RU2623061C1 - Means for skin diseases treatment have anti-inflammatory, wound-healing, antibacterial, anti-viral, anti-fungal action - Google Patents

Abstract

FIELD: pharmacology.

SUBSTANCE: invention relates to a skin treatment agent having an anti-inflammatory, anti-bacterial, anti-viral, anti-fungal activity that is an oil extract of Gynura procumbens leaves obtained in a specific manner.

EFFECT: agent is effective for skin diseases treatment.

6 cl, 16 ex

Description

The invention relates to the pharmaceutical industry, namely to medicines (options) based on plant materials - Gynura Procumbens, for external use, intended for the treatment of skin diseases with anti-inflammatory, wound healing, antibacterial, antiviral, antifungal effects.

Currently, bacterial infections of the skin and subcutaneous fatty tissue account for over 30% of all skin diseases, fungal infections of the skin are detected in 2% of all patients seeking medical help (any), and in the structure of inflammatory skin diseases, at different age periods, found in 68-93% of the population.

Of the greatest importance in the treatment of all of the above diseases are antibacterial, antifungal, antiviral, anti-inflammatory and wound healing topical preparations.

The wound process is complex and multi-stage and the drugs used to treat wounds act on various mechanisms depending on the stage of the disease.

From the prior art, preparations for the treatment of skin diseases based on plant components are widely known.

So, a document is known [1], in which an ointment for the treatment of aphthae, wounds, burns is disclosed. The ointment for the treatment of aphthae, wounds, burns contains a 5% aqueous solution of rivanol, yarrow extract, eleutherococcus extract, 1% aqueous solution of honsuride, 0.5% aqueous solution of levamisole, tannin, 10% liniment of dibunol taken in a certain ratio. This composition does not have a sufficiently high pharmacological activity for the treatment of aphthae, wounds, burns, does not have antifungal activity.

An ointment for external use with biostimulating properties for the treatment of burns, skin diseases and wound healing, which can also be used in cosmetology, is known from document [2]. The ointment for the treatment of burns, skin diseases and wound healing contains gum, beeswax, sea buckthorn oil, cow oil, pork fat and a biologically active substance selected from the group: aloe extract, plantain extract, succession extract, rosehip extract, hypericum oil, linseed oil , marigold oil, burdock oil, chamomile oil or propolis. The use of the ointment showed its stimulating effect on the regenerative processes of connective tissue and epithelium, which indicates the biostimulating effect of the ointment and is confirmed by a reduction in the healing time of wounds with good fixation and even distribution on the skin surface and ease of use. However, the lack of ointment is a multicomponent composition and insufficiently high pharmacological effect.

According to the document [3], a tool is proposed for nutrition and skin protection, for the treatment of burns, eczema, damaged tissues, and can also be used in veterinary medicine. The cosmetic product contains carbon dioxide, or alcohol, or water extract from the fruit bodies of the medicinal tinder drug and petroleum jelly. The cosmetic product has anti-inflammatory, wound healing and bactericidal action, but lower in comparison with other drugs. In addition, there is no antifungal effect.

From the document [4], an ointment is known which is used in the treatment of both superficial and deeply penetrating skin burns, as well as other skin diseases. The composition of the ointment in weight percent: 45 ... 67% edible sunflower oil, 15 ... 37% edible olive oil, 1 ... 2% extract of the plant Calendulae officinalis, 0.8 ... 1.8% incense, 2.5 ... 8% rosin, 6 , 5 ... 11.5% of white beeswax, 0.7 ... 1.8% of the main gallic acid bismuth and 0.7 ... 1.8% of camphor. The ointment is very effective for the quick and successful treatment of burns of the III and IV degrees. It is also used to treat lower leg ulcers, as well as in gynecology for the treatment of cervical inflammation. At the same time, this ointment does not have antifungal activity.

An ointment based on natural components and having anti-inflammatory and wound healing effects is known from document [5]. Ointment in addition to natural vegetable oils may contain bear fat, bear bile, propolis, lanolin, honey, mummy. The ointment can be used to treat hemorrhoids, burns, sinusitis, post-stroke conditions, gunshot and stab wounds, trophic ulcers, postoperative fistula, inflammation of the appendages, diabetic microangiopathy of the lower extremities, as well as papillomas, birthmarks, seborrhea and psoriasis. This composition has a low antibacterial activity and does not have antifungal activity.

