RU2679603C1 - Disinfectant - Google Patents

Abstract

FIELD: medicine.SUBSTANCE: group of inventions relates to the field of medicine, namely to hygiene and sanitation, and is intended for disinfecting surfaces, cleaning and deodorizing the air in rooms. Disinfectant contains hydrocarbon propellant, ethyl alcohol, perfume composition and benzalkonium chloride. Components are used in claimed amounts. Specified disinfectant is used for cleaning, deodorizing and disinfecting air and surfaces from such viruses, as Poliovirus, Adenovirus, Murine Norovirus, as well as from microorganisms such as Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Enterococcus hirae, Aspergillus brasiliensis (ex. A. niger), Candida albicans, Trichophyton mentagrophytes.EFFECT: use of a group of inventions provides effective disinfection of surfaces, cleaning of air and various surfaces, while the tool does not leave splashes and stains on pieces of furniture and plumbing and has a rich floral aroma with a slight bitter nuance of coniferous wood resin.9 cl, 4 ex, 6 tbl

Description

The invention relates to the field of hygiene and sanitation and is intended for disinfection of surfaces, purification and deodorization of air in the premises of the household and the general living environment, as well as for the prevention of colds.

A large number of disinfectants and deodorizing agents are known, which include components such as halogens, alcohols, peroxides, phenols, quaternary ammonium compounds, aldehydes, tertiary amines and acids (Kolosovskaya E.N., Tekhova I.G. Current state of choice disinfectants in medical institutions // Clinical epidemiology: “Terra Medica”, No. 1, 2010).

Air fresheners help get rid of unpleasant, harsh and asphyxiating odors that fill the room or room. In the process of their use, the enclosed space is saturated with the freshness and light aroma of flowers, herbs or citrus fruits.

In addition, there is a category of products that do not just neutralize polluted air and aromatize it. This type of freshener additionally has a disinfecting property. By eliminating unpleasant odors, they destroy harmful bacteria in the room and prevent their further spread.

So from China patent CN 103768629 an air freshener is known, which includes dimethyl ether as a propellant in an amount of 20-25 parts, morpholine 0.1-0.5 parts, benzalkonium chloride 0.2-0.5 parts, ethanol from 50 up to 55 parts, water from 15 to 20 parts, essential oil from 0.15 to 10 parts, tetraethylene glycol from 5 to 10 parts, N-dodecyl di-quaternary ammonium salt from 0.1 to 0.2 parts. As an essential oil in the freshener, peppermint and lemon essential oils are used. Air purification in the room occurs by absorbing harmful air particles with freshener particles, and the aromatic substances that make up the air freshener can hide an unpleasant odor in the room. Benzalkonium chloride in the form of an aqueous solution provides a bactericidal effect. The addition of peppermint oil provides a fresh aroma, and ethanol provides a disinfectant and antibacterial effect.

An antibacterial air freshener is known from Chinese Patent Application CN105999341, comprising the following components: peppermint oil, rose oil, lavender oil, wormwood oil, rosemary oil, ethanol, water. The volume ratio of peppermint oil, rose oil, lavender oil, wormwood oil, rosemary oil is (1-10): (5.5-10.5): (3-9.4): (5-6.8): ( 2-8): (3.2-7.5): (2-15), water - the rest. Ethanol in the composition of the freshener is used in the form of a 95% solution. An air freshener can effectively purify the air of pathogenic bacteria, such as Escherichia coli, Staphylococcus aureus, etc.

From Chinese patent application CN 105685118 an air freshener is known which contains the following components in wt. parts: 20 parts of cinnamon oil, 20 parts of lemon oil, 20 parts of pine essential oil, 20 parts of thyme oil, 10 parts of oregano oil and 20-50 parts of alcohol and 150 parts of water. Air freshener has antibacterial, antifungal and antiviral effects.

The composition of all agents known from the prior art includes water, which is inconvenient to use, since such agents leave splashes and stains on furniture and plumbing, and the aroma of such agents does not have a long-lasting effect.

