RU2712745C1 - Strain of bacteria paenibacillus jamilae vkpm-13067, used as a fungicide and a stimulator of nodule bacteria - Google Patents

Strain of bacteria paenibacillus jamilae vkpm-13067, used as a fungicide and a stimulator of nodule bacteria Download PDF

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RU2712745C1
RU2712745C1 RU2018142214A RU2018142214A RU2712745C1 RU 2712745 C1 RU2712745 C1 RU 2712745C1 RU 2018142214 A RU2018142214 A RU 2018142214A RU 2018142214 A RU2018142214 A RU 2018142214A RU 2712745 C1 RU2712745 C1 RU 2712745C1
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strain
bacteria
vkpm
fungicide
stimulator
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Сарра Абрамовна Бекузарова
Лилия Евгеньевна Картыжева
Борис Георгиевич Цугкиев
Алан Анзорович Абаев
Вера Ивановна Бушуева
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Федеральное государственное бюджетное учреждение науки Федеральный научный центр "Владикавказский научный центр Российской академии наук"
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

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Abstract

FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology. Disclosed is a bacterium strain of Raibucillus jamilae VKPM V-13067 used as a fungicide and a stimulator of nodule bacteria.
EFFECT: strain has high properties to increase nitrogen-fixing capacity of cultivated leguminous crops with simultaneous reduction of their morbidity.
1 cl

Description

Изобретение относится к области биотехнологии и представляет собой штамм для кисломолочных продуктов и может найти применение в сельскохозяйственном производстве при изготовлении силоса, повышения азотфиксации клубеньковых бактерий в почве, обогащение ее биологическим азотом и как фунгицид.The invention relates to the field of biotechnology and is a strain for fermented milk products and can be used in agricultural production for the manufacture of silage, increasing the nitrogen fixation of nodule bacteria in the soil, enriching it with biological nitrogen and as a fungicide.

Известен штамм, который относится к роду Paenibacillus, с помощью которого подавляют фитопатогенную микрофлору используют в качестве удобрений для сельскохозяйственных культур (патент №2626543, опубликован 28.07.2017. МПК C12N 1/20, C05F 1/08).A known strain that belongs to the genus Paenibacillus, by which the phytopathogenic microflora is suppressed, is used as fertilizer for crops (patent No. 2626543, published July 28, 2017. IPC C12N 1/20, C05F 1/08).

Однако продуктивность данного штамма недостаточна, особенно при возделывании бобовых трав с невысокой активностью клубеньковых бактерий и поражаемостью болезнями.However, the productivity of this strain is insufficient, especially when cultivating legumes with low activity of nodule bacteria and disease susceptibility.

Технический результат получение чистой культуры, обладающей высокими свойствами повышать азотфиксирующую способность возделываемых бобовых культур с одновременным снижением их заболеваемости.EFFECT: obtaining a pure culture with high properties to increase the nitrogen-fixing ability of cultivated legumes with a simultaneous decrease in their incidence.

Техническое решение-выделение местного штамма путем селекции идентифицирован штамм Paenibacillus jamilae ВКПМ 13067 из клубеньковых бактерий корневой системы клевера инкарнатного(Trifolium incarnatum L.).The technical solution is the isolation of a local strain by selection, the Paenibacillus jamilae VKPM 13067 strain was identified from nodule bacteria of the root system of incarnate clover (Trifolium incarnatum L.).

Штамм идентифицирован на выщелоченном черноземе экспериментального участка Северо-Кавказского НИИ горного и предгорного сельского хозяйства Владикавказского Научного центра Российской Академии наук.The strain was identified on leached chernozem of the experimental site of the North Caucasian Research Institute of Mining and Piedmont Agriculture of the Vladikavkaz Scientific Center of the Russian Academy of Sciences.

Культура идентифицирована по 16sp РНК в БРЦ ВКПМ ФГБУ гос. НИИгенетики, г. Москва, 1-й.The culture was identified by 16sp RNA in the BRC VKPM FSBI state. Research Institute of Genetics, Moscow, 1st.

Продукт, синтезируемый штаммом, биологический азот. Активность (продуктивность) штамма - обладает азотфиксирующей активностью в условиях светокультуры. Количество фиксируемого биологического азота составляет 245,2 нм С2Н4/1 растения, за 30 мин.. Способ определения активности штамма - ацетиленовый метод.The product synthesized by the strain, biological nitrogen. The activity (productivity) of the strain - has nitrogen-fixing activity in the conditions of light culture. Number latching biological nitrogen is 245.2 nm C 2 H 4/1 plant per 30 min .. The method for determining the activity of the strain - acetylene method.