The authors of the present invention developed compositions based on plant materials - leaves of Gynura Procumbens.

Gynura Procumbens from the family of asters was born many centuries ago on the African continent, then the plant successfully migrated to Asia, where it was appreciated by the healers of China and Southeast Asia. It was used simply as food and at the same time as a cure for disease. In Southeast Asia, Ginura is part of a balanced diet and healthy lifestyle. Phytochemical studies confirm that the extract from Ginura leaves is an excellent natural source of many potent biologically active components, including flavonoids, carotenoids, alkaloids and essential oils. An active effect on metabolic processes puts Ginuru in a number of other medicinal plants, which are actively used as primary protection for cancer patients before chemotherapy and radiotherapy. The results of clinical studies confirm the therapeutic effect of Ginura in diabetes, kidney disease, gastroenterological and cardiovascular diseases. The plant has pronounced antioxidant properties, inhibits the growth of cancer cells in the body, has an anti-inflammatory effect and helps to increase reproductive function.

A document [6] discloses the use of Gynura Procumbens alcohol extract for the treatment of various skin diseases. In particular, the anti-inflammatory, antioxidant effect of the Gynura Procumbens alcohol extract is disclosed.

From the document [7] it is known that Gynura Procumbens is widely used in medicine for the treatment of inflammation, rheumatism, and viral diseases. Gynura Procumbens alcohol extract was tested for anti-inflammatory activity, which was fractionated in water and ethyl acetate. Gynura Procumbens aqueous extract has been shown to have no anti-inflammatory effect, while organic extraction exhibits pronounced anti-inflammatory activity.

The paper [8] studied the antioxidant activity of Gynura Procumbens. Gynura Procumbens methanol extract was fractionated in chloroform, ethyl acetate, butanol, water. Among all the extracts, the ethyl acetate fraction showed a more pronounced antioxidant activity.

From the document [9], the antiproliferative effect of alcohol extraction of Gynura Procumbens leaves is known.

In the document [10], the chemoprophylactic effect of Gynura Procumbens extract was investigated. The results showed that Gynura Procumbens has antiproliferative, antioxidant properties.

The patent [11] discloses a composition for the prophylaxis and treatment of inflammatory and allergic disorders, containing as an active component an extract (selected from aqueous, methanol, ethanol and / or butanol) Gynura Procumbens.

The prototype of the proposed invention is a document [12], which presents a report on the study of Gynura Procumbens and funds based on it. This document discloses that Gynura Procumbens has great therapeutic potential, a variety of pharmacological activities: antihypertensive, cardioprotective, antihyperglycemic, anti-cancer, antibacterial, antioxidant, general protective, anti-inflammatory. Gynura Procumbens is characterized by a high content of biologically active substances: flavonoids, glycosides, etc. This document describes the use of the aqueous and alcoholic extract of Gynura Procumbens leaves. The disadvantage of the products from Gynura Procumbens described in this document is that the products have less pronounced pharmacological activity for treating the skin, are characterized by a low content of biologically active substances. The result is insufficient clinical results in the treatment of skin diseases.

The objective of the proposed invention was to develop a tool based on a unique plant Gynura Procumbens, the use of which will lead to high clinical results in the treatment of various skin diseases due to its high anti-inflammatory, antibacterial, antiviral, wound healing, antifungal effects.

The technical result consists in the fact that the proposed tool, which is an oil extract of Gynura Procumbens leaves, is highly effective in treating skin diseases due to its high anti-inflammatory, antibacterial, antiviral, wound healing, antifungal effects.

The technical result is achieved by the fact that the proposed tool for the treatment of skin diseases has anti-inflammatory, antibacterial, antiviral, wound healing, antifungal effects, is an oil extract from the leaves of Gynura procumbens, obtained by extraction of purified fresh leaves of Gynura procumbens with vegetable oil, taken in in a ratio of 1: 5-7, at a temperature of 80 ° C for 8 hours, followed by filtration, or by extraction of the dried leaves of Gynura procumbens with vegetable oil, taken in a ratio of 1: 35-50, at a temperature of 80 ° C for 8 hours, followed by filtration.