The closest analogue of the claimed invention is a deodorizing agent known from RF patent No. 2438711, which contains a hydrocarbon propellant, coriander essential oil and ethyl alcohol, denatured ethyl alcohol is used, the agent further comprises a perfume composition, zinc ricinoleate and silver citrate in the following ratio of components , wt.%:

Coriander essential oil 0.001-0.500 Perfumery composition 0,100-3,000 Denatured ethyl alcohol 1.00-30.000 Zinc ricinoleate 0.010-1.000 Silver citrate 0.010-0.500 Hydrocarbon propellant Rest

Denatured ethyl alcohol is used as a solvent and preservative of the active components. As a perfume composition, a mixture of synthetic or natural fragrances is used, which is a key component of air fresheners, which is responsible for the direction of the smell of the final product. As the hydrocarbon propellant, propane, butane, isobutane or a mixture thereof is used. The use of essential coriander oil provides an antiseptic effect. The product has a long time of the presence of a pleasant smell with both high efficiency and duration of antibacterial and antimicrobial effects, in particular, bactericidal properties against gram-negative and gram-positive bacteria and fungicidal properties against yeast-like fungi and mold.

The disadvantage of the closest analogue is the inability to use funds for disinfection and cleaning of surfaces.

The objective of the invention is the creation of a universal disinfectant with a wide range of applications, designed both for quick and effective disinfection of surfaces, and for cleaning and deodorizing air in household premises and the general living environment, which has a pleasant aroma

This problem is solved using a disinfectant, the main components of which are ethyl alcohol, benzalkonium chloride, perfume composition and hydrocarbon propellant.

The ratio of components in the disinfectant in wt.%:

Ethanol 29.1-29.8 Benzalkonium chloride 0.15-0.30 Perfumery composition 0.06-0.60 Hydrocarbon propellant Rest.

Ethyl alcohol in this product is a disinfectant and solvent.

Benzalkonium chloride provides a prolonged disinfecting effect on surfaces and bactericidal activity against staphylococci, streptococci, gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa, etc.), anaerobic bacteria, fungi and molds.

Perfume composition is a component of the disinfectant, responsible for the formation of odor.

Perfume composition contains from 0.5 to 13 wt. % of essential oils such as rosemary, lavender, pine, pink. A common property of these essential oils is their antiseptic and antibacterial effect.

The essential oils present in the perfume composition, when evaporated in the rooms, contribute to deodorization of the air and prevent the development of microorganisms.

Essential oils of plants are largely composed of mono-, sesqui and diterpenes and their derivatives. Monoterpenes are present in almost all essential oils, sesquiterpenes are often found in the chemical composition of essential oils, and diterpenes are present in small quantities. Monoterpenic and sesquiterpenic are valuable in the following properties: they are strong antiseptics, have a bactericidal and fungicidal effect, have antiviral properties (D. Muravyova Pharmacognosy: Textbook. - 3rd ed., Revised. And add. - M .: Medicine, 1991 , - 560 p .; Leonova N.S. Aromatherapy for beginners .-- M .: Fair-Press, 2007, - 118 p.).

Rosemary oil is a colorless or light yellow liquid with a strong woody-grassy odor. Rosemary oil contains up to 60% monoterpene hydrocarbons, 1,8-cineole, camphor, borneol, linalool, as well as borne acetate and caryophylline. The oil is a strong antiseptic, has a bactericidal and fungicidal effect (Leonova N.S. Aromatherapy for beginners. - M .: Fair-Press, 2007, - 118 p .; Voitkevich S.A. Essential oils for perfumery and aromatherapy. - M. , "Food Industry", 1999, - 282 p.).

Essential oils containing 1,8-cineole (eucalyptol), in particular rosemary oil, lavender oil, have antiseptic activity (antibacterial, antiviral) and are excellent means of preventing bacterial and viral infections transmitted by airborne droplets (Leonova N.S. Aromatherapy for beginners. - M .: Fair-Press, 2007, - 118 p .; Voitkevich SA Essential oils for perfumery and aromatherapy. - M., Food Industry, 1999, - 282 p.).

Lavender oil is a light yellow liquid with a strong lavender-camphor odor. The total content of linalyl acetate and linalool is about 60%. Camphor, 1,8-cineole and borneol are present in the oil. Lavender oil has an antifungal effect, has pronounced bactericidal properties.