Для длительного хранения штамма необходима температура 4°С со скошенной,0 (NH4)2SO4 - 0,05; KН2РO4 - 0,025; K2НРO4×3Н2O - 0,025; MgSO4×7H2O - 0,020; pH - агаризованной средой (среда бобовая) горохового отвара - 10-,0; сахароза - 17; агар-агар - 20,0 Нуждается в пересеве на свежую агаризованную среду через три месяца.For long-term storage of the strain, a temperature of 4 ° C with beveled, 0 (NH 4 ) 2 SO 4 - 0.05; KH 2 PO 4 - 0.025; K 2 HPO 4 × 3H 2 O - 0.025; MgSO 4 × 7H 2 O - 0.020; pH - agar medium (legume) pea decoction - 10-, 0; sucrose - 17; agar-agar - 20.0 Needs reseeding on fresh agar medium after three months.

Культивирование штамма осуществляется при температуре 28°С в течение 3-х суток, в жидкой питательной среде - (бобовая среда)%: гороховый отвар - 10,; сахароза -1,0; (NH4)2SO4 -0,05; KH2PO4 - 0,025; KН2РО4×3Н2O - 0,025; MgSO4 × 7Н20 - 0,020; рН - 7,0.The cultivation of the strain is carried out at a temperature of 28 ° C for 3 days, in a liquid nutrient medium - (bean medium)%: pea broth - 10; sucrose -1.0; (NH 4 ) 2 SO 4 -0.05; KH 2 PO 4 - 0.025; KH 2 PO 4 × 3H 2 O - 0.025; MgSO 4 × 7H 2 0 - 0.020; pH is 7.0.

Критерием отнесения микроорганизма к тому или другому виду считается гомология не менее 97%. По этому критерию исследований штамм можно отнести к виду Paenibacillus jamilae. Анализ филогенетического родства, построенный с использованием типовых штаммов близкородственных бактерий, показал, что наиболее близким к исследуемому штамму является Paenibacillus jamilae, получивший 99,7%.The criterion for classifying a microorganism to one or another species is considered to be at least 97% homology. According to this research criterion, the strain can be classified as Paenibacillus jamilae. An analysis of phylogenetic kinship constructed using typical strains of closely related bacteria showed that Paenibacillus jamilae, which received 99.7%, is the closest to the studied strain.

Штамм обладает фунгицидным действием, продуцирует антибиотики, увеличивает активность клубеньковых бактерий на корнях растений из семейства бобовых культур.The strain has a fungicidal effect, produces antibiotics, increases the activity of nodule bacteria on the roots of plants from the legume family.

Claims (1)

Штамм бактерий Раеnibacillus jamilae ВКПМ В-13067, используемый как фунгицид и стимулятор клубеньковых бактерий.The bacterial strain Ranibacillus jamilae VKPM B-13067, used as a fungicide and stimulator of nodule bacteria.
RU2018142214A 2018-11-29 2018-11-29 Strain of bacteria paenibacillus jamilae vkpm-13067, used as a fungicide and a stimulator of nodule bacteria RU2712745C1 (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2539738C1 (en) * 2013-08-20 2015-01-27 Закрытое акционерное общество научно-производственное предприятие "Биомедхим" (ЗАО НПП "Биомедхим") STRAIN OF BACTERIA Paenibacillus sp. FOR OBTAINING BIOLOGICAL PRODUCT AGAINST DISEASES OF WHEAT CAUSED BY PHYTOPATHOGENIC FUNGI
WO2016020371A1 (en) * 2014-08-04 2016-02-11 Basf Se Antifungal paenibacillus strains, fusaricidin-type compounds, and their use
RU2626543C2 (en) * 2015-11-09 2017-07-28 Общество с ограниченной ответственностью "Промышленные Инновации" Paenibacillus mucilaginosus bacteria strain, method for plants growth stimulation and protection against diseases and application of paenibacillus mucilaginosus bacteria strain as fertiliser and biological control agent (antipatogenic means) in prevention and/or treatment of plant disease
KR101771960B1 (en) * 2015-09-17 2017-08-28 대한민국 Paenibacillus jamilae BRC15-1 strain producing cellulase and use thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2539738C1 (en) * 2013-08-20 2015-01-27 Закрытое акционерное общество научно-производственное предприятие "Биомедхим" (ЗАО НПП "Биомедхим") STRAIN OF BACTERIA Paenibacillus sp. FOR OBTAINING BIOLOGICAL PRODUCT AGAINST DISEASES OF WHEAT CAUSED BY PHYTOPATHOGENIC FUNGI
WO2016020371A1 (en) * 2014-08-04 2016-02-11 Basf Se Antifungal paenibacillus strains, fusaricidin-type compounds, and their use
KR101771960B1 (en) * 2015-09-17 2017-08-28 대한민국 Paenibacillus jamilae BRC15-1 strain producing cellulase and use thereof
RU2626543C2 (en) * 2015-11-09 2017-07-28 Общество с ограниченной ответственностью "Промышленные Инновации" Paenibacillus mucilaginosus bacteria strain, method for plants growth stimulation and protection against diseases and application of paenibacillus mucilaginosus bacteria strain as fertiliser and biological control agent (antipatogenic means) in prevention and/or treatment of plant disease

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