To obtain the proposed funds, you can use any vegetable oil, in particular grape seed oil, rapeseed, soybean, sunflower.

The use of products obtained by oil extraction allows one to obtain high clinical results: quick resolution of the inflammatory process, suppression of viruses, rapid reduction of bacterial contamination, effective healing of wounds, fibrinolytic property, acceleration of epithelization and maturation of granulation tissue, removal of edema, hyperemia, quick restoration of the skin, elimination mycoses, normalization of the sebaceous glands, a decrease in pore size of the skin.

In the course of clinical studies, it was found that the optimal ratio of feedstock and vegetable oil is: for fresh leaves 1: 5-7, for dry leaves 1: 35-50, respectively.

The optimal extraction conditions are - temperature 80 ° C for 8 hours.

When using other ratios and modes of extraction, the content of biologically active substances in the final product was reduced.

The proposed products can be effectively used to treat skin diseases such as allergic dermatitis, contact dermatitis, atopic dermatitis, eczema, psoriasis, bacterial infections, viral dermatoses, such as warts, papillomas, herpes simplex, skin mycoses, insect bites.

The method of preparation of funds.

Example 1

Fresh leaves of Gynura procumbens were cleaned by washing in running water, then immersed in an ethyl alcohol solution of at least 70% for 5 minutes.

After separation from the disinfectant, the cleaned fresh leaves of Gynura procumbens were immersed in a container and poured with grape seed oil in a ratio of 1: 5 (150 g of leaves and 750 ml of water) and kept at a temperature of 80 ° C for 8 hours. Then the oil extract was separated from the leaves by filtration. The resulting oil extract is poured into sealed glass vessels and stored in a dark, dry room at a temperature of 10-28 ° C.

Example 2

Fresh leaves of Gynura procumbens were cleaned by washing in running water, then immersed in an ethyl alcohol solution of at least 70% for 5 minutes.

After separation from the disinfectant, the cleaned fresh leaves of Gynura procumbens were immersed in a container and poured with soybean oil in a ratio of 1: 6 (150 g of leaves and 900 ml of water) and kept at a temperature of 80 ° C for 8 hours. Then the oil extract was separated from the leaves by filtration. The resulting oil extract is poured into sealed glass vessels and stored in a dark, dry room at a temperature of 10-28 ° C.

Example 3

Fresh leaves of Gynura procumbens were peeled. The purified fresh leaves of Gynura procumbens were immersed in a container and filled with rapeseed oil in a ratio of 1: 7 (150 g of leaves and 1050 ml of water) and kept at a temperature of 80 ° C for 8 hours. Then the oil extract was separated from the leaves by filtration. The resulting oil extract is poured into sealed glass vessels and stored in a dark, dry room at a temperature of 10-28 ° C.

Example 4

The dried leaves of Gynura procumbens were immersed in a container and filled with grape seed oil at a ratio of 1:35 (20 g of leaves and 700 ml of water) and kept at a temperature of 80 ° C for 8 hours. Then the oil extract was separated from the leaves by filtration. The resulting oil extract is poured into sealed glass vessels and stored in a dark, dry room at a temperature of 10-28 ° C.

Example 5

The dried leaves of Gynura procumbens were immersed in a container and poured with sunflower oil in a ratio of 1:43 (20 g of leaves and 860 ml of water) and kept at a temperature of 80 ° C for 8 hours. Then the oil extract was separated from the leaves by filtration. The resulting oil extract is poured into sealed glass vessels and stored in a dark, dry room at a temperature of 10-28 ° C.

Example 6

The dried leaves of Gynura procumbens were immersed in a container and filled with rapeseed oil in a ratio of 1:50 (20 g of leaves and 1000 ml of water) and kept at a temperature of 80 ° C for 8 hours. Then the oil extract was separated from the leaves by filtration. The resulting oil extract is poured into sealed glass vessels and stored in a dark, dry room at a temperature of 10-28 ° C.