Pine oil is a colorless or yellowish liquid with a balsamic-turpentine odor. The group chemical composition of the essential oil includes up to 75% of monoterpene hydrocarbons, about 15% of sesquiterpenes, and up to 8% of bornyl acetate and other oxygen-containing terpenoids. The oil has a bactericidal and virucidal effect (Leonova N.S. Aromatherapy for beginners. - M .: Fair-Press, 2007, - 118 s .; Voitkevich S.A. Essential oils for perfumery and aromatherapy. - M., "Food industry ", 1999, - 282 p.).

Rose oil is a thick transparent liquid of yellow or greenish-yellow color with a pronounced smell of roses. The main components of rose oil are geraniol and citronellol. Rose oil has antiseptic properties (Leonova N. S. Aromatherapy for beginners. - M .: Fair-Press, 2007, - 118 p .; Voitkevich S. A. Essential oils for perfumery and aromatherapy. - M., "Food industry" , 1999, - 282 p.).

Essential oils in the perfume composition are in the following amounts in wt.%: Rosemary oil - 0.5%; pine oil - 2.0%; lavender oil - 4.0%; rose oil - 5.0%.

The composition of the perfume composition also includes a mixture of components, such as geraniol, linalool, terpeniol, thymol, menthol, which not only add a peculiar aroma, but also contribute to the manifestation of antiseptic activity against bacterial and viral infections. The content of the mixture of these components is 30 to 40 wt.%.

A mixture of components with floral notes that complement the aroma of the perfumery composition and is present in an amount of 35 - 45 wt.%;

The component necessary when creating perfumes, combining all the ingredients into a single composition in an amount of 10 to 20 wt.% (The composition of the component is not disclosed, because it is the know-how of the applicant).

In the complex, these components of the disinfectant allow you to clean and deodorize the air, whereby a therapeutic and preventive effect is achieved.

An important advantage of the claimed funds is also the fact that during the cleaning and disinfection of air and surfaces using disinfectants, the disinfecting components that make up such agents leave an unpleasant and pungent odor. The essential oils that make up the product can not only enhance the bactericidal, virucidal and fungicidal activity of the disinfectant, but also hide the unpleasant and pungent odor of the disinfectant.

The combination of essential oils with floral fragrances allows you to get a rich floral aroma with a light bitter shade of resin of coniferous wood.

The disinfectant according to the invention has a bactericidal, fungicidal and virulent effect, antimicrobial activity against gram-negative and gram-positive microorganisms, it is effective against enteroviruses (including adeno- and polioviruses), influenza A and B viruses, it is effective against mold and yeast fungi.

The effectiveness of the disinfectant on the surfaces is confirmed by the results of laboratory tests conducted in accordance with the requirements of standardized EU procedures and is illustrated by examples.

Research was conducted on test systems using test objects.

Example 1. Verification of bactericidal activity.

The study of bactericidal activity was carried out in accordance with the standard EVS-EN 1276: 2009.

For the study, a disinfectant was used containing as active components: ethyl alcohol 29.79 wt.%, Benzalkonium chloride 0.15 wt.%, Perfume composition 0.06 wt.% And hydrocarbon propellant - 70 wt.%.

The concentration of the tested disinfectant is 80 wt.%.

For research used bovine serum albumin concentration of 0.3 g / L.

Temperature of the test system: 20 ± 1 ^о С. Incubation temperature: 36.5 ± 0.5 ° С. The contact time of the disinfectant with the infected surface is 15 seconds, 30 seconds, 1 minute.

Bactericidal activity was tested on the following microorganisms: Escherichia coli ATCC 10536, Staphylococcus aureus ATCC 6538, Enterococcus hirae ATCC 10541, Pseudomonas aeruginosa ATCC 15442.

The test results are presented in table 1.

Table 1.

