EA026955B1 - Disinfecting composition - Google Patents

Abstract

The invention discloses a disinfecting composition including a water-soluble salt of alkyl dimethyl benzyl ammonium; a water-soluble salt of polyhexamethylene guanidine; oxyethylidenediphosphonic acid or a water-soluble salt thereof, and a water-soluble salt of polydiallyldimethylammonium. Said composition can be used for water purification and for disinfection, in particular, for disinfection of various surfaces, equipment, ventilation and air conditioning systems. The composition according to the invention has an enhanced antiviral effect, enhanced flocculating properties, and work solutions thereof have elevated frost-resistance.

Description

The present invention relates to the field of sanitation and hygiene and, in particular, to a disinfectant composition for water purification and disinfection, in particular the disinfection of various surfaces, equipment, as well as ventilation and air conditioning systems.

State of the art

Currently, a large number of water purification agents are known. However, purified water does not always comply with generally accepted standards.

In the patent of the Russian Federation No. 2057796 a disinfecting composition for the treatment of bathrooms is proposed containing a mixture of alkylmethylbenzylammonium chloride with polyhexamethylene guanidine hydrochloride (0.023.0 wt.%), Nonionic surfactant (0.3-25.0 wt.%), Perfume (0.1-15 , 0 wt.%), Dye (0.001-0.25 wt.%) And water up to 100%.

The disadvantage of this tool is its insufficiently high antimicrobial activity against gram-negative and gram-positive bacteria, as well as storage instability, which consists in the separation of the colloidal solution.

To increase antimicrobial activity, RF patent No. 2208048 proposes a disinfectant composition containing alkylmethylbenzylammonium chloride (0.5-1.5 wt.%), Polyhexamethylene guanidine hydrochloride (1.5-3.0 wt.%) And an additive, in particular polydiallyldimethylammonium chloride (0.1-10 wt.%); nonionic surfactant - neonol, dye, perfume and water. It was shown that the introduction of polydiallyldimethylammonium chloride additive provides disinfecting properties for specific microorganisms: Ezhegyuhasa soy, Rzeyyoshoyaz aegidshoka, §1aryu1osossi8 aegeik and E1eogasac seloaasae.

This composition is one of the closest analogues of the claimed composition. However, this composition provides insufficient antiviral effect, and its working solutions do not have frost resistance.

The second of the closest analogues of the claimed composition is the composition proposed in RF patent No. 2297248. This composition contains a mixture of alkylmethylbenzylammonium chloride with polyhexamethylene guanidine hydrochloride (1.0-20.0 wt.%), Nonionic surfactant (1.0-20.0 wt. .%), hydroxyethylidene diphosphonic acid salt (2.0-10.0 wt.%), dye, perfume and water.

This composition when using working solutions in low concentrations has low toxicity to animals and humans. However, the disadvantage of this composition is also the lack of antiviral effect and the fact that its working solutions do not have frost resistance.

Also, these known compositions have insufficient flocculating properties, which does not allow their effective use for water purification.

Thus, the objective of the present invention is to overcome the disadvantages inherent in known compositions, namely the creation of a composition having a wide range of biocidal, including antiviral, enhanced flocculating properties and higher frost resistance of working solutions. Moreover, all these characteristics must be ensured while maintaining low toxicity to animals and humans.

SUMMARY OF THE INVENTION

The present invention provides a disinfectant composition comprising a water soluble salt of alkyldimethylbenzylammonium; water soluble salt of polyhexamethylene guanidine; hydroxyethylidene diphosphonic acid or a water soluble salt thereof and a water soluble salt of polydiallyldimethylammonium.

As was unexpectedly discovered by the authors of the present invention, the components of the composition exhibit synergism and provide a technical result consisting in enhancing the antiviral effect, enhancing flocculating properties and increasing frost resistance of working solutions. Moreover, this composition has low toxicity to animals and humans.

In one embodiment of the present invention, the ratio of alkyl dimethylbenzylammonium salt: polyhexamethylene guanidine or its hydroxyethylidene diphosphonic acid salt or its salt: polydiallyldimethylammonium salt in the composition is about 1: (0.3-30) :( 0.02-2) :( 0.01--2 1) by weight.

DETAILED DESCRIPTION OF THE INVENTION

Salts that make up the composition of the present invention can be any salt suitable for use in treating water and surfaces. In particular, such salts may be water soluble salts.

Salts of alkyldimethylbenzylammonium and polydiallyldimethylammonium can be halides, in particular chlorides.

Polyhexamethylene guanidine salts may be hydrohalide salts, in particular hydrochlorides.

The hydroxyethylidene diphosphonic acid salts may be metal salts, in particular alkali metals, such as sodium, potassium or ammonium salts.

