RU2662322C1 - Cleaning and disinfecting agent - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention relates to the field of medicine, namely, to disinfectology and sanitation, and is intended for simultaneous cleaning and rapid disinfection from all known microorganisms of various solid non-porous surfaces or objects, including hard-to-reach ones. Cleaning and disinfecting agent includes n-propyl alcohol (propanol-1), alkyl dimethyl benzalkonium chloride, didecyldimethylammonium chloride, diethylene glycol monoethyl ether, tetrasodium salt ethylenediamine tetraacetic acid, ethoxylated fatty alcohol, 1H-benzotriazole, perfume composition and water. Components are used in claimed amounts.

EFFECT: use of the invention provides antimicrobial activity against Gram-positive and Gram-negative bacteria, fungi and viruses.

1 cl, 2 tbl

Description

The invention relates to medicine and can be used to obtain universal ready-made products for the simultaneous cleaning and rapid disinfection of all known types of microorganisms of various solid non-porous surfaces or objects, including hard-to-reach ones.

A disinfectant is known from RF patent No. 2475268, includes a quaternary ammonium compound, boric acid, and is an aqueous solution of a quaternary ammonium compound, which contains diethyl dichloropropenylammonium chloride or diethyldiallylammonium chloride in the following ratio of starting components,% May: said quaternary ammonium compound - 2% wt., Boric acid - 2.3% wt., Water - the rest. The invention provides an increase in bactericidal and fungicidal activity while reducing the concentration of working solutions of the drug, expanding the spectrum of action, including action on mycobacteria.

A disinfectant concentrate is known from Patent 2187460. The composition contains, by weight. %: hydrogen peroxide 35-40; soluble silver salt 1.0-2.0; aliphatic alcohol, mainly ethyl, isopropyl or propyl 0.05-1.0; water the rest. The disinfectant has a low corrosivity and a high disinfecting ability.

A disinfectant is known according to the patent of the Russian Federation No. 2286145 dated 10.27.2006, which contains alkyldimethylbenzylammonium chloride, benzotriazole, glutaraldehyde, monohydric alcohol (ethyl or isopropyl), hydrogen peroxide and water at a certain ratio of components.

The tool in accordance with the invention provides a high level of bactericidal, virucidal and fungicidal activity against a wide range of pathogens at relatively low concentrations of the working solution and a long shelf life and storage.

A disinfectant is known from patent 2593006, which consists of:

- from 25 to 32 wt. % mixture of ethanol, 1-propanol and 2-propanol, and it contains at least 5.0 wt. % and not more than 15 wt. % of each individual alcohol component,

- from 0.1 to 2.0 wt. % antimicrobial amphoteric surfactant (N-alkylaminopropylglycine) and from 66 to 71.9 wt. % water,

Disinfection includes the destruction of yeast, in particular Candida albicans, and / or the destruction of fungi, in particular Aspergillus brasiliensis, the disinfectant provides a decrease in the number of Candida albicans by 4 logarithmic units over 5 minutes in a quantitative test with a suspension of microorganisms, provides a decrease in the number of Aspergillus brasiliensis 4 logarithmic units for 15 min in a quantitative test with a suspension of microorganisms

From a patent of the Russian Federation 2535031 a disinfectant is known containing octenidine dihydrochloride (a surfactant having antimicrobial activity), propanol-1, propanol-2, citric acid, lavender essential oil and softened water. The invention provides the creation of a highly effective disinfectant with high antimicrobial activity against gram-positive and gram-negative bacteria, fungi of the genus Candida and Trichophyton, as well as viruses. The product is intended for the treatment of hands, the treatment of the skin of the surgical and injection fields of patients, the disinfection of gloves worn by the hands of medical personnel, the disinfection of hard-to-reach surfaces in food and catering enterprises.

The technical problem is the creation of a tool that provides simultaneous cleaning and quick disinfection of all known types of microorganisms, various solid non-porous surfaces or objects, including hard-to-reach ones.

The technical result consists in increasing the activity spectrum of a disinfectant with simultaneous high cleaning ability, low toxicity and the absence of corrosion activity in relation to the treated surfaces.

The problem is solved, and the technical result is achieved by the fact that the cleaning and disinfection agent contains n-propyl alcohol (propanol-1), a surfactant with antimicrobial activity, namely, alkyldimethylbenzylammonium chloride, didecyldimethylammonium chloride, and also diethylene glycol monoethyl ether, ethylene diamine tetraacetic acid, ethoxylated fatty alcohol, 1H-Benzotriazole, perfume composition and water in the following ratio of ingredients, wt.%:

normal propyl alcohol (Propanol-1) 10.0-19.0 alkyldimethylbenzalkonium chloride 0.10-0.20 didecyldimethylammonium chloride 0.10-0.20 diethylene glycol monoethyl ether 0.5-1.0 tetrasodium ethylenediamine tetraacetic acid 0.5-1.0 ethoxylated fatty alcohol 0.5-1.0 1H-Benzotriazole 0.02-0.04 perfume composition up to 0.2 water rest

The introduction of these ingredients together with n-propyl alcohol enhances antimicrobial activity.

