RU2546878C2 - Method for increasing reproductive ability of seed bulls - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention refers to veterinary medicine. A method provides feeding the bulls with stimulating mineral feed additive daily. An aqueous solution of the preparation Alexanate, micellate of calcium carbonate 300 g per a head for three months; before administering, the preparation is dissolved in water in ratio 1:50.

EFFECT: using the method enables higher amount and quality of sperm production by breeding seed bulls for artificial insemination.

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Description

The invention relates to veterinary medicine, in particular animal husbandry, and more specifically to methods and means that increase the quantity and quality of sperm products obtained from breeding bulls for artificial insemination of cows.

It is known the use of medicinal plants biostimulants to increase the sexual activity of male animals. It is known that feeding 400 grams of safflower-like Levzea grass meal (maral root) significantly increases the sexual activity of bulls and boars (see Biostimulant for animals // Host. No. 10, 1991, p.40).

Feeding 2-3 g of the powder of the roots of the pink radiola (golden root) to the boars for 60 days in the spring-summer period, allows to increase the ejaculant yield by 2.0-2.2 times in comparison with the control. The number of received sperm doses per producer increased by 14.2-28.6% in the summer and by 13.3-26.7% in the autumn (see Jamaldinov A. Narizhny A. Use of herbal preparations to increase the potency of boars / / Pig production, No. 2, 2004, p.20-23).

With good results on the use of plant biostimulants for manufacturing bulls, the disadvantages of such preparations is that in nature such plants biostimulants are listed in the Red Book and their collection is limited, and they are not yet introduced into the culture on an industrial scale, which limits their use in animal husbandry.

It is known to use vitamins A, E and krezocin as agents that increase sperm production in bulls (see Khristianovsky P.I. Physiological reaction and reproductive ability of bulls of Kazakh white-headed breed when vitamins A, E and cresociation are included in the diet. Abstract of Cand. Diss. Zhodino , 1983, 16 pp.).

With the ease of use and availability of drugs, the disadvantage of these agents is their low efficiency when used against various stresses on the animal (intensive use, diagnostic and veterinary treatments, etc.).

It is also known to use a preparation (β-phenyl- - amino-butyric acid hydrochloride) as a means for increasing the sperm production of male farm animals. Phenibut is administered to bulls in addition to the diet at a dose of 1 mg per 1 kg of body weight. The drug was fed in a mixture with food according to the scheme: once a day for 15 days. The course was repeated twice with a 7-day break.

It was found that with the use of phenibut, the ejaculant volume increases by 4.8%, the sperm concentration - by 16.5%, and their number in the ejaculant - by 22.0%.

This tool allows you to increase sperm production of producers by 8-40% compared with the control on the background of exposure to various stressors (see. "Means for increasing sperm production of male farm animals." A.S. No. 1422589, A61K 31/00. Publ. 30.09.88 Bull. No. 36).

With good results, the application, the limitation of the use of the drug phenibut in animal husbandry is that it is a pharmacopeia, medical, prescription drug, which inhibits its use in agricultural production.

The objective of the invention is to develop a method of increasing the reproductive abilities of bulls of producers without the use of expensive medications, but using only domestic products from natural mineral natural raw materials that do not require large labor costs and are simple to implement, but can significantly increase the quantity and quality obtained from bull seed production.

The problem is solved in that as a stimulating mineral feed supplement, the bulls are daily fed an aqueous solution of the calcium carbonate micellate “Aleksanat Zoo”, and the drug is administered at a dose of 300 g per head for three months, while the drug is diluted with water in a ratio of 1 before administration: fifty.

"Aleksanat Zoo" is a new domestic drug developed using the latest technology, which contains mineral elements in the form of micelles, which significantly improves their absorption and provides the emergence of new properties.

Micellates are obtained from environmentally friendly natural carbonate minerals by deep peptization using selective cavitation in resonance mode on supersaturated sorbed shells of aggregate systems in the mother liquor. The size of the resulting particles is 10 ^-6 -10 ^-7 m, the active surface is up to 500 m ² / ml, the density is up to 1.6 g / ml. As a result of the high non-stoichiometric composition, the resulting preparations exhibit abnormally high adhesion, directional diffusion, autolysis, catalytic and other physicochemical properties. When the Aleksanat Zoo preparation is introduced into water, each particle of the mineral coated with numerous water layers in the form of a neutrally charged micelle, due to its high zeta potential, acquires high mobility and great activity. It is able to react with extraneous impurities in aqueous solutions, and when it enters the body of animals and birds, it delivers the necessary macro- and microelements to those organs and systems where they are needed.

