RU2297248C1 - Disinfecting agent - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention relates to agents used for disinfection. Disinfecting agent comprises quaternary ammonium compound and/or derivatives of polyhexamethylene guanidine, and/or N,N-bis-(3-aminopropyl)-dodecylamine, nonionogenic surface-active substance and salts of ethylenediaminetetraacetic or hydroxyethylenediphosphonic acids, and dye, perfume and water also wherein components are taken in the definite ratio, wt.-%. The proposed disinfecting agent provides high antibacterial properties, it shows good detergent properties, it stable in storage and safe in using in presence of humans and effective in using working solutions in the small concentrations.

EFFECT: valuable and effective properties of agent.

2 cl, 3 tbl, 17 ex

Description

The invention relates to means for disinfection in medical institutions (MPI); at ambulance stations, disinfection stations, in children's institutions, at public catering facilities, public utilities, in transport, in veterinary medicine, in agriculture, at food industry enterprises and in other sectors of the national economy, and the population in everyday life.

A disinfectant based on quaternary ammonium compounds (QAC) is known, for example, alkyl dimethylbenzylammonium chloride (ADBAH) and non-ionic surfactant - Maxi-Des, in which ADBAH is used in an amount of 4 wt.%, And as a nonionic surfactant (surfactant) ), which imparts detergent properties, ethoxylated nonylphenol (neonol AF 9/10) in an amount of 15 wt.%. However, the indicated disinfectant in working concentrations has weak detergent properties and a narrow spectrum of antimicrobial action [Methodical instructions for use for the disinfection of Maxi-Dez, Moscow, 2001].

Known disinfectant "Samarovka", in which as the active substance (AI) use a mixture of alkyldimethylbenzylammonium chloride and alkyldimethylethylbenzylammonium chloride in an amount of 9.6 wt.%. Despite the fairly high antimicrobial properties, the drug does not have an effective detergent effect (Methodological instructions for use and quality control methods of Samarovka for disinfection and pre-sterilization cleaning. Moscow, 2000).

Also known is the Dyulback DTBL disinfectant, in which quaternary ammonium compound (HOUR) - didecyldimethylammonium chloride in an amount of 5.0 wt.% Is also used as an active substance. The drug has a wide spectrum of action, however, resistant forms of bacteria require to inhibit the use of high concentrations - up to 8.0% for the drug (Guidelines for the use and quality control methods of the drug "Dyulbak DTBL" company PFH SNS, France, for disinfection and pre-sterilization purification. Moscow, 2000).

Closest to the proposed technical solution for the composition is a disinfectant according to the patent of the Russian Federation 2146151, A61L 2/16, 2000, containing (wt.%):

Alkyldimethylbenzylammonium chloride 10.0-12.0 Alkaline agent 5.0-10.0 Isopropyl alcohol 8.0-12.0 Water rest

As the alkaline agent, sodium bicarbonate or sodium carbonate, or sodium phosphate, or sodium acetate, or sodium thiocyanate, or sodium benzoate or urea are used. In addition, the disinfectant contains a non-ionic surfactant in an amount of 1-2%, which is used as ethoxylated alkyl phenols or fatty alcohols of the general formula RO (CH ₂ CH ₂ O) _X H, where RO is the residue of alkyl phenol or fatty alcohol, X = 7-20. However, this composition also has insufficient antimicrobial activity. In addition, despite the slightly alkaline environment and the presence of 1.0-2.0% nonionic surfactants, the drug exhibits weak detergent properties when treating surfaces in hospitals with working solutions. Its disadvantages include the content of a fairly large amount of isopropyl alcohol, which does not allow surface treatment in medical facilities in the presence of people due to the pungent odor. In addition, this drug refers to fire hazardous substances due to the presence of a large amount of isopropyl alcohol.

The objective of the invention was that based on the active substance, nonionic surfactants and functional additives to create an effective disinfectant with high antimicrobial and detergent properties

The problem is solved by using a composite composition containing in the optimal ratio the active substance, nonionic surfactant and water, and functional additives, including salts of ethylenediaminetetraacetic or hydroxyethylidene diphosphonic acid, as well as dye and perfume.

