CN202188973U - Injector type agar stamping sampler - Google Patents
Injector type agar stamping sampler Download PDFInfo
- Publication number
- CN202188973U CN202188973U CN2011203304570U CN201120330457U CN202188973U CN 202188973 U CN202188973 U CN 202188973U CN 2011203304570 U CN2011203304570 U CN 2011203304570U CN 201120330457 U CN201120330457 U CN 201120330457U CN 202188973 U CN202188973 U CN 202188973U
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- sampling
- sampling pipe
- culture medium
- sampler
- skin
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Abstract
The utility model discloses an injector type agar stamping sampler, comprising a sampling pipe; a push-and-pull rod is arranged in the sampling pipe, the rear end of the push-and-pull rod stretches out of the sampling pipe, and a cover is arranged at the front end of the sampling pipe. The sampler is used as follows: firstly, sterilizing the sampler, pouring the dissolved agar culture medium into the sampling pipe, and covering the sampling pipe to cool; when the skin is needed to be sampled, the cover is taken down, the agar culture medium is pushed out, a skin surface sampling area is stamped at the top surface of the agar culture medium in a pressing manner, and the slight rotation is performed, so as to enable the sampling area to be fully contacted with the skin; the exposed agar culture medium is cut along the front end of the sampling pipe by a scalpel, the sampling surface of the cut agar culture medium is arranged in an aseptic plate upwards, the operation is repeated. After the sampling is performed, the sampled agar culture medium is arranged in a warm box for cultivation and then is taken out for counting the colony formation number and performing analysis and evaluation. The sampler is manufactured simply and can sample all parts of a hand, the field sampling is convenient and samples are convenient to carry, and the disadvantage that the finger tip skin can only be sampled by adopting a conventional westem blot is overcome.
Description
Technical field
The utility model relates to a kind of syringe type agar impression sampling thief.
Background technology
At present, in the Disinfection Effect of hand, skin and body surface was measured, the method for sampling of use was a lot, and commonly used have cotton swab method, method of scoring, polybag method, elution method and a skin biopsy method etc. continuously.But there are defectives such as sampling is inconvenient, sample effect is not good mostly.
Summary of the invention
To above-mentioned prior art, the utility model provides a kind of syringe type agar impression sampling thief, and it makes simple, and spot sampling is easy to carry, samples in each position of ability adversary.
The utility model is realized through following technical scheme:
A kind of syringe type agar impression sampling thief comprises sampling pipe, is provided with pull bar in the sampling pipe, and sampling pipe is stretched out in the pull bar rear end, and the sampling pipe front end is provided with lid.
Said sampling pipe is provided with scale.
Said sampling pipe is cylinder type.The internal diameter of said sampling pipe is 25mm, and length is 12～15cm.
Use: earlier syringe type agar impression sampling thief is carried out sterilization treatment, be placed on then on the prior test tube rack or the special sampling thief rest stand made from ultraviolet radiation disinfection, the sampling pipe front end up; Pulling pull bar to sampling pipe internal pore length is about 10cm; Take off lid, pour sampling pipe into, build lid with the agar medium that sterile working will be dissolved; Treat its cooling, subsequent use.Sampling: in the time of need sampling to skin, get the syringe type agar impression sampling thief of recording, take off lid, touch pull bar, agar medium is released a little, with the sterilization scalpel top is scabbled then; And then release the about 3mm of agar medium, and press seal skin surface sample region with its end face, and rotate slightly, it is fully contacted with skin; Downcut the thick agar medium of 3mm that exposes with scalpel along the sampling pipe front end, the agar medium sampling of downcutting is faced up, place aseptic plate; And then release agar medium 3mm again, and another place's skin is sampled, so repeat.Cultivate: after the sampling, the agar medium upset of sampling is placed in 37 ℃ of incubators cultivates 48h, take out the counting bacterium colony and form number, and analyze and estimate.
