RU2519357C2 - Method for amniotic membrane dissection for intrastromal implantation into corneal stroma on ice-cold surface - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention refers to medicine, and may be used for amniotic membrane dissection. The amniotic membrane is distributed on an ice-cold surface until freezing down. A corneal trepan of the necessary diameter is used to dissect an amniotic membrane flap on the ice-cold surface.

EFFECT: higher effectiveness of the method.

2 dwg

Description

The invention relates to medicine, to the field of ophthalmology, and can be used for cutting a donor amniotic membrane intended for intrastromal implantation, for more accurate sparing cutting an implant from an amniotic membrane with the aim of a clear dosage of therapeutic effects.

The closest in technical essence and the achieved result is a method of cutting out a lyophilized donor amniotic membrane glued to nitrocellulose paper (Kim J. C., Tseng S.C.G. // Cornea. - 1995.-Vol. 14. - P. 473-484).

However, the known method has a significant drawback - the amniotic membrane is fixed on nitrocellulose paper, which does not separate during transplantation and is a foreign body, which does not allow the use of intrastromal implantation of an implant obtained from a lyophilized amniotic membrane. Lyophilization is not possible without special equipment.

A method is proposed for cutting an amniotic membrane for intrastromal implantation into the stroma of the cornea on an ice surface.

A native amniotic membrane is used. Amnion is evenly distributed on the ice surface until it freezes. Then a round section is cut out with a corneal trepan of the required diameter. The resulting membrane fragment is placed in a solution containing 40 mg / ml gentamicin and 2.5 mg / ml amphotericin. After a 20-minute exposure, the amniotic membrane flap can be used for implantation. In Fig. 1. The implant is cut out from an amniotic membrane flap on an ice surface. Figure 2 shows the finished implants of the amniotic membrane.

The method allows to reduce the morbidity of the operation of intrastromal amnion implantation, to exclude the occurrence of membrane folding in the stroma of the cornea during implantation, to reduce the possibility of mechanical damage and perforation of the implant when it is cut out.

Example

An implant from the amniotic membrane was made according to the proposed method and implanted intrastromally to patient N. with DS: Endothelial-epithelial dystrophy 3, artifactia OD. Visual acuity before surgical treatment: OD - 0.005 n.k. The graft diameter of the amniotic membrane was 8.5 mm. After the operation, a solution of Ciprofloxacin and a solution of Diclofenac were installed 6 times a day daily in the conjunctival cavity, and Korneregel was laid. The implant was located in the layers of the cornea evenly without folding, had clear edges. The edges of the surgical wound have been adapted. After the treatment, there was an increase in visual acuity from 0.005 to 0.03, relief of the corneal syndrome, completion of epithelization on days 8–9, the disappearance of bullous changes in the epithelium, and a decrease in corneal edema. The decrease in the degree of hydration of the corneal stroma primarily occurred in the projection of the implanted membrane.

Information sources

1. Gundorova, R. A. Application of the amniotic membrane in ophthalmology: a literature review // R. A. Gundorova, O. V. Kiseleva, N. V. Sorokoletova. // Refractive surgery and ophthalmology. - 2007. - No. 2. - S. 27-31.

2. Kasparov, A.A. The use of canned amniotic membrane for reconstruction of the surface of the anterior segment of the eye / A.A. Kasparov, St. Trufanov. // Vestn. Ophthalmol. - 2003. - No. 3. - S. 45-47.

Claims (1)

A method of cutting an amniotic membrane, characterized in that the amniotic membrane is distributed on the ice surface before freezing, after which a flap of the amniotic membrane on the ice surface is cut out with a corneal trepan of the required diameter.

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