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The components may be incorporated into the composition in any suitable amounts and ratios. So, the components can be introduced into the composition in an effective amount and at effective ratios. Such effective amounts and ratios may be amounts and ratios effective to provide the disinfecting effect of the composition and / or flocculating properties of the composition and / or frost resistance of the composition. The components can be incorporated into the composition in any desired amount and at any desired proportions. The amounts (contents) of the components of the composition and their proportions may be such that they do not prevent the disinfecting effect of the composition, in particular its antiviral effect, do not impair its flocculating properties and allow the working solutions to be frost-resistant. The contents of the composition and their proportions may be sufficient to ensure the disinfecting effect of the composition. The contents of the composition and their ratios may be sufficient to provide flocculating properties of the composition. The contents of the composition and their ratios may be sufficient to ensure frost resistance of the composition. By disinfecting action is meant, in particular, one or more disinfecting effects selected from antiviral, antibacterial, antifungal and / or any other action relating to disinfection, in particular any antimicrobial action.

In one embodiment of the present invention, the ratios of the components of the composition can be selected from the following ranges: alkyl dimethylbenzylammonium salt: polyhexamethylenetuanidine or a salt thereof: hydroxyethylidene diphosphonic acid or a salt thereof: polydiallyldimethylammonium salt of about 1: (0.3-30) :( 0.02-2 ) :( 0.01-1) by weight.

It is shown that at these ratios, the composition most effectively exhibits antiviral activity, flocculating effect and is the most frost-resistant.

In particular, the specified ratio of the components may be approximately 1: (0.3-3) :( 0.022) :( 0.01-1), 1: (0.3-3) :( 0.02-0.2) : (0.01-1), 1: (0.3-3) :( 0.02-0.2) :( 0.01-0.1), 1: (3-30) :( 0, 02-2) :( 0.01-1),

1: (3-30) :( 0.2-2) :( 0.01-1), 1: (3-30) :( 0.02-2) :( 0.1-1), 1: (3-30) :( 0.2-2) :( 0.1-1), 1: (0.3-3) :( 0.2-2) :( 0.01-0.1),

1: (0.3-3) :( 0.2-2) :( 0.1-1), etc.

In another embodiment of the present invention, the ratios of individual two or three components of the composition can be selected from these ranges, including the specific values and subranges included therein, and the content of the remaining components can be any. If necessary, the values can be converted to the unit of the required component.

In one embodiment of the present invention, the alkyl dimethylbenzylammonium salt is contained in an amount of about 1-10 wt.% Of the total weight of the composition, in particular in an amount of about 1-5 wt.%, 5-10 wt.% Or in an amount in any subrange from the indicated ranges.

In one embodiment, the polyhexamethylene guanidine salt is contained in an amount of about 3-30 wt.% Of the total weight of the composition, in particular about 3-15 wt.%, 15-30 wt.%, Or in an amount in any subrange of specified ranges.

In one of the embodiments of the present invention, hydroxyethylidene diphosphonic acid or its salt is contained in an amount of about 0.2-2 wt.% Of the total weight of the composition, in particular about 0.2-1 wt.%, 1-2 wt.%, Or an amount in any subrange of the indicated ranges.

In one embodiment of the present invention, the polydiallyldimethylammonium salt is contained in an amount of about 0.1-1 wt.% Of the total weight of the composition, in particular about 0.1-0.5 wt.%, 0.5-1 wt.%, Or in the amount located in any subrange of the specified ranges.

It will be apparent to those skilled in the art that all of these ranges include any specific values falling within those ranges, as well as any subranges.

The term approximately characterizes a possible deviation from the indicated values caused by certain circumstances. For example, the term approximately includes a possible error in determining the indicated values. Such an error may be, for example, ± 10% of the indicated values or another value, depending on the measurement method used.

The composition may contain solvents, carriers, diluents and excipients and the like. In particular, the composition may contain any suitable solvents, carriers, diluents and excipients and the like. The composition may contain one or more solvents, carriers, diluents and excipients and the like.

In particular, the composition may contain water.

In addition, the composition may contain additional components selected from the following: phosphonoacetic and nitrilomethylene phosphonic acids, polyacrylamide.

Moreover, all of these components may be contained in the composition in any suitable amount. So, all of these components can be contained in the required amount. All specified

- 2,026,955 components may be contained in sufficient quantities. The components may be contained in an amount necessary to bring the mass, volume or other characteristics of the composition to a certain value, including the amount necessary to bring the total percentage of components to 100%. All components can be incorporated into the composition in an effective amount. Said suitable, necessary, sufficient and effective amounts may be amounts suitable, necessary, sufficient and effective, respectively, to provide a disinfecting effect of the composition (including an action selected from an antiviral, antibacterial, antifungal and / or any other action related disinfection), and / or flocculating properties of the composition, and / or frost resistance of the composition.

Without limiting the scope of any particular theory, the inventors believe that the composition is based on the mechanism of formation of complex complexes between its components, while these complexes exhibit unexpectedly improved antiviral and flocculating properties, as well as increased frost resistance, compared with the corresponding characteristics of individual components.