In all cases, the oil was obtained in the form of a clear liquid from greenish yellow to dark green (depending on the base oil) with a grassy odor.

A study was carried out of the obtained oil extracts for the content of biologically active substances in them by the method of spectral-dynamic analysis and mass spectrometry.

The resulting oil extracts from the leaves of Gynura procumbens are characterized by a high content of chlorophyll, trace elements, and amino acids: proline, leucine, aspartic acid, pheninalanine, tryptophan, histidine, isoleucine, arginine, taurine, glutamic acid, lipoic acid, malic acid, organic phytohormones that have a wide range of pharmacological activity.

The following are examples of a clinical trial of the proposed oil extracts of Gynura procumbens leaves and a comparison of their pharmacological activity compared to known agents.

Example 7

A study of the pharmacological activity of the proposed oil extracts from the leaves of Gynura procumbens was carried out on a burn wound model [13].

To do this, under ketamine-droperidol-atropine anesthesia in Wistar rats weighing 220-250 g, a thermal burn was reproduced in the back region (the depilated surface was exposed to a 300 W halogen lamp at a distance of 1 cm for 10 s). The experiments were carried out in accordance with the standards for the humane treatment of animals, which are regulated by the "Rules for the Use of Experimental Animals" [14] in accordance with the protocol approved by the Committee on Biomedical Ethics of the NRCRC SB RAMS.

The animals were divided into an experimental group and a control group.

The animals of the experimental groups immediately after manipulation and then throughout the experiment were applied the studied oil extracts obtained in examples 1-6, and the rats of the control group were applied funds that are aqueous or alcohol or ethyl acetate extract of Gynura procumbens leaves.

Excretion of animals from the experiment was carried out in terms: 3, 7 and 14 days.

For histological evaluation of pharmacological activity, burn wound fragments were fixed with FineFix solution. The obtained material was poured into paraffin, sections were prepared with a thickness of 5 μm, which were stained according to the Van Gieson method [15].

It was shown that when using the proposed means (according to examples 1-6), the area of the burn wound decreased by 55% on the 3rd day, while extracts from the experimental group showed a decrease in area to only 25-30% on the 3rd day. On the 7th day, the area of the burn wound in the experimental group decreased by 74%, on the 14th day the wounds completely healed.

Also, in the experimental groups, the content of gram (-) bacteria (CFU / g) and gram (+) bacteria (CFU / g) decreased by 76% already by 3 days, while in the control group the number of bacteria decreased by 3 days only 32-41%.

Example 8

Wound healing activity.

The wound healing activity of the studied oil extracts (according to examples 1-6) was evaluated planimetrically according to A.V. Cherkasov [16]. So, by 3 days of the experiment, the burn area decreased by 2-2.5 times in all groups of animals compared with the initial value (4 cm ² ).

By the end of the experiment (14 days) in the experimental groups, almost complete healing of the thermal burn was observed - from 0.5 to 1.0% of the initial burn area was preserved compared to 22.3% in the control group.

Evaluation of the dynamics of the processes of epithelization of a wound defect consisting in the formation of an epithelium at the site of skin damage is an important characteristic of the rate of regeneration.

It was found that when using the studied compounds (according to examples 1-6), complete epithelization of the burn wound was achieved by 14 days of the experiment in the experimental groups. At the same time, epithelization in the control group does not occur by 14 days of the experiment. On histological sections, maturation of granulation tissue in a burn wound without epithelization is observed.

Thus, as a result of the studies, a pronounced wound healing activity of the studied compounds was revealed, which manifests itself in the early epithelization of the burn wound compared to the control group of animals.

Example 9

Anti-inflammatory activity.

When assessing the anti-inflammatory activity of the studied compounds (according to examples 1-6), it was shown that in the control group of animals the healing process of the burn wound morphologically corresponds to the canons of the development of the inflammatory response, while even at 3 days the peak of the severity of neutrophilic leukocyte infiltration in the form of a wide band across all layers of the wound with swelling of adjacent tissues.