The sampling thief rest stand structure of said special making is following: be the wooden shelf of long 450mm, wide 90mm; Yoke divides two-layer up and down; Two-layer dividing plate is apart from 100mm; Dividing plate has 8 diameter 35mm circular holes on the upper strata, and the groove of 8 diameter 35mm is arranged on corresponding lower floor's dividing plate, to place the usefulness of fixed sample device.
In the Disinfection Effect of hand, skin and body surface is measured, though the method for sampling of use is a lot, the trend basically identical that the Disinfection Effect of its demonstration is good and bad, the killing effect that therefore fixing a kind of method of sampling detects different sanitizers is feasible.The syringe type agar stamped method sampling of the utility model belongs to one of blotting, and test findings shows, with syringe type agar stamped method is sampled in the emgloves surface of artificial microbiological contamination, in bacterium amount≤5 * 10
2Individual/30cm
2In the scope, it is more accurate to adopt easy counting of the clump count that obtains and counting.
The syringe type agar impression sampling thief of the utility model is made simple, and spot sampling is easy to carry, each position sampling of ability adversary, and having overcome blotting commonly used can only be to the deficiency of digital tip skin sampling; Put into the direct 37 ℃ of cultivations of sterile petri dish after the impression sampling; Reduce cotton 2 of dilution, inoculations of wiping away semar technique and detected link; Avoided cotton to wipe away semar technique sampling back, made to adopt and obtain bacterium breeding in a large number in sample solution, and then cause the inaccurate situation of result if fail in time to cultivate; When adversary, skin and body surface natural bacteria were sampled,, bacterial contamination quantity clean relatively when the sampling position was not very under the serious situation, used the syringe type agar stamped method cotton of sampling to wipe away semar technique and can improve bacterium and detect the result.
Description of drawings
Fig. 1 is the structural representation of the utility model.
Wherein, 1, sampling pipe; 2, pull bar; 3, scale; 4, lid.
Embodiment
Below in conjunction with accompanying drawing the utility model is further described.
A kind of syringe type agar impression sampling thief comprises sampling pipe 1, and is as shown in Figure 1; Sampling pipe 1 is cylinder type, and the internal diameter of sampling pipe 1 is 25mm, and length is 12cm; Sampling pipe 1 is provided with scale 3; Be provided with pull bar 2 in the sampling pipe 1, sampling pipe 1 is stretched out in pull bar 2 rear ends, and sampling pipe 1 front end is provided with lid 4.
Use: earlier syringe type agar impression sampling thief is carried out sterilization treatment (double-deck infantees parcel carries out pressuresteam sterilization), be placed on then on the sampling thief rest stand of prior special making with ultraviolet radiation disinfection 30min; Sampling pipe 1 front end spurs pull bar 2 to sampling pipe 1 internal pore length and is about 10cm up, takes off lid 4; Agar medium with sterile working will be dissolved is poured sampling pipe 1 into; Build lid 4, treat its cooling, subsequent use.
The sampling thief rest stand structure of said special making is following: be the wooden shelf of long 450mm, wide 90mm; Yoke divides two-layer up and down; Two-layer dividing plate is apart from 100mm; Dividing plate has 8 diameter 35mm circular holes on the upper strata, and the groove of 8 diameter 35mm is arranged on corresponding lower floor's dividing plate, to place the usefulness of fixed sample device.
Sampling: when both hands are sampled, get the syringe type agar impression sampling thief of recording, take off lid 4, touch pull bar 2, agar medium is released a little, with sterilization scalpel (No. 4 handle of a knifes, No. 23 blades) top is scabbled then; And then release the about 3mm of agar medium, with its end face (about 4.9cm of nutrient culture media area
2) by seal skin surface sample region, and rotate slightly, it is fully contacted with skin; Downcut the thick agar medium of 3mm that exposes with scalpel along sampling pipe 1 front end, the agar medium sampling of downcutting is faced up, place aseptic plate; And then release agar medium 3mm again, and another place's skin is sampled, so repeat.Hand getting appearance 7 places, the about altogether 30cm of sampling area
27 agar mediums are put in the same plate.After a hand getting appearance end, change knife blade, again the another hand is sampled.The contained agar medium of sampling pipe can be used for the sampling to two hands.