The introduction of functional additives enhances the cleaning properties of the formulation:

- tetrasodium salt of ethylenediaminetetraacetic acid - complexing agent - includes in its structure and effectively removes impurities

- diethylene glycol monoethyl ether, an additional solvent, together with n-propyl alcohol provides dissolution and effective removal of organic contaminants

- ethoxylated fatty alcohol - non-ionic surfactant provides high washing / cleaning ability

- 1H-Benzotriazole provides anti-corrosion properties

Used, for example, the perfume composition "Alpine freshness" according to TU 9153-001-80507078-2007.

The combination of active antimicrobial components and functional additives creates an effective disinfectant with good cleaning properties and low corrosion activity in relation to the surfaces being cleaned. The balanced content of n-propyl alcohol in the composition contributes to the absence of fixation of organic impurities on the surfaces. The characteristics of the ingredients are shown in table 1.

Means for cleaning and disinfection are used for disinfecting surfaces and various objects by wiping or irrigation methods. The consumption rate of the product is not more than 50 ml / m2 (average 30-40 ml). The surfaces are irrigated with the product until completely wetted from a distance of 30 cm.

Wide antimicrobial spectrum of activity: it has a bactericidal activity against gram-positive and gram-negative bacteria, tuberculocidal (tested on Mycobacterium terrae), virucidal, fungicidal antimicrobial activity. The product actively destroys biological films on surfaces; has good detergent properties.

The method of preparation is mixing the components with constant stirring.

To obtain comparative data, the compositions of the claimed disinfectants were prepared (examples No. 1-5), shown in table 2.

The compositions were prepared as follows: water was added to n-propyl alcohol, and then the remaining components were added in turn, with constant mixing for at least 30 minutes.

Performance indicators were determined in accordance with Guidelines R 4.2.2643 “Methods of laboratory research and testing of disinfectants to assess their effectiveness and safety.

The antimicrobial effect of the drugs was studied in the finished product in parallel rows as follows:

1) Surfaces not contaminated with biological secretions were treated once. The treated surfaces and objects were irrigated from a distance of 30 cm until they were completely wetted (or wiped with a cloth preliminarily dampened with plenty of water) and left for the duration of exposure exposure.

Exposure time is 1 minute for any type of infection.

2) Surfaces contaminated with biological secretions were treated in two stages:

Stage 1: Cleaning the surfaces before disinfection: The product was applied directly to the surface to be cleaned. To remove dirt and biological contaminants (films), the surface was wiped with a clean disposable cloth

Stage 2: Disinfection of surfaces after cleaning is carried out in accordance with paragraph 1. The consumption rate of the product averaged 30-40 ml.

A study of the effectiveness of the drug was carried out on test surfaces using surfaces of 10 × 10 cm in size from various materials: tile, glass, artificial leather, metal, plastic, rubber.

S. aureus, E. coli, S. typhimurium, P. Aeruginosa, M. terrae, T. Gypseum and C. albicans, type 1 polio virus and type 5 adenovirus were used as test microorganisms.

The cultivation conditions of the test microorganisms: E. coli, S. typhimurium, P. aeruginosa and S. aureus - were cultured on the following nutrient media: casein broth, meat-peptone broth, Endo agar, casein agar, meat-peptone agar, etc. at a temperature of 37 ° C for 18-24 hours. A working culture of the test microorganism M. terrae was grown on an elective nutrient medium of Levenshtein-Jensen at a temperature of 37 ° C for 1-8 weeks.

The conditions for the cultivation of test mushrooms: C. albicans - on Saburo broth, Saburo agar at a temperature of plus 27 ° C for 2-10 days;

T. gypseum - on Saburo broth, Saburo agar at a temperature of plus 27 ° C for 28 days.

Museum cultures of the above microorganisms were stored at a temperature of plus (3 +/- 1) ° C in ampoules (after freeze drying) or on solid nutrient media (sowing by injection) under a layer of sterile liquid paraffin (layer thickness 1.5-3 mm), and work crops on mowed agar or in broth.

Before contamination with the test culture, the surfaces were subjected to mechanical cleaning - we were using soap and water with a brush.

The dried surfaces were placed horizontally, and a suspension of test microorganisms was applied on them with a pipette at the rate of 0.5 ml of 2 billion. microbial suspension on an area of 100 square meters. cm and evenly distributed it over the surface with a glass spatula. The surfaces were dried (until completely dry) at a temperature of 18-20 ° C and a relative humidity of 50-60%, then they were treated with an exposure time of 1 minute.

When studying the effectiveness of disinfection, artificial skin, glass were placed horizontally, and plastic, tile - vertically.

To simulate surface contamination, protein contamination was used: 40% inactivated serum.

The surface treatment was carried out by rubbing or irrigation by large-droplet and aerosol methods.