The use of the studied compounds (according to examples 1-6) by 3 days of the experiment significantly reduced edema and the severity of neutrophilic infiltration - the exudative phase of inflammation in the wound area. Moreover, the studied compositions (according to examples 1-6) contribute to a decrease in the area of necrosis and a decrease in edema in the tissues of the burn wound.

Example 10

Antibacterial activity.

The study of the antibacterial activity of the proposed compositions (according to examples 1-6) was carried out by diffusion in agar on solid nutrient media by analyzing zones of growth inhibition of test microorganisms used to determine the antimicrobial effect of drugs. Studies were carried out for the following standard strains of microorganisms: Staphylococcus aureus ATCC 653 8-P, Staphylococcus aureus 209-P, Escherich-ia coli ATCC 25922, Bacillus subtillis ATCC 6633, Candida albicans ATCC 865-653. Pseudomonas aeruginosa ATCC 27853, Proteus vulgaris. It was established that a pronounced antimicrobial effect is actively manifested in relation to all of the above test cultures.

The local anesthetic activity of the developed compounds was studied in vivo by the Rainier-Valet method, which is based on the change in the sensitivity of the rabbit’s cornea when a test sample is applied to it, expressed in response to an irritant in the form of a closure of the eyelid.

0.1 g of the test compounds were injected into the conjunctival sac of the rabbit's eye using a syringe. Using calibrated hairs, rhythmic irritations were applied to the cornea at a frequency of 100 beats per minute after 1, 2, 5, 8, 10, 12, 15 minutes and then every 5 minutes until the eyelids closed. The time between the introduction of a sample of the composition (according to examples 1-6) and the onset of the manifestation of a pharmacological action was taken as the rate of onset of local anesthesia. The relative strength of the anesthetic effect was calculated according to the Rainier index, summing up the amount of irritation in 13 measurements, starting from the 8th minute, within an hour.

The indicator of complete anesthesia was considered the absence of a blinking reflex for 1 min at 100 contacts of calibrated hairs with the cornea of the eye.

The duration of general anesthesia was characterized by the time from the start of anesthesia to the complete restoration of sensitivity. The results obtained were processed by the method of variation statistics [17]. The development time of the anesthetic effect is 1-5 minutes, the time of complete anesthesia is 55-70 minutes, the Rainier index is 1100-1400 conv. units, recovery time sensitivity 70-80 minutes.

High values of the Rainier index, the duration of general anesthesia for the proposed pharmacological formulations (compared with drugs from the control group) indicate not only the increased antibacterial effect of drugs from the experimental group, but also the prolongation of the anesthetic effect.

Example 11

Antifungal activity.

In three groups of guinea pigs of 8 animals, each was plucked in the caudal region of the back in a 4 × 4 cm area, after which a dense standard suspension of a virulent laboratory strain of the fungus, gypsum trichophyton, was applied to the exposed area.

The test results were as follows. In the first group untreated, by the tenth day from the moment of infection, all the signs of experimental mycosis (peeling, hyperemia, infiltrate, crust formation) developed. In the second group of pigs (control) treated with Gynura Procumbens aqueous or alcoholic or ethyl acetate extract, by the tenth day from two to three animals only peeling was observed and from five to six peeling with mild hyperemia (depending on the extract used). In the third group of pigs treated with the drug (according to examples 1-6), three animals had smooth skin without all signs of mycosis by the 8th day. Two pigs have smooth skin without signs of mycosis - by the 10th day, three pigs - peeling, but without hyperemia by the tenth day. In no case of the use of the claimed drug (according to examples 1-6) were there any signs of skin-irritating effects.

Thus, the proposed compositions obtained in examples 1-6, allowed to reduce the treatment time for patients with fungal skin diseases.

Example 12. Antiviral activity.

Patient S., 27 years old. Ds: Multiple warts. Concomitant diseases have not been identified.

The tool obtained in example 3 was applied topically to the wart from 1 to 5 times a day, preferably from 1 to 2 times a day. After application, the product was rubbed and allowed to be absorbed. This treatment was repeated until the warts at least visually disappear.