Cultivate: after the sampling, the agar medium upset of sampling is placed in 37 ℃ of incubators cultivates 48h, take out the counting bacterium colony and form number.
Sample effect is estimated: adopting and obtain bacterium and be prone to counting and count results bacterium weight range more accurately for findding out stamped method, adopt artificial microbiological contamination method, is 4.6 * 10 with the bacterium amount
7(8099) 10 times of dilutions of successively decreasing of the Escherichia coli suspension of cfu/ml are got 1ml respectively and are inoculated in the palmar surface that experimenter's hand is worn sterilization back emgloves, with the cotton swab coating that is soaked with SPSS evenly, dry back impression sampling.
Result: with syringe agar impression sampling≤1/2 stoste * 10
5The bacterium amount of dilutability inoculation, sampling are after cultivate, and the bacterium amount is evenly distributed, and the naked eyes count results is comparatively desirable, and with count plate gradient basically identical (as shown in table 1).
The different dilutability bacteriums of table 1 impress sampled result through syringe type
Annotate: the result is 5 test mean values.
Conclusion: in the Disinfection Effect of hand, skin and body surface was measured, the method for sampling of use was a lot.Commonly used have cotton swab method, method of scoring, polybag method, elution method and a skin biopsy method etc. continuously.The method of sampling is different, and the killing rate that records is difference to some extent often, but the good and bad trend basically identical of the Disinfection Effect of its demonstration.The killing effect that therefore fixing a kind of method of sampling detects different sanitizers is feasible.The sampling of syringe agar stamped method belongs to one of blotting, and test findings shows, with syringe type agar stamped method is sampled in the emgloves surface of artificial microbiological contamination, in bacterium amount≤5 * 10
2Individual/30cm
2In the scope, it is more accurate to adopt easy counting of the clump count that obtains and counting.
Claims (4)
1. a syringe type agar impresses sampling thief, and it is characterized in that: comprise sampling pipe, be provided with pull bar in the sampling pipe, sampling pipe is stretched out in the pull bar rear end, and the sampling pipe front end is provided with lid.
2. syringe type agar impression sampling thief according to claim 1, it is characterized in that: said sampling pipe is provided with scale.
3. syringe type agar impression sampling thief according to claim 1, it is characterized in that: said sampling pipe is cylinder type.
4. syringe type agar impression sampling thief according to claim 3, it is characterized in that: the internal diameter of said sampling pipe is 25mm, length is 12～15cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011203304570U CN202188973U (en) | 2011-09-05 | 2011-09-05 | Injector type agar stamping sampler |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011203304570U CN202188973U (en) | 2011-09-05 | 2011-09-05 | Injector type agar stamping sampler |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202188973U true CN202188973U (en) | 2012-04-11 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011203304570U Expired - Fee Related CN202188973U (en) | 2011-09-05 | 2011-09-05 | Injector type agar stamping sampler |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202188973U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106350575A (en) * | 2016-08-31 | 2017-01-25 | 山东新华医疗器械股份有限公司 | Embossing method for bacterium sampling |
| CN107661124A (en) * | 2017-09-22 | 2018-02-06 | 陈昭斌 | A kind of skin degerming effect monitoring quick sampling device |
| CN108858293A (en) * | 2018-05-24 | 2018-11-23 | 福建农林大学 | Visual row's formula running gel rubber knife and its application method |
-
2011
- 2011-09-05 CN CN2011203304570U patent/CN202188973U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106350575A (en) * | 2016-08-31 | 2017-01-25 | 山东新华医疗器械股份有限公司 | Embossing method for bacterium sampling |
| CN107661124A (en) * | 2017-09-22 | 2018-02-06 | 陈昭斌 | A kind of skin degerming effect monitoring quick sampling device |
| CN108858293A (en) * | 2018-05-24 | 2018-11-23 | 福建农林大学 | Visual row's formula running gel rubber knife and its application method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120411 Termination date: 20120905 |