JP2006111577A - Germicidal agent containing vegetable essential oil component and method for preventing infectious disease by using the germicidal agent - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain an agent exhibiting effects on prevention, or the like, of infectious diseases, and safely usable for a human being. <P>SOLUTION: The germicidal agent is obtained by formulating an essential oil component of a tea tree and an essential oil component of a mint so as to exhibit the germicidal activities against the whole of enteropathogenic Escherichia coli, Staphylococcus aureus and Legionella. Preferably, the germicidal agent is obtained by adding a prescribed amount or more of an essential oil component of a lavender so as to exhibit or enhance the germicidal activities against the Legionella. More preferably, the germicidal agent contains formulation components devised so as to be effective for mildewproofing by the addition, or the like, of an essential oil component of a rosemary. A method for preventing the infectious diseases involves spraying the germicidal agent on one of a human being, a target space and a target material. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a sterilization technique using a plant essential oil component as an active ingredient. More specifically, the present invention relates to a sterilization technique using a tea tree essential oil component, a mint essential oil component, a lavender essential oil component, a rosemary essential oil component, and the like.

  Currently, nosocomial infections in hospitals are a major problem. This nosocomial infection is said to be largely related to the behavior of nurses in each room. In addition to measures to prevent this, infection prevention for people living in the medical environment such as ambulances, health facilities, welfare facilities, etc. In addition, there is a need for a technology that can surely improve the effectiveness of infectious disease prevention in preventing infection of people who use many places and facilities such as baths, sports gyms, and leisure facilities.

  As a promising candidate for this technique, a technique of spraying or applying a disinfectant solution or the like to a desired place or space can be cited. Conventional spray techniques are not only for sterilization but also for deodorization and aromatherapy.

  Assuming the technology of spraying or applying the drug to a desired place or space, there is concern about effects on the human body such as mucous membrane irritation and respiratory tract irritation when inhaling the components of this drug and rough skin at the time of skin contact Since various components are desirable, various natural components have been studied. For example, bactericidal efficacy of plant essential oil components has been studied. Hereinafter, prior art relating to the sterilization or deodorizing effect of plant essential oil components will be described.

  Patent Document 1 discloses a technique that uses a medicine containing an essential oil component of tea tree oil and peppermint oil in an antibacterial deodorizing system for bedding and clothing.

  Patent Document 2 discloses an antibacterial agent containing a component selected from a peppermint essential oil component, a thyme essential oil component, an oak moss essential oil component, and the like, and discloses that such an antibacterial agent is effective against methicillin-resistant Staphylococcus aureus. Yes.

  Patent Document 3 discloses a deodorant cleaning agent containing a component selected from sage oil, thyme oil, basil oil, peppermint oil, mint oil, rosemary oil and the like.

  Patent Document 4 discloses a composition for preventing or treating oral candidiasis comprising an essential oil component such as tea tree oil, lavender oil, or rose oil as an active ingredient.

  Patent Document 5 discloses that extracts of lavender, rose plant, and the like function as antibacterial plant extract components, and discloses a technique of using these as an external preparation for skin.

  Patent Document 6 discloses a hypoallergenic antibacterial cosmetic using a solvent extract of Lamiaceae plants such as perilla and lavender as an antibacterial agent, and describes that there is almost no skin / mucosal irritation and high safety. ing.

  Patent Document 7 discloses an oral deodorant containing, as an active ingredient, essential oils extracted from plant seeds such as dill seeds and parsley seeds, rosemary leaves, and peppermint leaves.

Patent Document 8 discloses a deodorant composition containing at least a natural extract such as rosemary and sunflower seed and oxygen that oxidizes a phenolic compound.
JP 2000-271197 A. Japanese Patent Laid-Open No. 05-271064. JP 2000-212597 A. Japanese Patent Laid-Open No. 2003-089652. JP 2000-044419 A. JP 2001-172159 A. Japanese Patent Laid-Open No. 10-263064. Japanese Patent Laid-Open No. 10-212221.

  The present invention is effective for the prevention of infectious diseases and the like by clarifying the relationship between the plant essential oil component and the microbial species (mainly bacteria, mold) that the component can function as a bactericidal active ingredient, and safely An object is to provide a drug that can be used for personal use.

  The present invention is a medicine having a plant essential oil component as a bactericidal active ingredient, and the tea tree essential oil component and peppermint essential oil component are formulated so as to have a bactericidal action on all pathogenic Escherichia coli, Staphylococcus aureus and Legionella. Provide disinfectant. In this fungicide, the tea tree essential oil component and the peppermint essential oil component function as main active ingredients in relation to pathogenic Escherichia coli, Staphylococcus aureus, and Legionella. Other kinds of plant essential oil components and other bactericidal components or / and fungicidal components other than plant essential oil components can be freely added to this. In the present invention, “sterilization” is used as a concept including the concepts of antibacterial, antibacterial, and sterilization.

