RU2732763C1 - Disinfectant based on quaternary ammonium compounds and phytoncides - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention refers to medicine, sanitation and disinfectology, namely to disinfection means with pronounced antiseptic action intended for disinfection of air medium, surfaces in rooms, skin, wound surfaces. Disclosed disinfectant composition contains a quaternary ammonium compound (QAC) selected from benzalkonium chloride, 1,2-ethylene-bis-(N-dimethylcarbdecyloxymethyl) ammonium dichloride (ethonium) or a mixture thereof, 2-phenoxyethanol or a mixture thereof with ethylhexyl glycerol, at least one phytoncide source, which is a phyto-extract and/or essential oil of plants selected from cedar, common Scots pine, marigolds, Litsea cubeba, eucalyptus, monads, sage, marigold, oregano, juniper, wormwood, structure-forming agent selected from Aristoflex (copolymer of acrylic acid), carbomer, sodium carboxymethyl cellulose (blanose) or mixtures thereof, and water.

EFFECT: agent exhibits antimicrobial activity against bacteria and viruses, is stable during storage and is safe for application on skin.

1 cl, 1 dwg, 1 tbl, 8 ex

Description

The invention relates to the field of medicine, sanitation and disinfectology, namely to disinfectants with a pronounced antiseptic effect, intended for disinfection of the air, surfaces in rooms, skin, wound surfaces, as well as for the prevention of the spread of infectious diseases of bacterial, viral and fungal etiology.

Decontamination of microorganisms is a complex multifactorial process in which the concentration of the disinfectant (DS), the method of its use and the exposure time play a leading role. The action of DS is differently effective for different types of microorganisms, which is associated with differences in the organization of their surface structures and metabolism. The development of new means for disinfection is an urgent task, since microorganisms are able to adapt very quickly to long-term used disinfectants.

More than 40 types of bacteria are capable of provoking diseases that lead to disability. The most common are Staphylococcus aureus, Salmonella enteritidis, Pseudomonas aeruoginosa, Klebsiella pneumonia, Escherichia coli, Aspergillus niger, Candida albicans, among which multidrug-resistant strains are often found. The mortality rate of patients from bacterial infections, including those from nosocomial infections, remains at a high level. The situation in maternity hospitals is aggravated by the fact that there is a carrier of bacteria among the staff. The limited time for the choice of antibacterial drugs, primarily antibiotics, as well as the resistance of bacteria to the groups of fluoroquinolones, macrolides, synthetic penicillins, glycopeptides, aminoglycosides, fenicols and tetracyclines dictates the need to expand the spectrum of DS. Material from patients often contains multidrug-resistant bacteria that are resistant to three or four, and in some cases, to all of the above groups. In addition, there is a tendency for an increase in the number of allergic reactions to chemical agents among medical personnel and among employees, where hygiene rules are required. Therefore, attempts by domestic scientists to create alternative antibacterial drugs are an urgent task.

The range of agents based on chemical active substances, such as hydrogen peroxide, chlorine-containing, fluorine-containing and ethanol-containing ones is quite wide (Kolosovskaya E.N., Tekhova I.G.). The current state of the choice of disinfectants in medical institutions // Clinical epidemiology: "Terra Medica", No. 1, 2010). However, they can cause various allergic reactions. The technology for obtaining these funds is also unsafe.

Due to their antiseptic effects, products based on quaternary ammonium compounds are widely used in the field of disinfection and hygiene, many of which are protected by patents.

For example, RF patent 2272652, publ. 03/27/2006, discloses a disinfectant comprising alkyldimethylbenzylammonium chloride, glyoxal, glutaraldehyde, lower alcohol, hydrophilic nonionic surfactant, Trilon B, hydrophobic nonionic surfactant, tributyl or tricresyl phosphate, and functional additives. The disinfectant can be used to disinfect sanitary equipment, patient care items, medical supplies, etc.

Known disinfectant containing a quaternary ammonium compound (QAC), triamine, a mixture of polyhexamethylene guanidine chloride and polyhexamethylene biguanidine chloride, n-propyl alcohol, wetting agent Triton X-100, complexing agent EDTA, antifoam agent is sodium chloride. The product is intended for disinfection of the air and surfaces in rooms (RU 2646816, publ. 03/07/2018).

Patent RU 2359455 C1, publ. 06/27/2009, refers to a biocidal composition that contains a polyguanidine compound, poly- (4,9-dioxadodecanguanidine chloride), or poly- (4,9-dioxadodecanguanidine phosphate), or poly- (4,9-dioxadodecanguanidine) succinate, or succinate polyhexamethylene guanidine, as a solvent contains water, or ethyl alcohol, or a mixture of ethyl alcohol and water, and also additionally contains benzalkonium chloride, sorbitol, an aqueous extract of stonecrop and α-hydroxy acid - glycolic, or apple, or citric acid.

