RU2538654C2 - Обогащенные ангиогенином фракции молока - Google Patents
Обогащенные ангиогенином фракции молока Download PDFInfo
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- RU2538654C2 RU2538654C2 RU2010150896/10A RU2010150896A RU2538654C2 RU 2538654 C2 RU2538654 C2 RU 2538654C2 RU 2010150896/10 A RU2010150896/10 A RU 2010150896/10A RU 2010150896 A RU2010150896 A RU 2010150896A RU 2538654 C2 RU2538654 C2 RU 2538654C2
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- angiogenin
- milk
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- A23J1/20—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from milk, e.g. casein; from whey
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Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2008902373A AU2008902373A0 (en) | 2008-05-14 | Enriched milk fraction | |
| AU2008902375 | 2008-05-14 | ||
| AU2008902369A AU2008902369A0 (en) | 2008-05-14 | Enriched milk fraction | |
| AU2008902366A AU2008902366A0 (en) | 2008-05-14 | Enriched milk fraction | |
| AU2008902373 | 2008-05-14 | ||
| AU2008902375A AU2008902375A0 (en) | 2008-05-14 | Enriched milk fraction | |
| AU2008902369 | 2008-05-14 | ||
| AU2008902366 | 2008-05-14 | ||
| PCT/AU2009/000604 WO2009137881A1 (en) | 2008-05-14 | 2009-05-14 | Angiogenin-enriched milk fractions |
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| RU2010150896A RU2010150896A (ru) | 2012-06-20 |
| RU2538654C2 true RU2538654C2 (ru) | 2015-01-10 |
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| EP (1) | EP2293678A4 (https=) |
| JP (1) | JP5717625B2 (https=) |
| CN (2) | CN106699869A (https=) |
| AU (1) | AU2009246054B2 (https=) |
| BR (1) | BRPI0911957A2 (https=) |
| CA (1) | CA2723994C (https=) |
| NZ (1) | NZ589311A (https=) |
| RU (1) | RU2538654C2 (https=) |
| WO (1) | WO2009137881A1 (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2717340C1 (ru) * | 2018-12-20 | 2020-03-23 | Общество с ограниченной ответственностью "НПК "Альтернативные технологии" | Способ получения лактоферрина |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2009246053B2 (en) | 2008-05-14 | 2014-07-24 | Agriculture Victoria Services Pty Ltd. | Use of angiogenin or angiogenin agonists for treating diseases and disorders |
| AU2013204721B2 (en) * | 2008-05-14 | 2016-08-18 | Agriculture Victoria Services Pty Ltd | Methods for treating cachexia |
| EP2501411A4 (en) * | 2009-11-18 | 2013-07-03 | Murray Goulburn Coop Co Ltd | PROCESS FOR IMPROVED ORAL ADMINISTRATION |
| US20120331575A1 (en) * | 2009-11-18 | 2012-12-27 | Agriculture Victoria Services Pty Ltd | Transgenic non-human animals |
| PT2739157T (pt) * | 2011-08-03 | 2018-01-09 | Prolacta Bioscience Inc | Microfiltração de leite humano para reduzir a contaminação bacteriana |
| JP5955632B2 (ja) * | 2012-05-02 | 2016-07-20 | 雪印メグミルク株式会社 | ヒアルロン酸産生促進剤 |
| AU2013204740C1 (en) * | 2012-05-10 | 2015-10-01 | Agriculture Victoria Services Pty Ltd | Methods of treating cancer using angiogenin or an angiogenin agonist |
| CN107259269A (zh) * | 2012-07-31 | 2017-10-20 | 雪印惠乳业株式会社 | 饮料及其制造方法 |
| JP6124418B2 (ja) * | 2012-07-31 | 2017-05-10 | 雪印メグミルク株式会社 | 飲料及びその製造方法 |
| JP6203723B2 (ja) * | 2012-07-31 | 2017-09-27 | 雪印メグミルク株式会社 | 骨疾患の予防又は治療用タンパク質素材及びその製造方法 |
| AU2013204858B2 (en) * | 2012-10-08 | 2015-06-25 | Saputo Dairy Australia Pty Limited | Improved process for purifying milk proteins and products thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2109748C1 (ru) * | 1996-12-27 | 1998-04-27 | Московский государственный университет прикладной биотехнологии | Способ выделения ангиогенина |