  For example, in addition to a tea tree essential oil component and a peppermint essential oil component, a bactericidal agent to which a predetermined amount or more of a lavender essential oil component is added so as to enhance the bactericidal action against at least Legionella bacteria is provided. Furthermore, a bactericidal agent devised for blending so as to be effective for mold prevention, for example, a bactericidal agent to which a rosemary essential oil component is added as an antifungal active ingredient. In addition, a rose essential oil component may be blended mainly for the purpose of enhancing the deodorizing action and relaxation action.

  Further, in the present invention, based on the newly found that the lavender essential oil component exhibits a strong bactericidal action on Legionella, at least the lavender essential oil component is contained in an amount that exhibits a bactericidal action on Legionella. Provide the agent. In this fungicide, the lavender essential oil component is the main active ingredient in relation to Legionella, and other types of plant essential oil components (for example, tea tree essential oil component, peppermint essential oil component, rosemary essential oil component) , Rose component, etc.) and other bactericidal components or / and fungicidal components other than plant essential oil components are free.

  The extraction method of the essential oil (essential oil) from the plant employ | adopted in this invention is not specifically limited. For example, known methods such as a steam distillation method, a pressing method, and a solvent extraction method can be appropriately employed.

  Next, in the present invention, there is provided an infectious disease prevention method characterized by spraying any one of the above bactericides to any one of a person, an object space, and an object.

  The spray device or spray device used for spraying the bactericide may be selected according to the purpose and is not particularly limited. Moreover, the target space and target part of spraying are not particularly limited. In the medical / nursing field and the living environment of each individual, for example, prevention of infection of people who use many places and facilities such as baths, sports gyms, leisure facilities, etc. Various places and parts can be mentioned, and the spray range may be determined according to the purpose or necessity. Moreover, the object to be sprayed is not particularly limited. Examples thereof include medical instruments and other instruments that may cause infection, clothing, wiping tools, articles such as shoes and sports equipment.

  The fungicide according to the present invention exhibits bactericidal action on all pathogenic Escherichia coli, Staphylococcus aureus, and Legionella fungi by the action of the tea tree essential oil component and the peppermint essential oil component. Moreover, the outstanding bactericidal action with respect to Legionella bacteria can be acquired reliably by using a lavender essential oil component as a main active ingredient, or using it as an additional compounding component.

  By spraying the disinfectant according to the present invention on a predetermined place or site, the occurrence or spread of infectious diseases can be effectively prevented. For example, spraying a fine mist containing a sterilizing active ingredient on a predetermined place and site is effective in preventing infection. Furthermore, since natural ingredients (plant essential oil ingredients) with low inhalation toxicity are used as active ingredients in the fine mist, anti-personal sterilization can be carried out safely, and the rough skin of the fingers and the like is caused by the moisturizing effect of the plant essential oil ingredients. Few.

  First, the inventors of the present application conducted a one-component verification test (Test 1) in order to verify the effects of the present invention. The procedure is as follows.

<1. Preparation of Escherichia coli and Staphylococcus aureus>
Colonies of Escherichia coli and Staphylococcus aureus cultured on a normal agar medium (LB agar medium) were collected and permeated overnight in an LB medium. Each bacterial solution was made 10 ⁹ cells / ml, 10 μl was taken and diluted with 5 ml of medium. 10 μl of this diluted solution was taken and diluted with 5 ml of medium. Further, 50 μl of this diluted solution (4,000 pieces / ml) was collected and applied to a previously prepared LB agar medium (5 cm dish) using a congealing stick.

<2. Preparation of Legionella coating medium>.
Legionella sp. Was cultured using BCYEα (a commonly used medium with carbon) and BSYE (antibiotic test medium). Legionella was cultured in advance on BCYEα and BSYE in a dish having a diameter of 90 mm and stored in a low temperature storage. Each medium of BCYEα and BSYE was prepared in a 50 mm diameter dish, and each stored Legionella colony was collected and applied to the surface of the medium using a platinum loop.

<3. Production of one-component disinfectant>.
In advance, 75% ethanol aqueous solution, 80% ethanol for disinfection, and 70% isopropanol aqueous solution were prepared as solvents for essential oils. A predetermined amount of each essential oil is 75% ethanol so that each of tea tree essential oil (abbreviated Tea), peppermint essential oil (abbreviated Mint), rosemary essential oil (abbreviated RM), and lavender essential oil (abbreviated LA) has a predetermined concentration. It was poured into an aqueous solution, 80% ethanol for sterilization, or an aqueous solution of 70% isopropanol and mixed by shaking. Each essential oil component dissolved in these solvents while having a slight cloudiness to obtain a one-component disinfectant containing each essential oil (see “Table 1” below for set concentrations). Then, these one-component disinfectants were put in a small glass spray container to prepare for spraying.