The proposed composition contains the following components, wt.%:

Active substance 1,0-20,0 Nonionic surfactant 1,0-20,0 Salts of ethylenediaminetetraacetic or hydroxyethylidene diphosphonic acid 2.0-10.0 Dye 0.001-0.1 Perfume 0.1-0.3 Water rest

The problem is solved in that, as an active substance, a compound selected from the range comprising a quaternary ammonium compound (QAC) and / or derivatives of polyhexamethylene guanidine and / or N, N-bis (3-aminopropyl) dodecylamine is used.

The problem is also solved by the use of non-ionic surfactants of oxyethylated alkyl phenols and / or oxyalkylated fatty alcohols of fractions C ₁₂ -C ₁₄ or C ₁₀ -C ₁₆ , and / or oxyethylated alkanolamides of fatty acids of fractions C ₁₂ -C ₁₄ or C ₁₀ -C ₁₆ .

As ethoxylated alkyl phenols, neonol AF 9/6, neonol AF 9/10, neonol AF 9/12 or mixtures thereof can be used. As oxyalkylated fatty alcohols of fractions C ₁₂ -C ₁₄ or C ₁₀ -C ₁₆ , syntanol DS-10, syntanol BV, syntanol ALM-7, syntanol ALM-10, syntanol DT-308 and others can be used. As ethoxylated alkanolamides, syntamide-5 or syntamide-10 can be used.

As a dye, one is used that is combined with the ingredients of the composition and does not impair the antimicrobial properties of the drug.

A mixture of nonionic surfactants and functional additives is used in the optimal ratio with the active substance, which allows to obtain a drug with higher antimicrobial activity, better washing properties compared to the prototype and high storage stability.

The following examples illustrate the invention.

Example 1

87.8 g of water with a temperature of 20-25 ° C are poured into a 4-necked flask with a stirrer, a thermometer and a reflux condenser, Neonol AF 9/10-2.0 g preheated to a temperature of 40-45 ° C is added, the solution is heated to 50-60 ° C and stirred for 15-20 minutes until completely dissolved. Then, at the same temperature and with constant stirring, the active substance is loaded - alkyl dimethylbenzyl ammonium chloride - 10.0 g, the mass is stirred until a homogeneous transparent solution is obtained, then the dye is added to the reaction mass - acid bright green anthraquinone Н4Ж - 0.002 g, stirred until uniform the colored solution, cooled to 20-25 ° C and add fragrance in an amount of 0.2 g. The composition of the obtained disinfectant is shown in table 1.

Example 2

82.8 g of water with a temperature of 20-25 ° C are poured into a 4-necked flask with a stirrer, a thermometer and a reflux condenser, 5.0 g of ethylenediaminetetraacetic acid tetrasodium salt are added and stirred until complete dissolution, after which it is preheated to temperature 40-45 ° С Neonol AF 9/10 - 2.0 g, the solution is heated to 50-60 ° С and stirred for 15-20 minutes. Then, at the same temperature and constant stirring, the active substance is loaded - alkyl dimethylbenzylammonium chloride - 10.0 g, the mass is stirred until a uniform transparent solution is obtained, then the dye is added to the reaction mass - acid bright green anthraquinone Н4Ж - 0.002 g, stirred until a uniformly colored solution, cooled to 20-25 ° C and add fragrance in an amount of 0.2 g. The composition of the obtained disinfectants are shown in table 1.

Example 3

A disinfectant is prepared according to example 1, but polyhexamethylene guanidine hydrochloride is used as the active ingredient, and direct yellow light-resistant O is used as a dye.

Example 4

The disinfectant is prepared according to example 2, but polyhexamethylene guanidine hydrochloride is used as the active ingredient, 2 g of ethylene diamine tetraacetic acid disodium salt is used as an activating agent, and direct yellow light-resistant O is used as a dye.

Example 5

The disinfectant is prepared according to example 1, but N, N-bis (3-aminopropyl) dodecylamine is used as the active ingredient, and acid green anthraquinone H ₂ C is used as a dye.

Example 6

The disinfectant is prepared according to Example 2, but N, N-bis (3-aminopropyl) dodecylamine is used as the active ingredient, 6.0 g of tetra disodium salt of ethylenediaminetetraacetic acid is used, and acid green anthraquinone H is used as a dye. ₂ S.