Control surfaces were treated with sterile drinking water also from the same calculation as the experimental ones.

Studies were carried out at a temperature of 18-20 ° C.

The effectiveness of disinfection of test surfaces was monitored as follows: a gauze cloth (5 × 5 cm in size) moistened with a solution of a universal neutralizer was thoroughly wiped with a test surface, then it was immersed in 10 ml of the same neutralizer in test tubes with beads. The time for washing the gauze napkin is 10 minutes. with constant shaking. The washing liquid was sown (2-3 cups, 0.1-0.2 ml each) on the corresponding solid differential diagnostic nutrient medium.

Depending on the type of test bacteria, crops were grown in a thermostat at a temperature of 37 ° C. The results were taken into account for 1-2 days by counting the number of colonies grown, and then the contamination density of 100 square meters was calculated. cm surface and% disinfection, taking the number of colonies removed from the control surfaces, for 100%.

Crops of test mushrooms were grown in a thermostat at a temperature of 28 ° C. The results were taken into account for 2-7 days (C. albicans, A) and 21-28 days (T. gypseum) by counting the number of colonies grown, then the density of contamination was calculated at 100 sq. see the surface and the percentage of disinfection, taking the number of colonies taken from the control surfaces as 100%.

The criterion for the effectiveness of disinfecting surfaces is at least 99.99% of the death of test microorganisms.

To determine the fixing action, test products made of metal (surgical scalpel pointed) and rubber from natural and synthetic rubber (fragments of drainage tubes 10 mm long) were used; glass (slide).

On the day of the experiment, on the surface of each test product (necessarily on the surface of the channel of the fragment of the drainage tube), two drops (diameter of at least 3 mm) of canned blood were applied using an eye or glass pipette (diameter of at least 3 mm) and dried until completely drying for 45-90 minutes. (depending on the humidity in the room).

The experiments are carried out at room temperature and the temperature of the solutions of the agent (active substance) (20 +/- 2) ° C.

Test items contaminated with blood, after drying, were immersed (avoiding contact with the edges of the container) in the product for various times (the maximum exposure time of the solution should not exceed 20 minutes).

After the exposure time, each test product is rinsed under running water (120 l / h) drinking water for 30 s, preventing a direct stream of water from reaching places originally contaminated with blood. The volume of water is measured using a measuring vessel and a stopwatch. Using a water tap, regulate the flow of water so that 1 liter of water flows into the measuring vessel in 30 seconds.

The presence of fixing properties of the active substances was judged by visually assessing the presence of blood residues on the surface of the test product in places where blood was applied after it was soaked in the agent and rinsed under running drinking water.

The cleaning ability of the compounds was determined according to OST 6-15-1660-90 "Household cleaning products. Methodology for determining the cleaning ability of products for cleaning enameled surfaces and glass. "

The corrosion effect of the samples was evaluated by measuring the size of the test surface samples before and after immersion in the test samples. The exposure during the dive was 1 month. And also, we evaluated under a microscope visual changes in structure, color, the appearance of rust, etc.

Disinfection efficiency

Studies have shown that compounds No. 1-5 have activity against test microorganisms: gram-positive and gram-negative bacteria, tuberculocidal (tested on Mycobacterium terrae), virucidal (poliovirus, adenovirus), fungicidal (including fungi of the genus Candida, Trichophyton ) antimicrobial activity.

Compositions No. 1-4 have a fixing effect, a weak fixing effect of composition No. 5 is due to the increased content of n-propyl alcohol.

The cleaning ability of the compositions No. 1, 2 - 80%, composition No. 3 - 86%, composition No. 4 - 90%.

Samples Nos. 3-5 were not corrosive to surfaces. The small corrosion activity of samples No. 1-2 is due to the low content of the anti-corrosion component.

Thus, the developed tool for cleaning and disinfection has antimicrobial activity against gram-positive and gram-negative bacteria, fungi, viruses. The product actively destroys biological films on surfaces; It does not fix organic pollution, has good detergent properties and low corrosivity in relation to cleaned surfaces.

Claims (2)

A cleaning and disinfecting agent, including n-propyl alcohol (propanol-1), surfactants having antimicrobial activity, characterized in that it includes alkyl dimethylbenzylammonium chloride, didecyldimethylammonium chloride and additional diethylene glycol monoethyl ether as surfactants ethylenediamine tetraacetic acid salt, ethoxylated fatty alcohol, 1H-Benzotriazole, perfume composition and water in the following ratio of ingredients, wt.%: Normal propyl alcohol (Propanol-1) 10.0-19.0 Alkyldimethylbenzalkonium chloride 0.10-0.20 Didecyldimethylammonium chloride 0.10-0.20 Diethylene glycol monoethyl ether 0.5-1.0 Ethylenediamine tetrasodium salt tetraacetic acid 0.5-1.0 Ethoxylated fatty alcohol 0.5-1.0 1H-Benzotriazole 0.02-0.04 Perfumery composition to 0.2 Water Rest