Organisms test contact time Vc1 Vc2 Na lg Na R (log No - log Na) Escherichia coli ATCC 10536 15 sec 0 0 <140 <2.15 > 5.35 30 sec 0 0 <140 <2.15 > 5.35 1 min 0 0 <140 <2.15 > 5.35 Staphylococcus aureus ATCC 6538 15 sec 0 0 <140 <2.15 > 5.28 30 sec 0 0 <140 <2.15 > 5.28 1 min 0 0 <140 <2.15 > 5.28 Pseudomonas aeruginosa ATCC 15442 15 sec 0 0 <140 <2.15 > 5.38 30 sec 0 0 <140 <2.15 > 5.38 1 min 0 0 <140 <2.15 > 5.38 Enterococcus hirae ATCC 10541 15 sec 0 0 <140 <2.15 > 5.47 30 sec 0 0 <140 <2.15 > 5.47 1 min 0 0 <140 <2.15 > 5.47

The values shown in table 1 were calculated by the formulas:

N = s / (n1 + 0.1 n2) x d min

Xsr = (Vc1 + Vc2) / 2

Na = 10 X / n

where, N is the number of microorganisms of the test suspension (CFU / ml); c is the average number of surviving test microorganisms in CFU per 1 ml in plates taken for calculation; n1 = 2, in the case of using 2 cups with a dilution of a multiple of 10 ^-6 ; n2 = 2, in the case of using 2 cups with a dilution multiple of 10 ^-7 ; d min - the degree of least dilution, in the case, the calculation of microorganisms for two cups with different dilutions; d is the degree of least dilution for the case if for calculation only cups of the same dilution are taken into account; R - reduction factor (log R = log Nо - log Na); Vc is the number of surviving colonies; Na is the number of CFU / ml in the test mixture; Hsr. - arithmetic average number of surviving colonies CFU / ml; CFU - colony forming units.

The disinfectant has high bactericidal activity against bacterial strains Escherichia coli ATCC 10536, Staphylococcus aureus ATCC 6538, Enterococcus hirae ATCC 10541, Pseudomonas aeruginosa ATCC 15442, with a contact time of 15 seconds and a study temperature of 20 ° C, because the reduction factor is R> 5 lg.

Example 2. Check fungicidal activity.

The study of fungicidal activity was carried out in accordance with the standard EN 1650: 2008 + A1: 2013.

For the study, a disinfectant was used containing ethyl alcohol 29.14 wt.%, Benzalkonium chloride 0.30 wt.%, Perfume composition 0.60 wt.% And hydrocarbon propellant 69.96 wt.% As active components. The concentration of the tested disinfectant is 80 wt.%.

For research used bovine serum albumin concentration of 0.3 g / L.

Temperature of the test system: 20 ± 1 ^о С. The contact time of the disinfectant with the infected surface is 15 seconds, 30 seconds, 1 minute, 3 minutes, 5 minutes, 7 minutes, 10 minutes

Fungicidal activity was tested on the following microorganisms: Candida albicans ATCC 10231, Trichophyton mentagrophytes ATCC 9533, Aspergillus brasiliensis ATCC 16404.

The test results are presented in table 2.

Table 2.

Organism test Vc1 Vc2 Na x 10 lg Na lg R Contact time Candida albicans TCC 10231 2 one <140 <2.15 > 4.37 15 sec 0 0 <140 <2.15 > 4.37 30 sec 0 0 <140 <2.15 > 4.37 1 min Trichophyton mentagrophytes ATCC 9533 one 3 <140 <2.15 > 4.03 30 sec 0 0 <140 <2.15 > 4.03 1 min 0 0 <140 <2.15 > 4.03 3 min Aspergillus brasiliensis ATCC 16404 > 165 > 165 > 8200 3.91 2.27 30 sec 80 84 > 165 > 165 4100 3.61 2,57 1 min 39 43 45 55 500 2.7 3.48 3 min 8 8 29th 345 315 2,5 3.68 5 minutes four 3 13 10 115 2.06 4.12 7 min one 2 6 6 fifty 1.7 4.48 10 min one one

The value of the indicators shown in table 2 was calculated by the formulas:

N = s / (n1 + 0.1 n2) x 10 ^-5

Na = 10 s / n

No = N / 10

Nvo = c / n

A, B, C = c / n

lg R = lg No– lgNa

where, R - reduction factor (log R = log Nо - log Na); Vc is the number of surviving colonies; N is the number of microorganisms of the test suspension (CFU / ml); ); c is the average number of surviving test microorganisms in CFU per 1 ml in plates taken for calculation; n1 = 2, in the case of using 2 cups with a dilution of a multiple of 10 ^-6 ; n2 = 2, in the case of using 2 cups with a dilution of a multiple of 10 ^-7 ; Nvo is the number of test microorganisms in CFU / ml in a validation suspension when diluted 10 times during the preparation of controls; Na is the number of CFU / ml in the test mixture; A is the number of CFU / ml for monitoring experimental conditions; B is the number of CFU / ml to control the toxicity of the neutralizer; C is the number of colonies to control the neutralization method; CFU - colony forming units.