  Examples 1A to 1G are one-component fungicides containing tea tree essential oils at different concentrations, Examples 1H to 1K are one-component fungicides containing peppermint essential oil at different concentrations, and Examples 1L to 1O are rosemary. One-component fungicides containing essential oils in different concentrations, Examples 1P to 1T are one-component fungicides containing lavender essential oils in different concentrations. Note that the above three solvents were used as a control group. The control group 1 is a 75% ethanol aqueous solution, the control group 2 is an 80% ethanol for disinfection, and the control group 2 is a 70% isopropanol aqueous solution.

<4. Sterilization test>.
Various bacteria (Escherichia coli, Staphylococcus aureus, Legionella) were applied to each dish (two pieces each prepared). After 1 hour, each one-component fungicide and three kinds of solvents as a control group were 15 to 20 cm. The spray surface was sprayed directly from a remote location. Each dish was cultured overnight at 37 ° C., and the presence or absence of colony formation was observed the next day. In the case of Legionella, the cells were cultured at 37 ° C. for 5 to 6 days. Moreover, it verified about the effect with respect to white mold 1 and white mold. The used white mold 1 was collected from the roots of the houseplants placed indoors, and the white mold 2 was collected on the surface of the food jelly, transferred to a normal agar medium (LB agar medium), and proliferated. A part was applied to LB medium.

  Judgment of the bactericidal effect is ◎ (high effect: no colonies are recognized), ○ (medium effect: some colonies are recognized), △ (small effect: colonization is recognized), × (no effect: many The above colonies were observed).

  The results of this test are shown in the following “Table 1”. In Table 1, “Tea” indicates tea tree essential oil, “Mint” indicates peppermint essential oil, “RM” indicates rosemary essential oil, and “LA” indicates “lavender essential oil” (the same applies to other tables). ).

  As can be seen from the results shown in the above-mentioned “Table 1”, tea tree essential oil has a remarkable bactericidal effect on all of Escherichia coli, Staphylococcus aureus and Legionella at a concentration of 2.0% or more. And the growth of white mold 2 was suppressed. As for Escherichia coli, a bactericidal effect was observed at 0.5% or more.

  Peppermint essential oil has a remarkable bactericidal effect on all of Escherichia coli, Staphylococcus aureus, and Legionella at a concentration of 3.0% or more, and at the concentration of 2.0% or more, the growth of white mold 1 and white mold 2 is increased. Suppressed. Rosemary essential oil showed a bactericidal effect only on Escherichia coli at a concentration of 3.0% or more, and suppressed the growth of Shirofungi 1 and Shirofungi 2 at a concentration of about 5.0%.

  Lavender essential oil is found to have a very significant bactericidal effect against Legionella at a concentration of 2.0% or more, and at a concentration of 3.0% or more, it also has a bactericidal effect on Escherichia coli and Staphylococcus aureus. I found out. Further, at a concentration of about 3.0%, the growth of Shirofungi 1 and Shirofungi 2 was suppressed.

  As can be seen from the results of the control groups 1 to 3, the bactericidal effect of the alcohol solvent itself is very small or not at all, so that the bactericidal effect recognized in this "Test 1" is the plant essential oil. It can be said that it was brought about by the ingredients.

  As can be seen from the results of the control groups 1 to 3, the bactericidal effect of the alcohol solvent itself is very small or not at all, so the bactericidal effect recognized in this "Test 1" It can be said that it was brought about by the plant essential oil component.

  Next, an effect verification test (Test 2) using a fungicide (two-component fungicide) containing two types of plant essential oil components was performed. The procedure of “Test 2” was performed in accordance with the procedure of “Test 1” according to Example 1 above.

  In “Test 2”, in addition to Escherichia coli, Staphylococcus aureus and Legionella, the effect on white mold was also verified. The used white mold was collected from a homemade cocoon, transferred to a normal agar medium (LB agar medium), allowed to grow, and a part thereof was applied to the LB medium.

  The two-component fungicides used in this "Test 2" are Example 21 (tea tree essential oil 5%, peppermint essential oil 2.5%), Example 22 (tea tree essential oil 5.0%, rosemary essential oil 2. 5%), Example 23 (tea tree essential oil 5.0%, lavender essential oil 2.5%), and Example 24 (tea tree essential oil 3.0%, peppermint essential oil 2.0%). In this “Test 2” as well, the spray sterilization test was carried out for the control groups 1 to 3 (solvent only) in the same manner as “Test 1” described above.

  The results of this test are shown in the following “Table 2”. The determination method is the same as “Test 1” according to Example 1.