Examples 7-16

Disinfectants are prepared according to examples 1 and 2, but the components and their ratio are changed. The compositions of the obtained disinfectants are shown in table 1.

To confirm the feasibility of the invention and obtain the indicated technical result, comparative tests of drug samples for antimicrobial activity against strains of Pseudomonas aeruginosa were carried out, and the physicochemical and washing properties of the preparation were evaluated.

Example 17

Samples of the preparations according to the examples presented in table 1, were tested for antimicrobial activity against strains of Pseudomonas aeruginosa.

For tests on the antimicrobial activity of a disinfectant, only freshly prepared cultures of microorganisms are used. To prepare a microbial suspension containing a given number of microbial bodies per unit volume, 20-hour microbial cultures are used. To evaluate the effectiveness of a disinfectant sample, 100 µl of a disinfectant solution is added to 100 μl of microorganisms. Contamination time: 0 min, 15 min, 30 min, 60 min. As a control, inoculation of a bacterial suspension of the same dilution is used, treated with: a) a 5% solution of chloramine and b) without treatment. After incubation, the tubes are seeded with 50 μl of suspension and evenly distributed into plates with erythritol-agar (to determine the colony of the forming units — CFU after disinfection), which are also incubated in an incubator at 37 ° C for 24 hours. At the end of the incubation period, the number of colonies is counted and the degree of inhibition of the growth of microorganisms is estimated in comparison with the control samples. The test results are presented in table 2.

From the results shown in table 2, it follows that the disinfectants obtained in examples 1, 3, 5, 11 (without the addition of salts of ethylenediaminetetraacetic or hydroxyethylidene diphosphonic acids) are distinguished by a weak antimicrobial effect, close to the action of the prototype. Samples of disinfectants obtained in examples 2, 4, 6, 12, characterized by the introduction of salts of ethylenediaminetetraacetic or hydroxyethylidene diphosphonic acids have higher antimicrobial properties. When using several active substances (samples 7-10, 12-16), the effectiveness of antimicrobial action increases.

The physicochemical properties characterizing the effectiveness of the washing action were evaluated, and the washing effect was also evaluated by washing with a working solution containing 1.0% of a disinfectant, an artificially contaminated glass surface. A mixture of vegetable and animal fat was used as a pollutant. The test results for the determination of physico-chemical properties (activity of hydrogen ions - pH, foaming properties according to ISO 696-84, stability of 1% solutions), as well as washing properties, are presented in table 3.

As can be seen from the results shown in table 3, the disinfectant proposed according to the invention has the best physicochemical and washing properties in comparison with the prototype.

Thus, the proposed disinfectant has high antimicrobial properties, has good washing properties, is stable during storage, is safe when used in the presence of people, is effective when using working solutions in small concentrations.

Table 1 The composition of the disinfectant according to the invention Ingredients, wt.% Prototype according to the patent of the Russian Federation 2146151 Examples of the invention one 2 3 four 5 6 7 8 9 10 eleven 12 13 fourteen fifteen 16 Alkyldimethylbenzylammonium chloride 10.0 10.0 10.0 - - - 4.0 4.0 - 0.5 0.5 5.0- 8.0 4.0 - Alkyldimethylethylbenzylammonium chloride - - - 4.0 - - - - - - Didecyldimethylammonium chloride - - 16,0 4.0 4.0- 4.0 0.5 0.5 5.0- - 4.0 7.0 Polyhexamethylene guanidine hydrochloride - - 10.0 10.0 - - 5.0- - 1,0 1,0 - - N, N-bis (3-aminopropyl) dodecylamine 10.0- 10.0 4.0 7.0- 4.0 - 5,0 8.0 4.0 7.0 Neonol AF 9/10 2.0 2.0 2.0 5,0 5,0 6.0 6.0 - - 5.0- - 0.5 0.5 - - - Sintanol DS-10 - - - - - - - - 6.0 - Syntanol ALM-10 - - - 10.0 - 5.0- 3.0 - - 3.0 5,0 Sintanol DT-308 - - - - 10.0- 5.0- - - - - 8.0 Sintamide-5 6.0 6.0 - 10.0 - 3.0 0.5 0.5 - 6.0 - 8.0 Sintamide-10 - - - 5,0 - 5,0 - 3.0 5,0 Isopropyl alcohol 10.0 - - - - - - - - Urea 10.0 - - - Disodium EDTU - 2.0 - - - - 10.0- - Tetrasodium salt EDTU - 5,0 - 6.0 10.0 10.0 3.0 3.0- 4.0 - OEDP tetrasodium salt - - - 2.0 3.0 6.0 Dye - 0.002 0.002 0.002 0.002 0.002 0.002 0.003 0.003 0.003 0.002 0.001 0.001 0.002 0.002 0.002 0.003 Perfume 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.1 0.1 0.2 0.2 0.2 0.3 Water 68.0 87.8 82.8 84.8 82.8 77.8 71.8 54.8 57.8 49.8 79.8 96.9 94.9 75.8 67.8 74.8 63.7