The disinfectant has a high fungicidal activity in the suspension test at a contact time of 15 seconds. at 20 ºC in relation to the organism test Candida albicans ATCC 10231, and under the same conditions, the product has fungicidal activity at a contact time of 30 seconds in relation to an additional organism test Trichophyton mentagrophytes ATCC 9533 and under the same conditions at a contact time of 7 min. in relation to Aspergillus brasiliensis ATCC 16404, since the reduction factor is R> 4 log.

Example 3. Verification of virucidal activity.

The study of virucidal activity was carried out in accordance with the standard UNE-EN 14476: 2014 + A1: 2015.

For the study, a disinfectant was used containing 29.79 wt.% Ethyl alcohol, 0.15 wt.% Benzalkonium chloride, 0.06 wt.% Perfume composition and 70 wt.% Hydrocarbon propellant as active components. For the study, the agent was used in the form of a solution; sterile distilled water was used as a solvent. The concentration of the tested disinfectant is 80 wt.%, 50 wt.%, 0.1 wt.%.

For research used bovine serum albumin concentration of 0.3 g / L. Virus control. An aqueous formaldehyde solution (formaldehyde) was used as a comparison solution, an aqueous formaldehyde solution containing a test virus (formaldehyde control virus) was used as a control solution.

The temperature of the test system 20 ± 1 ° ^C. Analysis Temperature 37 ± 1 ° C. The contact time of the disinfectant with the infected surface is 5 minutes.

Virucicidal activity was tested on the following viruses: Poliovirus type 1 (ATCC VR-192); Adenovirus type 5 (ATCC VR-5); Murine norovirus (strain S99 Berlin).

The test results are presented in tables 3-5.

Table 3. Virucidal activity against Poliovirus type 1 virus (ATCC VR-192)

Product The concentration of the tested disinfectant, wt.% log10 TCID ₅₀ via: Decrease at 95% confidence interval after 5 minutes 0 min 1 min 5 minutes 30 minutes 60 min The disinfectant according to the invention 80 - - 2.41 - - 4.58 ± 0.49 fifty - - 4.08 - - 2.91 ± 0.53 0.1 - - 5.99 - - 1.00 ± 0.54 Formaldehyde 0.7 - - - 5.41 3.99 NP Virus control NP 6.74 HP 6.99 HP HP NP Formaldehyde control virus 0.7 6.83 HP HP HP 6.99 NP

Table 4. Virucidal activity against Adenovirus type 5 virus (ATCC VR-5)

Product Test disinfectant concentration log10 TCID ₅₀ via: Decrease with a confidence interval of 95% after 5 minutes 0 min 1 min 5 minutes 30 minutes 60 min The disinfectant according to the invention 80 - - 1.41 - - 5.16 ± 0.65 fifty - - 3,50 - - 3.07 ± 0.62 0.1 - - 6.08 - - 0.49 ± 0.68 Formaldehyde 0.7 - - - 2.83 2.24 NP Virus control NP 6.66 HP 6.57 HP HP NP Formaldehyde control virus 0.7 6.66 HP HP HP 6.75 NP

Table 5 Virucidal activity against the virus Murine norovirus (strain S99 Berlin)

Product Test disinfectant concentration log10 TCID ₅₀ via: Decrease with a confidence interval of 95% after 5 minutes 0 min 1 min 5 minutes 30 minutes 60 min The disinfectant according to the invention 80% - - 0.50 - - 5.75 ± 0.26 fifty% - - 1,66 - - 4.59 ± 0.34 0.1 - - 5.07 - - 1.18 ± 0.48 Formaldehyde 0.7 - - - 4.41 2.83 NP Virus control NP 6.07 HP 6.25 HP HP NP Formaldehyde control virus 0.7 5.66 HP HP HP 5.83 NP

where, log10 TCID50 - indicator of the decrease in viral activity: decimal logarithm of the infectious dose of tissue culture (Tissue culture infective dose 50% - infectious dose of tissue culture 50%.)