In one embodiment of the present invention, the claimed composition has the following composition:

No. component Component Mass fraction,% one Alkyldimethylbeisylammonium chloride 1-10 2 Polyhexamethylene guanidium hydrochloride 3-30 3 Sodium salt of oxyetgishdsnediphospho1 your acid 0.2-2 4 Polydiallyldimethylammonium chloride 0.1-1 5 Water Up to 100

The compositions of the present invention can be used to purify water.

In addition, the compositions according to the present invention can be used, in particular: in medical institutions (including pediatric and neonatology departments), infectious foci during prophylactic, current and final disinfection for disinfection of surfaces in rooms, furnishings, surfaces of devices, appliances, sanitary equipment, patient care items, personal hygiene products, toys, linen, dishes (dining room, laboratory, pharmacy), items for washing the courts; shoes made of polymeric materials, rubber and polypropylene mats, cleaning equipment, waste disposal systems, medical waste from textile and other materials (dressings, cotton-gauze napkins, tampons, disposable medical supplies and disposable underwear), urine, fecal urinary suspension, blood, disinfection of incubators and accessories to them; component parts of anesthesia-respiratory equipment, anesthesiology equipment; disinfection on medical vehicles during general cleaning;

in public utilities (hotels, hostels, hairdressers, baths, laundries, markets, public toilets), in educational, cultural, recreational, sports (sports and recreation centers, swimming pools, cinemas, offices, etc.), social security institutions, child care facilities, public catering and trade enterprises for the preventive disinfection of indoor surfaces, furnishings, surfaces of apparatuses, appliances, vehicles for transporting food , sanitary equipment, rubber and polypropylene mats, garbage collection equipment, garbage containers, garbage trucks, shoes made of polymeric materials, tools (hairdressers and cosmetics), dishes, items for washing dishes, personal hygiene products, linen, cleaning equipment;

for disinfection of special equipment, workwear, tools in hairdressing salons, massage parlors, beauty salons;

for preventive disinfection of surfaces in rooms, furnishings, external surfaces of devices and apparatuses at the enterprises of the pharmaceutical and biotechnological industry for the production of non-sterile medicines in rooms of cleanliness classes C and O;

disinfection of surfaces affected by mold;

disinfection of ventilation and air conditioning systems (domestic and industrial air conditioners, split systems, multi-zone split systems, roof air conditioners, etc.).

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Examples

Several series of experiments were conducted aimed at obtaining the claimed composition at various ratios of components and comparing the effects of the claimed composition with the closest analogues known in the art.

Example 1

Obtaining the claimed composition.

Received various variants of the claimed composition by mixing alkyldimethylbenzylammonium chloride; polyhexamethylene guanidine hydrochloride; sodium salt of hydroxyethylidene diphosphonic acid and polydiallyldimethylammonium chloride in various ratios of components.

The resulting composition, if necessary, was diluted with water.

As an example, we can consider several compositions, the composition of which is presented in table. one.

Table 1

The composition of the compositions

Marking composition Component Content, mass % Ratio components by weight Composition 1 Alkyldimethylbenzylammonium chloride one 1: 30: 2: 0.1 Polyhexamethylene guanidine hydrochloride thirty Sodium salt oxyethylene diphosphonic acid 2 Poured allyldimethyl ammonium chloride 0.1 Water 57 Composition 2 Alkyldimethylbenzylammonium chloride 5 1: 3: 0.2: 0.1 Polyhexamethylene guanidine hydrochloride fifteen Sodium salt oxyethylene diphosphonic acid one Polydiallyldimethyl ammonium chloride 0.5 Water 78.5 Composition 3 Alkyldimethylbenzylammonium chloride 10 1: 0.3: 0.02: 0.1 Polyhexamethylene guanidine hydrochloride 3 Sodium salt oxyethylene diphosphonic acid 0.2 Polydiallyldimethyl ammonium chloride one Water 85.8

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Composition 4 Alkyldimethylbenzylammonium chloride 10 1: 0.3: 0.02: 0.01 Polyhexamethylene guanidine hydrochloride 3 Oxyethylene diphosphonic sodium salt acid 0.2 Polydiallyldimethylammonium chloride 0.1 Water 86.7 Composition 5 Alkyldimethylbenzylammonium chloride I 1: 30: 2: 1 Polyhexamethylene guanidine hydrochloride thirty Sodium salt oxyethylene diphosphonic acid 2 Polydiallyldimethylammonium chloride one Water 66

Example 2

Comparison of the effects of the claimed composition with the action of its closest analogues known from the prior art, and individual components