table 2 The resistance of strains of Pseudomonas aeruginosa to the action of the disinfectants according to the invention at a concentration of 0,01% (active ingredient) Examples Contamination time, min, CFU right away 15 minutes 30 minutes 60 min According to the patent of the Russian Federation No. 2146151 drain growth drain growth 120 43 Example 1 drain growth drain growth more than 200 one hundred Example 2 drain growth 87 no growth no growth Example 3 drain growth drain growth 150 80 Example 4 drain growth 36 no growth no growth Example 5 drain growth 120 60 fifteen Example 6 25 no growth no growth no growth Example 7 no growth no growth no growth no growth Example 8 no growth no growth no growth no growth Example 9 no growth no growth no growth no growth Example 10 no growth no growth no growth no growth Example 11 drain growth 106 45 no growth Example 12 6 no growth no growth no growth Example 13 no growth no growth no growth no growth Example 14 no growth no growth no growth no growth Example 15 no growth no growth no growth no growth Example 16 no growth no growth no growth no growth

Table 3 Physico-chemical and washing properties of disinfectants according to the invention Test drugs Physicochemical properties Detergent properties of 1% working solution drug pH Stability of 1% solution (14 days) The stability of the foam 1% solutions,% According to the patent of the Russian Federation No. 2146151 7.85 cloudy after 2 hours 17.0 + According to example 1 8.1 transparent 62.0 + According to example 2 9.2 transparent 65.0 ++ For example 3 8.3 transparent 65.0 + For example 4 8.5 transparent 63.0 ++ For example 5 9,4 transparent 66.0 ++ For example 6 10,2 transparent 67.7 +++ For example 7 10.8 transparent 69.5 +++ For example 8 11.0 transparent 71.8 +++ For example 9 10.7 transparent 67.0 +++ For example 10 11.0 transparent 65.0 +++ For example 11 8.4 transparent 58.0 ++ For example 12 11.3 transparent 60.0 +++ For example 13 10.8 transparent 64.0 +++ For example 14 10.7 transparent 68.0 +++ For example 15 10.5 transparent 65.0 +++ For example 16 10.9 transparent 56.0 +++

Claims (2)

1. A disinfectant, including the active substance, nonionic surfactant and water, characterized in that as the active substance it contains a quaternary ammonium compound and / or a derivative of polyhexamethylene guanidine, and / or N, N-bis (3-aminopropyl) dodecylamine, and in as functional additives of the salt of ethylenediaminetetraacetic or hydroxyethylidene diphosphonic acids, dye and perfume in the following ratio of components, wt.%: Quaternary ammonium compound, and / or a derivative of polyhexamethylene guanidine, and / or N, N-bis (3-aminopropyl) dodecylamine 1,0-20,0 Nonionic surfactant 1,0-20,0 Salts of ethylenediaminetetraacetic or hydroxyethylidene diphosphonic acid 2.0-10.0 Dye 0.001-0.003 Perfume 0.1-0.3 Water Rest 2. The disinfectant according to claim 1, characterized in that it contains, as a nonionic surfactant, a mixture of oxyethylated alkyl phenol and / or oxyalkylated fatty alcohol of fractions C ₁₂ -C ₁₄ or C ₁₀ -C ₁₆ and / or oxyethylated alkanolamides of fatty acids of fractions C ₁₂ -C ₁₄ or C ₁₀ -C ₁₆ .