Virucidal activity exists when the titer of the virus shows a decrease of ≥4 log.

NP - not applicable; HP - not implemented

At an exposure time of 5 minutes, the disinfectant according to the invention exhibits virucidal activity against three essential viruses: Poliovirus type 1, Adenovirus type 5, Murine norovirus, when product activity is evaluated in accordance with UNE-EN 14476: 2014+ A1: 2015.

The effectiveness of a disinfectant for air purification is confirmed by the following example.

Example 4

Studies on air purification were carried out in a closed room (box) with a volume of 30 m ³ during artificial infection of the air with test organisms, for which Staphylococcus aureus was used. The initial level of artificial air infection with test organisms was 106-107 CFU / m ³ , which is typical for a high level of airborne contamination of the premises. Then, to simulate the constant presence of people, the air was additionally infected by spraying a culture of microorganisms at a level of 105 CFU / m ³ .

The study was carried out at room temperature.

The agent according to the invention was sprayed in air for 10 seconds.

Air samples were taken and the state of the air in the box was evaluated at regular intervals by the number of microorganisms in the volume of the air sample.

The data obtained are shown in table 6.

Table 6.

No. p / p Time after infection, min The concentration of bacteria, CFU / m ³ one 0 9.6 * 10 ⁶ 2 fifteen 0 3 thirty 0 four 60 0

From the data obtained it follows that the disinfectant according to the invention has high bactericidal efficacy with an exposure time of less than 15 minutes in air.

The results of the tests show that the disinfectant is a universal tool for both air purification and for cleaning various surfaces, since it provides high bactericidal, virucidal and fungicidal efficacy in its application, both for air purification and for cleaning various surfaces.

The tool is safe and convenient to use because it does not contain water and other components that can leave splashes and stains on furniture and plumbing.

In addition, the combination of floral and coniferous aromas introduced by the disinfectant, essential oils and floral fragrances creates a favorable atmosphere in the premises.

Claims (11)

1. A disinfectant containing hydrocarbon propellant, ethyl alcohol and perfume composition, characterized in that the composition of the tool includes benzalkonium chloride in the following ratio, wt.%: Ethanol 29.8-29.1 Benzalkonium chloride 0.15-0.30 Perfumery composition 0.06-0.60 Hydrocarbon propellant rest, while the perfume composition contains a mixture of essential oils of rosemary, lavender, pine and rose, as well as floral fragrances. 2. The disinfectant according to claim 1, characterized in that the content of the mixture of essential oils in the perfume composition is 0.5-13 wt.%. 3. The disinfectant according to claim 2, characterized in that the essential oils in the perfume composition are in the following amount: rosemary oil - 0.5 wt.%; pine oil - 2.0 wt.%; lavender oil - 4.0 wt.%; rose oil - 5.0 wt.%. 4. The disinfectant according to claim 1, characterized in that the composition of the perfume also includes a mixture of components having activity against bacterial viral infections, such as geranilol, linalool, terpeniol, thymol, menthol, in an amount of 30-40 wt.% , a mixture of components with floral notes in an amount of 35-45 wt.%, as well as a component necessary when creating perfumes and combining all the ingredients in a single composition, in an amount of 10-20 wt.%. 5. The disinfectant according to claim 1, characterized in that propane, butane, isobutane or mixtures thereof are used as the hydrocarbon propellant. 6. The use of a disinfectant according to claim 1 for cleaning, deodorizing and disinfecting air and surfaces. 7. The use according to claim 6 in relation to viruses such as Poliovirus, Adenovirus, Murine Norovirus. 8. The use according to claim 6 in relation to such microorganisms as Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Enterococcus hirae. 9. The use according to claim 6 in relation to such microorganisms as Aspergillus brasiliensis (ex. A. niger), Candida albicans, Trichophyton mentagrophytes.