RU112613U1 - DEVICE FOR SOWING TEST STRAINS - Google Patents

Abstract

A device for seeding test strains containing a bacteriological loop fixed in a loop holder, characterized in that a tip made of polypropylene is used as a loop holder, a loop is inserted into the working hole of which a loop is inserted, secured with expanding wire antennae, and the opposite end is put on a landing cone 8-channel BIOHIT pipette dispenser.

Description

The utility model relates to medical microbiological diagnostic equipment and can be used to study the antagonistic activity of microbial cultures, in particular, in the production and control of probiotic preparations.

A known method for determining the antagonistic activity of microbial cultures by the method of delayed antagonism by seeding test cultures with transverse strokes using a bacteriological loop, which is fixed in the collet tip of the loop holder (General and sanitary microbiology with the technique of microbiological research / edited by A.S. Labin, L. P. Blinkova, A.S. Yeshchina. - M .: Medicine, 2004. - p.122).

Disadvantages: low productivity due to the need to apply a large number of crops (at least 6-8) of different test cultures for each Petri dish.

EFFECT: reduced sowing time by simultaneously carrying out the required number of crops (from 2 to 8) on the surface of a dense nutrient medium, increasing the accuracy of sowing.

This task is achieved using a device, which is a bacteriological loop, fixed with an expandable antennae in tips made of polypropylene, which are worn on the landing cones of an 8-channel BIOHIT pipette dispenser (Finland) with a dosage range of 5-50 μl.

BIOHIT pipette dispenser is used for dispensing liquids in immunological, microbiological and biochemical studies. For crops on solid nutrient media, a pipette dispenser has not been previously used.

A useful model is shown in the drawing, where in Fig. 1 its general view is given, and in Fig. 2 a tip with a bacteriological loop is shown.

The dispenser is structurally a handle (1), on the upper end of which there is a plunger rod (2). On the side surface of the handle there is a volume indication window (3). The tip ejector lever (4) is also located in the upper part of the handle housing. The lower end of the handle is connected to the body-holder of the piston channels (5), on which the landing cones (6) for the tips (7) are located.

The loop (8) is made of nichrome filament with a thickness of 0.1-0.15 mm and is fixed in the tip (7) using an expandable antennae (9). Before use, handpieces with hinges are autoclaved at 120 ° C.

The device is used as follows:

Sterilized tips (7) with loops (8) are put on the landing cones of the dispenser (6). In the wells of a sterile 96-well plate, broth cultures of test strains, for example, Staphylococcus aureus, are added. Using the device, 5-10 μl of cultures are taken from the wells, transferred to the surface of the culture medium and distributed in the form of transverse streaks.

A positive effect of using the proposed device is the ability to quickly simultaneously apply several test strains (from 2 to 8), as well as to increase the accuracy of dosing of applied microbial cultures and standardize the distance between crops (strokes).

Claims (1)

A device for sowing test strains containing a bacteriological loop fixed in a loop holder, characterized in that a tip made of polypropylene is used as a loop holder, a loop fixed to it with an expandable wire antenna and a looped end is worn on the landing cone 8-channel pipette dispenser BIOHIT.