RU2520327C1 - Inoculation loop - Google Patents

Abstract

FIELD: biotechnologies.

SUBSTANCE: invention proposes an inoculation loop for cultivation of microorganisms. Bacteriological loop includes a handle on one side and a detachable working element on the other side. The working element represents a metal rod with an eyehole on its end. The working element near a coupling is equipped with metal limiters located perpendicular to the working element. Metal guides are fixed on ends of metal limiters. Guides are located along the metal rod with an eyehole at the distance from it, which is equal to external radius of test tube cross-section.

EFFECT: improvement of characteristics.

3 dwg, 1 tbl

Description

The invention relates to microbiology, and in particular to laboratory tools for the cultivation of microorganisms. The most effective application of the bacteriological loop when sowing microbial cultures with an injection into tubes with solid nutrient media is a column in order to accumulate biomass.

Known bacteriological loop, which is a handle on one side and a removable working element in the form of a wire of easily modeled metal. Its free end is bent in the form of an abalone and used for bacteriological work to transfer microbiological material (Workshop on Veterinary Microbiology and Immunology / T.S. Kostenko, E.I. Skarshevskaya, S.S. Gitelson. - M .: Agropromizdat, 1989. - S. 23. - [1]).

Also known is a bacteriological loop containing a handle on one side and a working element in the form of a metal rod with an eye at its end. It is used when reseeding microbial cultures from one tube to another. Sowing with an injection is done using a bacteriological loop, which is introduced into a column of a dense nutrient medium (Workshop on Veterinary Microbiology and Immunology / V.N. Kislenko. - M .: KolosS, 2005. - P.29-31. - [2]).

A disadvantage of the known bacteriological loops is that the manipulation of transferring microbial cultures from one tube to another requires the concentration of efforts of laboratory workers and their concentration to introduce the working element of the bacteriological loop strictly in the center of the column of the nutrient medium. Performing such an accurate job, the lab worker needs to keep the brush tense. Concentration and the implementation of actions to aim at a given point of the working element of the bacteriological loop entails additional time spent on the manipulation.

In this case, it is possible that the loop moves away from the center of the cross section of the tube and, accordingly, from the center of the solid nutrient medium. The zone of unintentional introduction of the pathogen culture into unplanned areas of solid nutrient medium increases. Subsequently, this can distort the characteristic features of the growth of colonies of various microorganisms, which reduces the possibility of a visual assessment of the results of the growth of microorganisms on a nutrient medium. During experimental studies, various samples are placed in different conditions.

In addition, the concentration of researchers is necessary in determining the depth of introduction of microbial cultures into the nutrient medium, namely, as is generally accepted at 2/3 of the height of the medium column. It is often difficult for a laboratory worker to balance efforts when introducing a bacteriological loop deep into a solid nutrient medium. Such a manipulation with its repeatability cannot be standardly performed on all samples, which can also distort the research results. As a rule, such results are not taken into account and the work is performed repeatedly. The latter is often impossible to duplicate under experimental conditions, for example, when analyzing the microbial contamination of the wound discharge at certain times after surgery, for timely and justified postoperative antibiotic therapy.

The objective of the proposed solution is the sowing of microbial cultures in the center of a solid nutrient medium and at the same depth in all the studied samples.

The task is achieved due to the fact that the known bacteriological loop containing a handle on one side and a removable working element on the other hand in the form of a metal rod with an eye at its end, fixed in the handle by a sleeve, according to the invention, the working element near the sleeve is additionally equipped with metal stops located perpendicular to the working element, while at the ends of the metal stops metal guides are fixed, which are located along the metal Reaper with an eye and at a distance from it equal to the outer radius of the cross section of the tube.

The technical essence of the proposed bacteriological loop is presented in the drawing, where:

figure 1 shows the proposed bacteriological loop (General view);

figure 2 - section aa in figure 1;

figure 3 - bacteriological loop in the working position.

The bacteriological loop contains a handle 1, a removable working element in the form of a metal rod 2 with an eye 3 at its end, fixed in the handle 1 with a sleeve 4. At the same time, the working element is equipped with stops 5 with guides 6, which during operation are located outside of the tube 7.

The bacteriological loop works as follows. A tube with a microbial culture and a tube for reseeding with a solid nutrient medium are taken in the left hand. Among solid nutrient media, meat-peptone agar (MPA) and meat-peptone gelatin (MPG) are often used.

Both tubes are held in one hand in an inclined state, while their tubes should be in line and they should be directed towards the burner flame. The burner must be placed between the hands of the laboratory worker. The test tube with the source material is placed below the test tube with a solid nutrient medium into which microbial culture is planned. Between the tubes there should be a gap for the passage of guides 6 between them when introducing a metal rod 2 with the eye 3 of the bacteriological loop into a tube with a microbial culture, or into a tube with a solid nutrient medium.