Calcium carbonate micellate “Alexanat Zoo” as a mineral feed additive is produced according to TU 5743-056-54651030-2009, dated November 23, 2009

Certificate of Conformity No. ROSS RU.PN34.B00302 dated 11/23/2009. Manufacturer CJSC Petrokhim, Belgorod. According to organoleptic indicators, the additive meets the requirements specified in table 1.

Table 1 Characteristic of the drug "Aleksanat Zoo" Name of indicator Characteristics Appearance, color White liquid Smell Without smell Density, g / l 1400-1550

The content of mineral elements of the drug "Aleksanat Zoo" meets the requirements specified in table 2.

table 2 The content of mineral elements (g / l) in the drug "Aleksanat Zoo" Name of indicator Brand "O" Calcium 280-320 Magnesium 10-20 Iron 0.1-0.5 Zinc 1-2 Iodine 0.02-0.04 Silicon 1-2 Copper 0.2-0.4 * At the request of the customer, the drug can additionally be enriched with other trace elements.

Studies conducted at the Research Institute of Animal Husbandry and VNITIP poultry farming of the Russian Academy of Medical Sciences, showed that "Aleksanat Zoo" has a high biological activity and has a significant impact on the formation and development of the functional systems of the body, allows you to set a large starting energy for their growth, which determine productive health, resulting in more fully realized the genetic potential of their productivity, which can significantly improve the economic performance of production. The introduction of the drug into drinking water leads to the appearance of new properties in it, due to which metabolic intensification and a decrease in the incidence rate in farmed animals are achieved. Water containing calcium carbonate micellate neutralizes the effects of toxic substances and various contaminants due to the high adsorption capacity of the drug. The mineral composition of water is normalized by the vital key component - calcium, while the physicochemical properties of water change, which favorably affects the development of animals. Since it is shown that the best assimilation of various compounds occurs in micellar-colloidal form, the introduction of micellar and micronutrients lacking for the development of the organism in micellar form along with drinking water seems most appropriate. The multilateral effect of micellar species preparations on the animal organism allows obtaining a significant economic effect (reducing production costs and increasing profitability) while maintaining environmental safety and improving product quality.

Experimental studies of the influence of the Aleksanat Zoo watering of the preparation on the manufacturing bulls were performed at Uralplemcentr OJSC. The object of the study was bull breeding bulls of the Holstein breed of imported selection with an average live weight of 970 kg (890-1100 kg), the deep-frozen sperm of which is packaged in polypropylene straws (sequins), supplied and used for artificial insemination of cows and heifers in all agricultural organizations for the production of milk Ural region.

To evaluate the bulls by reproductive ability, we determined sexual activity, monitored the indicators of native sperm: the average volume of ejaculate (ml) and the concentration of sperm in the ejaculate (billion / ml) obtained in the doublet cage, the activity of sperm (in points), the number of ejaculates received and doses (polypropylene straws), sanitary and biological marriage (in% of frozen doses).

Qualitative and quantitative characteristics of freshly obtained and cryopreserved semen of breeding bulls were determined in accordance with technical requirements and test methods according to GOST: 23754-79, 26030-83.

In the process, they were guided by the “Instructions on the technology of organizations for artificial insemination and transplantation of farm animal embryos” (Order of the Ministry of Agriculture of the Russian Federation No. 819 of 2003).

The purpose of the scientific and production experience was to study the effect of drinking the drug "Alexanat Zoo" according to the proposed scheme on biochemical, including hormonal, and reproductive capacity of bulls. According to the results of andrological medical examination, two groups of full-age bulls-analogues of the French selection of 6 animals each were formed. Experimental animals in addition to the main diet received 300 g of the drug "Alexanat Zoo" daily for 3 months. Before use, the drug was diluted in a ratio of 1:50 with water. The control bulls received a diet adopted for this age and live weight.

In animals of the control and experimental groups, blood was taken for biochemical studies before use and every 30 days. Blood was taken from the caudal vein. The preparation of blood serum samples was carried out according to standard methods.

One month after the start of “Aleksanat Zoo” drinking and within three months after the end of drinking, the biological parameters of bull sperm productivity were taken into account: ejaculate volume, sperm concentration, activity, survival, number of sperm doses received.