Known QAC-based products have, as a rule, an unpleasant odor and irritating effect on mucous membranes, as well as low efficiency against fungi.

As the closest analogue, a disinfectant can be indicated, designed for quick and effective disinfection of surfaces, as well as for purification and deodorization of air in household premises and the general living environment, which has a pleasant aroma, the main components of which are ethyl alcohol, benzalkonium chloride, perfume composition and a hydrocarbon propellant. The perfume composition contains from 0.5 to 13 wt. % of essential oils such as rosemary, lavender, pine, rose (RU 2679603 C1, publ. 12.02.2019). The antiseptic and antibacterial effect of essential oils is noted.

The disadvantage of this agent is the irritating effect on the mucous membranes, the need for careful processing after application in order to eliminate the remnants of the agent and the lack of safety for the treatment of the skin.

The objective of the present invention is to create a special safe agent based on at least one quaternary ammonium compound (QAC) and 2-phenoxyethanol, where phytoextracts with phytoncidal activity against bacteria, viruses and fungi (including resistant regarding antimicrobial drugs).

The problem is solved by a new disinfectant that has a wide spectrum of action against antibiotic-resistant microorganisms, a high concentration of active substances and high storage stability.

The proposed composition of the disinfectant contains a quaternary ammonium compound (QAC) selected from benzalkonium chloride, 1,2-ethylene-bis- (N-dimethylcarbdecyloxymethyl) ammonium dichloride (ethonium) or mixtures thereof, 2-phenoxyethanol or its mixture with ethylhexylglycerol, at least at least one source of phytoncides, which is a phytoextract and / or essential oil of plants with a high content of terpenes, a structurant selected from Aristoflex (copolymer of acrylic acid), carbomer, sodium carboxymethylcellulose (blanose) or mixtures thereof, and water with the following content of components in wt. %:

HOUR 0.1-0.5 2-phenoxyethanol or its mixture with ethylhexylglycerol 0.5-4.0 source of phytoncides 1.0-10.0 structurant 0.01-0.6 Water rest

Phytoextracts and essential oils of plants with a high content of terpenes, such as cedar, pine, marigold, Leuzea Kubeba, eucalyptus, monarda, sage, calendula, lavender, oregano, bergamot, juniper, wormwood, etc. can be selected as a source of phytoncides. ...

As a result of using the proposed invention, the range of safe and highly effective agents with bactericidal, virucidal and fungicidal effects is expanding. This reduces the level of negative impact of chemicals and microbial aerosol in the air environment of a closed space on a person. Elimination of microbiological contamination and saturation of the indoor air with phytoncides has a prolonged effect: purity (99.9%) is achieved in 10-15 minutes and lasts from 4 to 6 hours. The tool can be used both in finished form and in the form of a working solution in dilution.

Preferred, but not limiting, particular cases of the invention are presented below.

Option 1, where the composition contains, by weight. %:

HOUR 0.1-0.5 2-phenxyethanol 0.7-1.0 Pine bud extract 0.5-2.5 Marigold essential oil 0.3-0.9 Leuzea Kubeba essential oil 0.2-0.5 Eucalyptus essential oil 0.05-1.5 Monarda essential oil 0.08-0.5 Aristolex (acrylic acid copolymer) 0.2-0.3 Water rest.

Option 2, where the composition contains, by weight. %:

HOUR 0.1-0.5 2-phenoxyethanol 0.7-1.0 Sage leaf extract 0.5-1.5 Eucalyptus essential oil 0.3-3.0 Sage essential oil 0.25-0.8 Pine essential oil 0.1-0.5 Carbomer 0.2-0.3 Blanosa 0.01-0.3 Water rest.

Option 3, where the composition contains, by weight. %:

HOUR 0.1-0.5 2-phenxyethanol 0.5-1.0 Oregano essential oil 0.5-1.0 Dandelion root extract 0.2-0.5 Calendula flower extract 0.2-1.5 Burdock root extract 0.1-0.5 Aristolex (acrylic acid copolymer) 0.2-0.3 Water rest.

Option 4, where the composition contains, by weight. %:

HOUR 0.1-0.5 2-phenoxyethanol 0.5-1.0 Ethylhexylglycerin 0.5-2.0 Calendula flower extract 0.5-1.5 Lavender essential oil 0.05-0.4 Leuzea Kubeba essential oil 0.1-0.5 Eucalyptus essential oil 0.05-0.5 Aristolex (acrylic acid copolymer) 0.2-0.3 Water rest

Option 5, where the composition contains, by weight. %:

HOUR 0.1-0.5 Phenoxyethanol 0.1-0.7 Sage leaf extract 0.5-1.5 Thyme essential oil 0.3-3.0 Cedarwood essential oil 0.25-0.8 Pine essential oil 0.1-0.5 Carbomer 0.2-0.3 Blanosa 0.01-0.3 Water rest.