In the experiments conducted, the initial characteristics of the water samples were evaluated, as well as the characteristics of the water samples after treatment with various reagents, in particular:

a composition containing as active components alkylmethylbenzylammonium chloride, polyhexamethylene guanidine hydrochloride and polydiallyldimethylammonium chloride, as described in RF patent No. 2208048 (hereinafter, the composition according to RF patent No. 2208048);

a composition containing, as active components, alkylmethylbenzylammonium chloride, polyhexamethylene guanidine hydrochloride and a salt of hydroxyethylidene diphosphonic acid, as described in RF patent No. 2297248 (hereinafter, the composition according to RF patent No. 2297248);

hydroxyethylidene diphosphonic acid sodium salt (a component distinguishing the claimed composition from the composition according to the patent of the Russian Federation No. 2208048);

polydiallyldimethylammonium chloride (a component that distinguishes the claimed composition from the composition according to the patent of the Russian Federation No. 2297248),

For all these samples, the antiviral effect (by coliphages), flocculating properties (by turbidity) and frost resistance of working solutions were evaluated. The biocidal effect was also evaluated (by the number of common coliform bacteria, thermotolerant coliform bacteria, spores of sulfite-reducing clostridia and lamblia cysts).

Below is a detailed description of the experiments and the results obtained (see table. 2-4 below) on the example of processing a water sample characterized by a turbidity of 4.5 mg / l;

the number of coliphages 12 plaque-forming units in 100 ml; the number of total coliform bacteria 146 in 100 ml; the number of thermotolerant coliform bacteria 8 in 100 ml; the number of spores of sulfite-reducing clostridia 3 in 20 ml and the number of lamblia cysts 4 in 50 ml.

Antiviral study

The antiviral effect of known compositions, individual components and the claimed composition was evaluated by the number of coliphages for compliance with the indicators given in SanPiN 2.1.4.559-96.

The studied water sample (100 ml) and a Petri dish with E. E. control were placed in a thermostat for 20 hours at a temperature of 37 ° C.

To control the culture, 0.1 ml of bacteria washing E.soi (or 0.2 ml of a 4-hour broth culture) was placed in a Petri dish and filled with nutrient agar.

After incubation from the test sample, 10 ml was poured into a test tube and 1 ml of chloroform was added. The tube was closed with a sterile rubber or silicone stopper, shaken vigorously and left at room temperature for at least 15 minutes until complete chloroform precipitation.

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The pre-melted E.coH bacteria wash was added to the pre-molten and cooled to 45-49 ° C nutrient agar at the rate of 1.0 ml of wash per 100 ml of agar. To determine coliphages, 1 ml of a sample treated with chloroform was pipetted from a test tube into a sterile Petri dish and filled with a mixture of nutrient agar and cooled to 45-49 ° C, 12-15 ml of volume, as well as one additional Petri dish to control the culture of E. soi, were mixed and left on the table until the agar completely solidified, then the Petri dishes were placed in a thermostat for 18 ± 2 hours at 37 ° C.

View crops carried out in transmitted light. The sample was considered positive in the presence of complete lysis, enlightenment of several plaques, one plaque on the plate with a sample of water in the absence of lysis zones on the control plate. In the presence of lysis zones in the control, the result was considered invalid.

The results are shown in table. 2.

table 2

Evaluation results of the antiviral effect of known compositions, individual components and the claimed composition

Treatment Coliphages, plaque forming units in 100 ml Water sample before processing 12 The composition according to the patent RF> 2208048 2 The composition according to the patent RF number 2297248 4 Hydroxyethylidene diphosphonic acid 12 Polydiallyldimethylammonium chloride 4 The claimed composition: Composition 1 Are absent Composition 2 Are absent Composition 3 Are absent Composition 4 Are absent Composition 5 Are absent

As can be seen from the table. 2, the components of the claimed composition demonstrate an unexpected synergistic antiviral effect, which allows to achieve results that cannot be achieved when using known compositions or individual components that distinguish the claimed composition from the known.

So, from the table. 2 shows that compositions containing three of the four claimed components, i.e. the compositions according to the patents of the Russian Federation No. 2208048 and 2297248, do not have a sufficiently high antiviral effect: they reduce the average number of coliphages from 12 to 2 and from 12 to 4 plaque-forming units in 100 ml, respectively.

Moreover, the fourth component that is absent in each of these compositions, but present in the composition according to the present invention (in this case, hydroxyethylidene diphosphonic acid and polydiallyldimethylammonium chloride, respectively), taken separately, does not provide the best effect: hydroxyethylidene diphosphonic acid does not exhibit any antiviral effect, but polydiallyldimethylammonium chloride reduces the average number of coliphages from 12 to 4 plaque forming units in 100 ml, respectively.

At the same time, a composition containing all four components unexpectedly reduces the number of coliphages to their complete absence, which was not achieved when using the composition of the three components, or when using the fourth component separately.

Thus, it is not obvious to a person skilled in the art that a composition containing all four components will work significantly better than a composition of three components or a fourth component taken separately.

Assessment of biocidal action against coliform bacteria, thermotolerant coliform bacteria, spores of sulfite-reducing clostridia and lamblia cysts.