| RU2175195C1 (ru) * | 2000-06-23 | 2001-10-27 | Московский государственный университет прикладной биотехнологии | Способ выделения белковой фракции, обогащенной ангиогенином |
| RU2183935C2 (ru) * | 2000-04-06 | 2002-06-27 | Московский государственный университет прикладной биотехнологии | Способ получения биологически активной добавки "милканг" и полученная этим способом бад "милканг" |
| RU2204262C2 (ru) * | 2001-07-06 | 2003-05-20 | Московский государственный университет прикладной биотехнологии | Способ получения биологически активного белкового концентрата, обогащенного панкреатической рибонуклеазой а, ангиогенином и лизоцимом, из молочного ультрафильтрата |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3149060A (en) * | 1959-01-13 | 1964-09-15 | Int Minerals & Chem Corp | Electrophoresis method and apparatus |
| FR2623509B1 (fr) * | 1987-11-19 | 1990-04-20 | Centre Nat Rech Scient | Proteine de 17 kd a action angiogenique, son procede d'isolement a partir de lait de mammiferes, compositions therapeutiques la contenant, procede de detection et/ou de dosage et reactifs immunologiques de detection et de dosage des angiogenines de mammiferes, de leurs homologues et de leurs fragments |
| JP2745076B2 (ja) * | 1990-04-11 | 1998-04-28 | 雪印乳業株式会社 | κ―カゼイングリコマクロペプチドの製造法 |
| JP2900953B2 (ja) * | 1990-11-30 | 1999-06-02 | 雪印乳業株式会社 | α―ラクトアルブミン含有量の高い乳画分の製造法及び該画分を含有する製品 |
| JP3225080B2 (ja) * | 1992-03-30 | 2001-11-05 | 雪印乳業株式会社 | κ−カゼイングリコマクロペプチド含有量の高い組成物の製造方法 |
| US5352468A (en) * | 1992-09-30 | 1994-10-04 | Schroder Bruce G | Process concentrate and diary products |
| JP2568151B2 (ja) * | 1992-10-24 | 1996-12-25 | 雪印乳業株式会社 | アンジオジェニンの単離方法 |
| JP3544213B2 (ja) * | 1993-03-31 | 2004-07-21 | 雪印乳業株式会社 | インスリン様増殖因子−１含有組成物の製造法 |
| JP2572931B2 (ja) * | 1993-04-28 | 1997-01-16 | 雪印乳業株式会社 | 美白剤 |
| JP3501495B2 (ja) * | 1994-03-31 | 2004-03-02 | 雪印乳業株式会社 | ウシインスリン様増殖因子−１含有組成物の製造法 |
| US6268487B1 (en) * | 1996-05-13 | 2001-07-31 | Genzyme Transgenics Corporation | Purification of biologically active peptides from milk |
| JP3055476B2 (ja) * | 1996-11-28 | 2000-06-26 | 株式会社村田製作所 | 可変抵抗器 |
| RU2110066C1 (ru) | 1996-12-27 | 1998-04-27 | Московский государственный университет прикладной биотехнологии | Способ количественного определения ангиогенина в биологическом сырье |
| NL1005677C2 (nl) * | 1997-03-27 | 1998-09-29 | Campina Melkunie Bv | Werkwijze voor het winnen van groeifactoren, of een samenstelling die één of meer groeifactoren bevat, uit melk of een derivaat daarvan. |
| US20040117863A1 (en) * | 1998-09-18 | 2004-06-17 | Edge Michael D. | Transgenically produced fusion proteins |
| CA2516836A1 (en) * | 2003-02-24 | 2004-09-10 | Gtc Biotherapeutics, Inc. | Methods of tangential flow filtration and an apparatus therefore |
| EP1628725A4 (en) * | 2003-04-22 | 2008-01-09 | Rensselaer Polytech Inst | MICRO-FILTRATION AND / OR ULTRA-FILTRATION PROCESS FOR RECOVERING TARGET MOLECULES FROM POLYDISPERSED LIQUIDS |
| WO2004094207A1 (en) | 2003-04-23 | 2004-11-04 | Eus System Ab | Arrangement for a washer system for vehicles and a method for its production |
| JP4698935B2 (ja) | 2003-05-07 | 2011-06-08 | 雪印乳業株式会社 | 皮膚コラーゲン産生促進剤 |
| CA2586515A1 (en) * | 2003-11-05 | 2005-05-26 | Exact Sciences Corporation | Repetitive reversed-field affinity electrophoresis and uses therefor |
| WO2006116381A2 (en) * | 2005-04-22 | 2006-11-02 | Imquest Biosciences, Inc. | Plasma or serum fraction for treatment or prevention of abnormal cell proliferation |