D-panthenol and glycerin can also be added, preferably in the case of treating hands and other skin surfaces.

Option 6, where the composition contains, by weight. %:

HOUR 0.1-0.5 2-phenoxyethanol 0.5-1.0 Calendula flower extract 0.5-1.5 D-panthenol 0.05-0.1 Lavender essential oil 0.05-0.4 Leuzea Kubeba essential oil 0.1-0.5 Eucalyptus essential oil 0.05-0.5 Aristolex (acrylic acid copolymer) 0.2-0.3 Water rest.

Essential oils can be obtained from various parts of plants by known methods such as maceration, distillation, extraction, percolation and pressing.

Any known phytoextracts with a high terpene content can be contained as extracts: water, propylene glycol, carbon dioxide, water-alcohol.

The tool may additionally contain phytoextracts or essential oils of the Labiatae, Compositae families. These extracts are also high in terpenes, flavonoids and anthocyanins. The content of additional oils of the families Labiatae and / or Compositae can be, but not necessarily, from 0.1 to 10 wt. %, and extracts from 0.5 to 35 wt. %. Examples of Compositae extracts include extracts selected from the herb of the tripartite string, cornflower leaves, dandelion roots, burdock roots, yarrow, preferably in an amount of 0.7-12 wt. %, and extracts of plants of the Labiatae family - propylene glycol extract of Scutellaria Baikal roots, essential oil of medicinal rosemary.

Application methods:

1. Treatment of indoor air by aerosolization using trigger dispensers in a set of bottles.

2. Using any disinfection sprayer.

The result is achieved by the fact that when the proposed agent is sprayed through the nozzle of the dispenser, an aerosol of active microparticles is formed in the air space, which very quickly bind to the surface of microorganisms, due to their high adhesiveness. QAC, being a surface active substance, in combination with a structurant, has a high degree of sorption capacity for organic matter, which increases the level of binding to the surface of microorganisms. Further, there is a direct effect of active antimicrobial compounds on microorganisms (bacteria, viruses and fungi) and their inactivation. As a result, the cleanliness of the air in the room is achieved within 10-15 minutes and the effect can be maintained for 24 hours, depending on the intensity of air flows.

The product can be used locally by manual spraying.

The product removes unpleasant odors caused by the vital activity of microorganisms.

Essential oils have an additional immunostimulating, antioxidant, adaptogenic effect. Structuring agents, possessing sorbing properties, bind toxic substances.

As a result of the use of the proposed invention, the scope of application of the range of environmentally friendly highly effective disinfectants with immuno-strengthening, antimicrobial, antiviral and fungicidal effects is expanding.

Specific examples of implementation of the invention are presented below, in wt. % :.

Example 1.

Benzalkonium chloride 0.5 2-phenxyethanol 1.0 Pine bud extract 0.5 Marigold essential oil 0.5 Leuzea Kubeba essential oil 0.5 Eucalyptus essential oil 0.5 Monarda essential oil 0.1 Aristolex (acrylic acid copolymer) 0.3 Water rest.

Example 2.

Benzalkonium chloride 0.1 2-phenoxyethanol 0.7 Sage leaf extract 1.0 Eucalyptus essential oil 2.0 Sage essential oil 0.258 Pine essential oil 0.15 Carbomer 0.2 Blanosa 0.01 Water rest.

Example 3.

Etonius 0.5 2-phenxyethanol 1.0 Oregano essential oil 1.0 Dandelion root extract 0.2 Calendula flower extract 0.5 Burdock root extract 0.5 Aristolex (acrylic acid copolymer) 0.3 Water rest.

Example 4.

Etonius 0.3 2-phenoxyethanol 0.5 Ethylhexylglycerin 1.0 Calendula flower extract 1.5 Lavender essential oil 0.05 Leuzea Kubeba essential oil 0.1 Eucalyptus essential oil 0.05 Aristolex (acrylic acid copolymer) 0.2 Water rest.

Example 5.

Benzalkonium chloride 0.2 Phenoxyethanol 0.5 Sage leaf extract 1.5 Thyme essential oil 1.0 Cedarwood essential oil 0.8 Pine essential oil 0.1 Carbomer 0.1 Blanosa 0.3 Water rest.

Example 6.

Etonius 0.1 Benzalkonium chloride 0.2 2-phenoxyethanol 1.0 Calendula flower extract 1.5 D-panthenol 0.1 Lavender essential oil 0,4 Leuzea Kubeba essential oil 0.5 Eucalyptus essential oil 0.5 Aristolex (acrylic acid copolymer) 0.3

Water is the rest.