The determination of total coliform bacteria, thermotolerant coliform bacteria, sulphite-reducing clostridia and lamblia cysts was also determined for compliance with the indicators given in SanPiN 2.1.4.559-96.

The method for determining coliform bacteria in water by the method of membrane filters.

The filter apparatus was wiped with a cotton swab moistened with alcohol and flamed. After cooling, they placed on the lower part of the filter apparatus (table) with flaming tweezers

- 6 026955 sterile membrane filter, pressed it with the upper part of the device (glass, funnel) and fixed with the device provided by the design of the device.

A precisely measured volume of water was poured into the upper part of the device, then a vacuum was created in the lower part of the device.

When filtering 1 ml of the test water or diluting it, at least 10 ml of sterile tap water was pre-poured into the funnel, and then the test water was added.

After the filtration was completed, the funnel was removed, the filter was carefully lifted by flaming tweezers over the edge while maintaining a vacuum to remove excess water on the underside of the filter, and then it was transferred without turning onto a nutrient medium poured into Petri dishes, avoiding air bubbles between the medium and the filter. The surface of the filter with bacteria settled on it should be facing up.

3-4 filters were placed on one cup, provided that the filters were not in contact.

The analysis was carried out in 3 samples of 100 ml. A precisely measured volume of water was filtered through membrane filters in compliance with the above requirements.

The filters were placed on Endo medium, placed in a thermostat upside down, and the crops were incubated at a temperature of (37 ± 1) ° С for 24 ± 2 hours.

The result was considered negative if colonies did not grow on the filters at all or colonies with uneven edges and surfaces (membranous, spongy, moldy, transparent, mucous) grew.

With drain growth, all filters were sieved on Endo medium to obtain isolated colonies by conventional bacteriological methods.

In the presence of typical lactose-positive colonies giving an imprint on the reverse side of the membrane filter and the medium — dark red, red with and without metallic luster, and lactose-negative — pink without fingerprints, the number of colonies of each type was counted.

At least 5 colonies of each species were selected for identification, they were sown on mowed agar, and then biochemical tests were studied. The oxidase test and the acid and gas formation test during the fermentation of lactose or mannitol (glucose) were used as confirmatory tests.

To determine the oxidase activity, 2-3 drops of a freshly prepared oxidase test reagent were placed on filter paper. Prepared papers are moistened with distilled water. A smear of fresh culture was placed on a prepared paper with a glass rod. The reaction was considered positive if a violet-brown or blue color appears within 10-30 seconds.

The cultures that gave oxido-positive reactions were not subject to further investigation, since common coliform bacteria include gram-negative, non-spore-forming rods, not oxidase-active, fermenting lactose or mannitol with the formation of aldehyde, acid and gas at a temperature of 37 ° C for 24 hours .

Thermo-tolerant coliform bacteria are indicators of fresh fecal contamination and have all the signs of common coliform bacteria, which are also capable of fermenting lactose to acid and gas at a temperature of 44 ° C for 24 hours.

All typical lactose-positive colonies were seeded in confirmation media with lactose and mannitol and incubated for 24 hours at 37 ° C to determine the total coliform bacteria.

To determine the thermotolerant bacteria, sowing was carried out in a medium preheated to a temperature of 44 ° C, and incubation was carried out at the same temperature for 24 hours.

Colonies were considered as common coliform bacteria in the negative oxidase test, fermentation of lactose or mannitol (glucose) at a temperature of 37 ° C with the formation of acid and gas. Among these colonies, thermotolerant coliform bacteria were taken into account during the oxidase test and lactose fermentation at a temperature of 44 ° C with the formation of acid and gas.

If, during a random check of colonies of the same type, different results were obtained, then to calculate the number of coliform bacteria among these colonies, the formula (a x c) is used

X .------ b where x is the number of colonies of the same type; a - the total number of colonies of this type;

B is the number of verified ones; c is the number of colonies with a positive result.

Calculation and presentation of results.

The result of the analysis was expressed by the number of colony forming units (CFU) of total coliform bacteria in 100 ml of water. To calculate the result, the number of colonies was confirmed, confirmed as common coliform bacteria grown on all filters, and divided by 3.

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Determination of the total number of microorganisms forming colonies on nutrient agar.

This method was used to determine the total number of mesophilic aerobic and facultative anaerobic microorganisms (total microbial number, TBC) capable of forming colonies on nutrient agar at 37 ° C for 24 hours, visible with a 2-fold increase.

From each sample of the selected water, at least two volumes of 1 ml were sown. After thoroughly mixing the water samples, 1 ml was added to sterile Petri dishes; immediately, 6–8 ml of melted and cooled nutrient agar was poured into each cup at 45–46 ° С. Then mixed the contents of the cups, evenly distributing throughout the bottom, avoiding the formation of air bubbles, getting agar on the edges and lid of the cup.