| FR2889068B1 (fr) * | 2005-07-29 | 2012-03-02 | Cie Laitiere Europeenne | Nouvelles fractions proteiques laitieres et leur utilisation pour la prevention ou le traitement des maladies inflammatoires chroniques |
| US7531632B2 (en) * | 2006-02-16 | 2009-05-12 | Gtc Biotherapeutics, Inc. | Clarification of transgenic milk using depth filtration |
| JP5276004B2 (ja) | 2006-11-10 | 2013-08-28 | マリー ゴールバーン シーオー−オペレイティブ シーオー．リミテッド | アンギオゲニンの調製方法 |
| US7601689B2 (en) * | 2007-04-12 | 2009-10-13 | Naidu Lp | Angiogenin complexes (ANGex) and uses thereof |
| US8828698B2 (en) * | 2007-04-12 | 2014-09-09 | Naidu Lp | Immobilized angiogenin mixtures and uses thereof |
-
2009
- 2009-05-14 EP EP09745303A patent/EP2293678A4/en not_active Withdrawn
- 2009-05-14 NZ NZ589311A patent/NZ589311A/xx not_active IP Right Cessation
- 2009-05-14 AU AU2009246054A patent/AU2009246054B2/en not_active Ceased
- 2009-05-14 CA CA2723994A patent/CA2723994C/en not_active Expired - Fee Related
- 2009-05-14 JP JP2011508770A patent/JP5717625B2/ja not_active Expired - Fee Related
- 2009-05-14 US US12/992,530 patent/US9247766B2/en not_active Expired - Fee Related
- 2009-05-14 CN CN201710110192.5A patent/CN106699869A/zh active Pending
- 2009-05-14 CN CN2009801274364A patent/CN102088862A/zh active Pending
- 2009-05-14 BR BRPI0911957A patent/BRPI0911957A2/pt not_active Application Discontinuation
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- 2009-05-14 RU RU2010150896/10A patent/RU2538654C2/ru not_active IP Right Cessation
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2109748C1 (ru) * | 1996-12-27 | 1998-04-27 | Московский государственный университет прикладной биотехнологии | Способ выделения ангиогенина |
| RU2183935C2 (ru) * | 2000-04-06 | 2002-06-27 | Московский государственный университет прикладной биотехнологии | Способ получения биологически активной добавки "милканг" и полученная этим способом бад "милканг" |
| RU2175195C1 (ru) * | 2000-06-23 | 2001-10-27 | Московский государственный университет прикладной биотехнологии | Способ выделения белковой фракции, обогащенной ангиогенином |
| RU2204262C2 (ru) * | 2001-07-06 | 2003-05-20 | Московский государственный университет прикладной биотехнологии | Способ получения биологически активного белкового концентрата, обогащенного панкреатической рибонуклеазой а, ангиогенином и лизоцимом, из молочного ультрафильтрата |
Non-Patent Citations (1)
| Title |
|---|
| ФЕДОРОВА Т.В. и др., Панкреатические рибонуклеазы молочного пермеата, Биотехнология, 2000, N6, стр. 61-65 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2717340C1 (ru) * | 2018-12-20 | 2020-03-23 | Общество с ограниченной ответственностью "НПК "Альтернативные технологии" | Способ получения лактоферрина |
Also Published As
| Publication number | Publication date |
|---|---|
| EP2293678A4 (en) | 2011-06-22 |
| EP2293678A1 (en) | 2011-03-16 |
| US9247766B2 (en) | 2016-02-02 |
| CA2723994A1 (en) | 2009-11-19 |
| US20110123514A1 (en) | 2011-05-26 |
| JP5717625B2 (ja) | 2015-05-13 |
| NZ589311A (en) | 2012-08-31 |
| AU2009246054A1 (en) | 2009-11-19 |
| JP2011519962A (ja) | 2011-07-14 |
| RU2010150896A (ru) | 2012-06-20 |
| BRPI0911957A2 (pt) | 2016-08-23 |
| CA2723994C (en) | 2018-05-01 |
| WO2009137881A1 (en) | 2009-11-19 |
| CN102088862A (zh) | 2011-06-08 |
| CN106699869A (zh) | 2017-05-24 |
| AU2009246054B2 (en) | 2014-01-23 |
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