The treatment indicates a positive treatment, removal of warts. Removal occurs in a short time, after the procedure, the skin is restored, without visible pigmentation at the site of the wart, as well as the absence of a scar.

The use of means of the control group showed low antiviral activity.

The tool obtained in examples 1-6, also showed high efficiency in the treatment of papillomatosis.

The proposed funds obtained in examples 1-6 were studied in 98 patients with various skin diseases: namely contact dermatitis, allergic dermatitis, atopic dermatitis, eczema, psoriasis, seborrheic dermatitis, perioral dermatitis, prickly heat, bacterial infections, viral dermatoses, such like warts, herpes simplex, papillomas, mycoses of the skin and mucous membranes, insect bites.

The following are examples of the use of the proposed funds obtained in examples 1-6, for the treatment of some of these diseases.

Example 13

Patient O., 23 years old

Ds: Atopic dermatitis, moderate severity, exacerbation phase. Concomitant diseases have not been identified.

From the anamnesis it is known that blood pressure suffers from birth, in connection with artificial feeding. I contacted a dermatologist at my place of residence, treatment was prescribed: antihistamines, topical glucocorticoids with a temporary positive effect. Marks an exacerbation of the skin process in the autumn-winter time, after contact with household chemicals. Last exacerbation after contact with phosphate detergent. On examination, rashes are localized on the palmar surfaces, knee, elbow bends, are represented by multiple papules, fissures, lichenification, subjectively pronounced itching. SCORAD index 7.0 points.

The patient was prescribed a new external agent (obtained according to example 6) 2 times a day to apply in the area of rashes. The use of a new external agent was carried out for 14 days, after which there was a remission. The patient noted the excellent tolerance of the product, was satisfied with the properties of the skin after treatment, since dryness and tightness of the skin were completely absent; its use is continued prophylactically after each water treatment; clinical remission of blood pressure remains.

The proposed external agent (obtained according to examples 1-6) was used to treat patients with mild atopic dermatitis (n = 15), while the standardized SCORAD disease severity index before treatment was 8.1 ± 1.3 points. The comparison group (control group) consisted of patients with mild atopic dermatitis, before the start of therapy, the average group index SCORAD was 8.3 ± 0.9 points, that is, it did not statistically differ from the index of the main group. After the completion of the 4-week course of therapy in both groups, the residual symptoms of atopic dermatitis (BP) were evaluated by the attending physician and the subjective attitude of the patients to the external treatment (therapy compliance factors) was studied.

Comparative studies revealed a significant superiority of the new external agent obtained according to examples 1-6, when using it in patients with blood pressure 1.5 times less than when treating drugs from the control group, peeling of the skin was noted, 2 times less residual dry skin was recorded and its redness, skin tightening was observed about 4 times less often, itching, burning and swelling of the skin were completely absent.

According to patients, the new external agent was characterized by excellent and good tolerance (93.3%), high satisfaction with the use of the drug and overall satisfaction of the patient with skin properties after treatment (100% and 93.3%, respectively).

Example 14

Patient M., 35 years old.

Ds: Vulgar psoriasis, progressive stage.

Concomitant diseases: metabolic syndrome.

From the anamnesis it is known that he considers himself sick for 10 years, when, after suffering stress, he noted the appearance of rashes in the elbow joints. I did not go to doctors, I was not treated on my own. In 2016, he noted an exacerbation of the skin process, for no apparent reason. On examination, the skin process of a chronic inflammatory nature is localized on the elbow joints, represented by single papules, plaques up to 5 cm in diameter, with severe hyperkeratosis, without subjective sensations. In this connection, treatment was prescribed as an external therapy according to example 1, the agent should be applied after water procedures and during the day. As a result of the treatment, it was noted: on day 3, a decrease in rashes by 30%, on day 10, the resolution of rashes, on the site of plaques, foci of post-inflammatory hyperpigmentation. There are no new rashes.

Example 15

On examination and treatment, 20 patients with various forms of eczema were observed. Assigned funds obtained in examples 1-6, which are smeared on the affected areas 2 times a day. The group included patients with chronic eczema.