After agar solidification, the inoculated plates were placed in a thermostat at a temperature of 37 ° C for 24 hours.

All colonies grown on the plate were counted, observed with a 2-fold increase. Counting was carried out only on those dishes on which no more than 300 isolated colonies grew.

The calculated number of colonies on each plate was summarized and divided by two. The result was expressed as the number of colony forming units (CFU) in 1 ml of the test water sample.

The results are shown in table. 3.

Table 3

The results of the evaluation of the biocidal action of known compositions, individual components and the claimed composition

Treatment Index Are common coliform ny bacteria, the number of bacteria in 100 ml Thermo-tolerant coliform bacteria, the number of bacteria in 100 ml Disputes sulfitre- I am leading of people clostridium, the number of spores in 20 ml Giardia cysts, the number of cysts in 50 ml Water sample before processing 146 8 3 4 The composition according to the patent RF № 2208048 12 one one one The composition according to the patent RF number 2297248 23 2 one 2 Hydroxyethylidene diphosphonic acid 146 8 3 4 Polydiallyldimethylammonium chloride 34 2 one 2 The claimed composition: Composition 1 Are absent Are absent Are absent Are absent Composition 2 Are absent Are absent Are absent Are absent Composition 3 Are absent Are absent Are absent Are absent Composition 4 Are absent Are absent Are absent Are absent Composition 5 Are absent Are absent Are absent Are absent

As can be seen from the table. 3, the components of the claimed composition demonstrate an unexpected synergistic biocidal effect, which allows to achieve results that cannot be achieved when using known compositions or individual components that distinguish the claimed composition from the known.

So, from the table. Figure 3 shows that compositions containing three of the four claimed components, i.e. compositions according to the patents of the Russian Federation No. 2208048 and 2297248, do not have a sufficiently high biocidal effect.

Moreover, the fourth component that is absent in each of these compositions, but present in the composition according to the present invention (in this case, hydroxyethylidene diphosphonic acid and polydiallyldimethylammonium chloride, respectively), taken separately, does not provide the best effect.

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At the same time, a composition containing all four components unexpectedly reduces the number of bacteria to their complete absence, which was not achieved when using the composition of the three components, or when using the fourth component separately.

Thus, it is not obvious to a person skilled in the art that a composition containing all four components will work significantly better than a composition of three components or a fourth component taken separately.

The study of flocculating action.

The flocculating effect was investigated by determining the turbidity of the water sample before and after treatment.

Turbidity was determined by the photometric method according to GOST 3351-74.

The results are presented in table. 4.

Table 4

The results of the evaluation of the flocculating effect of known compositions, individual components and the claimed composition

Treatment Turbidity of the sample, mg / l Water sample before processing 4,5 The composition according to the patent RF № 2208048 1.6 The composition according to the patent RF number 2297248 3.2 Hydroxyethylidene diphosphonic acid 4,5 Polydiallyldimethylammonium chloride 1.8 The claimed composition: Composition 1 0.57 Composition 2 0.43 Composition 3 0.62 Composition 4 1,2 Composition 5 0.34

As can be seen from the table. 4, the components of the claimed composition demonstrate an unexpected synergistic flocculating effect, which allows to achieve results that cannot be achieved when using known compositions or individual components that distinguish the claimed composition from the known.

So, from the table. Figure 4 shows that compositions containing three of the four claimed components, i.e. compositions according to the patents of the Russian Federation No. 2208048 and 2297248, do not have a sufficiently high flocculating effect.

Moreover, the fourth component that is absent in each of these compositions, but present in the composition according to the present invention (in this case, hydroxyethylidene diphosphonic acid and polydiallyldimethylammonium chloride, respectively), taken separately, does not provide the best effect.

At the same time, a composition containing all four components unexpectedly reduces turbidity to values that were not achieved when using the composition of the three components, or when using the fourth component separately.

Thus, it is not obvious to a person skilled in the art that a composition containing all four components will work significantly better than a composition of three components or a fourth component taken separately.

Study of frost resistance of working solutions.

Also, for the above compositions and individual components, the frost resistance of the working solutions was evaluated.

The evaluation was carried out as follows.

The sample was placed in a refrigeration chamber for a day, if after this period of time a precipitate did not form, the temperature was lowered by 1 ° C and kept again for 24 hours.

The temperature at which a precipitate formed was determined.

The results are presented in table. 5.

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Table 5

The results of the evaluation of frost resistance of known compositions, individual components and the claimed composition

Composition or single reagent Stability of working solutions The composition according to the patent RF № 2208048 Stable with positive temperatures The composition according to the patent RF number 2297248 Stable with positive temperatures Hydroxyethylidene diphosphonic acid Stable with positive temperatures Polydiallyldimethylammonium chloride Stable with positive temperatures The claimed composition: Composition 1 Stable up to -7 ° С Composition 2 Stable up to -7 ° C Composition 3 Stable up to -7 ° C Composition 4 Stable up to -7 ° С Composition 5 Stable up to -7 ° С

As can be seen from the table. 5, the components of the claimed composition demonstrate an unexpected synergistic effect, providing frost resistance of working solutions. Moreover, as can be seen from the table. 5, working solutions of known compositions or individual components that distinguish the claimed composition from the known ones do not have frost resistance: a precipitate formed at temperatures below 0 ° C.