The bacteriological loop is held by the handle 1 with the right hand (like a writing pen). Before taking the microbial culture, the working element of the bacteriological loop is carefully burned in the flame of the burner, that is, it is flamed. There is no need to avoid the contact of the guides 6 with the open flame of the burner, as they are metal. Without releasing the tubes from the hands, carefully near the flame they are opened with the free fingers (little finger and ring finger) of the right hand. At the same time, their stoppers are pressed with the little finger of the right hand to the palm of the hand, and after removing them from the tubes, they are held in the hand.

To take a microbial culture, a profiled metal rod 2 with an ear 3 of a bacteriological loop is introduced into the tube lumen, not reaching the microbiological material, where this working element is cooled. Next, the metal rod 2 with the eye 3 is directed deep into the tubes, while the guides 6 are aimed so that they enclose the tube around its circumference. Due to the bevel at the ends of the guides 6 facilitates their crawl onto the tube from the outside around its circumference. The advancement of the working element of the bacteriological loop into the test tube is continued to the required depth and, if necessary, then to the stop 5.

Then the bacteriological loop contacts the culture and capture a drop of microbiological material. The culture is retained not only on the surface of the metal rod 2, but also in its ear 3. After this, the culture is removed from the test tube so that it does not pass over the flame of the burner.

When reseeding from one tube to another, the metal rod 2 with the eye 3 of the bacteriological loop is sent to a tube with a solid nutrient medium. After insertion of the eyelet 3 into the lumen of the tube, the direction to the working element of the bacteriological loop is attached with the index finger located on top of the handle 1 near the sleeve 4. In this case, the guides 6 are arranged so that they are placed around the circumference of the tube. The bevels at the ends of the guides 6 facilitate the placement of the latter on the tube from the outside around its circumference. The advancement of the metal rod 2 with the eye 3 of the bacteriological loop into the test tube continues until it stops in the stoppers 5. This ensures the introduction of microbial cultures into the solid nutrient medium to a predetermined depth - 2/3 of the height of its column.

Microbes remain on the path of the working element of the bacteriological loop deep into the solid nutrient medium; they can grow by injection. That is, the microbial culture is inoculated with an injection into a test tube with a solid nutrient medium column. Then the bacteriological loop is taken out of the test tube. Both tubes are closed with stoppers and put in a tripod.

After using the bacteriological loop, it is sterilized both assembled and disassembled. In the latter case, the clutch 4 is unscrewed and the working element is released from the handle 1 in the form of a metal rod 2 with an eye 3, as well as with stops 5 and guides 6. A collapsible bacteriological loop is also designed to replace its worn out elements. So, for example, flaming of the working element of the bacteriological loop can lead to wear (burning out) of the metal rod 2, eyelet 3 or guides 6.

In repeated tests of the proposed bacteriological loop, it was revealed not only its unobstructed passage deep into the solid nutrient medium, but also the penetration of ear 3 with microbial cultures into this nutrient medium to a standard set depth - 2/3 of its column height, which is facilitated by the limiters 5.

The device of the bacteriological loop allows the researcher to work equally confidently with both his right and left hands.

The application of the proposed bacteriological loop allows you to:

- increase the controllability and convenience of the laboratory instrument;

- observe the technique of sowing microbial cultures strictly in the center of a solid nutrient medium and at the same depth - 2/3 of the height of its column;

- facilitate the reseeding of microbial cultures;

- reduce the time for manipulation;

- reduce the tension of the mode of operation of the researcher;

- observe the technique of cultivating microorganisms;

- increase the readability and accuracy of laboratory research results;

- determine the timely and justified postoperative antibiotic therapy;

- eliminate the need for repeated studies.

This significantly reduces the complexity of reseeding microbial cultures and increases the effectiveness of laboratory research (see table).

Table Bacteriological loop Number of crops Doubtful Results Repeated studies Proposed 35 - - Famous 35 8 8

As can be seen from the table, the use of the proposed bacteriological loop is more effective when reseeding microbial cultures in solid nutrient media compared to the known one, and allows to exclude repeated studies.

The proposed bacteriological loop was successfully applied in the study of microorganisms on the basis of the Department of Epizootology and Therapy FSBEI HPE Orel GAU and the Department of Clinical Veterinary Medicine FSBEI HPE "Peoples' Friendship University of Russia".

When using the proposed bacteriological loop, a decrease in the complexity of working with this laboratory instrument was noted. At the same time, microbial cultures were sown strictly in the center of a solid nutrient medium, as well as to the same depth in all samples.

Claims (1)

A bacteriological loop containing a handle on one side and a removable working element on the other hand in the form of a metal rod with an eye at its end, fixed to the handle by a sleeve, characterized in that the working element near the sleeve is additionally equipped with metal stops located perpendicular to the working element, metal guides are fixed at the ends of the metal stops, which are located along the metal rod with the eye and at a distance from it equal to the outer radius along cross section of a test tube.

Images