  As can be seen from the results shown in the above-mentioned “Table 2”, Examples 21 to 24 showed a sufficient bactericidal effect against Escherichia coli, Staphylococcus aureus, and Legionella, and also confirmed a bactericidal effect against white mold. Moreover, in Example 22 which mix | blended the rosemary essential oil component, the improvement of the mold prevention effect with respect to a mildew can be anticipated.

  As can be seen from the results of the control groups 1 to 3, the bactericidal effect of the solvent itself is very small or not at all. Therefore, the bactericidal effect recognized in this "Test 2" is also determined by the plant essential oil component. It can be said that it was done.

  Next, the effect verification test (Test 3) using the fungicide (multicomponent fungicide) containing the selected three or four kinds of plant essential oil components was performed. The procedure of “Test 3” was performed in accordance with the procedure of “Test 1” according to Example 1.

  In this "Test 3", in addition to Escherichia coli, Staphylococcus aureus (Mesitylin-resistant Staphylococcus aureus, MRSA is used for the four component system), Legionella, and Pseudomonas aeruginosa In addition, the effects on vancomycin-resistant enterococci (VRE), white mold (similar to test 2), black mold, yeast (Saccharomyces cerevisiae, budding yeast, D452-2 strain) were also verified.

  The culturing procedure for each bacterium was performed according to “Test 1” according to Example 1. For VRE, Todd Hewitt Broth medium was used. The yeast was subjected to liquid culture in a TPD medium, and then applied with a TPD agar medium and tested according to “Test 1” of a one-component system.

  The results of “Test 3” are shown in the following “Table 3”. The determination method is the same as “Test 1” according to Example 1. Examples 31-37 show three-component germicides, and Examples 41-44 show four-component germicides.

  As can be seen from the results shown in “Table 3” above, bactericides containing 2 to 3% tea tree essential oil and 2 to 3% peppermint essential oil are found in Escherichia coli, Staphylococcus aureus (including MRSA). A stable bactericidal effect is sufficiently and reliably exhibited, and a remarkable bactericidal effect is surely exhibited even for green-rich bacteria, VRE, Legionella bacteria, white mold, black mold and yeast. In the system containing lavender essential oil, the bactericidal effect against Legionella can be more surely considered in consideration of the result of “Test 1” of the one-component system.

  Based on the results of the above tests 1 to 3, sterilization formulated to show bactericidal action on all pathogenic Escherichia coli, Staphylococcus aureus, and Legionella by a basic blend composition combining tea tree essential oil and peppermint essential oil It is possible to provide an agent, and by adding a lavender essential oil component thereto, the bactericidal action against Legionella bacteria can be enhanced, and by adding a rosemary essential oil component, the fungicidal effect can be enhanced. Based on this, the selection and concentration setting of various essential oil components may be appropriately designed within the range where the bactericidal effect and fungicidal effect are exhibited in consideration of the necessary purpose and cost.

  Since it is possible to reliably remove pathogens that can cause infectious diseases from the desired space or place, or to suppress or prevent growth, it is not just a measure to prevent nosocomial infections that are currently problematic in hospitals, The living environment of each individual, such as when domestic and foreign travel and trekking cannot wash hands with water, and prevent infection of people working in medical settings such as ambulances, health facilities, welfare facilities, baths, sports gyms, leisure facilities, etc. It can also be used as a technology that can surely improve the effectiveness of infection prevention in the prevention of infection of people who use many places and facilities. In addition, it has been demonstrated that the alcoholic solvents currently used in clean rooms and other fields are weakly sterilized, so this technology is also effective as a sterilization technology for human bodies, as well as a sterilization and antifungal technology for various articles. It is. Furthermore, it can be widely used not only as a sterilizing action but also as a technique related to a deodorizing technique utilizing the aroma of an essential oil component, a relaxation by aroma spraying, an aromatherapy or the like.

Claims (6)

It is a drug containing a plant essential oil component as a bactericidal active ingredient,
A fungicide containing tea tree essential oil component and mint essential oil component so as to have a bactericidal action against all pathogenic Escherichia coli, Staphylococcus aureus and Legionella.   The fungicide according to claim 1, wherein a predetermined amount or more of a lavender essential oil component is added so that at least the fungicidal action against Legionella can be enhanced.   The disinfectant according to claim 1 or 2, which is also effective against mold.   The fungicide according to claim 3, wherein a rosemary essential oil component is added as an antifungal active ingredient. It is a drug containing a plant essential oil component as a bactericidal active ingredient,
A fungicide characterized in that at least the lavender essential oil component is contained in such an amount that it exhibits a bactericidal action against Legionella.   An infectious disease prevention method comprising spraying the bactericide according to any one of claims 1 to 5 to any one of a person, an object space, and an object.