So, from the table. 5 that compositions containing three of the four claimed components, i.e. compositions according to the patents of the Russian Federation No. 2208048 and 2297248, do not have frost resistance.

Moreover, the fourth component that is absent in each of these compositions, but present in the composition according to the present invention (in this case, hydroxyethylidene diphosphonic acid and polydiallyldimethylammonium chloride, respectively), taken separately, does not provide the best effect.

At the same time, a composition containing all four components unexpectedly provides frost resistance, which was not achieved when using the composition of the three components, or when using the fourth component separately.

Thus, it is not obvious to a person skilled in the art that a composition containing all four components will work significantly better than a composition of three components or a fourth component taken separately.

Assessment of the effectiveness of surface disinfection.

To compare the compositions and individual components according to the effectiveness of surface disinfection, the minimum inhibitory concentration was determined according to the most common model strains. The smaller it is, the more effective the composition or individual component.

In the study of minimal inhibitory concentrations against bacteria and fungi, the method of serial dilutions in liquid media was used.

Trypcase-soy broth (Tgursake 8ou ΒγοΙΠ) from the company Lewspssih was used as a liquid nutrient medium for growing bacteria. France, and for the cultivation of representatives of the mold flora, Capeque’s liquid medium. According to this method, 2 ml of liquid culture medium was poured into 17 sterile tubes. 2 ml of stock solution was added to the first tube. The contents were mixed and 2 ml of the solution was transferred to a second tube and so on until the 15th tube, from which 2 mm was removed.

Only a suspension of test microorganisms was added to the 16th test tube, and it served as a control of the growth of microorganisms.

Nothing was added to the 17th test tube, and it served as a control of the sterility of the culture medium.

In all tubes except the 17th, 0.2 ml of culture was added with a test of the microorganism. Crops with bacterial cultures were incubated in an incubator at 37 ° C for 24 hours, and mold cultures at 29 ° C for 10 days. The results were taken into account in the presence of the growth of microorganisms in the control culture and the absence in the control environment. Then the last tube was noted with a complete visible growth retardation of microorganisms. This dilution was the minimum inhibitory concentration for the test strain and determined the degree of its bacteriostatic activity for this drug.

To establish bactericidal activity from all non-germinated (i.e., which did not show visible growth of test microorganisms) tubes with liquid nutrient media and two germinated (as

- 10,069,955 controls) using a bacteriological loop, seeding was performed on solid nutrient media (Tgursake 8ou Adag, YoMepeih firm. France and апapek agar). Crops with bacterial cultures were placed in a thermostat at 37 ° C for 24-72 hours, and with mold cultures at 29 ° C for 10-14 days. The last dilution of the drug in a nutrient medium, from which it was not possible to obtain viable cells of the tested microorganisms, was considered bactericidal.

Studies were repeated 2 to 4 times.

The following types of microorganisms were used to determine the antimicrobial activity of disinfectants:

E. coli (E. eo ATCC No. 25922) as one of the most resistant species of the intestinal group of bacteria;

Staphylococcus aureus (§.aigei8 ATCC No. 6538r) as one of the most resistant species of coccal group of microorganisms;

representatives of the genus VaeShik (V. eeegeik No. 8035 ISTS) as spore-forming bacteria; culture of yeast-like and mold fungi: SaiShya a1Ysaik, AkregdShik shdeg VKM R-1 119 wai

PedNo.

Table 6

Minimum inhibitory drug concentrations (mg / ml)

Treatment Test cultures E. SOI ATCC Ha 25922 U. Aigueiz ATCC Ha b538r V. segeiz Ha 8035 even Satinla a1ysapz A. ηίββι VKM P1119 wai T1eyaega The composition according to the patent RF X® 2208048 0.20 0.3 0.35 0.5 1,0 The composition according to the patent RF Ha 2297248 0.22 0.35 0.4 0.55 1,2 Hydroxyethylidene diphosphonic acid 25 thirty thirty fifty 60 Ammonium Polydiallyldimethyl chloride 10 5 7 fifteen 25 The claimed composition: Composition 1 0,03 0.05 0.08 0.20 0.4 Composition 2 0,07 0.1 0.12 0.23 0.48 Composition 3 0.08 0.13 0.15 0.27 0.55 Composition 4 0.14 0.2 0.2 0.4 0.8 Composition 5 0.05 0.12 0.09 0.17 0.32

From the table it follows that the claimed composition inhibits the growth of pathogenic flora at much lower concentrations of active substances and, accordingly, are more effective.

