning stages).