SU628171A1 - Device for inoculation - Google Patents

Device for inoculation

Info

Publication number
SU628171A1
SU628171A1 SU772469809A SU2469809A SU628171A1 SU 628171 A1 SU628171 A1 SU 628171A1 SU 772469809 A SU772469809 A SU 772469809A SU 2469809 A SU2469809 A SU 2469809A SU 628171 A1 SU628171 A1 SU 628171A1
Authority
SU
USSR - Soviet Union
Prior art keywords
agar
needle
bacteria
inoculation
sowing
Prior art date
Application number
SU772469809A
Other languages
Russian (ru)
Inventor
Вениамин Иванович Седов
Original Assignee
Ивановский государственный медицинский институт
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ивановский государственный медицинский институт filed Critical Ивановский государственный медицинский институт
Priority to SU772469809A priority Critical patent/SU628171A1/en
Application granted granted Critical
Publication of SU628171A1 publication Critical patent/SU628171A1/en

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Description

II

Изс ретенне относитс  к микробиологической 1ромышленностн, а нмеиио к устрой-/ ствам дл  проведени  микробиологических лабораторных исследований.Research is related to the microbiological industry, and it is not one of the devices for carrying out microbiological laboratory tests.

Известно устройство дл  посева бактерий уколом в агар на заданную глубину, состо щее из иг oдepжaтeл  и бактериальной нглы {.A device is known for planting bacteria by injecting agar to a predetermined depth, which consists of a protective agent and a bacterial ngla {.

Однако после каждого посева уколом в агар, перед вз тием культуры, нгЛу с целью исключени  ее загр знени  другими микроорганизмами необходимо прокаливать на спиртовке или газовой горелке, на что расходуетс  много времени.However, after each inoculation of the agar in the agar, before taking the culture, it is necessary to ngLu to exclude its contamination with other microorganisms on a spirit lamp or gas burner, which takes a lot of time.

При посеве микроорганизмов уколом в агар на чашки Петри, например, дл  бактериоцинотипнрованл  бактерий и обнаружени  бактериоциногениых культур прокалывают , слой агара до дна чашки. В результате микрокапилл рности засе нные микробы распростран ютс  между дном чашки и слоем агара. При этом они растут не только по уколу, но и под средой, образу  различной величины н формы газоны, которые в значительной степени искажают результаты опыта.When sowing microorganisms by injecting agar on Petri dishes, for example, for bacterioccinotyped bacteria and detecting bacteriocytogenogenic cultures, a layer of agar is pierced to the bottom of the cup. As a result of the microcapillaries, the seeded microbes spread between the bottom of the dish and the agar layer. At the same time, they grow not only after a shot, but also under the medium, forming a lawn of various size and shape, which substantially distort the results of the experiment.

Целью изобретени   вл етс  упрощение и ускорение процесса посева одной культуры бактерий с бульона в агар на несколько чашек Петрн на заданную глубину и обеспечение стерильности посева.The aim of the invention is to simplify and speed up the process of sowing a single culture of bacteria from broth into agar into several Petrin plates to a predetermined depth and to ensure sterility of sowing.

Дл  этого предлагаемое устройство снабжено подвижным ограничителем в виде треугольной рамки с полочкой, а игла вьтолнена изогнутой.For this, the proposed device is equipped with a movable stopper in the form of a triangular frame with a shelf, and the needle is filled with a curved.

На чертеже представлено предлагаемое устройство. Оно состоит из иглодержател  I, двух гаек 2 дл  установки глубины укола в агар, конической гайки дл  креплени  иглы в иглодержателе 3, бактериальной седловидио изогнутой иглы 4, подвижного ограничител , выполненного в виде треугольной рамки 5 с полочкой 6 и фиксирующего винта 7Устройство работает следующим образом .The drawing shows the proposed device. It consists of a needle holder I, two nuts 2 for setting the injection depth in agar, a conical nut for fastening the needle in the needle holder 3, a bacterial saddle and curved needle 4, a movable stopper made in the shape of a triangular frame 5 with a shelf 6 and a fixing screw 7 The device works as follows .

Перед посевом бактерий подвижный ограничитель перемещают на свободный конец иглодержател , иглу стерилизуют над пламенем горелки, охлаждают и нанизывают на нее в пробирке каплю бульонной ку 1ьтуры. Чтобы нижн   сторона треугольной рамки ограничител  не мешала нанизыванию в npovBefore sowing the bacteria, the movable stopper is moved to the free end of the needle holder, the needle is sterilized over the burner flame, cooled, and a drop of broth powder is applied to it in a test tube. So that the bottom side of the triangular frame of the limiter does not interfere with stringing in npov

SU772469809A 1977-04-04 1977-04-04 Device for inoculation SU628171A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SU772469809A SU628171A1 (en) 1977-04-04 1977-04-04 Device for inoculation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SU772469809A SU628171A1 (en) 1977-04-04 1977-04-04 Device for inoculation

Publications (1)

Publication Number Publication Date
SU628171A1 true SU628171A1 (en) 1978-10-15

Family

ID=20702404

Family Applications (1)

Application Number Title Priority Date Filing Date
SU772469809A SU628171A1 (en) 1977-04-04 1977-04-04 Device for inoculation

Country Status (1)

Country Link
SU (1) SU628171A1 (en)

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