Biochemical studies of blood serum were performed on a Chem Well Combi automatic biochemical analyzer from Awaveness Technology, USA using standard reagent kits from Vital Diagnostics Spb.

Serum levels of cortisol, testosterone, free thyroxin, and triiodothyronine were determined using an enzyme-linked immunosorbent assay kit: Multiscan EX spectrophotometer, WellWash collar, Titramax shaker-incubator, using HemaMedika kits (Moscow).

Sperm laboratory tests were carried out in the technological laboratory of Uralplemtsentr OJSC using the SQA-Vb bull sperm quality analyzer from Medical Electronic Systems Ltd (Israel).

The quantitative indicators obtained were mathematically processed on a Pentium PC using the Microsoft Excel statistical analysis package.

To study the effect of drinking Aleksanat Zoo on biochemical parameters before drinking mineral supplements, the experimental group was taken 30 and 60 days after the start of drinking on the bulls of both groups. The results of scientific and production experience at Uralplemtsentr OJSC showed that the use of the Aleksanat Zoo additive had a positive effect on the state of mineral metabolism in bulls. Laboratory studies found that after feeding the experimental group of bulls supplements, the serum calcium significantly increased: 30 days after drinking the drug by 18.8%, after 60 days - by 20.8%. There was also a decrease in the content of phosphorus, magnesium, an increase in the level of copper, zinc, iron (table 3).

Table 3 Effect of Aleksanat Zoo Drinking on Mineral Exchange of Bulls Indicator Initial data After 30 days After 60 days The control Experience The control Experience The control Experience Calcium, mmol / L 1.75 ± 0.11 1.78 ± 0.0 8 1.67 ± 0.12 1.81 ± 0.06 1.79 ± 0.15 2.15 ± 0.14 Phosphorus, mmol / L 2.28 ± 0.19 2.73 ± 0.15 2.24 ± 0.14 2.52 ± 0.12 2.32 ± 0.09 2.26 ± 0.06 Magnesium, mmol / L 1.29 ± 0.05 1.46 ± 0.07 1.32 ± 0.09 0.98 ± 0.04 1.27 ± 0.07 0.98 ± 0.03 Potassium, mmol / L 5.69 ± 0.41 6.34 ± 0.30 5.52 ± 0.36 3.58 ± 0.26 5.06 ± 0.27 4.16 ± 0.29 Sodium, mmol / L 159.2 ± 9.26 147.2 ± 10.85 161.4 ± 2.45 144.9 ± 2.45 167.1 ± 4.21 155.9 ± 3.17 Chlorides, mmol / L 104.6 ± 2.33 108.8 ± 3.51 107.1 ± 1.23 101.5 ± 1.31 103.9 ± 0.99 98.1 ± 0.84 Alkaline phosphatase, IU / L 237.1 ± 18.3 26.2 ± 23.1 197.9 ± 11.3 211.7 ± 12.7 204.00 ± 9.1 189.00 ± 12.6 Copper, mmol / L 21.19 ± 5.11 24.14 ± 4.13 19.07 ± 5.12 12.21 ± 1.24 11.24 ± 2.14 15.07 ± 1.17 Zinc, mmol / L 19.12 ± 2.16 20.48 ± 1.88 11.19 ± 0.92 13.94 ± 0.64 12.39 ± 0.51 14.52 ± 0.56 Iron, μmol / L 24.98 ± 1.49 29.26 ± 1.37 28.39 ± 1.27 29.81 ± 1.24 30.39 ± 2.11 27.81 ± 1.32

The positive effect of mineral supplementation on the thyroid hormones has been established. Despite the fact that 30 days after the use of Aleksanat Zoo in the experimental group there was a decrease in the content of free fractions of thyroid hormones in both groups, 60 days after the start of the experiment, the level of free triiodothyronine and free thyroxine in the blood serum of experimental animals increased by compared with the control 36.2 and 6.1%, respectively. The sum of free triiodothyronine and free thyroxine in the blood serum of bulls treated with the supplement increased over the entire period by 18.3% (32.87 ± 4.32 versus 38.84 ± 4.95 pmol / L). The ratio of thyroid hormones (CT3 / CT4) to day 60 in the experimental group increased by 0.21 (from 0.68 to 0.89) (table 4).