The finished product has a high bactericidal, fungicidal and viricidal activity. The product can be used to disinfect the air in hospitals belonging to the III-V categories (wards, medical and treatment rooms, reception rooms, infectious diseases hospital boxes, children's playrooms, vestibules, dirty laundry sorting rooms, smoking rooms, toilets) when bacterial (except mycobacterium tuberculosis) infections and influenza, as well as for preventive disinfection in health care facilities and at communal facilities (offices, beauty salons, hairdressers, baths, saunas, sports clubs, offices and reception areas of administrative institutions).

The activity of the disinfectant has been proven for the specified area of use by appropriate tests.

Example 7.

Testing the activity of the finished product form in the form of a liquid gel. Antibacterial activity was tested on strains of Staphylococcus aureus, Salmonella enteritidis, Pseudomonas aeruoginosa, Klebsiella pneumonia, Escherichia coli, Aspergillus niger, Candida albicans, etc., including antibiotic-resistant strains. Table 1 presents data on the study of 3 examples of formulations for clinical strains of Klebsiella pneumonia.

FIG. 1 shows a photograph of a plate with a culture of the Klebsiella pneumoni strain.

Antiviral activity is confirmed by a research act at the Institute. Gamalei.

The results on the activity against different types of microorganisms of the funds were obtained on the basis of the Federal State Institution "State Scientific Center for Applied Microbiology and Biotechnology" of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare of the Russian Federation, Obolensk.

The agent has antimicrobial activity against bacteria and viruses (including adenoviruses, influenza viruses, parainfluenza, bird flu, swine flu and other types of influenza viruses, causative agents of acute respiratory infections, enteroviruses, rotaviruses, poliomyelitis virus, viruses of enteral, parenteral hepatitis, herpes, atypical pneumonia, HIV infection, etc.).

The exposure time for air disinfection is 10-15 minutes.

Example 8.

Tests as a hand sanitizer according to SN EN 1500. Tests were performed using Escherichia coli ATCC 8739. The formulations of examples 1-6 were diluted with deionized water to a concentration of 3%. Propan-2-ol at a concentration of 60% by volume (v / v) was used as a comparison product. During the test, 3 ml of the agent and the reference product, respectively, were applied twice to the hands of ten subjects. The duration of the action was equal to 60 seconds for eight people and 30 seconds for two people. No neutralization was performed. The proposed formulations showed the same level of E. coli inactivation as the reference product, which fully complies with the requirements of the SN EN 1500 standard.

Retains its properties during freezing and subsequent thawing. That is, it shows high stability.

Toxicological indicators.

According to the parameters of acute toxicity, the disinfectant belongs to the 4th class of low-hazard substances according to GOST 12.1.007 when applied to the skin, injected into the stomach and intraperitoneally, and during inhalation.

Skin-irritating, skin-resorptive and sensitizing properties in the recommended modes of use of the agent have not been identified. The product has a moderate irritant effect on the mucous membranes of the eyes.

Claims (4)

1. Disinfectant containing a quaternary ammonium compound (QAC), characterized in that it additionally contains 2-phenoxyethanol or a mixture thereof with ethylhexylglycerol, at least one source of phytoncides, which is a phytoextract and / or an essential oil of plants selected from cedar, Scotch pine, marigolds, Leuzea Kubeba, eucalyptus, monarda, sage, calendula, oregano, juniper, wormwood, a structurant selected from Aristophlex, carbomer, sodium carboxymethyl cellulose or mixtures thereof, and water, and HAC is selected from 1.2 benzalkonium chloride, ethylene-bis- (N-dimethylcarbdecyloxymethyl) ammonium dichloride (ethonium) or mixtures thereof, with the following content of components, wt. %: HOUR 0.1-0.5 2-phenoxyethanol or its mixture with ethylhexylglycerol 0.5-4.0 Source of phytoncides 1.0-10.0 Structurizer 0.01-0.6 Water rest 2. The disinfectant according to claim 1, characterized in that it contains benzalkonium chloride as a QAC, and a mixture of pine bud extract, marigold essential oil, Leuzea Kubeba essential oil, eucalyptus essential oil, monarda essential oil as a source of phytoncides with the following content components, wt. %: Benzalkonium chloride 0.1-0.5 2-phenoxyethanol 0.7-1.0 Pine bud extract 0.5-2.5 Marigold essential oil 0.3-0.9 Leuzea Kubeba essential oil 0.2-0.5 Eucalyptus essential oil 0.05-1.5 Monarda essential oil 0.08-0.5 Aristolex (acrylic acid copolymer) 0.2-0.3 Water rest

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