Patients noted good tolerance to the drug. Side effects and complications were not identified. Analysis of the results of the study showed that, against the background of therapy, all patients recovered. As a rule, itching decreased immediately after application of the drug, and epithelialization of foci and resolution of rashes was noted by the end of the second week.

Example 16

Patient P., 37 years old, complained of vesicular eruptions that appear on normal or slightly reddened edematous skin of the genital organs and perianal region, preceded by eruptions of itching, burning, tingling, pain in the inguinal-scrotal area. After 6 days, the bubbles burst, forming surface erosion. From the medical history of the disease, it is known that it suffers from genital herpes for about 5 years, relapses 2-3 times a year. The diagnosis is confirmed by the results of immunological blood tests by determining antibodies to the herpes type II antigen (HSV-2) by the enzyme immunoassay. In the study of the immune status: a reduced number of T-lymphocytes (0.92⋅409 / L), T-helpers (0.37⋅109 / L), a decrease in the Tx / Tc index (3.08), an increased content of B-lymphocytes ( 0.35⋅109 / l), IgG (9.5 g / l), reduced IgM (0.78 g / l) and IgA (0.88 g / l), as well as sIgA in the urethral discharge - 0.28 g / l (indicators were compared with the results of studies of healthy donors).

The patient underwent a course of treatment with the agent obtained in example 4, which was applied 2 times a day in the morning and evening at the rash site for 3 weeks.

After the first week of the course, the patient noted the complete disappearance of pain, burning, itching. By the end of the course of treatment, lymphadenopathy disappeared, and a clear regression of herpetic eruptions was observed. When studying the immune status: the number of T-lymphocytes (1.22⋅10%), T-helpers (0.55⋅10%) increased, the Tx / Tc index (4.33) increased, and the number of B-lymphocytes decreased (0, 28⋅109 L), IgG (8.81 g / L), increased levels of IgM (0.82 g / L) and IgA (1.18 g / L), as well as sIgA (0.42 mg / ml), that is, these indicators are much closer to the level of healthy individuals.

Thus, the proposed tool has an antiviral effect, gives a high therapeutic effect.

The treatment with the proposed agents has shown high efficacy, rapid removal of inflammation, swelling, suppression of viruses, rapid reduction of bacterial contamination, elimination of mycoses, effective healing of wounds, cleansing of wounds from necrotic plaque, acceleration of epithelization and maturation of granulation tissue, effective restoration of the skin, rapid removal of subjective sensations of itching burning.

Thus, the proposed funds are effective for the treatment of various skin diseases with pronounced anti-inflammatory, wound healing, antibacterial, antiviral, antifungal effects.

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Claims (6)

1. An agent for the treatment of skin diseases that has anti-inflammatory, wound healing, antibacterial, antiviral, antifungal action, which is an oil extract from Gynura procumbens leaves, obtained by extracting purified fresh Gynura procumbens leaves with vegetable oil, taken in a ratio of 1: 5-7, with temperature of 80 ° C for 8 hours, followed by filtration. 2. An agent for treating skin diseases that has anti-inflammatory, wound healing, antibacterial, antiviral, antifungal effects, which is an oil extract from Gynura procumbens leaves, obtained by extracting dried Gynura procumbens leaves with vegetable oil, taken in a ratio of 1: 35-50, at a temperature 80 ° C for 8 hours followed by filtration. 3. The tool according to claim 1, characterized in that the skin diseases are selected from the group: allergic dermatitis, contact dermatitis, atopic dermatitis, eczema, psoriasis, bacterial infections, viral dermatoses, such as warts, papillomas, herpes simplex, skin mycoses, bites insects. 4. The tool according to claim 1, characterized in that the vegetable oil is selected from the group: grape seed oil, rapeseed oil, soybean oil, sunflower oil. 5. The tool according to claim 2, characterized in that the skin diseases are selected from the group: allergic dermatitis, contact dermatitis, atopic dermatitis, eczema, psoriasis, bacterial infections, viral dermatoses, such as warts, papillomas, herpes simplex, skin mycoses, bites insects. 6. The tool according to p. 2, characterized in that the vegetable oil is selected from the group: grape seed oil, rapeseed oil, soybean oil, sunflower oil.