Table 4 Hormonal profile of bulls when using "Aleksanat Zoo" Indicator Initial data After 30 days After 60 days The control Experience The control Experience The control Experience ST3, pmol / l 13.51 ± 3.01 13.47 ± 2.31 8.03 ± 2.19 11.52 ± 1, 86 14.11 ± 0.84 18.34 ± 3.14 CT4, mmol / l 20.11 ± 0.58 19.35 ± 0.58 15.03 ± 0.64 17.12 ± 0.86 17.58 ± 0.79 20.53 ± 0.92 ST3 + ST4, pmol / l 33.59 ± 5.12 32.87 ± 4.32 25.15 ± 3.78 28.64 ± 5.03 31.69 ± 5.08 38.87 ± 4.95 The ratio of CT3 / CT4 0.67 ± 0.07 0.68 ± 0.03 0.53 ± 0.06 0.67 ± 0.04 0.80 ± 0.07 0.89 ± 0.06 Estradiol, nmol / L 0.41 ± 0.03 0.43 ± 0.06 0.42 ± 0.05 0.65 ± 0.05 0.45 ± 0.07 0.51 ± 0.06 Testosterone, nmol / L 14.18 ± 3.01 13.98 ± 2.89 13.97 ± 4.01 17.26 ± 3.79 17.92 ± 5.21 30.49 ± 7.50 Estradiol-Testosterone Ratio 0.03 ± 0.02 0.03 ± 0.06 0.03 ± 0.06 0.04 ± 0.05 0.02 ± 0.03 0.01 ± 0.02

The positive effect of drinking calcium carbonate micellate “Aleksanat Zoo” on the content of sex hormones in bovine serum was established. So, the testosterone level in the experimental group of animals 30 days after the start of the experiment increased by 23.5% and amounted to 17.26 ± 3.79 nmol / l, while in the intact group of bulls it decreased from 14.18 ± 3.01 nmol / L to 13.97 ± 4.02 nmol / L. After 60 days, the testosterone level in the experimental group increased more than 2 times compared with the initial data and amounted to 30.49 ± 7.52 nmol / l).

Positive dynamics was also noted in the serum estradiol content: 30 days after the start of the experiment, the level of this hormone significantly increased in the experimental group - 1.5 times and amounted to 0.65 ± 0.05 nmol / l, after 2 months it was higher in compared with the control by 13.5% (0.51 ± 0.03 nmol / L).

Small but still significant amounts of estradiol, the female sex hormone, are produced in the testes. Most of the estradiol formed in males is the result of peripheral aromatization of testosterone and androstenedione. It is believed that the synthesis of estradiol involves Leydig cells, Sertoli cells and seminiferous tubules.

Low estradiol levels occur with hypothyroidism. (Rommerts, 2004). Estrogen deficiency can cause significant impairment of spermatogenesis and fertilization, increase testosterone and LH, reduce bone mass and increase body fat, which indicates the importance of estrogen in the regulation of bone mass, gonadotropin, body composition and spermatogenesis (Smith et al., 1994; Carani et al., 1997).

Thus, additional watering to the bulls-manufacturers of the drug "Aleksanat Zoo" according to the proposed method leads to normalization of metabolic processes, increased activity of the thyroid gland and gonads.

When studying the effect of drinking Aleksanat Zoo on the reproductive ability of bulls, we took into account the indicators of native sperm for the periods: the first period - before the drug was thirsty in the experimental group (average ejaculate volume and sperm concentration 30 days before the experiment), the second period - 90 days - the period of drinking, the third period - 90 days after drinking.

Table 5 The effect of drinking the drug "Aleksanat Zoo" on the sperm productivity of bulls (I period - initial data; II period - the period of drinking, III period - after drinking) Name of the month The volume of ejaculate, ml Sperm concentration billion / ml Group Group Control Experienced Control Experienced I period Initial data (a month before drinking) 4.32 ± 0.36 4.06 ± 0.20 1.05 ± 0.23 0.99 ± 0.70 II period 30 days after drinking 4.12 ± 0.33 4.62 ± 0.26 1.03 ± 0.47 1.12 ± 0.77 60 days after the start of drinking 4.24 ± 0.46 5.52 ± 0.17 0.97 ± 0.25 1.54 ± 0.49 90 days after the start of drinking 4.15 ± 0.29 5.21 ± 0.28 0.92 ± 0.18 1.43 ± 0.52 III period 30 days after the end of drinking 4.34 ± 0.22 5.37 ± 0.36 0.95 ± 0.22 1.57 ± 0.55 60 days after the end of drinking 4.23 ± 0.27 5.49 ± 0.40 1.02 ± 0.27 1.61 ± 0.52 90 days after the end of drinking 4.13 ± 0.23 4.43 ± 0.38 1.01 ± 0.27 1.15 ± 0.51