So, from the table. Figure 6 shows that compositions containing three of the four claimed components, i.e. compositions according to the patents of the Russian Federation No. 2208048 and 2297248, have insufficient effectiveness of surface disinfection.

Moreover, the fourth component that is absent in each of these compositions, but present in the composition according to the present invention (in this case, hydroxyethylidene diphosphonic acid and polydiallyldimethylammonium chloride, respectively), taken separately, does not provide the best effect.

At the same time, a composition containing all four components unexpectedly provides surface decontamination efficiency with frost resistance, which was not achieved when using the composition of the three components, or when using the fourth component separately.

Thus, it is not obvious to a person skilled in the art that a composition containing all four components will work significantly better than a composition of three components or a fourth component taken separately.

These examples confirm the possibility of obtaining an unexpected technical result, which consists in enhancing the antiviral effect, enhancing flocculating properties and increasing frost resistance of working solutions, as well as improving biocidal properties.

- 11,069,555

Claims (16)

1. A disinfecting composition containing a water-soluble salt of alkyldimethylbenzylammonium; water soluble salt of polyhexamethylene guanidine; hydroxyethylidene diphosphonic acid or a water soluble salt thereof; a water-soluble salt of polydiallyldimethylammonium, the ratio of alkyl dimethylbenzylammonium salt: polyhexamethylene guanidine salt: hydroxyethylidene diphosphonic acid or its salt: polydiallyldimethylammonium salt in said composition is about 1: (0.3-30): (0.02-1): (0.02-1) by weight. 2. The disinfecting composition according to claim 1, characterized in that the alkyl dimethylbenzylammonium salt is an alkyl dimethylbenzylammonium chloride. 3. The disinfecting composition according to any one of claims 1 or 2, characterized in that the polyhexamethylene guanidine salt is polyhexamethylene guanidine hydrochloride. 4. The disinfecting composition according to any one of claims 1 to 3, characterized in that the hydroxyethylidene diphosphonic acid salt is the sodium salt of hydroxyethylidene diphosphonic acid. 5. The disinfecting composition according to any one of claims 1 to 4, characterized in that the polydiallyldimethylammonium salt is polydiallyldimethylammonium chloride. 6. The disinfecting composition according to any one of claims 1 to 5, characterized in that the ratio is salt of alkyldimethylbenzylammonium: salt of polyhexamethylene guanidine: hydroxyethylidene diphosphonic acid or its salt: salt of polydiallyldimethylammonium in the composition is selected from the group consisting of ratios of about 1: (0.3-3 ) :( 0.02-2) :( 0.01-1), 1: (0.3-3) :( 0.02-0.2) :( 0.01-1), 1: (0 , 3-3) :( 0.02-0.2) :( 0.01-0.1), 1: (3-30) :( 0.02-2) :( 0.01-1), 1: (3-30) :( 0.2-2) :( 0.01-1), 1: (3-30) :( 0.02-2) :( 0.1-1), 1: (3-30) :( 0.2-2) :( 0.1-1), 1: (0.3-3) :( 0.2-2) :( 0.01-0.1) and 1: (0.3-3) :( 0.2-2) :( 0.1 -1) by weight. 7. The disinfecting composition according to any one of claims 1 to 6, characterized in that the alkyl dimethylbenzylammonium salt is contained in an amount of about 1-10 wt.% Of the total weight of the composition. 8. The disinfecting composition according to any one of claims 1 to 7, characterized in that the salt of polyhexamethylene guanidine is contained in an amount of about 3-30 wt.% Of the total weight of the composition. 9. The disinfecting composition according to any one of claims 1 to 8, characterized in that the hydroxyethylidene diphosphonic acid or its salt is contained in an amount of about 0.2-2 wt.% Of the total weight of the composition. 10. The disinfecting composition according to any one of claims 1 to 9, characterized in that the polydiallyldimethylammonium salt is contained in an amount of about 0.1-1 wt.% Of the total weight of the composition. 11. A disinfecting composition containing alkyldimethylbenzylammonium chloride 1-10 wt.%; polyhexamethylene guanidine hydrochloride 3-30 wt.%; hydroxyethylidene diphosphonic acid sodium salt 0.2-2 wt.%; polydiallyldimethylammonium chloride 0.1-1 wt.%; water up to 100 wt.%. 12. The use of the composition according to any one of claims 1 to 11 as a flocculant for water purification. 13. The use of a composition according to any one of claims 1 to 11 for disinfection. 14. The application of item 13, wherein the disinfection is a disinfection of surfaces, equipment or ventilation and air conditioning systems. 15. A method of disinfecting water, comprising adding to the water a composition according to any one of claims 1 to 11. 16. A method of disinfecting surfaces, equipment or ventilation and air conditioning systems, including the use of a composition according to any one of claims 1 to 11.