The results of scientific and industrial experience show that the additional introduction of minerals into the diet of bulls by feeding “Aleksanat-Zoo” positively affects their reproductive ability and the quality of sperm production. So the volume of ejaculate in the bulls of the experimental group for the second period was higher by 24.3%. So a month after taking the drug, the volume of ejaculate increased from 4.06 ± 0.20 to 4.62 ± 0.26 ml, after 60 days to 5.52 ± 0.17 ml, and after 90 days it amounted to 5.21 ± 0.28 ml. In the third, the volume of ejaculate in the bulls of the experimental group increased by 21.3% compared with the initial data and by 13.1% and 8.0%, respectively, compared with the control.

The concentration of sperm in the ejaculate by analogy: in the second period was higher by 37.7%, in the third - by 45.4% compared with the original data and by 14.7 and 18.1%, respectively, compared with the control.

Thus, the drinking of the drug “Aleksanat Zoo” had a positive effect on the main indicators of native sperm of bulls - the volume of ejaculate and the concentration of sperm in the ejaculate.

The economic efficiency of the drug was calculated at the cost obtained additionally by increasing the number of products. The following formulas were used:

E _p = E _a / _{W, in} which

E _p - economic efficiency per ruble of costs;

E _in - the economic effect obtained as a result of the use of the drug "Aleksanat Zoo", rub .;

З _в - expenses for the purchase of the drug "Aleksanat Zoo", rub.

E _c = C * (K1 * K2 * K3), where

C is the selling price of 1 sperm dose;

K1 - the number of bulls in the experimental group;

K2 - the number of ejaculates received from 1 bull for 90 days of experience;

K3 - the number of additionally frozen sperm doses per take from 1 bull in the experimental group.

Sv = C * (M * D * B), where

C - the cost of the drug "Aleksanat Zoo", 1 l;

M is the number of bulls in the group;

D - daily dose of the drug "Aleksanat Zoo", kg;

B - the duration of the use of the drug "Aleksanat Zoo", days.

Table 6 The economic efficiency of drinking the drug "Aleksanat Zoo" bulls. Indicator Control group Experienced group + - The number of bulls in the group 6 6 Duration of observations, days 180 180 The cost of 1 liter of "Aleksanat Zoo", rubles. - 300 The cost of the course per animal, rub. - 324 +324 The number of ejaculates per bull 97 102 +5 Doses received for the observation period 130197 134490 +4293 Doses received on average per bull 21699 22415 +716 The average cost of sperm doses, rubles 165.0 160,0 -5

Over the period of the experiment, biological sperm rejection in the second group was reduced by 0.21%, the yield of quality sperm doses over 180 days (for the second and third periods) increased by 4293 doses or 3.2% compared to the control. On average, more than 716 sperm doses were received per bull. By increasing the yield of quality doses, an additional 35.87 thousand rubles were received. profit, the economic effect on the ruble of costs amounted to 18.18 rubles.

Additional watering to the bulls-producers of the drug "Aleksanat Zoo" leads to the normalization of metabolic processes, increased activity of the thyroid and genital glands.

The use of the drug Aleksanat Zoo in bull diets had a positive effect on the volume and quality of sperm production of bulls, providing an increase in ejaculate volume by 23% and sperm concentration in the ejaculate by 10%.

The use of "Aleksanat Zoo" helps to reduce biological sperm defects by 0.21%, increase the yield of quality doses by 3.2%.

The economic effectiveness of the drug "Aleksanat Zoo" to increase the reproductive potential of breeding bulls by 1 ruble of costs amounted to 18.18 rubles.

To increase the reproductive potential of breeding bulls and improve the quality of sperm production, we recommend daily drinking Aleksanat Zoo (calcium carbonate micelate) at a dose of 300 ml for three months.

Claims (1)

A method of increasing the reproductive ability of bulls, including the introduction of a mineral feed additive into the diet, characterized in that as a stimulating mineral feed additive, bulls are daily fed an aqueous solution of the calcium carbonate micellate “Aleksanat Zoo”, the drug being administered at a dose of 300 g per head for three months, while before administration, the drug is diluted with water in a ratio of 1:50.